Phytomedicine Plus最新文献

{"title":"Determination of analgesic potential of Helicteres isora and molecular involvement of opioids and GABA receptors","authors":"Fakhria A. Al-Joufi ,&nbsp;Hayat Bilal ,&nbsp;Mubarak Ali Khan ,&nbsp;Syed Muhammad Tahir Shah ,&nbsp;Syed Muhammad Mukarram Shah ,&nbsp;Muhammad Zahoor ,&nbsp;Syed Wadood Ali Shah","doi":"10.1016/j.phyplu.2025.100766","DOIUrl":"10.1016/j.phyplu.2025.100766","url":null,"abstract":"<div><div>Herbal medicines are used as a source of therapeutic agents as they are safer and less toxic than synthetic drugs. Several species of <em>Helicteres isora</em> (Sterculiaceae) have been documented in folk medicine to have analgesic properties. This study is aimed to explore possible analgesic potential of <em>Helicteres isora. linn</em> crude extracts (Hi-Crd) and its sub-fractions, ethyl acetate (Hi-Et) and chloroform (Hi-Chl) by using various models for pain such as acetic acid induced writhing model, formalin paw licking test and tail immersion test. A significant decrease was seen in the frequency of writhing in acetic acid-induced writhing model. The crude extracts and chloroform fractions were more effective when compared to standard. The negative control group displayed a writhing frequency of 76.56 ± 1.21, Hi-Crd at dose of 300 mg/kg and Hi-Chl at dose of 75 mg/kg produces writhing of 20.18 ± 2.03 (P &lt; 0.001) and 17.74 ± 2.39 (P &lt; 0.001) respectively. In formalin paw licking model Crude extract at a dose of 300 mg/kg b.w produced licking response of 20.09 ± 1.45 (P &lt; 0.001) in Phase-I with percentage activity of 49.85% and 17.22 ± 1.33 (P &lt; 0.001) in Phase-II with percentage activity of 72.15 %. Hi-Chl at a dose of 75 mg/kg b.w produced licking response of 19.55 ± 1.76 (P &lt; 0.001) in Phase-I with percentage activity of 51.19 % and 16.01 ± 2.21 (P &lt; 0.001) in Phase-II with percentage activity of 74.11 %. The central analgesic potential was determined by tail immersion test in which the Hi-Chl fraction was most potent and delivered percentage activity of 71.64 %. The standard tramadol delivered percentage activity of 82.29 % at a dose of 10 mg/kg. For the involvement of opioid receptors naloxone was co-administered with standard and test sample at a dose of 2 mg/kg, naloxone completely antagonized tramadol. The analgesic effects of Hi-Crd, Hi-Chl and Hi-Et were completely reversed by naloxone, this confirms involvement of opioids receptors in the analgesic effect of the extracts. For the involvement of GABAergic system bicuculline was injected prior to treatment with test samples. The writhing in the absence and in the presence of bicuculline were partially reversed and the involvement of GABAergic system in the analgesic effect of the crude extracts and fractions is testified.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100766"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of possible antimalarial constituent(s) from the leaves of Ochna kibbiensis: A phytochemical, in vivo and in silico approaches","authors":"A.J. Yusuf ,&nbsp;M.I. Abdullahi ,&nbsp;I. Nasir ,&nbsp;A. Yunusa ,&nbsp;A.M. Musa ,&nbsp;A.A. Muhammad ,&nbsp;A.E. Adegboyega","doi":"10.1016/j.phyplu.2025.100764","DOIUrl":"10.1016/j.phyplu.2025.100764","url":null,"abstract":"<div><h3>Background</h3><div>Malaria is a major public health problem especially in developing countries such as Nigeria and its treatment is being compromised by resistance of the parasite to most of the available drugs, including the current gold-standard artemisinins. <em>Ochna kibbiensis</em> Hutch. &amp; Dalziel, a synonym of <em>Ochna staudtii</em> Gilg has been employed in ethnomedicine for the treatment of malaria, dysentery, among others.</div></div><div><h3>Purpose of the study</h3><div>This study was aimed at identifying possible antimalarial constituents from <em>O. kibbiensis</em> leaves using phytochemical and <em>in silico</em> approach.