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Investigating Two-stage Tuberculin Skin Test for Screening Latent Tuberculosis Infections in Diabetic Patients: A Regional Study in Khorramabad, Iran 两阶段结核菌素皮肤试验筛查糖尿病患者潜伏结核感染的研究:伊朗霍拉马巴德的一项区域研究
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.75
Mahshad Mousavi, Nazanin Mousavi, Seyyed Amir Yasin Ahmadi, M. Kaviani
{"title":"Investigating Two-stage Tuberculin Skin Test for Screening Latent Tuberculosis Infections in Diabetic Patients: A Regional Study in Khorramabad, Iran","authors":"Mahshad Mousavi, Nazanin Mousavi, Seyyed Amir Yasin Ahmadi, M. Kaviani","doi":"10.52547/jommid.10.2.75","DOIUrl":"https://doi.org/10.52547/jommid.10.2.75","url":null,"abstract":"Investigating Two-stage Tuberculin Skin Test for Screening Latent Tuberculosis Infections in Diabetic Patients: A Regional Study in Khorramabad, Iran Mahshad Mousavi , Nazanin Mousavi , Seyyed Amir Yasin Ahmadi , Mozhgan Kaviani Student Research Committee, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Internal Medicine, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"267 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76744730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sequential HCoV-HKU1 and SARS-CoV-2 Infections, a Case Report HCoV-HKU1和SARS-CoV-2连续感染病例报告
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.93
A. Maleki, M. Pouriayevali, Mahsa Tavakoli, Zahra Ahmadi, Farideh Niknam Oskouei, Zahra Nejatipour, T. Jalali, Mostafa Salehi-Vaziri
{"title":"Sequential HCoV-HKU1 and SARS-CoV-2 Infections, a Case Report","authors":"A. Maleki, M. Pouriayevali, Mahsa Tavakoli, Zahra Ahmadi, Farideh Niknam Oskouei, Zahra Nejatipour, T. Jalali, Mostafa Salehi-Vaziri","doi":"10.52547/jommid.10.2.93","DOIUrl":"https://doi.org/10.52547/jommid.10.2.93","url":null,"abstract":"report a case in which the patient had sequential respiratory infections of human coronavirus HKU1 (HCoV-HKU1) and SARS-CoV-2 in a fully vaccinated, healthy person. It should be noted that other seasonal coronaviruses that could cause symptomatic RTIs might be misdiagnosed clinically with COVID-19. Hence, we highly recommend monitoring and follow-up of symptomatic patients with negative SARS-COV-2 RT-PCR results.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91050195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran 伊朗戈列斯坦省Gonbad-e Kavus临床标本中分离的肠杆菌科广谱β -内酰胺酶及其耐药表型
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.19
Mohammad-Hossein Akbari, A. Ahani Azari, L. Fozouni
{"title":"Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran","authors":"Mohammad-Hossein Akbari, A. Ahani Azari, L. Fozouni","doi":"10.52547/jommid.10.1.19","DOIUrl":"https://doi.org/10.52547/jommid.10.1.19","url":null,"abstract":"clinical specimens out of the samples. The isolates were Escherichia coli (36.1%), Klebsiella pneumonia (25%), Enterobacter cloacae (18.5%), Citrobacter freundii (11.1%) and Proteus mirabilis (9.2%). The highest resistance and susceptibility among the isolates belonged to sulfamethoxazole-trimethoprim (68.5%) and meropenem (11.1%), respectively. The highest prevalence of multidrug-resistant (MDR) and ESBL were observed in E. coli and Proteus mirabilis isolates . Conclusion: In this study, the high frequency of MDR phenotypes in the isolates may suggest an increasing trend of antibiotic resistance in Enterobacteriaceae . This could greatly impact the management and treatment of infections caused by these drug-resistant bacteria. Therefore, infection-control measures and continuous monitoring is recommended for controlling the spread of ESBL-producing strains in different geographical areas.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86069741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario 与免疫状态无关的结核分枝杆菌对非结核分枝杆菌的优势:印度情景
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.10
S. Dasgupta, Oindrila Chakraborty
{"title":"Mycobacterium tuberculosis Dominance over Nontuberculous Mycobacteria Irrespective of Immune Status: An Indian Scenario","authors":"S. Dasgupta, Oindrila Chakraborty","doi":"10.52547/jommid.10.1.10","DOIUrl":"https://doi.org/10.52547/jommid.10.1.10","url":null,"abstract":"Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India. Methods : Clinical samples from 112 suspected tuberculosis patients were examined by direct microscopy after Ziehl–Neelsen staining. After culture in BacT/ALERT 3D mycobacterial culture system, identification of the causative agents was performed using the GenoType MTCM system. Results: Culture positivity was higher in immunocompromised patients than in immunocompetent in both pulmonary and extrapulmonary samples. In smear-negative samples, culture positivity in immunocompromised patients (50%) was much higher than in immunocompetent patients (26.67%). Of 78 samples, four were NTMs (5.13%), including 1 M. avium , 1 M. abscessus, and 2 M. fortuitum , and 74 were Mycobacterium tuberculosis (MTBC). Two NTMs belonged to immunocompromised patients and two to immunocompetent individuals. The chance of Mycobacterial infection was higher in patients with a lower CD4 count. Both NTMs found in the immunocompromised group were from patients with a CD4 count<100. Conclusion: The present study showed that the MTBC is still the primary causative agent in pulmonary and extrapulmonary tuberculosis in this part of India.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89108604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant and Antibacterial Activity of Tanacetum spp. Essential Oil and Chemical Components Tanacetum spp.精油及其化学成分的抗氧化和抗菌活性
