Sports Medicine and Health Science最新文献

{"title":"Sulforaphane, Urolithin A, and ZLN005 induce time-dependent alterations in antioxidant capacity, mitophagy, and mitochondrial biogenesis in muscle cells","authors":"Neushaw Moradi,&nbsp;Sabrina Champsi,&nbsp;David A. Hood","doi":"10.1016/j.smhs.2024.03.011","DOIUrl":"10.1016/j.smhs.2024.03.011","url":null,"abstract":"<div><div>Efficient signal transduction that mediates mitochondrial turnover is a strong determinant of metabolic health in skeletal muscle. Of these pathways, our focus was aimed towards the enhancement of antioxidant capacity, mitophagy, and mitochondrial biogenesis. While physical activity is an excellent inducer of mitochondrial turnover, its ability to ubiquitously activate and enhance mitochondrial turnover prevents definitive differentiation of the contribution made by each pathway. Therefore, we employed three agents, Sulforaphane (SFN), Urolithin A (UroA), and ZLN005 (ZLN), which are activators of important biological markers involved in antioxidant signaling, mitophagy, and biogenesis, respectively. We investigated the time-dependent changes in proteins related to each mechanism in C2C12 myotubes. SFN treatment resulted in increased nuclear localization of the transcription factor Nuclear factor (erythroid-derived 2)-like 2 (Nrf-2) after 4 ​hour (h), with subsequent 2-fold increases in the antioxidant enzymes Nicotinamide Quinone Oxidoreductase 1 (NQO1) and Heme-Oxygenase-1 (HO-1) by 24 ​h and 48 ​h. Mitochondrial respiration and ATP production were significantly increased by both 24 h and 48 ​h. UroA showed a 2-fold increase in AMP-activated Protein Kinase (AMPK) after 4 ​h, which led to a modest 30% increase in whole cell mitophagy markers p62 and LC3, after 48 ​h. This was accompanied by a reduction in cellular Reactive Oxygen Species (ROS), detected with the CellROX Green reagent. Mitophagy flux measurements showed mitophagy activation as both LC3-II and p62 flux increased with UroA at 24-h and 48-h time points, respectively. Finally, AMPK activation was observed by 4 ​h, in addition to a 2-fold increase in Mitochondrial Transcription Factor A (TFAM) promoter activity by 24 ​h of ZLN treatment following transient transfection of a TFAM promoter-luciferase construct. Mitochondrial respiration and ATP production were enhanced by 24 ​h. Our results suggest that early time points of treatment increase upstream pathway activity, whereas later time points represent the increased phenotypic expression of related downstream markers. Our findings suggest that the spatiotemporal progression of these mechanisms following drug treatment is another important factor to consider when examining subcellular changes towards mitochondrial turnover in muscle.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 1","pages":"Pages 16-27"},"PeriodicalIF":2.3,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defecation after magnesium supplementation enhances cognitive performance in triathletes","authors":"Chen-Chan Wei ,&nbsp;M. Brennan Harris ,&nbsp;Mengxin Ye ,&nbsp;Andrew Nicholls ,&nbsp;Ahmad Alkhatib ,&nbsp;Luthfia Dewi ,&nbsp;Chih-Yang Huang ,&nbsp;Chia-Hua Kuo","doi":"10.1016/j.smhs.2024.04.001","DOIUrl":"10.1016/j.smhs.2024.04.001","url":null,"abstract":"<div><div>Constipation is correlated with diminished cognitive function, revealing a possible rectum-brain connection. In this counter-balanced crossover trial, 13 elite triathletes underwent a Stroop test to assess cognitive function and executive control. The Stroop test was conducted both with and without magnesium oxide intake, with a 1-week washout period between sessions. Oxygenation and blood distribution during the cognitive challenge were measured using Near-Infrared Spectroscopy (NIRS). Measurements were taken in both the prefrontal brain and the sub-navel region, where the highest glucose uptake was detected under the 18F-fluorodeoxyglucose Positron Emission Tomography (PET) scan. A significant reduction in completion time for the Stroop test was observed after defecation compared to the non-defecated condition (non-defecation: [27.1 ​± ​1.1] s; non-magnesium defecation: [24.4 ​± ​0.9] s; magnesium defecation: [23.4 ​± ​0.8] s, <em>p</em> ​&lt; ​0.05). Stroop test performance was improved in