2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第8页

Genetic algorithm based parameters adjustments for micron-order image analysis of metal fracture 基于遗传算法的金属断裂微米级图像分析参数调整

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351865

M. Mizoguchi, K. Obata, Y. Kato, K. Ogata

{"title":"Genetic algorithm based parameters adjustments for micron-order image analysis of metal fracture","authors":"M. Mizoguchi, K. Obata, Y. Kato, K. Ogata","doi":"10.1109/MHS.2009.5351865","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351865","url":null,"abstract":"A brittle fracture, which is a kind of metal fractures, accompanies little or no plastic deformations of the metal, The origin of the fracture is one of the most important clues to identify the cause of it, and can be located by observing the surface. Here, fractography is an area of study to analyze fracture mechanisms and/or causes by using electron microscope images. The method to locate the origins has been established through it. However, they have been done mainly by visual observations. At MHS2007, we proposed a method to improve its measurement accuracy to micron order by introducing computer vision techniques on Scanning Electron Microscope, or SEM, digital images [1]. However, some parameters were manually determined and required to be automated. This is a continued research of it. We propose, in this paper, a new method to determine proper parameters for image segmentation using Genetic Algorithms (GAs.) Through experiments, we proved the scheme worked properly, and suboptimal parameters were determined so that the greatest number of cleavage steps was obtained. It is expected that these proposed methods will enable non-experts to analyze metal fractures accurately in micron scale.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122440837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Miniaturization of a wide range load sensor using AT-cut quartz crystal resonator 采用at切割石英晶体谐振器的大量程负载传感器小型化

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351808

K. Narumi, T. Fukuda, F. Arai

引用次数: 2

Purification of wheat factors involving in translation initiation for reconstitution of protein synthesis 小麦蛋白合成重组翻译起始因子的纯化

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352036

H. Nagano, S. Fukada, H. Takagi, T. Ogasawara, Y. Endo, K. Takai

引用次数: 0

Giant liposome sorting/collection device: For individual analysis of artificial cell-models 巨型脂质体分选/收集装置:用于人工细胞模型的个体分析

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352048

S. Nomura, Li Liu, Yong Chen, H. Maruyama, F. Arai

引用次数: 1

Thermo-sensitive sol-gel transition and mechanical properties of poly(Depsipeptide-co-lactide)-g-PEG copolymers 聚(Depsipeptide-co-lactide)-g-PEG共聚物的热敏溶胶-凝胶过渡和力学性能

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351825

Y. Ohya, K. Nagahama, Y. Imai, Teppei Nakayama, T. Ouchi

引用次数: 0

Rotational speed control of Na+-driven flagellar motor by local ion concentration changes with multiple nano/micro pipettes 多个纳微移液管局部离子浓度变化对Na+驱动鞭毛马达转速的控制

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351830

K. Nogawa, M. Kojima, M. Nakajima, M. Homma, T. Fukuda

{"title":"Rotational speed control of Na+-driven flagellar motor by local ion concentration changes with multiple nano/micro pipettes","authors":"K. Nogawa, M. Kojima, M. Nakajima, M. Homma, T. Fukuda","doi":"10.1109/MHS.2009.5351830","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351830","url":null,"abstract":"Nano/micro robots have been actively studied toward the realization. Now, the micro living organisms are used as the driving forces for the micro objects. To achieve the fine control of bio driven micro objects, it is needed to establish the control method. Therefore, the local environmental control technique is desired to manipulate a single cell or to analyze the properties. We have constructed the local environmental control system with nano/micro dual pipettes, and achieved the quick-response and iterative rotational speed control of Na+-driven flagellar motor, which is a rotary molecular machine, by switching the local spout between Na+-containing and Na+-free solutions. In this paper, we upgrade our local environmental control system with nano/micro dual pipettes to achieve the quantitative and long-time-stable control of the rotational speed of the Na+-driven flagellar motor. We demonstrate fine and long-time-stable rotational speed control of Na+-driven flagellar motor by simultaneous local spouts of Na+-containing and Na+-free solutions with changing the spouting velocities independently.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126684144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discreet controller structure for robust oscillating nonlinear stable system 鲁棒振荡非线性稳定系统的离散控制器结构

