白化病小鼠模型的增效抗肥胖作用研究

Q3 Pharmacology, Toxicology and Pharmaceutics

Phytomedicine Plus Pub Date : 2025-03-06 DOI:10.1016/j.phyplu.2025.100776

Pritimoni Das , Manas Das , Pranjan Barman , Naba Kumar Hazarika , Nabajyoti Goswami

{"title":"白化病小鼠模型的增效抗肥胖作用研究","authors":"Pritimoni Das , Manas Das , Pranjan Barman , Naba Kumar Hazarika , Nabajyoti Goswami","doi":"10.1016/j.phyplu.2025.100776","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This work aims at ameliorative evaluation of <em>in vivo</em> and <em>in silico</em> aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of <em>Phyllanthus urinaria</em> L. and <em>Adhatoda vasica nees</em> in High Fat Diet (HFD) induced Swiss albino mice.</div></div><div><h3>Methods</h3><div>Male Swiss albino mice (23–25 <em>g, n</em> = 6) fed with 60 % HFD were treated with a low dose (550 mg/kg b.wt.) and high dose (920 mg/kg b.wt.) of the PHF for 12 weeks. <em>In vivo</em> experiments were performed in subcutaneous adipose tissue and white adipose tissue in HFD induced obese Swiss Albino mice through serum lipid profiling, qPCR analysis of adipogenic, lipolytic genes as well as <em>in silico</em> studies for detection of potent anti-obesity compound through LCMS, molecular docking and simulation based molecular dynamics analysis of target protein CD36.</div></div><div><h3>Results</h3><div>The PHF-treated mice showed significant (<em>p</em> = 0.01) reduction of obesity by improving lipolysis and lowered adipogenesis than the non-treated obese group. The identified compound Granisetron showed higher binding energy as well as standard stability and notably higher interaction towards Granisetron in 100 ns with CD36 receptor than standard drug (∆G<sub>bind</sub> value -106.32 ± 2.51).</div></div><div><h3>Conclusion</h3><div>The study validated the efficacy of our novel PHF against HFD-induced obesity in a dose-related manner thereby establishing itself as a potential translational medicine, ensuring the utmost therapeutic value.</div></div>","PeriodicalId":34599,"journal":{"name":"Phytomedicine Plus","volume":"5 2","pages":"Article 100776"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model\",\"authors\":\"Pritimoni Das , Manas Das , Pranjan Barman , Naba Kumar Hazarika , Nabajyoti Goswami\",\"doi\":\"10.1016/j.phyplu.2025.100776\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>This work aims at ameliorative evaluation of <em>in vivo</em> and <em>in silico</em> aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of <em>Phyllanthus urinaria</em> L. and <em>Adhatoda vasica nees</em> in High Fat Diet (HFD) induced Swiss albino mice.</div></div><div><h3>Methods</h3><div>Male Swiss albino mice (23–25 <em>g, n</em> = 6) fed with 60 % HFD were treated with a low dose (550 mg/kg b.wt.) and high dose (920 mg/kg b.wt.) of the PHF for 12 weeks. <em>In vivo</em> experiments were performed in subcutaneous adipose tissue and white adipose tissue in HFD induced obese Swiss Albino mice through serum lipid profiling, qPCR analysis of adipogenic, lipolytic genes as well as <em>in silico</em> studies for detection of potent anti-obesity compound through LCMS, molecular docking and simulation based molecular dynamics analysis of target protein CD36.</div></div><div><h3>Results</h3><div>The PHF-treated mice showed significant (<em>p</em> = 0.01) reduction of obesity by improving lipolysis and lowered adipogenesis than the non-treated obese group. The identified compound Granisetron showed higher binding energy as well as standard stability and notably higher interaction towards Granisetron in 100 ns with CD36 receptor than standard drug (∆G<sub>bind</sub> value -106.32 ± 2.51).</div></div><div><h3>Conclusion</h3><div>The study validated the efficacy of our novel PHF against HFD-induced obesity in a dose-related manner thereby establishing itself as a potential translational medicine, ensuring the utmost therapeutic value.</div></div>\",\"PeriodicalId\":34599,\"journal\":{\"name\":\"Phytomedicine Plus\",\"volume\":\"5 2\",\"pages\":\"Article 100776\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytomedicine Plus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667031325000491\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytomedicine Plus","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667031325000491","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}

引用次数: 0

摘要

目的对高脂饮食（HFD）诱导的瑞士白化病小鼠，从体内和体内硅片方面对余甘子（Phyllanthus urinaria L.）和白菖蒲（Adhatoda vasica nees）多草药制剂（PHF）的水乙醇提取物进行改进评价。方法采用低剂量（550 mg/kg b.wt.）和高剂量（920 mg/kg b.wt.） PHF给药，连续12周，饲喂60% HFD的瑞士白化小鼠（23 ~ 25 g, n = 6）。在HFD诱导的肥胖瑞士白化小鼠的皮下脂肪组织和白色脂肪组织中进行了体内实验，通过血脂谱分析，qPCR分析脂肪生成和脂肪分解基因，并通过LCMS检测有效的抗肥胖化合物，分子对接和基于模拟的靶蛋白CD36分子动力学分析。结果与未治疗的肥胖组相比，phf组小鼠通过改善脂肪分解和降低脂肪生成而显著减少肥胖（p = 0.01）。所鉴定的复方格拉司琼与CD36受体在100ns内的相互作用（∆Gbind值-106.32±2.51）比标准药物具有更高的结合能和标准稳定性。结论本研究以剂量相关的方式验证了我们的新型PHF对hfd诱导的肥胖的疗效，从而确立了它作为潜在的转化医学，确保了最大的治疗价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A Synergistic anti-obesity effects of Phyllanthus urinaria and Adhatoda vasica nees formulation: An investigation in Swiss albino murine model

Objectives

This work aims at ameliorative evaluation of in vivo and in silico aspects of hydro-ethanolic extract of the Polyherbal Formulation (PHF) of Phyllanthus urinaria L. and Adhatoda vasica nees in High Fat Diet (HFD) induced Swiss albino mice.

Methods

Male Swiss albino mice (23–25 g, n = 6) fed with 60 % HFD were treated with a low dose (550 mg/kg b.wt.) and high dose (920 mg/kg b.wt.) of the PHF for 12 weeks. In vivo experiments were performed in subcutaneous adipose tissue and white adipose tissue in HFD induced obese Swiss Albino mice through serum lipid profiling, qPCR analysis of adipogenic, lipolytic genes as well as in silico studies for detection of potent anti-obesity compound through LCMS, molecular docking and simulation based molecular dynamics analysis of target protein CD36.

Results

The PHF-treated mice showed significant (p = 0.01) reduction of obesity by improving lipolysis and lowered adipogenesis than the non-treated obese group. The identified compound Granisetron showed higher binding energy as well as standard stability and notably higher interaction towards Granisetron in 100 ns with CD36 receptor than standard drug (∆G_bind value -106.32 ± 2.51).

Conclusion

The study validated the efficacy of our novel PHF against HFD-induced obesity in a dose-related manner thereby establishing itself as a potential translational medicine, ensuring the utmost therapeutic value.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