Phytomedicine Plus最新文献

{"title":"Advancing global research on Datura stramonium L.: Integrative in vitro antifungal, antioxidant, and in silico molecular docking studies","authors":"Ankit kale ,&nbsp;Deepshikha Patle ,&nbsp;Shyam Ingle ,&nbsp;Durgesh M. Agase","doi":"10.1016/j.phyplu.2025.100763","DOIUrl":"10.1016/j.phyplu.2025.100763","url":null,"abstract":"<div><h3>Background</h3><div>Plant-based drugs are vital in modern medicine due to their effectiveness, lower side effects, and potential in treating chronic diseases and infections. <em>Datura stramonium</em> L.<em>,</em> a plant long recognized for its therapeutic properties, was investigated in this study to further explore its potential antioxidant and antifungal effects.</div></div><div><h3>Methodology</h3><div>The study aims to optimize ethanolic extracts from <em>D. stramonium</em> L. leaves to evaluate their antifungal and antioxidant properties using Disc diffusion method and DPPH free radical scavenging assay. Additionally, the bioactive compounds within the extracts were identified through GC–MS analysis and subsequently screened using molecular docking studies by docking the identified constituents with selected proteins of fungus <em>candida albicans.</em></div></div><div><h3>Result</h3><div>The antifungal activity of the extract was tested against the fungus <em>C. albicans ATCC10231</em> strain using a disc diffusion assay, with fluconazole serving as the positive control for determining the MIC. The extract exhibited maximum potency at 250 µg/mL and an MIC of 50 µg/mL. In the DPPH scavenging assay, the ethanolic leaf extract of <em>D. stramonium</em> L. demonstrated an IC₅₀ value of 146.69 ± 8.46 μg/mL. GC–MS analysis identified two major constituents: Heneicosane and Heptadecane, 2,6,10,15-tetramethyl. These compounds were further evaluated via molecular docking studies. Docking simulations with mannose-binding lectin proteins (1IYL, 1AI9) and aspartic protease proteins (1ZAP, 2H6S) of <em>C. albicans</em> showed that both phytoconstituents effectively bound to the target proteins, with the highest binding affinity observed for the 1ZAP protein.</div></div><div><h3>Conclusion</h3><div>The findings of this study demonstrate the significant antifungal activity of <em>D. stramonium</em> L. against the <em>ATCC10231</em> strain of <em>C. albicans</em>, along with notable antioxidant effects, likely attributed to the identified phytoconstituents. These results suggest a promising potential for synthesizing derivatives to enhance the efficacy of these bioactive compounds.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100763"},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of analgesic and anthelmintic activities of Artocarpus chaplasha ROXB. leaves supported by in silico molecular docking","authors":"S M Sohag ,&nbsp;Sharmin Nur Toma ,&nbsp;Md. Niaj Morshed ,&nbsp;Md. Al Imran Imon ,&nbsp;Md. Monirul Islam ,&nbsp;Md. Ibnul Piash ,&nbsp;Naznin Shahria ,&nbsp;Imran Mahmud","doi":"10.1016/j.phyplu.2025.100761","DOIUrl":"10.1016/j.phyplu.2025.100761","url":null,"abstract":"<div><div><em>Aartocarpus chaplasha</em> is a medicinal tree native to tropical regions, valued for its diverse therapeutic properties and bioactive compounds found in its leaves, bark, and fruit. This study investigates the analgesic and anthelmintic properties of the ethanolic leaf extract of <em>Aartocarpus chaplasha</em> (ELAC). HPLC analysis identified key compounds in ELAC, including catechin hydrate, epicatechin, rutin hydrate, p-coumaric acid, rosmarinic acid, quercetin, and kaemferol. ADMET properties and molecular docking analysis were performed using the identified compounds and analgesic targets COX-1 and COX-2 and the anthelmintic target tubulin-colchicine. Analgesic activity was assessed using the acetic acid-induced writhing test on Swiss albino mice, while anthelmintic effects were evaluated against <em>Paramphistomum cervi. In silico</em> molecular docking suggested that catechin hydrate and kaempferol could be promising alternatives for analgesic activity due to their higher binding energy with COX-1 and COX-2. Moreover, rutin hydrate showed strong binding energy with tubulin-colchicine against anthelmintic properties. At a 500 mg/kg dosage, ELAC exhibited significant analgesic effects. In the anthelmintic tests, a 100 mg/mL concentration resulted in the lowest paralysis and death times of 14.03 and 18.03 mins, respectively. Our present study suggests that <em>Aartocarpus chaplasha</em> leaves can be a potential source of lead compounds for pain management and helminth infections and further advanced investigations are required in the future.