</div></div><div><h3>Methods</h3><div>The antimalarial effect of the methanol leaf extract and its fractions (hexane, dichloromethane, ethylacetate, and butanol) was investigated according to the methods described by Ryley and Peters suppressive, and curative test using Chloroquine-sensitive <em>Plasmodium berghei</em> (NK65). <em>In silico</em> studies was conducted using on Schrödinger Suite 2021–2 utilizing the Glide-Ligand Docking panel of Maestro 12.8, SwissADME and ProTox-II online serves.</div></div><div><h3>Results</h3><div>The extract and fractions exhibited significant (<em>p</em> <em>&lt;</em> <em>0.05</em>) suppression of parasitaemia at 500, 250 and 125 mg/kg, respectively; butanol fraction was the most active with 94.0, 98.0 and 84.0 % chemo-suppression while the methanol extract was the least active with 32.5, 40.0 and 62.0 % chemo-suppression and the standard drug, chloroquine (CQ, 5 mg/kg) had 94.0 %. Also, the butanol fraction showed significant (<em>p</em> <em>&lt;</em> <em>0.05</em>) and dose-dependent effect in the curative test with 58.14, 74.40 and 94.20 % cure and CQ had 69.80 % at the tested doses while n-hexane fraction was the least active with 41.9, 14.0 and 2.3 % cure. Encouraged by these results, the butanol fraction was subjected to purification using chromatographic techniques which afforded <em>4</em>′-O-(2E-decenyl) rutin or <em>4</em>′-O-(2E-decenyl) quercetin-3-rutinoside as the major bioactive constituent. The identity of the compound was determined on the basis of chemical tests, and NMR analysis. The compound exhibited good binding affinity (-6.822 kcal/mol) and stability against <em>pf</em>LDH with appropriate and acceptable drug-like abilities and safety profile.</div></div><div><h3>Conclusion</h3><div>In conclusion, the leaf of <em>O. kibbiensis</em> have demonstrated significant antimalarial effect with a flavonol diglycoside as the major bioactive compound. To the best of our knowledge, this compound is isolated for the first time from the plant.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100764"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aegeline: A biologically important alkaloid from nature","authors":"Nitin A. Lunagariya ,&nbsp;Purvi Shah","doi":"10.1016/j.phyplu.2025.100765","DOIUrl":"10.1016/j.phyplu.2025.100765","url":null,"abstract":"<div><div>Aegeline, a naturally occurring alkaloid isolated from Aegle marmelos, has garnered significant attention for its diverse pharmacological properties. This review provides insights into the isolation, total synthesis, and biological activities of aegeline. Its potential as a therapeutic agent for various metabolic disorders, is highlighted with its mechanism of action. Aegeline is naturally available in racemic form. Mostly studies have been done on the isolated aegeline from the plant. Synthetic derivatives or analogues, which have been studied for biological activity, are also not specified for their chirality. Further studies can be done to explore therapeutic potential of its individual enantiomers along with the safety profile.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100765"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drying as a preservation strategy for medicinal plants: Physicochemical and functional outcomes for food and human health","authors":"Sarthak Nakra,&nbsp;Soubhagya Tripathy,&nbsp;Prem Prakash Srivastav","doi":"10.1016/j.phyplu.2025.100762","DOIUrl":"10.1016/j.phyplu.2025.100762","url":null,"abstract":"<div><div>Drying is one of the most commonly employed preservation techniques for medicinal plants, significantly influencing their physicochemical and functional properties. This review investigates the impact of various drying methods on the chemical composition, bioactive compounds, antioxidant activity, and overall quality of medicinal plants. These changes can diminish the therapeutic potential of the plants, thereby limiting their effectiveness in food applications and human health remedies. However, certain drying techniques are shown to better preserve these functional properties. The review discusses the integration of dried medicinal plants into functional foods, nutraceuticals, and