Journal of Chromatography B最新文献

{"title":"Bentonite modified with quaternary ammonium for enhanced human serum albumin adsorption","authors":"Saeed Barzegar ,&nbsp;Mehran Javanbakht ,&nbsp;Behrouz Akbari-Adergani","doi":"10.1016/j.jchromb.2025.124850","DOIUrl":"10.1016/j.jchromb.2025.124850","url":null,"abstract":"<div><div>Human Serum Albumin (HSA) is an important protein that helps regulate oncotic<!--> <!-->pressure and transport of diverse molecules. A high-performance adsorbent for the separation of albumin is strategic to improve efficiency and selectivity,<!--> <!-->which can result in a low-cost and simplified process for obtaining high-purity albumin. The present study aimed to elucidate the adsorption of HSA on modified bentonite and represent the adsorption capacity. Various characterizations confirm the modification and structural changes of bentonite after surface<!--> <!-->modification with dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DTSACL). A central composite design (CCD) for the response surface methodology (RSM) was used to investigate and optimize the effect of the pH, concentration of HSA, and sorbent dosage on the adsorption. The<!--> <!-->findings demonstrated that electrostatic interaction and functionalization of the surface were implemented to enhance the adsorption capacity of the modified bentonite. Thermodynamic investigations indicate an exothermic adsorption<!--> <!-->process and preferred ambient temperatures, while isothermal examinations confirm that monolayer adsorption was a better fit for the Langmuir model. The maximum adsorption capacity (q_e) of 430 mg /g was obtained with a desirability of 0.98 at optimized pH = 6.2,<!--> <!-->initial HSA concentration = 418.9 mg/L, and DTSACL-bentonite dosage = 30.1 mg in aqueous solution of HSA. The adsorption of HSA from real human serum samples achieved an adsorption efficiency of 79.8 % and a recovery rate of 92.5 %. High-performance liquid chromatography (HPLC) and SDS-PAGE analyses confirmed that DTSACL-bentonite exhibits high selectivity and efficiency, highlighting its potential as a promising adsorbent for the separation and purification of HSA.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124850"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145524974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a highly sensitive assay for the determination of rivastigmine in human plasma for pharmacokinetic studies.","authors":"Maksim Dolov ,&nbsp;Igor Rodin","doi":"10.1016/j.jchromb.2025.124838","DOIUrl":"10.1016/j.jchromb.2025.124838","url":null,"abstract":"<div><div>A fast and highly sensitive LC–MS/MS method has been developed and validated for measuring rivastigmine levels in human plasma. The extraction of rivastigmine from plasma was performed using a simple and quick protein precipitation technique (PPT). The internal standard used was atazanavir-d₅. Chromatographic separation was achieved using a YMC-Triart C18 column, followed by detection with mass spectrometry. The mass transitions monitored were <em>m</em>/<em>z</em> 251.1 &gt; 206.0 for rivastigmine, and m/z 710.5 &gt; 168.1 for atazanavir-d₅. This method involves rapid plasma extraction, straightforward gradient chromatography, and mass spectrometric detection, allowing for the detection of rivastigmine at sub-nanogram per milliliter levels. The method was validated over a linear range of 25 to 5000 pg/ml, with a correlation coefficient of at least 0.9980. Both intra- and inter-day precision and accuracy were within 15 %. The overall recovery rates for rivastigmine and atazanavir-d₅ were close to 100 %. The total analysis time per sample was only 3 min. This method was successfully applied to determine the pharmacokinetic parameters of rivastigmine after a single oral dose of 1.5 mg (capsules) in 26 healthy volunteers.