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352017

M. Moteleb, Š. Kozák

引用次数: 0

Micro-encapsulation of bio-actuator using insect dorsal vessel 昆虫背血管微胶囊化生物致动器

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351970

Yuta Touyama, T. Hoshino, K. Iwabuchi, K. Morishima

引用次数: 5

Development of a pH indicator immobilized-gel-sheet for microenviroment analysis 微环境分析用pH指示剂固定化凝胶片的研制

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351885

T. Masuda, H. Maruyama, F. Arai, T. Anada, T. Fukuda, O. Suzuki

{"title":"Development of a pH indicator immobilized-gel-sheet for microenviroment analysis","authors":"T. Masuda, H. Maruyama, F. Arai, T. Anada, T. Fukuda, O. Suzuki","doi":"10.1109/MHS.2009.5351885","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351885","url":null,"abstract":"Detection of change in microenvironmental pH may be essential for analyzing biological processes, if transitory biodegradable scaffold materials are used for tissue engineering in bone regeneration. In this study, we created a pH indicator immobilized-gel-sheet for pH measurement. By using gel-sheet, we can investigate the local microenvironment of biodegradable materials, such as octacalcium phosphate (OCP), under the various protein adsorptions. The gel-sheet was made of a photocrosslinkable prepolymer solution (TUPR-5), which was easily immobilized by the method that the pH indicator is entrapped in a porous polymeric substrate. Bromothymol Blue (BTB) immobilized-gel-sheet was mounted in a biochip made of polydimethylsiloxane (PDMS) with a flow channel. The pH value was calculated by detecting the color changes, and presented as the pH distribution via a custom-made application. The pH sensing was carried out based on the surrounding color information of an OCP granule if incubated in culture medium. The gel-sheet detected the pH values of the condition around an OCP granule, showing the decrease from pH 7.8 to 8.8. As a whole, the proposed pH indicator is capable of providing pH level by high-spatial resolution.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131936757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Rapid immunosensing based on accumulation of microparticles by negative dielectrophoresis 基于负电介质电泳微粒子积累的快速免疫传感

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352000

T. Yasukawa, H. J. Lee, J. Ramón‐Azcón, Yusuke Yoshida, H. Shiku, T. Matsue, F. Mizutani

{"title":"Rapid immunosensing based on accumulation of microparticles by negative dielectrophoresis","authors":"T. Yasukawa, H. J. Lee, J. Ramón‐Azcón, Yusuke Yoshida, H. Shiku, T. Matsue, F. Mizutani","doi":"10.1109/MHS.2009.5352000","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352000","url":null,"abstract":"In the work, microfluidic device consisting of an interdigitated microarray (IDA) electrode was developed for a rapid, and separation-free immuno-sensors based on a manipulation technique of microparticles by dielectrophoresis (DEP). A poly-dimethylsiloxane (PDMS) substrate with microfluidic channel was placed on the IDA plate to allow to fabricating the device. On applying AC voltage to the IDA in a negative DEP (n-DEP) frequency region, goat anti-mouse immunoglobulin (anti-mouse IgG)-immobilized microparticles moved to the surface of PDMS substrate placed above the IDA by n-DEP force to accumulate at the designated areas of the PDMS surface, where anti-mouse IgG was precoated. When the fluorescence microparticles bearing anti-mouse IgG were suspended in an analyte (mouse IgG) solution, the microparticles trapped the analyte to form microparticle-conjugates. The conjugates were accumulated and captured at the designated areas of the PDMS surface via antibody-antigen-antibody (sandwich) reaction. The captured microparticles were detected selectively by fluorescence measurements at the focused, designated areas regardless of the presence of uncaptured microparticles in the suspended solution. Thus, the separation and washing-out steps, usually required for conventional immunoassay, are eliminated in the presented procedure. Since the formation of the sandwich structures was accelerated significantly by n-DEP, as short as 30 sec was enough to detect the immunoreaction at the surface. The fluorescence intensity of the captured microparticles at the designated area increased with the analyte in the range, 0.01 ~ 10 ng/mL. The present procedure realizes a rapid, sensitive and separation-free immunoassay in a simple device.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134074719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0