supplements, showcasing their potential in promoting health and preventing diseases. The industrial significance of these approaches is in their capacity to prolong the period that a product can be stored, guarantee the safety of food, and satisfy consumers' desires for items derived from nature. Nevertheless, there are still obstacles to achieving a harmonious equilibrium between cost-efficiency, scalability, and environmental sustainability. By understanding the effects of different drying methods, this review aims to guide future research and industrial applications for maximizing the health benefits of medicinal plants.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100762"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect OF M. koenigii on the expression of cell wall formation related genes (mecA and fmhb)","authors":"Ibrahim Abuga ,&nbsp;Mohammad Syaiful Bahari Abdull Rasad ,&nbsp;Sulaimon Olayiwola Folami","doi":"10.1016/j.phyplu.2025.100757","DOIUrl":"10.1016/j.phyplu.2025.100757","url":null,"abstract":"<div><h3>Introduction</h3><div>Generally, quantitative polymerase-chain-reaction technique (qPCR) has been recognized as the gold standard for gene expression analysis. It supersedes the original conventional PCR, due to its ability of measuring the amplification of cDNA in real time as the reaction progresses.</div></div><div><h3>Aims</h3><div>The aim of this study was to determine the effects of the ethyl acetate leaf extract of <em>Murraya koenigii</em> (<em>M. koenigii</em>) on the expression of cell wall formation related genes (<em>mecA</em> and <em>fmhb</em>).</div></div><div><h3>Materials and Methods</h3><div>Ribonucleic acid (RNA) of the bacterial cells (<em>Staphylococcus aureus</em> ATCC 700,699) was extracted using Trizol reagent. The concentrations and purities of all RNA analysis were obtained from NanoDrop Spectrophotometer. cDNA Synthesis Kit was used for cDNA synthesis. The integrity of the cDNA was identified using ethidium bromide, through 1.5% agarose gel electrophoresis in 1 x TBE buffer. Finally, quantitative real-time PCR technique was employed to establish and validate the antibacterial activity of the plant extract on gene expression of the selected genes at the cellular level and the quantification of the gene's expression was determined using delta-delta Ct method.</div></div><div><h3>Result</h3><div>The result revealed that the exposure of the bacterial cells to the plant extract instigated upregulation of the selected genes. This indicates resistance of the bacteria to the treated extract against the selected cell wall formation genes</div></div><div><h3>Conclusion</h3><div>These findings suggest that the ethyl acetate leaf extract of <em>M. koenigii</em> lacks potential antibacterial activity on the expression of cell wall formation related genes (<em>mecA</em> and <em>fmhb</em>) of <em>S. aureus</em> bacteria.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100757"},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive phytochemical compounds characterization, anti-oxidant and anti-microbial activity of the methanol and acetonitrile leaf extracts of Pithecellobium dulce","authors":"M. Kiranmayee,&nbsp;K. Riazunnisa","doi":"10.1016/j.phyplu.2025.100760","DOIUrl":"10.1016/j.phyplu.2025.100760","url":null,"abstract":"<div><h3>Background</h3><div>Numerous natural substances with various pharmacological properties can be discovered in medicinal plants. <em>Pithecellobium dulce</em> (Roxb.) Benth is a traditional medicinal plant, belonging to the family Leguminosae. The various parts of the <em>P. dulce</em> plant are traditionally used to treat a wide range of health disorders in numerous countries.</div></div><div><h3>Purpose</h3><div>The aim of this investigation is to demonstrate the phytochemical profiling of methanol and acetonitrile leaf extracts of <em>P. dulce</em> and evaluate their antioxidant activity and antibacterial potential against pathogenic bacteria.