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124838"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An LC-MS/MS method for simultaneous quantification of pyrotinib, docetaxel and paclitaxel in human plasma and its application to therapeutic drug monitoring","authors":"Lingxiao Zhang ,&nbsp;Jie Qu ,&nbsp;Xinyan Sun ,&nbsp;Haiyan Dong ,&nbsp;Hongping Yao ,&nbsp;Xiaoliang Cheng","doi":"10.1016/j.jchromb.2025.124840","DOIUrl":"10.1016/j.jchromb.2025.124840","url":null,"abstract":"<div><div>Pyrotinib which is a novel irreversible tyrosine kinase inhibitor plus docetaxel or paclitaxel is effective for patients with Her2 positive early or advanced breast cancer including those who failed in first-line treatment. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and verified for simultaneous quantification of pyrotinib, docetaxel and paclitaxel in human plasma, and applied to therapeutic drug monitoring. A reversed-phase Hypersil GOLD aQ column eluted by a gradient mobile phase composed of water and acetonitrile both containing 0.1 % formic acid under flow rate of 0.3 mL min⁻¹ was used for chromatographic separation. The mass spectrometry was operated in positive electrospray ionization mode, and selective reaction monitoring was applied for quantitative analysis. With imatinib as internal standard, one-step deproteinization approach with acetonitrile was applied to extract analytes and purify specimens. This method was adequately validated according to guidelines in terms of specificity and selectivity, sensitivity, linearity, extraction recovery, matrix effect, precision and accuracy, dilution integration and stability. The validated method was applied to therapeutic drug monitoring for breast cancer patients receiving pyrotinib and taxanes based chemotherapy. The therapeutic drug monitoring results showed that the plasma concentration of pyrotinib, docetaxel and paclitaxel varied significantly among individuals. Therapeutic drug monitoring for pyrotinib, docetaxel and paclitaxel is essential for individualized treatment to ensure efficacy and safety.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124840"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145440431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular imprinted solid-phase extraction and analysis of Entecavir in presence of its induced degradation products and co-administered drug(s) in spiked human plasma, environmental three-color assessment and sustainability profiling","authors":"Sarah S. Saleh,&nbsp;Heba T. Elbalkiny","doi":"10.1016/j.jchromb.2025.124843","DOIUrl":"10.1016/j.jchromb.2025.124843","url":null,"abstract":"<div><div>Entecavir (ETV) is an antiviral drug that acts by blocking the active viral replication process due to its chemical similarity to guanine. Through this study, the extraction of ETV samples was described, for the first time, using a synthesized molecular imprinted polymer solid phase extraction (MISPE). The MISPE was characterized using TEM and FTIR analysis. A green RP- HPLC method was developed for the quantitation of ETV using C18 column and a mobile phase consisting of 0.1 % phosphoric acid in water and methanol in a gradient mode delivered at a rate of 1 ml/min at room temperature. The UV detection was carried out at 245 nm. The analytical method was validated according to ICH guidelines with a linear range of (5–250 μg/mL). The specific extraction of ETV was carried out successfully using MISPE in presence of its induced acidic, and basic degradation products. MISPE was also used for the extraction of ETV from spiked human samples containing the co-administered drug lamivudine. The MISPE showed excellent selectivity, reusability and high recovery percentages (&gt;90 %) when compared to Oasis HLB cartridges. The analytical procedure was compared to the reported methods in terms of environmental three-color assessment (ETCA): greenness (using AGREE, AGREEMIP and ComplexMoGAPI), blueness (using BAGI), and whiteness (using RGB-12 algorithms). The proposed method transcended in saving energy, efficiency and applicability. The sustainability profile for the proposed method was established using the efficient-valid-green (EVG) framework displayed via its radar chart, that showed balance between the three pillars, and the NQS index that displays the excellent alignment of the method with the UN-17 SDGs.