Phytomedicine Plus最新文献

{"title":"Immunomodulatory and antiviral properties of herbal formulation: Evidence from clinical trials in healthy volunteers, COVID-19 patients and molecular docking studies","authors":"Sarvesh Kumar Singh ,&nbsp;Kshipra Rajoria ,&nbsp;Sanjeev Sharma ,&nbsp;Sudhir Dadram Patsute ,&nbsp;Suchitra Ganeshacharya ,&nbsp;Sarita Singh ,&nbsp;William Rananaware ,&nbsp;Sandeep Kumar Thakur ,&nbsp;Pawan Kumar Singh","doi":"10.1016/j.phyplu.2025.100917","DOIUrl":"10.1016/j.phyplu.2025.100917","url":null,"abstract":"<div><h3>Aim</h3><div>This study aimed to evaluate the immunomodulatory and antiviral potential of herbal formulation through clinical trials in healthy volunteers and COVID-19 patients, complemented by <em>in-silico</em> molecular docking against viral target proteins.</div></div><div><h3>Methods</h3><div>The first single-blind, randomized, parallel-arm, controlled trial included 90 healthy participants allocated to three groups: Herbal Formulation (HF-1, HF-2), and Placebo (P-1). Each group received 10 mL of the respective intervention twice daily for 30 days. Based on favourable outcomes with HF-2, a second was randomized, double-blind, placebo-controlled clinical trial conducted in 60 patients with mild-to-moderate COVID-19, randomized to receive either HF-2 or placebo for 10 days, with evaluations on days 0, 4, 7, and 10. Additionally, molecular docking of phytoconstituents from the formulation was performed against viral proteins from HSV, influenza virus, lymphocytic choriomeningitis virus (LCMV), parainfluenza virus, rhinovirus, and SARS-CoV-2.</div></div><div><h3>Results</h3><div>In the healthy volunteers trial, HF-2 significantly enhanced CD⁴⁺ and CD⁸⁺ T-cell counts, alongside a reduction in C-reactive protein (CRP) levels in 77% of subjects, compared to 57% and 63% in HF-1 and placebo groups, respectively. In COVID-19 patients, HF-2 significantly improved recovery rates and decreased inflammatory markers including CRP, LDH, and IL-6, without reported adverse events. Docking studies revealed that HF-2 phytochemicals demonstrated strong binding affinities with viral proteins (VP1, neuraminidase, hemagglutinin, glycoproteins, Mpro, HN complex, and DNA polymerase), suggesting inhibition of viral invasion and replication.</div></div><div><h3>Conclusion</h3><div>HF-2 exhibited robust immunomodulatory and antiviral effects, validated by both clinical and <em>in-silico</em> findings. These results highlight its potential as a safe adjuvant therapy for viral infections and justify further large-scale clinical investigations.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100917"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excavation and validation of the active components and mechanisms of longan against colorectal cancer","authors":"Shilian Huang ,&nbsp;Yang Li ,&nbsp;Jinqing Huang ,&nbsp;Shaofeng Jiang ,&nbsp;Dongliang Guo","doi":"10.1016/j.phyplu.2025.100924","DOIUrl":"10.1016/j.phyplu.2025.100924","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Backgound&lt;/h3&gt;&lt;div&gt;Colorectal cancer (CRC) is the fourth most common malignant tumor worldwide and remains a leading cause of cancer-related mortality. Dimocarpus longan Lour., a member of the Dimocarpus genus in the Sapindaceae family, has been traditionally used as a tonic to alleviate deficiencies and enhance cognitive function. In addition, its extracts exhibit therapeutic potential against various malignant tumors. However, no studies have reported the potential role of longan in CRC treatment.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Study design&lt;/h3&gt;&lt;div&gt;Metabolomics was used to detect secondary metabolites of longan, while network pharmacology was used to explore its active components and potential mechanisms of action against CRC. Finally, bioinformatics analysis, molecular docking, and cellular experiments were conducted to further validate the findings.