</div></div><div><h3>Materials and methods</h3><div>The methanol and acetonitrile extracts of <em>P. dulce</em> leaf were extracted and characterized by using GCMS– and FTIR. Further evaluation was done for an understanding of the biomedical uses of <em>P. dulce</em>. The antibacterial activity of the extracts was monitored against <em>Escherichia coli, Bacillus subtilis</em>, and <em>Staphylococcus aureus</em>. The antioxidant activity of extracts was conducted by using DPPH and hydrogen peroxide scavenging activity, and total antioxidant activity was performed by phosphomolybdenum assay.</div></div><div><h3>Results</h3><div>A total of twenty-three compounds were tentatively identified through GC–MS; five of these were associated with the methanolic extracts, while eighteen were with the acetonitrile extract. The tentatively identified compounds present in acetonitrile leaf extract were 2-nonynoic acid; pentane, 2,4-dimethyl-; lupeo; phytol; propiolic acid; bicyclo(2.2.1), and heptane-5-(ethyl-1-amine). Propanamide; N-methyl-2-amino-; ethane, 1‑chloro-1-fluoro; nitrous oxide; oxirane, (ethoxymethyl)- and 2,5-cyclooctadien-1-ol were the compounds found in methanol extract. FTIR analysis identified various functional groups in the leaf extracts, indicating the presence of alcohols, phenols, unsaturated and saturated hydrocarbons, amines, flavonoids, and halogenated compounds. Acetonitrile and methanolic <em>P. dulce</em> leaf extracts exhibited significant antioxidant and antibacterial properties. Methanolic and acetonitrile leaf extracts exhibited the highest zone of inhibition against <em>E. coli</em> (21 ± 1.1 mm) and <em>B. subtilis</em> (20.6 ± 0.5 mm).</div></div><div><h3>Conclusion</h3><div>This study highlighted the presence of different phytochemicals that showed anti-bacterial and antioxidant activities. Therefore, <em>P. dulce</em> leaf extracts may play a significant role in inhibiting human infections.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100760"},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing global research on Datura stramonium L.: Integrative in vitro antifungal, antioxidant, and in silico molecular docking studies","authors":"Ankit kale ,&nbsp;Deepshikha Patle ,&nbsp;Shyam Ingle ,&nbsp;Durgesh M. Agase","doi":"10.1016/j.phyplu.2025.100763","DOIUrl":"10.1016/j.phyplu.2025.100763","url":null,"abstract":"<div><h3>Background</h3><div>Plant-based drugs are vital in modern medicine due to their effectiveness, lower side effects, and potential in treating chronic diseases and infections. <em>Datura stramonium</em> L.<em>,</em> a plant long recognized for its therapeutic properties, was investigated in this study to further explore its potential antioxidant and antifungal effects.</div></div><div><h3>Methodology</h3><div>The study aims to optimize ethanolic extracts from <em>D. stramonium</em> L. leaves to evaluate their antifungal and antioxidant properties using Disc diffusion method and DPPH free radical scavenging assay. Additionally, the bioactive compounds within the extracts were identified through GC–MS analysis and subsequently screened using molecular docking studies by docking the identified constituents with selected proteins of fungus <em>candida albicans.</em></div></div><div><h3>Result</h3><div>The antifungal activity of the extract was tested against the fungus <em>C. albicans ATCC10231</em> strain using a disc diffusion assay, with fluconazole serving as the positive control for determining the MIC. The extract exhibited maximum potency at 250 µg/mL and an MIC of 50 µg/mL. In the DPPH scavenging assay, the ethanolic leaf extract of <em>D. stramonium</em> L. demonstrated an IC₅₀ value of 146.69 ± 8.46 μg/mL. GC–MS analysis identified two major constituents: Heneicosane and Heptadecane, 2,6,10,15-tetramethyl. These compounds were further evaluated via molecular docking studies. Docking simulations with mannose-binding lectin proteins (1IYL, 1AI9) and aspartic protease proteins (1ZAP, 2H6S) of <em>C. albicans</em> showed that both phytoconstituents effectively bound to the target proteins, with the highest binding affinity observed for the 1ZAP protein.