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100761"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of Red and White Ginger (Zingiber officinale) Rhizomes and Gingerol improves male reproductive capacities","authors":"Monday Obianke Ebeye ,&nbsp;Bartholomew Chukwuebuka Nwogueze ,&nbsp;Obidike Alexender Naiho ,&nbsp;John Chukwuka Igweh","doi":"10.1016/j.phyplu.2025.100758","DOIUrl":"10.1016/j.phyplu.2025.100758","url":null,"abstract":"<div><h3>Introduction</h3><div>The use of medicinal plants in herbal medicine is increasing steadily, and the beneficial medical effects of plant materials have recently been the subject of many scientific research. These effects generally result from the combination of secondary products present in the plants. <em>Zingiber officinale,</em> one of the many herbs with great potential, was used in this study. The purpose of this study was to determine the effect of red and white ginger (<em>Z. officinale</em>) rhizomes and 6 gingerol on the reproductive capacities of male Wistar rats.</div></div><div><h3>Materials and methods</h3><div>Seventy male rats were purchased and used in the study. The animals were randomly distributed into ten groups of seven rats, each group depending on the nature of the treatment with ginger rhizome. All animals were fed with normal rat chow and water. All the experimental rats were treated for four weeks. At the end of the treatment period, the experiment rats were humanely euthanized and semen was collected for sperm count, motility, viability, epididymal volume and morphology, while plasma from whole blood was collected for hormonal assays.</div></div><div><h3>Results</h3><div>Findings from our study showed that red and white ginger <em>Z. officinale</em> rhizomes and gingerol compound caused significant (<em>p</em> &lt; 0.05) increase in the body weights of rats compared to the control group. Similarly, there was a significant (<em>p</em> &lt; 0.05) increase in the relative testicular and epididymal organ weights, as well as a significant (<em>p</em> &lt; 0.05) increase in the sperm count, motility, viability, and epididymal volume with a corresponding reduction in abnormal sperm morphology was observed. Administration of red and white <em>Z. officinale</em> rhizomes and gingerol produced significant (<em>p</em> &lt; 0.05) increase in the levels of Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and testosterone, respectively. In addition, there was a significant (<em>p</em> &lt; 0.05) increase in the testosterone/LH ratio in experimental rats compared to the control group.</div></div><div><h3>Conclusion</h3><div>Bioactive compound derivatives of <em>Z. officinale</em> rhizomes (Red and white ginger) and 6 gingerol acts as a potent fertility promoters and are apparently safe for used in pregnancy, enhancing male reproductive success.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100758"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer potential of Syzygium cumini and Syzygium Jambolanum extracts against MCF-7 cell line: An in vitro evaluation","authors":"Sheikh Muhammad Suhail ,&nbsp;Amit Anand ,&nbsp;Abu Safana Biswas ,&nbsp;Santhepete Nanjundiah Manjula ,&nbsp;Kenganora Mruthunjaya","doi":"10.1016/j.phyplu.2025.100753","DOIUrl":"10.1016/j.phyplu.2025.100753","url":null,"abstract":"<div><h3>Background</h3><div>This research has been carried out to evaluate the anticancer activity of hydroalcoholic extracts of <em>Syzygium cumini</em> and <em>Syzygium jambolanum</em> against human MCF-7 cells .</div></div><div><h3>Purpose</h3><div>This study aims to identify the bioactive compounds present in the extracts, assess their <em>in vitro</em> antioxidant and anticancer potential, along with the possible mechanism of action for its anticancer property.</div></div><div><h3>Methods</h3><div>Hydroalcoholic extracts of <em>S. cumini</em> and <em>S. jambolanum</em> fruit pulp, Juice, Seed was subjected to GC–MS to identify its chemical compositions followed by invitro antioxidant cytotoxicity study by DPPH, MTT assay respectively. Further the promising extract was tested for Flow cytometric analysis. Apoptosis and qRT-PCR studies to investigate the mechanism of anticancer. Results were analyzed with GraphPad Prism 8 software and calculated as mean±SEM, p-value of &lt;0.05 was considered to be statistically significant.