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;This study used metabolomics integrated with network pharmacology to qualitatively and quantitatively analyze the flesh of five developmental stages of ‘Shixia’ eye fruit. The potential active ingredients, core targets, and mechanisms of action of longan against CRC were identified, and the expression of core targets in CRC was validated at multiple levels using cBioPortal, CCLE database, Protein Atlas database, and GEPIA database. Use Kaplan Meier Plotter database and pROC software package to analyze the prognostic value and diagnostic significance of core targets. Molecular docking validation was performed using AutoDock Vina 1.2.2. Finally, the inhibitory effect of key compounds on HCT116 cells and their impact on EGFR and P53 protein expression were verified through MTT and Western blot experiments.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Luteolin, laricitrin, naringenin, hispidulin and diosmetin were hypothesized to be the key active compounds, while TP53, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), interleukin 6 (IL-6), epidermal growth factor receptor (EGFR), and AKT1 were identified as the probable key targets. TP53 is closely associated with CRC survival and prognosis, while IL-6 and GAPDH are potential diagnostic biomarkers for the disease. Molecular docking analysis revealed that TP53 and EGFR demonstrated strong binding affinity with lignans and geraniol, the key active compounds of longan. Cellular experiments indicated a significant reduction in cell viability following treatment, along with a decrease in EGFR levels and an upregulation of TP53 expression in HCT116 cells.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;Overall, longan exerts its anti-CRC effects through multiple targets, active components, and signaling pathways. These findings provide valuable insights into the potential therapeutic applications of longan as a traditional Chinese medicine resource.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Graphic abstract&lt;/h3&gt;&lt;div&gt;This study used ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to qualitatively and quantitatively analyze t","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100924"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oro-nasal film spray with andrographolide blocks SARS-CoV-2 and influenza infections and strengthens mucosal innate immunity","authors":"Wipawee Nittayananta ,&nbsp;Nopporn Chutiwitoonchai ,&nbsp;Hatairat Lerdsamran ,&nbsp;Aornrutai Promsong ,&nbsp;Teerapol Srichana ,&nbsp;Kesinee Netsomboon ,&nbsp;Suteema Sawadpongpan ,&nbsp;Jaruta Kerdto","doi":"10.1016/j.phyplu.2026.100957","DOIUrl":"10.1016/j.phyplu.2026.100957","url":null,"abstract":"<div><h3>Background</h3><div>Currently, there are no specific treatments for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), influenza and respiratory syncytial virus (RSV) infection. While phytochemicals have been explored for preventing viral entry, few approaches simultaneously target viral binding and enhance mucosal innate immunity. This study aimed to develop an oro-nasal film spray containing andrographolide and evaluate its antiviral, mucosal immune-enhancing, and anti-inflammatory effects.</div></div><div><h3>Methods</h3><div>The antiviral activity of andrographolide and the formulated spray was assessed using a SARS-CoV-2 spike pseudovirus assay in HEK293-hACE2 cells expressing TMPRSS2. Plaque reduction assays were conducted against SARS-CoV-2, influenza A/H3N2, A/H1N1, and RSV. ELISA was used to measure mucosal immune markers (LL-37 and HD-5), while inflammatory cytokine levels were analyzed via multiplex bead immunoassay. Nitric oxide inhibition was used to assess anti-inflammatory activity.</div></div><div><h3>Results</h3><div>The andrographolide spray (10 µg/ml) inhibited SARS-CoV-2 pseudovirus infection with ∼80% cell viability. It showed strong plaque reduction against SARS-CoV-2, partial inhibition of influenza A/H3N2, and no effect on RSV or A/H1N1. The spray significantly induced LL-37 and HD-5 expression in oral keratinocytes at 24 and 48 h and demonstrated dose-dependent anti-inflammatory effects.</div></div><div><h3>Conclusion</h3><div>The andrographolide film spray exhibits dual functionality—antiviral activity against SARS-CoV-2 and influenza A/H3N2, and enhancement of mucosal innate immunity <em>in vitro</em>. These findings support its potential as a preventative strategy at the primary site of viral entry. Further <em>in vivo</em> investigation is warranted to explore and confirm its effects.