</div></div><div><h3>Conclusion</h3><div>The findings of this study demonstrate the significant antifungal activity of <em>D. stramonium</em> L. against the <em>ATCC10231</em> strain of <em>C. albicans</em>, along with notable antioxidant effects, likely attributed to the identified phytoconstituents. These results suggest a promising potential for synthesizing derivatives to enhance the efficacy of these bioactive compounds.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100763"},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of analgesic and anthelmintic activities of Artocarpus chaplasha ROXB. leaves supported by in silico molecular docking","authors":"S M Sohag ,&nbsp;Sharmin Nur Toma ,&nbsp;Md. Niaj Morshed ,&nbsp;Md. Al Imran Imon ,&nbsp;Md. Monirul Islam ,&nbsp;Md. Ibnul Piash ,&nbsp;Naznin Shahria ,&nbsp;Imran Mahmud","doi":"10.1016/j.phyplu.2025.100761","DOIUrl":"10.1016/j.phyplu.2025.100761","url":null,"abstract":"<div><div><em>Aartocarpus chaplasha</em> is a medicinal tree native to tropical regions, valued for its diverse therapeutic properties and bioactive compounds found in its leaves, bark, and fruit. This study investigates the analgesic and anthelmintic properties of the ethanolic leaf extract of <em>Aartocarpus chaplasha</em> (ELAC). HPLC analysis identified key compounds in ELAC, including catechin hydrate, epicatechin, rutin hydrate, p-coumaric acid, rosmarinic acid, quercetin, and kaemferol. ADMET properties and molecular docking analysis were performed using the identified compounds and analgesic targets COX-1 and COX-2 and the anthelmintic target tubulin-colchicine. Analgesic activity was assessed using the acetic acid-induced writhing test on Swiss albino mice, while anthelmintic effects were evaluated against <em>Paramphistomum cervi. In silico</em> molecular docking suggested that catechin hydrate and kaempferol could be promising alternatives for analgesic activity due to their higher binding energy with COX-1 and COX-2. Moreover, rutin hydrate showed strong binding energy with tubulin-colchicine against anthelmintic properties. At a 500 mg/kg dosage, ELAC exhibited significant analgesic effects. In the anthelmintic tests, a 100 mg/mL concentration resulted in the lowest paralysis and death times of 14.03 and 18.03 mins, respectively. Our present study suggests that <em>Aartocarpus chaplasha</em> leaves can be a potential source of lead compounds for pain management and helminth infections and further advanced investigations are required in the future.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100761"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of Red and White Ginger (Zingiber officinale) Rhizomes and Gingerol improves male reproductive capacities","authors":"Monday Obianke Ebeye ,&nbsp;Bartholomew Chukwuebuka Nwogueze ,&nbsp;Obidike Alexender Naiho ,&nbsp;John Chukwuka Igweh","doi":"10.1016/j.phyplu.2025.100758","DOIUrl":"10.1016/j.phyplu.2025.100758","url":null,"abstract":"<div><h3>Introduction</h3><div>The use of medicinal plants in herbal medicine is increasing steadily, and the beneficial medical effects of plant materials have recently been the subject of many scientific research. These effects generally result from the combination of secondary products present in the plants. <em>Zingiber officinale,</em> one of the many herbs with great potential, was used in this study. The purpose of this study was to determine the effect of red and white ginger (<em>Z. officinale</em>) rhizomes and 6 gingerol on the reproductive capacities of male Wistar rats.</div></div><div><h3>Materials and methods</h3><div>Seventy male rats were purchased and used in the study. The animals were randomly distributed into ten groups of seven rats, each group depending on the nature of the treatment with ginger rhizome. All animals were fed with normal rat chow and water. All the experimental rats were treated for four weeks. At the end of the treatment period, the experiment rats were humanely euthanized and semen was collected for sperm count, motility, viability, epididymal volume and morphology, while plasma from whole blood was collected for hormonal assays.