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124843"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of byakangelicin metabolites in rats via the UHPLC-Q-exactive orbitrap mass spectrometer","authors":"Ruijun Cai ,&nbsp;Zhongnan Mao ,&nbsp;Tao An ,&nbsp;Yunbo Zhang ,&nbsp;Li Zhou","doi":"10.1016/j.jchromb.2025.124841","DOIUrl":"10.1016/j.jchromb.2025.124841","url":null,"abstract":"<div><div>Byakangelicin is a furanocoumarin derived from the roots of Angelica dahurica. It exhibits pharmacological properties, including anti-tumor activity, as well as against liver injury and fibrosis. In this study, Ultra-High-Performance Liquid Chromatography with Q-Exactive Orbitrap Mass Spectrometry technology was employed to investigate the metabolic profile of byakangelicin in rats. Following intragastric administration the suspension of byakangelicin, plasma and tissue specimens were harvested. According to high-resolution extracted ion chromatograms, the metabolites of byakangelicin were identified by comparing accurate mass, diagnostic fragment ions, and chromatographic retention times. Data collection was performed in positive ion mode to facilitate metabolite characterization of byakangelicin. Overall, 46 metabolites were successfully characterised. The primary metabolic pathways included hydroxylation, dehydroxylation, methylation, demethylation, reduction, glucuronidation, glycination, and cysteinylation. This systematic investigation of the metabolic characteristics and pathways of byakangelicin in rats provides a valuable reference for future pharmacodynamic evaluations, pharmacological research, and drug development.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124841"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145497991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a LC-MS/MS method for the simultaneous quantitative determination of trimethylamine N-oxide and four precursors in human saliva","authors":"Jianyue Li ,&nbsp;Bin Hu ,&nbsp;Jie Wu ,&nbsp;Dazhen Wang ,&nbsp;Junyan Xia ,&nbsp;Yanan Li ,&nbsp;Yonghong Gao ,&nbsp;Baorong Chen ,&nbsp;Jianfeng Zhou ,&nbsp;Zenghe Li","doi":"10.1016/j.jchromb.2025.124829","DOIUrl":"10.1016/j.jchromb.2025.124829","url":null,"abstract":"<div><div>In this work, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously quantitatively detect the choline (CHL), trimethylamine N-oxide (TMAO), γ-butyrobetaine (GBB), betaine (BET), and ʟ-carnitine (CAR) in human saliva for the first time. Saliva samples collected from healthy volunteers were treated with acetonitrile for protein precipitation, followed by direct injection into the LC-MS/MS system for analysis. The method validation was performed in accordance with the CLSI C62-A standard guidelines. The linearity was in the range of 0.300–300.000 μmol/L for CHL, 0.005–5.000 μmol/L for TMAO, 0.050–50.000 μmol/L for GBB, 0.020–20.000 μmol/L for BET, and 0.100–100.000 μmol/L for CAR. The intra-day and inter-day precision ranged from 1.13 % to 8.34 % and from 1.63 % to 10.65 %, respectively. The recoveries and matrix effects of these analytes ranged from 94.31 % to 108.00 % and from 90.59 % to 111.37 %, respectively. The performance of the method met the requirements of the guidelines. The validated method was subsequently applied to analyze test saliva samples from 43 healthy volunteers and 4 coronary heart disease (CHD) inpatients, to evaluate the distributions of five analytes in healthy people and CHD inpatients. This study provides a reliable and accurate analytical approach for the clinical detection of five analytes in saliva.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124829"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-HRMS- and TLC-based metabolomics for the identification and authentication of Sida rhombifolia","authors":"Uswatun Hasanah ,&nbsp;Eti Rohaeti ,&nbsp;Irmanida Batubara ,&nbsp;Utami Dyah Syafitri ,&nbsp;Rudi Heryanto ,&nbsp;Taopik Ridwan ,&nbsp;Nancy Dewi Yuliana ,&nbsp;Mohamad Rafi","doi":"10.1016/j.jchromb.2025.124834","DOIUrl":"10.1016/j.jchromb.2025.124834","url":null,"abstract":"<div><div>Ensuring the authenticity and quality of <em>Sida rhombifolia</em> raw materials is crucial for its herbal medicinal product's consistent efficacy, quality, and