华支睾吸虫感染大鼠的绝对定量脂质组学揭示了代谢途径的关键改变

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-06-06 DOI:10.1016/j.jchromb.2025.124672

Zhenli Xu , Shiwen Hua , Jian Ding , Xinyi Hu , Rui Chen , Yang Cheng , Su Han

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引用次数: 0

摘要

由于与慢性肝胆疾病相关，华支睾吸虫感染是一个重要的公共卫生问题。然而，中华梭菌感染的机制及其对宿主代谢的影响尚不清楚。本研究在感染后4周和8周采集大鼠血清样本，采用超高效液相色谱-质谱联用绝对定量脂质组学技术分析脂质代谢产物。进行多变量分析以确定差异表达的脂质，并利用MetaboAnalyst和KEGG数据库进行途径分析。共鉴定出5个主要类别的12个脂质亚类，其中三酰基甘油（TAG）受到的影响最为显著。在4 wpi时，230种脂质上调，18种下调，而在8 wpi时，与对照组相比，88种脂质上调，73种下调。结果显示，脂质谱发生了显著变化，尤其是TAG、游离脂肪酸（FFA）和磷脂酰胆碱（PC），其中磷脂酰乙醇胺（PE）表现出最明显的动态变化。途径富集分析显示，不饱和脂肪酸的生物合成、α -亚麻酸代谢和甘油脂代谢参与了对感染的反应。本研究确定了中华弓形虫感染大鼠脂质组学的显著改变，揭示了关键代谢途径的关键扰动，这可能涉及宿主的免疫和炎症反应。这些发现不仅提供了潜在的治疗靶点，而且加深了我们对慢性感染中宿主-寄生虫代谢相互作用的理解。

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Absolute quantitative lipidomics of Clonorchis sinensis-infected rats reveals key alterations in metabolic pathways

Clonorchis sinensis (C. sinensis) infection is a significant public health concern due to its association with chronic hepatobiliary diseases. However, the mechanisms underlying C. sinensis infection and its effects on host metabolism remain poorly understood. In this study, serum samples were collected from rats at 4 and 8 weeks post-infection (wpi), and lipid metabolites were analyzed using absolute quantitative lipidomics with UHPLC-MS/MS. Multivariate analyses were conducted to identify differentially expressed lipids, and pathway analysis was performed utilizing the MetaboAnalyst and KEGG databases.

A total of 12 lipid subclasses across five major categories were identified, with triacylglycerols (TAG) being the most significantly affected. At 4 wpi, 230 lipids were up-regulated and 18 down-regulated, whereas at 8 wpi, 88 were up-regulated and 73 down-regulated compared to controls. The results revealed significant alterations in lipid profiles, particularly in TAG, free fatty acids (FFA), and phosphatidylcholine (PC), with phosphatidylethanolamine (PE) exhibiting the most pronounced dynamic changes. Pathway enrichment analysis revealed significant involvement of the biosynthesis of unsaturated fatty acids, alpha-linolenic acid metabolism, and glycerolipid metabolism in response to the infection.

This study identifies significant alterations in the lipidomics of C. sinensis-infected rats, revealing critical perturbations in key metabolic pathways, which are likely involved in the host's immune and inflammatory responses. These findings not only offer potential therapeutic targets, but also deepen our understanding of host-parasite metabolic interactions during chronic infection.

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.