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100957"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis, biological activities, and network pharmacology of Sterculia hamiltonii leaves, barks, and roots ethanol extract","authors":"Ananta Kumar Das ,&nbsp;Pria Khan Prianka ,&nbsp;Israt Jahan ,&nbsp;Mitu Akter ,&nbsp;Md.Sadnan Sakib ,&nbsp;Sonia Akter ,&nbsp;Joy Saha ,&nbsp;Uma Rani Sarkar ,&nbsp;Prosenjit Paul ,&nbsp;Md.Habibur Rahman ,&nbsp;Nazmul Hossain ,&nbsp;Mst. Marzia Khatun ,&nbsp;Utpaul Chandra Das ,&nbsp;Koushik Saha","doi":"10.1016/j.phyplu.2025.100951","DOIUrl":"10.1016/j.phyplu.2025.100951","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to analyze phytochemicals, biological activities, and therapeutic mechanisms of <em>Sterculia hamiltonii</em> leaf, bark, and root ethanol extracts.</div></div><div><h3>Methods</h3><div>This study extracts <em>S. hemiltonii</em> leaf, bark, and root with ethanol and names them SHEL, SHEB, and SHER. Phytochemical screening identifies bioactive compounds, while GC-MS analysis is for chemical profiling. <em>In-vitro</em> and <em>in-vivo</em> bioassays for antioxidant, cytotoxicity, analgesic, and anti-inflammatory activity, network pharmacology investigates molecular mechanisms and pathways linking bioactive molecules.</div></div><div><h3>Results and discussions</h3><div>The phytochemical analysis found alkaloids, glycosides, saponins, flavonoids, and phenolics in all extracts. The barks had more total phenolics. Total flavonoid data showed leaf extract had a non-significantly higher content. Barks and roots have more total antioxidant and ferric reduction capacity than leaves. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging test, the SHEB and SHER showed strong antioxidant activity with IC₅₀ values of 27.26 and 26.95 µg/mL. In the brine shrimp cytotoxicity investigation, SHEL showed significant activity. Analgesic studies showed leaves, barks, and root extracts have significant effects in the hot plate, with maximum activity at 60 min. 69.59% in 500mg/kg, and acetic acid induced the highest writhing inhibition was 71% in 250mg/kg. In xylene-induced ear edema, leaf and root extracts had better anti-inflammatory action. The GC–MS study of <em>S. hemiltonii</em> leaf, bark, and root extracts found 76, 67, and 73 phytochemicals, respectively. Literature studies of the constituents showed that many compounds have biological uses like antioxidant, antimicrobial, analgesic, anti-inflammatory, and anticancer properties. Network pharmacology of phytochemicals may treat Alzheimer’s, cancers, epilepsy, hypertension, diabetes, etc.</div></div><div><h3>Conclusions</h3><div>The studies of <em>S. hemiltonii</em> extracts revealed the presence of many potential phytochemicals, which are responsible for antimicrobial, anti-inflammatory, anticancer, etc. effects. <em>In-vitro, in-vivo</em>, and <em>in-silico</em> studies also prove the significance of the <em>effects of S. hemiltonii extract</em>.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100951"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals and novel delivery systems in alopecia management: An integrative review","authors":"Kampanart Huanbutta ,&nbsp;Napapat Rattanachitthawat ,&nbsp;Anusorn Thampithak ,&nbsp;Phatsakorn Onnim ,&nbsp;Inderbir Singh ,&nbsp;Tanikan Sangnim","doi":"10.1016/j.phyplu.2025.100949","DOIUrl":"10.1016/j.phyplu.2025.100949","url":null,"abstract":"<div><div>Alopecia, a common dermatological disorder characterized by hair loss, can significantly impact psychological well-being and self-esteem despite being non-life-threatening. Various factors, including genetics, hormonal imbalances, environmental stressors, and medical conditions, contribute to its development. Conventional treatments such as minoxidil and finasteride stimulate hair growth by prolonging the anagen phase or inhibiting dihydrotestosterone production but may cause adverse effects, prompting interest in alternative therapies. Recent studies have highlighted the potential of herbal medicines and phytochemicals such as epigallocatechin gallate from green tea, curcumin from turmeric, and essential oils like rosemary and peppermint for their anti-inflammatory, antioxidant, and hormonal-modulating properties. A combination of synthetic and natural therapies offers a promising synergistic approach, enhancing efficacy while reducing side effects. However, conventional topical formulations often face limitations in skin penetration and bioavailability. To address this, innovative transdermal drug delivery systems, including nanocarriers, liposomes, and microneedles, have been explored to enhance follicular targeting and improve therapeutic outcomes. This review summarizes recent advances in alopecia management, covering synthetic and herbal therapies, mechanisms of action, novel delivery strategies, evaluation methods, and commercial products. By integrating traditional remedies with modern technologies, future treatments may offer improved therapeutic outcomes and greater patient satisfaction.