</div></div><div><h3>Results</h3><div>Findings from our study showed that red and white ginger <em>Z. officinale</em> rhizomes and gingerol compound caused significant (<em>p</em> &lt; 0.05) increase in the body weights of rats compared to the control group. Similarly, there was a significant (<em>p</em> &lt; 0.05) increase in the relative testicular and epididymal organ weights, as well as a significant (<em>p</em> &lt; 0.05) increase in the sperm count, motility, viability, and epididymal volume with a corresponding reduction in abnormal sperm morphology was observed. Administration of red and white <em>Z. officinale</em> rhizomes and gingerol produced significant (<em>p</em> &lt; 0.05) increase in the levels of Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and testosterone, respectively. In addition, there was a significant (<em>p</em> &lt; 0.05) increase in the testosterone/LH ratio in experimental rats compared to the control group.</div></div><div><h3>Conclusion</h3><div>Bioactive compound derivatives of <em>Z. officinale</em> rhizomes (Red and white ginger) and 6 gingerol acts as a potent fertility promoters and are apparently safe for used in pregnancy, enhancing male reproductive success.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100758"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer potential of Syzygium cumini and Syzygium Jambolanum extracts against MCF-7 cell line: An in vitro evaluation","authors":"Sheikh Muhammad Suhail ,&nbsp;Amit Anand ,&nbsp;Abu Safana Biswas ,&nbsp;Santhepete Nanjundiah Manjula ,&nbsp;Kenganora Mruthunjaya","doi":"10.1016/j.phyplu.2025.100753","DOIUrl":"10.1016/j.phyplu.2025.100753","url":null,"abstract":"<div><h3>Background</h3><div>This research has been carried out to evaluate the anticancer activity of hydroalcoholic extracts of <em>Syzygium cumini</em> and <em>Syzygium jambolanum</em> against human MCF-7 cells .</div></div><div><h3>Purpose</h3><div>This study aims to identify the bioactive compounds present in the extracts, assess their <em>in vitro</em> antioxidant and anticancer potential, along with the possible mechanism of action for its anticancer property.</div></div><div><h3>Methods</h3><div>Hydroalcoholic extracts of <em>S. cumini</em> and <em>S. jambolanum</em> fruit pulp, Juice, Seed was subjected to GC–MS to identify its chemical compositions followed by invitro antioxidant cytotoxicity study by DPPH, MTT assay respectively. Further the promising extract was tested for Flow cytometric analysis. Apoptosis and qRT-PCR studies to investigate the mechanism of anticancer. Results were analyzed with GraphPad Prism 8 software and calculated as mean±SEM, p-value of &lt;0.05 was considered to be statistically significant.</div></div><div><h3>Results</h3><div>GC–MS analysis of hydroalcoholic extracts of <em>Syzygium cumini</em> and <em>Syzygium Jambolanum</em> (Fruit Pulp, Seed, Juice) found 113 compounds &amp; indicated significant amounts of n-hexadecanoic, tridecanoic, and dodecanoic acids. Among the extract <em>S. cumini</em> seed extract showed considerable antioxidant activity and the IC₅₀ value 45 μg/ml though both <em>S.C</em> &amp; <em>S.</em>J H.C extracts of Fruit pulp, Juice had and marked Cytotoxicity activity against MCF-7 cells with IC₅₀ value 80.75 μg/ml. Further mechanistic studies revealed that both the <em>S.C &amp; S.</em>J seed extracts showed cell cycle arrest in S phase and caused induction of apoptosis, via the increased, of p53 by western blotting studies.</div></div><div><h3>Conclusion</h3><div>The findings suggest that among hydroalcoholic extracts of <em>S. cumini</em> and <em>S. jambolanum.</em> S. cumini possess potential anticancer properties. Further studies can be warranted to explain the mechanisms of action and explore their therapeutic applications.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100753"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}