</div></div><div><h3>Results</h3><div>GC–MS analysis of hydroalcoholic extracts of <em>Syzygium cumini</em> and <em>Syzygium Jambolanum</em> (Fruit Pulp, Seed, Juice) found 113 compounds &amp; indicated significant amounts of n-hexadecanoic, tridecanoic, and dodecanoic acids. Among the extract <em>S. cumini</em> seed extract showed considerable antioxidant activity and the IC₅₀ value 45 μg/ml though both <em>S.C</em> &amp; <em>S.</em>J H.C extracts of Fruit pulp, Juice had and marked Cytotoxicity activity against MCF-7 cells with IC₅₀ value 80.75 μg/ml. Further mechanistic studies revealed that both the <em>S.C &amp; S.</em>J seed extracts showed cell cycle arrest in S phase and caused induction of apoptosis, via the increased, of p53 by western blotting studies.</div></div><div><h3>Conclusion</h3><div>The findings suggest that among hydroalcoholic extracts of <em>S. cumini</em> and <em>S. jambolanum.</em> S. cumini possess potential anticancer properties. Further studies can be warranted to explain the mechanisms of action and explore their therapeutic applications.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100753"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the influence of matcha green tea extract on NF-κB/MAPK signaling pathway activation in rats: A study on irradiation-induced acute hepatic inflammatory response","authors":"Noha Sayed Hamed,&nbsp;Eithar K. El Adham,&nbsp;Amal I. Hassan","doi":"10.1016/j.phyplu.2025.100730","DOIUrl":"10.1016/j.phyplu.2025.100730","url":null,"abstract":"<div><h3>Background</h3><div>Numerous forms of cancer are frequently treated with radiation therapy, which provides substantial benefits in tumor control. However, adverse effects, particularly the development of severe hepatitis, may render radiation therapy ineffective.</div></div><div><h3>Purpose</h3><div>This study investigates the potential of matcha (M) green tea extract, a natural compound known for its high antioxidant properties and health-promoting properties, in a new approach to reduce radiation-induced liver damage.</div></div><div><h3>Study design and method</h3><div>In this experimental investigation, the administration of M was conducted on female Wistar rats for duration of two weeks after gamma-ray irradiation. Next, a series of biochemical experiments were conducted to examine the impact of M on lipid peroxidation levels, antioxidant enzyme activities, and pro-inflammatory cytokines (IL-6, IL-10, and TNF-α) in liver tissue. The Western blot method was employed for this analysis. Liver miR-122 expression levels were determined using real-time reverse transcription polymerase chain reaction. In addition, the present study investigated the mechanism by which M stops the inflammatory process's crucial NF-κB/MAPK signaling.</div></div><div><h3>Results</h3><div>This study suggests that the NF-κB/MAPK signaling pathway that triggers the radiation-induced inflammatory response is further inhibited by M making radiation therapy effective and safe.</div></div><div><h3>Conclusion</h3><div>Further research in this area may reveal the underlying mechanisms and pave the way for the development of novel therapies for radiation therapy patients.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100730"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antimicrobial and antioxidative potential of Leucas aspera (Willd.) link: Phytochemical screening, molecular docking, and HR-LC/MS profiling against SARS-CoV-2 protein 3CLPro, Spike and RDB","authors":"Thavasiaanatham Seenivasan Shalini ,&nbsp;Ragothaman Prathiviraj ,&nbsp;Poomalai Senthilraja","doi":"10.1016/j.phyplu.2024.100700","DOIUrl":"10.1016/j.phyplu.2024.100700","url":null,"abstract":"<div><h3>Background</h3><div><em>Leucas aspera</em> (Willd.) Link, a member of the Lamiaceae family and commonly known as \"Thumbai,\" is found throughout India and has been utilized in traditional Indian medicine to treat various ailments.</div></div><div><h3>Purpose</h3><div>This study entails methanolic extraction of <em>Leucas aspera</em> (Willd.) Link using a Soxhlet apparatus, followed by phytochemical screening and antioxidant activity assessment.