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.24
M. Alamholo
{"title":"Antioxidant and Antibacterial Activity of Tanacetum spp. Essential Oil and Chemical Components","authors":"M. Alamholo","doi":"10.52547/jommid.10.1.24","DOIUrl":"https://doi.org/10.52547/jommid.10.1.24","url":null,"abstract":"investigated the chemical composition and of on Methods : The aerial part of two plants were collected from Urmia Province, Iran. The essential oils were extracted using a Clevenger device. The antibacterial effect of essential oils was determined using the disc diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) by serial dilution method. free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) was examined. Chemical composition was measured using the Gas Chromatography-Mass Spectrometry (GCMS). Results: The major constituents in T. polycephalum and T. lingulatum essential oils were 1,8- cineole and camphor, respectively. The highest sensitivity (MIC of 0.312 μg mL - ( was observed with T. polycephalum against Bacillus subtilis. The lowest IC50","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90890683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates 溴化乙啶-琼脂侧轮法检测鲍曼不动杆菌临床分离株外排泵
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.36
A. Sepehr, Sepideh Fereshteh, N. Shahrokhi
{"title":"Detection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates","authors":"A. Sepehr, Sepideh Fereshteh, N. Shahrokhi","doi":"10.52547/jommid.10.1.36","DOIUrl":"https://doi.org/10.52547/jommid.10.1.36","url":null,"abstract":"adeC, were amplified by PCR. Finally, the presence of the AdeABC efflux pump was investigated using the agar ethidium-bromide cartwheel method (AEBCM). Results: According to the antibiogram test, all isolates were MDR. In the presence of efflux pump inhibitor, a reduced resistance for tetracycline was observed, but not for ciprofloxacin. The AdeABC efflux pump genes were detected in all isolates. An increase in the AdeABC pump activity in four isolates was confirmed using AEBCM. Conclusion: AEBCM, a fast and convenient tool for assessing the ethidium bromide secretion in various bacteria, provides a quick diagnosis and treatment of multidrug-resistant bacteria.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84434178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Streptococcus agalactiae-associated Urinary Tract Infections amongst Male Patients at a Tertiary Care Setting in Southwest India 无乳链球菌相关尿路感染的男性患者在三级护理设置在印度西南部
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.14
Asem Ali Ashraf, S. Govindan, Nagalakshmi Narasimhaswamy, Akshita Gupta
{"title":"Streptococcus agalactiae-associated Urinary Tract Infections amongst Male Patients at a Tertiary Care Setting in Southwest India","authors":"Asem Ali Ashraf, S. Govindan, Nagalakshmi Narasimhaswamy, Akshita Gupta","doi":"10.52547/jommid.10.1.14","DOIUrl":"https://doi.org/10.52547/jommid.10.1.14","url":null,"abstract":"females and 15 males (0.09%), had GBS bacteriuria. The patients were mainly in the 19-45 years old group (53%). The most typical comorbidity was type 2 diabetes mellitus (42%). The majority of the GBS isolates from male patients (>80%) were to benzylpenicillin, ceftriaxone, ampicillin, and cotrimoxazole. The isolates the least susceptibility to (26.1%)","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"181 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73532235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation, Molecular Identification, and Antibiotic Resistance Profile of Salmonella Typhimurium Isolated from Calves Fecal Samples of Dairy Farms in Hamedan 哈马丹奶牛场犊牛粪便中鼠伤寒沙门菌的分离、分子鉴定及耐药性分析
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.42
Maryam Najafi Asl, P. Mahmoodi, A. Bahari, A. Goudarztalejerdi