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100949"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A decade of beetroot (Beta vulgaris) research 2015–2025: Phytoconstituents, mechanisms, preclinical and clinical insights","authors":"Sarder Arifuzzaman ,&nbsp;Md. Harun-Or-Rashid ,&nbsp;Rahima Akter ,&nbsp;Farjana Akter Rupon ,&nbsp;Md. Shakil ,&nbsp;Mst Reshma Khatun","doi":"10.1016/j.phyplu.2025.100946","DOIUrl":"10.1016/j.phyplu.2025.100946","url":null,"abstract":"<div><h3>Background</h3><div>Over recent decades, a significant body of literature has emerged, encompassing phytochemical profiling, mechanisms of action, in-vitro studies, animal and Clinical trials, collectively building a compelling case for beetroot’s therapeutic potential. However, molecular pharmacological activities, bioavailability and long-term clinical validation remain crucial for translating beetroot’s potential into evidence-based therapeutic applications.</div></div><div><h3>Aim of the Study</h3><div>This review aims to provide an overview of the current literature, highlight gaps in knowledge, and offer suggestions for future research directions on the pharmacological activities of beetroot and synthesis its bioactive constituents—such as betacyanins, nitrates, polyphenols and flavonoids—through both mechanistic and clinical evidence.</div></div><div><h3>Methods</h3><div>More than 200 studies were reviewed from multiple databases such as PubMed, Google Scholar, ScienceDirect®, ClinicalTrials.gov and Scopus. Bioinformatics and cheminformatics tools were utilized to synthesize and visualize relevant findings and figures.</div></div><div><h3>Results</h3><div>Beetroot is rich in bioactive compounds, including betacyanin and betaxanthin, nitrates, phenolic and flavonoid compounds, saponins and triterpenoids, fatty acids and amino acids. Reported molecular mechanism experiments suggest that beetroot exerts biological effects by targeting multifactorial pathways (e.g., HMG-CoA, Beta-glucosidase, COX-2, among others). In vitro studies demonstrated antiproliferative and pro-apoptotic effects on cancer cells, while in vivo studies confirmed its metabolic, cardiovascular and hepatoprotective benefits. Clinical trials supported improvements in blood pressure, glycemic control and exercise performance in healthy individuals, hypertensive patients, those with diabetes, and even COVID-19 patients.</div></div><div><h3>Conclusion</h3><div>Overall, our review insights will be essential for developing beetroot-based therapeutic applications and guiding for future research.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100946"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic and experimental identification of TCMs enhancing miR-708 expression in triple-negative breast cancer","authors":"Nai-Jung Cheng ,&nbsp;Zhong-Liang Wang ,&nbsp;Yi-Chen Huang ,&nbsp;Yuan-Chieh Yeh ,&nbsp;Kai-Ti Lin","doi":"10.1016/j.phyplu.2025.100944","DOIUrl":"10.1016/j.phyplu.2025.100944","url":null,"abstract":"<div><h3>Background</h3><div>Breast cancer remains the most prevalent and life-threatening malignancy among women, with triple-negative breast cancer (TNBC) posing a major clinical challenge due to the lack of targeted therapies. Traditional Chinese medicine (TCM), a comprehensive system of knowledge refined over thousands of years, has gained increasing recognition for its potential in cancer treatment, including TNBC management.</div></div><div><h3>Purpose</h3><div>Our previous research identified miR-708 as a promising therapeutic target. This study aimed to apply bioinformatic screening to identify potential TCMs that regulate miR-708 expression and inhibit TNBC progression, offering potential for integrating traditional medicine into modern cancer treatment.