</div></div><div><h3>Methods</h3><div>The <em>L. aspera</em> methanolic leaf extract displayed a phenolic content of 100 µg/ml, consisting of 3.02 mg of gallic acid dry weight equivalents and a flavonoid content of 100 µg/ml with 3.35 mg quercetin equivalents per dry weight. The extract's free radical scavenging capabilities were at 150 µg/ml in DPPH, showing an 87 % effectiveness compared to the standard ascorbic acid 49 % inhibition capacity. The ABTS radical scavenging activity in 100 µg/ml extract measured 64.32 %, phosphomolybdate assay indicated 86.89%, and hydroxide radical scavenging capacity showed 126.72 %. Antibacterial activity was also assessed through agar well diffusion assays against <em>Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus</em>, and <em>Leucobacter sp</em>., Where <em>K. pneumoniae</em> exhibits the highest zone of inhibition. The methanolic crude extract of <em>L. aspera</em> was further using TLC, FT-IR and HR-LC/MS to identify functional groups and phytocompound present in the extract.</div></div><div><h3>Results</h3><div>HRLC-MS was used to conduct metabolite profiling of the methanolic crude extract of <em>L. aspera</em> leaves, discovering 37 positive and 43 negative compounds. To evaluate the ADMET properties and predict the drug-likeness of these 80 phytochemicals, an <em>in silico</em> analysis was performed. The results of this analysis revealed that only 19 of the compounds met the ADMET limitations and had suitable Log P values. Molecular docking of the 19 compounds against the SARS-CoV-2 main protease (3CL^Pro) protein (PDB ID: 6LU7) and spike protein receptor binding domain (SGp-RBD) protein (PDB ID: 2GHV) revealed Famprofazone and Loxtidine compounds having the highest binding affinity with LibDock scores of 105.53 and 116.70 respectively.</div></div><div><h3>Conclusion</h3><div>Thus, our findings could serve as potential active molecules of <em>L. aspera</em> against this target protein. This study provides further proof of bioactive compounds produced by the <em>L. aspera</em> leaf extract.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100700"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical composition and antioxidant properties of Canarium odontophyllum (Sarawak Olive): A seasonal fruit from Borneo, East Malaysia","authors":"Gilbert Ringgit ,&nbsp;Bo Eng Cheong ,&nbsp;Muhammad Dawood Shah ,&nbsp;Shafiquzzaman Siddiquee","doi":"10.1016/j.phyplu.2024.100703","DOIUrl":"10.1016/j.phyplu.2024.100703","url":null,"abstract":"<div><h3>Background</h3><div>Canarium species are reported rich of polyphenol, alkaloids, flavones, and saponins compounds. Canarium odontophyllum from Borneo's variety, on the other hand is remaining unclear especially on the antioxidant activity towards radicals.</div></div><div><h3>Purpose</h3><div>To investigate phytochemical composition and antioxidant properties of C. odontophyllum.</div></div><div><h3>Methods</h3><div>Antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) assay, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, and ferric-reducing antioxidant power (FRAP) assay with different polarity of extraction solvents (methanol, acetone and hexane). The phytochemical composition was determined by Liquid Chromatography Mass Spectrometry Quadrupole Time of Flight (LCMS-QTOF).</div></div><div><h3>Results</h3><div>Acetone showed the highest scavenging activity in the DPPH (0.0307 mg/ml) and FRAP (3.926 mg/ml) assays, while hexane exhibited the highest activity in the ABTS assay (0.119 mg/ml). The LCMS-QTOF chromatogram identified 22 potential compounds. Among these, the major compounds oleamide (10.49 %) and emmotin A (7.23 %) have previously been reported to exhibit antioxidant activity. Other health-beneficial compounds were also found, such as palmitic amide (2.46 %), deoxymiroestrol (6.23 %), and N-Cyclohexanecarbonylpentadecylamine (48.92 %), which are important in preventing metal corrosion, regulating hormones, and distinguishing between N-palmitoylethanolamine-hydrolyzing acid amidase (NPAA) and fatty acid amide hydrolase (FAAH), respectively.</div></div><div><h3>Conclusion</h3><div>The results indicate that C. odontophyllum shows a promising potential an alternative drug in pharmaceutical developments.