safety. In this study, we developed an identification and authentication method for identifying and authenticating <em>S. rhombifolia</em> from <em>Turnera subulata</em>. <em>T. subulata</em> has the same leaf morphology as <em>S. rhombifolia,</em> so that it could be used as an adulteration raw material for <em>S. rhombifolia</em>. We employed liquid chromatography-high resolution mass spectrometry (LC-HRMS)- and thin-layer chromatography (TLC)-based metabolomics for that purpose. Distinct chemical fingerprints of <em>S. rhombifolia</em> from <em>T. subulata</em> were obtained using LC-HRMS and TLC fingerprint analysis. Mixtures of <em>S. rhombifolia</em> and <em>T. subulata</em> powdered samples at varying concentrations (5 %, 25 %, and 50 % <em>w</em>/w) were analyzed using principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to see differences of each group. The PCA score plot from the TLC analysis explained over 70 % of the total variance, while LC-HRMS data provided the highest classification accuracy at 97.05 %. This integrated approach enhances the reliability of <em>S. rhombifolia</em> authentication by combining TLC's rapid profiling capability with LC-HRMS's analytical precision. This study provides a robust analytical framework for the quality control of herbal medicines, specifically addressing challenges related to adulteration.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124834"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an LC-MS/MS method for Tirzepatide, a dual GIP/GLP-1 receptor agonist, in rat plasma for application to a pharmacokinetic study","authors":"Hae-In Choi ,&nbsp;Hyeon-Cheol Jeong ,&nbsp;Jong-Woo Jeong ,&nbsp;Jaeyoung Lee ,&nbsp;Da Hae Kim ,&nbsp;Kyong-Cheol Ko ,&nbsp;Yoon-Jee Chae ,&nbsp;Kyeong-Ryoon Lee","doi":"10.1016/j.jchromb.2025.124836","DOIUrl":"10.1016/j.jchromb.2025.124836","url":null,"abstract":"<div><div>A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of tirzepatide, a dual agonist of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) receptors in rat plasma. Tirzepatide was extracted from rat plasma by protein precipitation using methanol. Chromatographic separation was achieved using a peptide C18 column with gradient elution of water and acetonitrile containing 0.1 % formic acid. Mass spectrometric detection was performed in positive electrospray ionization mode using multiple reaction monitoring with transitions of <em>m</em>/<em>z</em> 1204.4 → 1473.6 for tirzepatide and m/z 1029.4 → 1238.4 for the internal standard, semaglutide. The developed method exhibited good linearity over a concentration range of 1–1000 ng/mL (r² &gt; 0.99). Intra- and inter-day accuracy (−4.324–5.057 %) and precision (5.250–9.000 %) met the regulatory criteria at all quality control levels, and were stable under various plasma handling and storage conditions. The validated method was successfully applied to a pharmacokinetic study in rats following the intravenous and subcutaneous injection of tirzepatide at 0.3 mg/kg. The terminal half-lives were 10.04 h and 9.803 h after intravenous and subcutaneous administration, respectively, indicating comparable elimination profiles. The bioavailability following subcutaneous dosing was estimated to be approximately 62.38 %. These findings highlight the robustness and applicability of the developed method, suggesting its potential utility for the quantitative analysis of other peptide therapeutics with structures or mechanisms of action similar to those of tirzepatide.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124836"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145461102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer enrichment strategy for the detection of erythropoietin-receptor agonists in blood samples: A potential alternative to antibody-based assays","authors":"Yeşim Somay Selbes","doi":"10.1016/j.jchromb.2025.124846","DOIUrl":"10.1016/j.jchromb.2025.124846","url":null,"abstract":"<div><div>Optimal detection of Erythropoietin receptor agonists (ERAs) in anti-doping analyses relies heavily on efficient immunopurification (IP) strategies. While antibody-based IP is routinely applied, aptamers have emerged as promising alternatives. This proof-of-principle study investigated the applicability of an anti-EPO DNA aptamer for ERA enrichment in serum, followed by Sarkosyl-Polyacrylamide Gel Electrophoresis (SAR-PAGE) analysis.