[发酵芥子膏对支气管哮喘大鼠气道免疫平衡的影响及其炎症反应机制]。

Q3 Medicine
Jin-Xia Mu, Qing Zhang, Yu-Ming Qu, Tian-Sheng Zhang, Chong-Yao Hao, Kai-Nan Xiao
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引用次数: 0

摘要

目的:观察发酵白芥子膏对支气管哮喘(BA)大鼠T辅助细胞(Th) 1/Th2、Th17/T调节细胞(Treg)免疫平衡的影响,探讨发酵白芥子膏治疗BA的可能机制。方法:将健康SD大鼠随机分为空白组、模型组、给药组和穴位贴敷组,每组10只。采用卵清蛋白致敏雾化法制备BA模型。穴位贴敷组大鼠在“大椎”(GV14)、双侧“肺俞”(BL13)、“凤门”(BL12)处灌胃发酵白芥子膏4 h;给药组大鼠腹腔注射1 mg/kg地塞米松磷酸钠,两组大鼠均每日1次,连续14 d。干预前后对各组大鼠进行行为观察和评分。HE染色观察肺组织病理形态变化。采用ELISA法检测血清中干扰素γ (IFN-γ)、白细胞介素(IL)-4、IL-17、IL-10、肿瘤坏死因子α (TNF-α)、免疫球蛋白E (IgE)含量。采用实时荧光定量PCR检测肺组织中T-box转录因子(T-bet)、GATA结合蛋白3 (GATA-3)、视黄酸相关孤儿受体γt (RORγt)、叉头盒蛋白3 (Foxp3) mRNA表达水平。Western blot检测肺组织中T-bet、gada -3、rorr γt、Foxp3蛋白表达水平。结果:治疗后,与空白组比较,行为评分明显提高(ppppppppppppppppppppppp)。发酵白芥子膏可减轻BA气道炎症,其机制可能是通过上调T-bet和Foxp3的表达,下调gada -3和RORγt的表达,促进Th1/Th2、Th17/Treg免疫平衡,减少相关促炎因子的分泌,从而起到治疗哮喘的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effects of fermented white mustard seed plaster on airway immune balance and its inflammatory response mechanism in bronchial asthma rats].

Objectives: To observe the effects of fermented white mustard seed plaster on the balance of T helper cell (Th) 1/Th2, Th17/T regulatory cell (Treg) immune balance in bronchial asthma (BA) rats, so as to explore the possible mechanism of fermented white mustard seed plaster in the treatment of BA.

Methods: Healthy SD rats were randomly divided into blank, model, medication and acupoint application groups, with 10 rats in each group. BA model was prepared using ovalbumin sensitization and atomization. Rats in the acupoint application group were treated with fermented white mustard seed plaster at "Dazhui" (GV14), bilateral "Feishu" (BL13) and "Fengmen" (BL12) for 4 h;rats in the medication group were given intraperitoneal injection of 1 mg/kg dexamethasone sodium phosphate;both groups were treated once daily for 14 consecutive days. Behavioral observations and scoring were conducted on rats in each group before and after intervention. HE staining was performed to observe the pathological morphological changes of lung tissue. ELISA was used to detect the contents of interferon γ (IFN-γ), interleukin (IL)-4, IL-17, IL-10, tumor necrosis factor α (TNF-α), and immunoglobulin E (IgE) in serum. real-time quantitive PCR was used to detect the mRNA levels of T-box transcription factor (T-bet), GATA binding protein 3 (GATA-3), retinoic acid-related orphan receptor γt (RORγt), and forkhead box protein 3 (Foxp3) in lung tissue. Western blot was used to detect the protein expression levels of T-bet, GATA-3, RORγt, and Foxp3 in lung tissue.

Results: After treatment, compared with the blank group, the behavioral score was significantly increased (P<0.01);the thickness of bronchial wall muscle layer increased with a large amount of inflammatory cell infiltration, thickening of alveolar wall, abnormal lung tissue structure, and severe lung consolidation;the contents of serum IL-4, IL-17, TNF-α, and IgE were significantly increased (P<0.01), while the contents of serum IFN-γ and IL-10 were significantly decreased (P<0.01);the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were increased (P<0.01), while the mRNA and protein expression levels of T-bet and Foxp3 in lung tissue were decreased (P<0.01) of rats in the model group. Compared with the model group, the behavioral score was significantly decreased (P<0.01);the pathological morphological damage of lung tissue was significantly improved;the contents of serum IL-4, IL-17, TNF-α, and IgE were significantly decreased (P<0.01, P<0.05), while the contents of serum IFN-γ and IL-10 were significantly increased (P<0.01, P<0.05);the mRNA and protein expression levels of T-bet and Foxp3 in lung tissue were increased (P<0.01), while the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were decreased (P<0.01) of rats in the medication and acupoint application groups. Compared with the medication group, the contents of serum IL-4 were significantly increased (P<0.05);the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were significantly increased (P<0.01, P<0.05), while the mRNA and protein expression levels of T-bet and Foxp3 were significantly decreased (P<0.01, P<0.05) of rats in the acupoint application group.

Conclusions: Fermented white mustard seed plaster can alleviate airway inflammation in BA, and its mechanism may be achieved by up-regulating the expression of T-bet and Foxp3, down-regulating the expression of GATA-3 and RORγt, promoting Th1/Th2, Th17/Treg immune balance, and reducing the secretion of related pro-inflammatory factors to exert a therapeutic effect on asthma.

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来源期刊
针刺研究
针刺研究 Medicine-Medicine (all)
CiteScore
1.30
自引率
0.00%
发文量
0
期刊介绍: Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture. The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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