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100703"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the multifaceted roles of Sonneratia apetala and Nipa fruticans in coastal habitat restoration and bioactive properties discovery","authors":"Sabina Yasmine ,&nbsp;Rezwana Zaman Proma ,&nbsp;Md. Ripaj Uddin ,&nbsp;Mohammad Saydur Rahman ,&nbsp;Md. Hamedul Islam ,&nbsp;Muhammad Abdullah Al Mansur ,&nbsp;AHM Shofiul Islam Molla Jamal ,&nbsp;Abu Yousuf ,&nbsp;Md. Hasnain Mustak ,&nbsp;Sarker Kamruzzaman ,&nbsp;M. Mahboob Ali Siddiqi","doi":"10.1016/j.phyplu.2024.100687","DOIUrl":"10.1016/j.phyplu.2024.100687","url":null,"abstract":"<div><h3>Background</h3><div><em>Sonneratia apetala</em> and <em>Nipa fruticans</em> are mangrove species, perennial wild plants with significant therapeutic, ecological, and environmental value. They are extensively utilized by the local communities of the Sundarbans and coastal estuaries for various purposes. <em>Aim of the study:</em> This study aims to provide an in-depth review of these plants, relying on secondary data and information.</div></div><div><h3>Methods</h3><div>Compile the secondary data from at least 50 relevant pieces of literature.</div></div><div><h3>Results</h3><div><em>S. apetala</em> fruits exhibit substantial antioxidant, antimicrobial, analgesic, antidiarrheal, and anti-diabetic properties. Additionally, the bark and leaves offer considerable therapeutic benefits. The plant contains various phytochemicals such as polyphenols include flavonoids, anthocyanins and alkaloids along with carbohydrates, proteins, fiber, minerals (such as Ca, K, Mg, S, Cu, Fe, Zn, Mn), and vitamins (including thiamine and riboflavin). Its leaf and fruit extracts show potential for applications in the food industry and green chemistry. The seed extract demonstrates the highest cytotoxic properties (LC₅₀=36 μg/mL) among other parts of the plant, along with antibacterial effects and dose-dependent analgesic and antidiarrheal properties. The leaf and bark extracts exhibit significant antidiabetic potential, with IC₅₀ values of 0.286±0.022 and 0.432±0.01 mg/mL respectively. The seeds are particularly rich in polyphenols (300.1 mg GAE/g), flavonoids (30.6 mg CE/g), anthocyanins (2.3 μmol/g), and vitamin C (4.0 mg/g), showcasing their strong antioxidant properties. The pericarp, though lower in these compounds, still presents significant nutritional value, containing 55.4 mg GAE/g polyphenols and 14.6 mg CE/g flavonoids. Nutritional analysis shows the pericarp's moisture content is 84.2 %, along with high carbohydrates (29.6 %) and proteins (8.8 %), while the seed has 55.6 % moisture and 11.5 % proteins. <em>Nipa fruticans</em> presents lucrative findings, including vinegar production, low-fat biscuits with high nutrient values, bioethanol, among others. Various parts of this plant contain essential elements in significant amounts and only trace amounts of heavy metals, indicating its safety for medicinal purposes.</div></div><div><h3>Conclusion</h3><div>The phytochemical, nutritional, and elemental analyses of these plants highlight its potential health benefits, food value and significance in research. This comprehensive analysis underscores <em>S. apetala's</em> and <em>N. fruticans</em> broad nutritional and phytochemical significance, making it a valuable subject in studies of natural products and human health.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100687"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative proteomics and targeted metabolomics reveal the role of Cassia siamea L. extract in the response to high-fat diet-induced obesity in Drosophila","authors":"Ananya Dechakhamphu ,&nbsp;Nattapong Wongchum ,&nbsp;Theeraphan Chumroenphat ,&nbsp;Alongklod Tanomtong ,&nbsp;Sirithon Siriamornpun","doi":"10.1016/j.phyplu.2025.100743","DOIUrl":"10.1016/j.phyplu.2025.100743","url":null,"abstract":"<div><h3>Background</h3><div><em>Cassia siamea</em> L., an angiosperm native to Southeast Asia, has been traditionally used for various medicinal purposes, including antifever, antihypertensive, and antidiabetic applications. Despite its traditional use, limited scientific evidence supports its antiobesity properties.</div></div><div><h3>Purpose</h3><div>This study aimed to investigate the antiobesity effects of <em>C. siamea</em> extract (CSE) both <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Methods</h3><div>CSE was obtained via extraction from <em>C. siamea</em> wood with 70 % ethanol and its phytochemical content was analyzed by gas chromatography–mass spectrometry (GC‒MS) and high-performance liquid chromatography (HPLC). <em>Drosophila</em> was used to examine the effects of supplementation with <em>C. siamea</em> extract on lipase activity, body weight, triglyceride levels, physical strength, survival, and antioxidant activity in <em>Drosophila</em> fed a high-fat diet. Moreover, this study investigated the effects of the extract combined with a high-fat diet on amino acid metabolism and proteomic profiles to clarify its mechanism of action.