Journal of Medical Microbiology and Infectious Diseases最新文献_第3页

Novel Mutations Associated with N-Gene Target Failure in SARS-COV-2 Genome in Iran, Case Series 与伊朗SARS-COV-2基因组n基因靶失败相关的新突变，病例系列

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-09-01 DOI: 10.52547/jommid.10.3.141

Ali Maleki, Zahra Fereydouni, Mahsa Tavakoli, Akram Ezani, Mirshamsedin Hosseini, Amir Hesam Nemati, Parastoo Yektay Sanat, T. Jalali, M. Pouriayevali, Mostafa Salehi-Vaziri

{"title":"Novel Mutations Associated with N-Gene Target Failure in SARS-COV-2 Genome in Iran, Case Series","authors":"Ali Maleki, Zahra Fereydouni, Mahsa Tavakoli, Akram Ezani, Mirshamsedin Hosseini, Amir Hesam Nemati, Parastoo Yektay Sanat, T. Jalali, M. Pouriayevali, Mostafa Salehi-Vaziri","doi":"10.52547/jommid.10.3.141","DOIUrl":"https://doi.org/10.52547/jommid.10.3.141","url":null,"abstract":"Precision tracking and monitoring viral genome mutations are critical during a viral pandemic such as COVID-19. As molecular assays for diagnosing numerous infectious agents are being developed, RT-PCR is still deployed as the gold standard for detecting SARS-CoV-2. Despite its proofreading capability, SARS-CoV-2, like other RNA viruses, adopts several changes in its genome. If these mutations, especially deletions, occur in the target areas of primers and probes, they will hinder molecular detection methods from identifying the given gene. The authors describe the cases in which, despite the lack of the N gene detection, the ORF1ab gene was discovered with a relatively low cycle of threshold (Ct). Following sequencing, changes were discovered in the annealing region of the forward and reverse primers and probes used in the SARS-CoV-2 detection kit. Among the most significant mutations is a large deletion of 15 nucleotides in the N gene, which has never been seen in prior variants. This highlights the importance of persistent monitoring of hypervariable regions in the SARS-CoV-2 genome through sequencing and updating the molecular detection kits during the COVID-19 pandemic.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73500052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 Variants Screening in Burkina Faso 布基纳法索SARS-CoV-2变体筛查

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-09-01 DOI: 10.52547/JoMMID.10.3.135

S. Soubeiga, Kinda Charlotte, A. Zouré, T. Compaoré, Sylvie Zida, Alidou Kagembega, T. Sagna, Charlemagne Dabiré, O. Ouédraogo, Nadiyah Lasisi, Alexia Kabore, Farida Milène Zida, Binta Fofana, Stéphane Somé, A. Nikiéma, D. Kambire, D. Zongo, I. Soulama, C. Sawadogo, Sandrine Gampini, H. Ouédraogo

引用次数: 1

How Will the COVID-19 Pandemic End? COVID-19大流行将如何结束?

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-09-01 DOI: 10.52547/jommid.10.3.146

E. Mostafavi

{"title":"How Will the COVID-19 Pandemic End?","authors":"E. Mostafavi","doi":"10.52547/jommid.10.3.146","DOIUrl":"https://doi.org/10.52547/jommid.10.3.146","url":null,"abstract":"More than two years after the COVID-19 pandemic outbreak, the status of the disease is still unclear to us. Up to August 1, 2022, the disease has killed about 6.4 million people worldwide, although the actual death toll is estimated to be three times more than the official death toll [1]. During this time, safe vaccines with high efficacy against the virus have been developed, methods of viral transmission and pathogenicity are better understood, and more effective treatments have been proposed that have helped better control the disease over time. A significant percentage of the world's population has acquired the infection at least once, more than 65% of the world's population has received at least one dose of a COVID-19 vaccine, and about 25% have received at least one booster dose of the COVID-19 vaccine. More than 12 billion doses have been administered globally, with 5.5 million added daily. However, the vaccine distribution is inequitable, and in low-income countries, only about 20% of people have received at least one dose of the vaccine [2]. Along with vaccines, antiviral drugs are another important achievement available. Many antiviral drugs, such as molnupiravir, fluvoxamine, and paxlovid have shown good therapeutic effects for COVID-19 patients [3].","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86399797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of Copper Nanoparticles Using Aqueous Thymus daenensis (Celak) Flora and Investigation of Its Antifungal Activity 利用胸腺菌群合成纳米铜及其抑菌活性研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-09-01 DOI: 10.52547/jommid.10.3.98

Katrin Ebrahimi, Sima Shir Ovand, A. Mohammedi, M. Nabi-Afjadi, Hamidreza Zalpoor, Farbod Bahreini

{"title":"Biosynthesis of Copper Nanoparticles Using Aqueous Thymus daenensis (Celak) Flora and Investigation of Its Antifungal Activity","authors":"Katrin Ebrahimi, Sima Shir Ovand, A. Mohammedi, M. Nabi-Afjadi, Hamidreza Zalpoor, Farbod Bahreini","doi":"10.52547/jommid.10.3.98","DOIUrl":"https://doi.org/10.52547/jommid.10.3.98","url":null,"abstract":", and energy-dispersive X-ray (EDX) were used to identify the synthesized nanoparticles. The antifungal activity of the synthesized copper nanoparticles was evaluated using the microdilution method. Results: After adding the extract to the copper sulfate solution, the solution color changed from light blue to yellowish-green. A maximum peak at the wavelength of 414 nm confirmed the copper nanoparticles formation. Scanning electron microscopy demonstrated the particle size ranging from 30 nm to 42 nm. The biosynthesized Cu-NPs had an inhibitory effect against Candida albicans , Fusarium solani , Aspergillus Niger , and Aspergillus flavus . Conclusion: Our findings demonstrated that T. daenensis aqueous extract acts as a reducer and stabilizer factor. We successfully synthesized Cu-NPs from copper sulfate using T. daenensis (Celak) flora aqueous extract according to the UV-Vis spectrum, FTIR, and SEM results. This research was the first report of Cu-NPs synthesized from an aqueous T. daenensis (Celak) flora extract. Our simple, quick, and inexpensive method for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87177048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ocular Manifestations in Children with COVID-19: Systematic Review and Meta-Analysis COVID-19患儿眼部表现:系统回顾和meta分析