{"title":"Isolation, Molecular Identification, and Antibiotic Resistance Profile of Salmonella Typhimurium Isolated from Calves Fecal Samples of Dairy Farms in Hamedan","authors":"Maryam Najafi Asl, P. Mahmoodi, A. Bahari, A. Goudarztalejerdi","doi":"10.52547/jommid.10.1.42","DOIUrl":"https://doi.org/10.52547/jommid.10.1.42","url":null,"abstract":"Salmonellae are among the most common and the major causative agents of diarrhea in calves and humans. The present study was carried out to isolate and identify Salmonella in fecal samples of calves in industrial dairy farms of Hamedan and to determine antibiotic resistance profiles of the probable isolates. Methods: Salmonella were presumptively isolated based on the cultural characteristics and biochemical tests, and the identity of the isolates was further confirmed using genus- and serotype-specific PCR assays. Kirby-Bauer disk diffusion method was performed to determine antibiotic resistance profiles of the isolates. Results: Out of 120 stool samples collected from 8 industrial farms, 22 (18.33%) isolates possessing rfbJ , fliC and fljB genes were identified as Salmonella Typhimurium serotype. Antibiotic susceptibility test revealed all isolates (100%) were susceptible to gentamicin, amoxicillin-clavulanic acid, kanamycin, and ciprofloxacin and resistant against cotrimoxazole, cefazolin, and cefixime. Conclusion: To our knowledge, this study was the first report of Salmonella infection in Hamedan's dairy farms, indicating a relatively high prevalence rate of S . Typhimurium infection as the only detected serotype. Antibiotic resistance should also be considered a severe public health concern. Thus, effective hygiene measures should be adopted to prevent or reduce the infection, and monitoring antibiotic susceptibility is required to choose the drug of choice for treatment.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"163 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86677325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
No Detection of Crimean-Congo Hemorrhagic Fever Virus in Hard Ticks (Ixodidae) from a Highly Endemic Area in Southeast Iran 伊朗东南部高流行区硬蜱(硬蜱科)未检出克里米亚-刚果出血热病毒
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.30
Sahar Asadolahizoj, D. Saadati, M. Rasekh, F. Faghihi, M. Fazlalipour, Amir Sajad Jafari
{"title":"No Detection of Crimean-Congo Hemorrhagic Fever Virus in Hard Ticks (Ixodidae) from a Highly Endemic Area in Southeast Iran","authors":"Sahar Asadolahizoj, D. Saadati, M. Rasekh, F. Faghihi, M. Fazlalipour, Amir Sajad Jafari","doi":"10.52547/jommid.10.1.30","DOIUrl":"https://doi.org/10.52547/jommid.10.1.30","url":null,"abstract":"region of Sistan and Baluchestan Province, southeast of Iran. Methods: In this study, ticks were collected from 220 livestock, including 150 sheep, 50 goats, 20 cows in five counties of Sistan Province (Zabol, Zehak, Hirmand, Nimruz, and Hamun). The ticks were identified under a stereomicroscope according to valid morphological keys. A reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the CCHFV genome via amplifying the S segment. Results: Among 100 selected ticks, RT-PCR revealed no CCHFV infection. Conclusion: Although no ticks were positive for CCHFV, it should be recalled that Sistan and Baluchestan province is among the highly endemic CCHF foci. As a result, further investigation and larger sample sizes are required to confirm our outcome. According to the hypothesis that direct contact with viremic livestock is more significant than tick bites in the viral transmission, more serological and molecular screening should be performed on high-risk individuals, e.g., slaughterhouse staff, ranchers, farmers, and veterinarians in the Sistan region.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"163 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80308198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evolutionary Analysis of Mammalian ACE2 and the Key Residues Involved in Binding to the Spike Protein Revealed Potential SARS-CoV-2 Hosts 哺乳动物ACE2及其与刺突蛋白结合的关键残基的进化分析揭示了潜在的SARS-CoV-2宿主
Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-03-01 DOI: 10.52547/jommid.10.1.1
L. R. Lopes, Natália Carvalho de Lucca Pina, Antônio Carlos da Silva Júnior, Paulo Bandiera-Paiva
{"title":"Evolutionary Analysis of Mammalian ACE2 and the Key Residues Involved in Binding to the Spike Protein Revealed Potential SARS-CoV-2 Hosts","authors":"L. R. Lopes, Natália Carvalho de Lucca Pina, Antônio Carlos da Silva Júnior, Paulo Bandiera-Paiva","doi":"10.52547/jommid.10.1.1","DOIUrl":"https://doi.org/10.52547/jommid.10.1.1","url":null,"abstract":"Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV- 2) spilled over to humans via wild mammals, entering the host cell using angiotensin-converting enzyme 2 (ACE2) as receptor through Spike (S) protein binding. While SARS-CoV-2 became fully adapted to humans and globally spread, some mammal species were infected back. The present study evaluated the potential risk of mammals becoming hosts for SARS-CoV-2 through bioinformatics prediction based on ACE2 receptors.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89344847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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