</div></div><div><h3>Result</h3><div>By using bioinformatic screening with experimental validation, we identified and confirmed that <em>Astragalus membranaceus, Sophora flavescens, Bupleurum chinense</em>, and <em>Glycyrrhiza uralensis</em> effectively induced miR-708 expression and reduced NRAS and Rap1B expression, leading to the inhibited proliferation, anchorage-independent growth ability and induced apoptosis in MDA-MB-231 and HCC1395 TNBC cell lines. Among them, <em>Sophora flavescens</em> and <em>Glycyrrhiza uralensis</em> showed the strongest effects, with <em>Glycyrrhiza uralensis</em> also significantly suppressing cell migration.</div></div><div><h3>Conclusion</h3><div>Taken together, our findings suggest that <em>Glycyrrhiza uralensis</em> may serve as a promising candidate for TNBC treatment, potentially paving the way for novel therapeutic strategies.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100944"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential influence of Oroxylum indicum root and leaf metabolites on the proliferation and migration of breast cancer cells","authors":"Advait Agashe ,&nbsp;Supriya K. Acharya ,&nbsp;Vedangi Hyatnagarkar ,&nbsp;Javed Mulla ,&nbsp;Vitthal T. Barvkar ,&nbsp;Tuli Dey ,&nbsp;Vaijayanti Tamhane","doi":"10.1016/j.phyplu.2026.100958","DOIUrl":"10.1016/j.phyplu.2026.100958","url":null,"abstract":"<div><h3>Background</h3><div>Exploring the tissue-wise (leaf vs. root) therapeutic potential of medicinally important plants like <em>Oroxylum indicum</em> (L.) Kurz widens the scope to decipher newer treatment regimens for diseases like cancer.</div></div><div><h3>Purpose</h3><div>We attempted to gain insight in this mechanism of differential anti-cancer potential of leaf and root extracts of the tree <em>O. indicum</em>.</div></div><div><h3>Methods</h3><div>LC-MS/MS analysis and cytotoxicity of the <em>O. indicum</em> leaf and root extracts was performed. In-solution proteomics of the extract-treated and control MCF-7 cancer cells deciphered several differential proteins. <em>In vitro</em> experiments like MTT assay, caspase activity assay, migration assay, and real- time quantitative PCR helped validate the findings.</div></div><div><h3>Results</h3><div>The <em>O. indicum</em> leaf and root extracts have a differential abundance of various bioactive flavonoids like apigenin and biochanin-A. Leaf and root extract treatment modulated &gt;2200 MCF-7 proteins belonging to several gene-ontology classes prominently representing nucleic-acid binding and cytoskeletal proteins; proteins regulating diverse biological and metabolic processes like signal transduction, enzyme modulation, membrane trafficking, protein folding, etc. More than 200 uniquely expressed proteins like DDX10, Septin-2, etc. were detected in <em>O. indicum</em> leaf and root extract-treated cells. The root-extract treated cells followed a strong, concentration-dependent apoptotic modality as reflected by their caspase activity, along with a differential regulation of genes regulating cell proliferation and apoptosis. Migration of the <em>O. indicum</em> root extract-treated cells was significantly reduced, as compared to that of the leaf extract-treated cells, reflecting likewise in the respective gene expression profiles.</div></div><div><h3>Conclusion and implications</h3><div>Metabolites from <em>O. indicum</em> root extracts cause proteome level changes in MCF-7 cells that manifest into potent migrastatic and anticancer properties which can be tapped for greater phytotherapeutic advances.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100958"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chang'an II Recipe alleviates visceral hypersensitivity in IBS-D rats by inhibiting mast cell activation through the AhR-Notch1 pathway","authors":"Xiao-ge Wang ,&nbsp;Min Zhang ,&nbsp;Ting Chen ,&nbsp;Jin-xin Ma ,&nbsp;Ze-lun Li ,&nbsp;Feng-yun Wang ,&nbsp;Xu-dong Tang ,&nbsp;Wen-xia Zhao","doi":"10.1016/j.phyplu.2025.100930","DOIUrl":"10.1016/j.phyplu.2025.100930","url":null,"abstract":"<div><h3>Background</h3><div>Irritable bowel syndrome with diarrhea (IBS-D) is a common functional gastrointestinal disorder. It is often accompanied by anxiety and depression, and severely impacts patients' quality of life. Visceral hypersensitivity is closely associated with the pathogenesis of IBS-D, potentially involving activation of the AhR-Notch1 signaling pathway in mast cells. Chang'an II recipe is a formulation developed by Prof. Tang Xudong based on the classical formula of Tong-Xie-Yao-Fang, which has documented clinical efficacy in the treatment of IBS-D. However, the mechanism underlying the action of the Chang'an II recipe in treating IBS-D is not fully understood.