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.58

N. Nasiri, H. Sharifi, T. Rahimi, Ghodsieh Sharif, A. Sharifi

引用次数: 0

Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People 龋齿及健康人链球菌和肠球菌的分布及耐药性研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.48

Shahad Fadhil Al-Taie, Muna T. Al- Musawi, Zaid S. Rasheed

{"title":"Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People","authors":"Shahad Fadhil Al-Taie, Muna T. Al- Musawi, Zaid S. Rasheed","doi":"10.52547/jommid.10.2.48","DOIUrl":"https://doi.org/10.52547/jommid.10.2.48","url":null,"abstract":"© The Author(s) Introduction: Antimicrobial resistance is one of the most significant challenges globally that threatens our ability to treat infectious diseases. This study investigated Streptococci and Enterococci in dental caries and healthy individuals and determined antibiotic resistance in the recovered bacteria. Methods: One hundred and twelve samples were collected, 56 from patients with dental caries and 56 samples from the teeth and saliva of healthy people. The samples were cultured on blood agar and purified on Mitis Salivarius agar. All isolates were identified by biochemical tests and Vitek 2 system and then examined for antibiotic susceptibility by disc diffusion method. Results: Streptococci and enterococci were the most isolated agents from dental caries and the teeth and saliva of healthy people. Streptococcus spp. comprised 48.61% of bacteria in dental caries and 28.40% in healthy individuals, while Enteroccus spp. was 22.22% in dental caries and 30.84% in healthy individuals. Streptococcus mutans and Streptococcus salivarius were more prevalent in dental caries and healthy individuals, respectively, while Enterococcus faecium was detected in both dental caries and healthy individuals. Also, there were significant differences between the number of streptococci isolated from healthy and caries people (P<0.05). In isolates recovered from healthy people, the streptococcal spp. exhibited high resistance to azithromycin (82.6%), cefixime and tetracycline (91.3%), and amoxicillin (60.8%). In contrast, enterococci were resistant to tetracycline (92%) and cefixime (76%). In dental caries isolates, the streptococcal and enterococcal spp. showed the highest resistance to amoxicillin (> 68%). Conclusions: The streptococcal and enterococcal spp. comprised the most isolated bacteria from healthy individuals and dental caries and exhibited multidrug resistance.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87982102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Insight into Nosocomial Infections: a Worldwide Crisis 对医院感染的新认识:一场全球性的危机

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.64

Elham Sheykhsaran, H. Ebrahimzadeh Leylabadlo, Farbod Alinezhad, H. Feizi, Hossein Bannazadeh Baghi

引用次数: 1

Congenital Cytomegalovirus in Algiers, Algeria, a Descriptive Case Series Study 阿尔及利亚阿尔及尔先天性巨细胞病毒:描述性病例系列研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.87

S. Aggoune

{"title":"Congenital Cytomegalovirus in Algiers, Algeria, a Descriptive Case Series Study","authors":"S. Aggoune","doi":"10.52547/jommid.10.2.87","DOIUrl":"https://doi.org/10.52547/jommid.10.2.87","url":null,"abstract":"© The Author(s) Cytomegalovirus (CMV) is an endemic ubiquitous herpes virus transmitted through saliva, urine, genital secretions, mononuclear blood cells, and transplanted tissue. It is a common infection with mild symptoms or asymptomatic in the immunocompetent individuals but can be severe in the immunocompromised individuals, e.g., HIV-infected individuals, transplanted and cancer patients, and fetuses. CMV is the leading cause of congenital viral infection and the leading non-hereditary cause of sensorineural hearing loss and mental retardation in early childhood. Here, we describe the clinical and laboratory monitoring of four congenital CMV (cCMV) cases referred to Pediatric Belfort Establishment in Algiers in 2019, 2020, and 2021. All the patients had developed signs and symptoms of postnatal CMV infection with intrauterine growth retardation. The clinical manifestations differed; some presented cytopenia with or without hepatosplenomegaly and others a clinical and biological cholestasis syndrome. All our patients had intrauterine growth retardation. A CMV PCR of a urine sample was positive. Treatment for six weeks based on ganciclovir, with a relay by valganciclovir.","PeriodicalId":34460,"journal":{"name":"Journal of Medical Microbiology and Infectious Diseases","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84673464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paraclinical Characteristics of Fatal and Recovered COVID-19 Cases: a Retrospective Study COVID-19死亡和康复病例的临床旁特征:回顾性研究

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.80

E. Goodarzi, M. Kakavand, K. Rashidi, S. Mamdohi, V. Momenabadi, Mojgan Navabi, Hamidreza Abolfathi, Z. Khazaei

引用次数: 0

Bacterial and Parasitic Intestinal Infections among Food Handlers in a Tertiary Care Hospital of Lucknow, Uttar Pradesh, India 印度北方邦勒克瑙一家三级医院食品处理人员肠道细菌和寄生虫感染

Journal of Medical Microbiology and Infectious Diseases Pub Date : 2022-06-01 DOI: 10.52547/jommid.10.2.54

Loveleena Agarwal, Amit Kumar Singh, A. Agarwal, Rajesh Kumar Yadav

引用次数: 0