</div></div><div><h3>Results</h3><div>Syringic acid was the most abundant phenolic compound in CSE, followed by gallic acid, sinapic acid, protocatechuic acid, cinnamic acid, chlorogenic acid, and ferulic acid. <em>In vitro</em>, CSE significantly inhibited pancreatic lipase activity (IC₅₀ = 67.73±3.24 µg/mL) in a noncompetitive manner. In a high-fat diet-fed <em>Drosophila</em> model, CSE supplementation (10 mg/mL) reduced lipase activity and triglyceride levels and improved body weight, suggesting antiobesity and potential antidiabetic effects. Additionally, CSE increased survival rates, physical strength, and antioxidant capacity, as evidenced by increased SOD1 activity and resistance to oxidative stress. CSE helped restore disrupted amino acid levels, particularly glutamine, alanine, and proline, indicating improved metabolic regulation. The results of the quantitative proteomics analysis indicate that a high-fat diet (HFD) alters proteins within metabolic pathways, which can be minimized in severity through CSE therapy.</div></div><div><h3>Conclusion</h3><div>These findings highlight the potential therapeutic role of CSE in managing obesity and related metabolic disorders.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100743"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143176459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of antioxidant and hypolipidemic activities of extracts of Citrus x aurantium leaves in vitro","authors":"Loulia Al Hawat,&nbsp;Loai Alallan","doi":"10.1016/j.phyplu.2024.100723","DOIUrl":"10.1016/j.phyplu.2024.100723","url":null,"abstract":"<div><h3>Objective</h3><div>This study was done to estimate the antioxidant and hypolipidemic activities of three extracts of <em>Citrus x aurantium</em> var. amara L. leaves, and to determine the total phenolic and flavonoids contents, as well as to find the relationship between leaves’ chemical (phenolic and flavonoids) content and their biological activities.</div></div><div><h3>Methods</h3><div>Three different extracts were prepared using distilled water, ethanol 70 %, and n-hexane. The Folin-Ciocalteu assay was used to determine the total phenolic content of extracts. The aluminum chloride assay was used to determine the total flavonoids content of extracts. The antioxidant activity of various concentrations of extracts was estimated by measurement of the ability of each extract to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. Hypolipidemic activity of various concentrations of extracts was estimated by measurement of the bile acid binding capacity.</div></div><div><h3>Results</h3><div>The water extract, which was obtained by decoction, showed the highest phenolic and flavonoids contents (268.948 ± 1.131 mg GAE/g DW and 50.676 ± 0.659 mg QE/g DW, respectively) followed by hydroalcoholic and hexanic extracts (243.732 ± 3.726, 215.870 ± 4.763 mg GAE/g DW, and 43.312 ± 1.506, 41.226 ± 0.967 mg QE/g DW, respectively; <em>p</em> &lt; 0.05). IC₅₀ values of DPPH scavenging activity were lower in water extract than hydroalcoholic and hexanic extracts (0.324 ± 0.025, 0.403 ± 0.009, and 0.414 ± 0.024 mg/mL, respectively; <em>p</em> &lt; 0.05). All extracts showed bile acid binding activity with IC₅₀ values of 0.347 ± 0.011, 0.388 ± 0.004, and 0.400 ± 0.011 mg/mL for water, hydroalcoholic, and hexanic extracts, respectively. There were high correlations between each of phenolic and flavonoid content and the biological activities of leaves’ extracts (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The water extract has the highest phenolic and flavonoids contents. <em>Citrus x aurantium</em> leaves’ extracts possess antioxidant and hypolipidemic activities <em>in vitro</em>. Properties observed indicate the usefulness of <em>Citrus x aurantium</em> leaves in managing hyperlipidemia and obesity worldwide.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 1","pages":"Article 100723"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143177032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}