</div></div><div><h3>Objective</h3><div>To investigate the ameliorative efficacy of the Chang'an II recipe on rat models of IBS-D, as well as its underlying mechanism in IBS-D rats and the RBL-2H3 rat basophilic leukemia cell line.</div></div><div><h3>Methods</h3><div>The IBS-D model rats received high, medium, and low doses of the Chang'an Ⅱ recipe and the AhR inhibitor Kyn by oral gavage for 14 days. The body weight, food intake, fecal water content (FWC), abdominal withdrawal reflex (AWR), and severity of diarrhea symptoms were assessed. Sera and colonic tissues were collected for histopathological analyses, and relevant indices were measured in tissues and RBL-2H3 cells.</div></div><div><h3>Results</h3><div>The Chang'an II recipe effectively alleviated weight loss and increased food intake and intestinal absorption in IBS-D rats. It also alleviated diarrhea, decreased visceral hypersensitivity, and reduced intestinal inflammation. The mechanism underlying the reduction in visceral hypersensitivity was associated with AhR upregulation and Notch1 downregulation, reducing activation of p-p38, p-JNK, and p-ERK1/2. This process suppressed mast cell activation and inhibited the production of biologically active substances such as mast cell tryptase, histamine, IL-4, and TNF-α.</div></div><div><h3>Conclusion</h3><div>The Chang’an II recipe alleviates IBS-D symptoms by modulating the AhR/Notch1 signaling pathway, suppressing mast cell activation, and downregulating the PAR2/TRPV1 axis in intestinal afferent nerves. These findings provide novel mechanistic insights into its therapeutic effects, highlighting its potential as a targeted treatment for IBS-D.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100930"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the therapeutic promise of Rosa canina L.: A phytochemical and ethnopharmacological approach","authors":"Apporva Chawla ,&nbsp;Amit Kumar ,&nbsp;Ankur Kumar Tanwar ,&nbsp;Jasreen Uppal ,&nbsp;Sukesh Kumar Gupta ,&nbsp;Vikash Prashar ,&nbsp;Prince Ahad Mir ,&nbsp;Nishant Kumar","doi":"10.1016/j.phyplu.2025.100947","DOIUrl":"10.1016/j.phyplu.2025.100947","url":null,"abstract":"<div><div><em>Rosa canina</em> L., commonly known as “rosehip” or “dog rose,” belongs to the family Rosaceae and is recognized as a rich reservoir of polyphenolic compounds. Traditionally valued for its therapeutic and health-promoting properties, it has been employed not only in the treatment of dog bites but also as an antirheumatic, astringent, ophthalmic, and vermifuge agent. The berries, noted for their high vitamin C content, are frequently consumed as tea believed to enhance vitality and alleviate various ailments. Data pertaining to its phytochemistry, pharmacological properties, biosynthesis of secondary metabolites, and toxicological evaluations were retrieved from scientific databases including Elsevier, PubMed, Sci-Finder, Web of Science, and Google Scholar. Botanical nomenclature was verified through The Plant List and World Flora Online (accessed August 8, 2024). This review comprehensively examines the ethnomedicinal relevance, chemical composition, traditional applications, and experimentally validated pharmacological effects of <em>R. canina</em>. The major phytoconstituents identified in this species include flavonoids, phenolic acids, carotenoids, galactolipids, tannins, and various organic and inorganic acids. Pharmacological investigations have demonstrated its anticancer, antioxidant, anti-inflammatory, hepatoprotective, antihyperglycemic, antiarthritic, and analgesic potential in both in vitro and <em>in vivo</em> models. Clinical studies available in the literature are also discussed. Overall, the present review provides an integrative and updated account of the ethnobotanical significance, bioactive constituents, and therapeutic potential of <em>Rosa canina</em> L.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"6 1","pages":"Article 100947"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}