</div><div>The aptamer-based method successfully captured recombinant ERA variants (rEPO, dEPO, EPO-Fc, CERA) with defined limits of detection (LOD) in serum: 10 mIU/mL for rEPO, 10 pg/mL for dEPO, and 25 pg/mL for EPO-Fc and CERA. In comparison, the antibody-based method achieved lower serum LODs of 5 mIU/mL for rEPO, 5 pg/mL for dEPO, and 12.5 pg/mL for EPO-Fc and CERA. Both methods demonstrated good selectivity, as no non-specific bands were detected in blank serum matrices. However, endogenous blood EPO (bEPO) was not enriched by the aptamer, likely reflecting glycosylation-related structural differences between endogenous and recombinant isoforms.</div><div>These findings highlight that aptamers can provide highly specific recognition of recombinant ERAs without cross-reactivity to bEPO, which may simplify interpretation in doping control analyses. Nonetheless, further optimization—such as refining immobilization chemistries, incorporating differently glycosylated EPO forms during SELEX, and sequence improvements—is required to enhance capture of native isoforms. Overall, this study establishes a foundation for aptamer-based enrichment as a complementary alternative to antibody-based methods in anti-doping applications.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124846"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145484533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cyclodextrin-deep eutectic solvent-based dispersive liquid-liquid microextraction method for quantitative determination of small molecules in fresh-cut Codonopsis Radix","authors":"Yao Ma ,&nbsp;Qing-nan Chen ,&nbsp;Rui Liao ,&nbsp;Yun-e Bai ,&nbsp;Jian-kuan Li ,&nbsp;Shuang Hu ,&nbsp;Jian-ping Gao","doi":"10.1016/j.jchromb.2025.124844","DOIUrl":"10.1016/j.jchromb.2025.124844","url":null,"abstract":"<div><div>In this study, a cyclodextrin-dispersive liquid-liquid microextraction-HPLC-based method was developed to quantitatively study four components of Codonopsis Radix (CR): carboxymethyl-<em>β</em>-cyclodextrin (CM-<em>β</em>-CD) was incorporated into the DES, then added to the sample solution, and the mixture was vortexed and centrifuged, the upper hydrophobic phase was then collected for HPLC analysis. The primary elements influencing the extraction efficiency were optimized to choose the circumstances for this experiment, including the type and consumption of cyclodextrin, the NaCl concentration, the pH level of the sample phase and the extraction time. The <em>EFs</em> for four target components extracted by cyclodextrin-DES were between 8.4 and 71.2, which were all higher than those extracted only by DES. In their respective linearity ranges, the four analytes exhibited good linearity (R² ≥ 0.99), with detection limits of 1.2 × 10⁻² μg/mL, 5.3 × 10⁻³ μg/mL, 3 × 10⁻⁴ μg/mL, and 1.1 × 10⁻⁴ μg/mL. The precision and accuracy of the method ranged from 0.8 % to 10.0 % and 90.0 % to 105.2 %. Finally, this method was applied to the quantification of fresh-cut CR and traditional-processed CR. The results showed that the content of tangshenoside I and lobetyolinin of fresh-cut CR was significantly higher than in traditionally processed CR, but there were no significant differences between fresh-cut CR and traditionally processed CR for lobetyolin and lobetyol. The use of CM-<em>β</em>-CD in combination with DES provides rapid, simple, and reliable quantification of the target analytes. By this method, differences between the two processing methods were compared at the level of small-molecule content, providing evidence for the feasibility of the fresh-cutting processing method and demonstrating a theoretical basis for the quality evaluation method of the fresh-cut process.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1268 ","pages":"Article 124844"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145508706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}