all (100%, 13/13) of the participants after magnesium-induced defecation and most (69%, 9/13) of the participants after non-magnesium-induced defecation. While no alterations in oxygenation and blood distribution were observed in the prefrontal brain during the Stroop test, decreased oxygenation levels were observed in the sub-navel region under both defecated conditions, without significant changes in blood distribution (<em>p</em> ​&lt; ​0.05). This data suggests an acute increase in oxygen consumption at this specific region. The result of this study suggests an unexplored causal link between the state of the rectum and cognitive performance. Magnesium supplementation to improved rectal emptying presents a novel application for optimizing cognitive function in athletes navigating intricate racing conditions.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 2","pages":"Pages 102-108"},"PeriodicalIF":2.3,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of incline walking on lower extremity and trunk mechanics in older adults","authors":"Seth Higgins ,&nbsp;D. Clark Dickin ,&nbsp;Dorice Hankemeier ,&nbsp;Meredith D. Wells ,&nbsp;He Wang","doi":"10.1016/j.smhs.2024.03.010","DOIUrl":"10.1016/j.smhs.2024.03.010","url":null,"abstract":"<div><div>Older adults are at an increased risk of developing knee osteoarthritis. High internal knee abduction moment during daily activities may elevate the risk of knee osteoarthritis. Incline walking exercise has been found to decrease knee abduction moment in healthy young adults. However, it is unknown if this occurs in healthy older adults. The purpose of this study was to quantify the internal knee abduction moment at different treadmill grades to determine if incline walking could reduce the knee abduction moment in healthy older adults. Twelve healthy older adult males walked on a treadmill at five incline grades (0%, 5%, 10%, 15%, and 20%) at 1.34 ​m⋅s^-1. The primary outcome variable was the internal knee abduction moment. A one-way repeated measures multivariate analysis of variance was performed to determine differences in the dependent variables among incline gradients. Peak knee abduction moment significantly decreased from level walking at all gradients in 10% increments (0%–10%, <em>p</em> ​&lt; ​0.001; 5%–15%, <em>p</em> ​&lt; ​0.002; and 10%–20%, <em>p</em> ​= ​0.04). A reduction in knee abduction moment during incline walking could result in decreased knee joint loading on the medial knee compartment. For older adults, who are looking to exercise to improve their health, incline walking may be beneficial to promote lower body strength and cardiovascular ability without inflicting further harm to the aging knee joints. However, because the frontal plane knee joint was of primary interest in this study, further research is needed to determine the effects of incline walking on other joints and in other planes of motion.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 1","pages":"Pages 56-60"},"PeriodicalIF":2.3,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular abnormalities of long-COVID syndrome: Pathogenic basis and potential strategy for treatment and rehabilitation","authors":"","doi":"10.1016/j.smhs.2024.03.009","DOIUrl":"10.1016/j.smhs.2024.03.009","url":null,"abstract":"<div><p>Cardiac injury and sustained cardiovascular abnormalities in long-COVID syndrome, i.e. post-acute sequelae of coronavirus disease 2019 (COVID-19) have emerged as a debilitating health burden that has posed challenges for management of pre-existing cardiovascular conditions and other associated chronic comorbidities in the most vulnerable group of patients recovered from acute COVID-19. A clear and evidence-based guideline for treating cardiac issues of long-COVID syndrome is still lacking. In this review, we have summarized the common cardiac symptoms reported in the months after acute COVID-19 illness and further evaluated the possible pathogenic factors underlying the pathophysiology process of long-COVID. The mechanistic understanding of how Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) damages the heart and vasculatures is critical in developing targeted therapy and preventive measures for limiting the viral attacks. Despite the currently available therapeutic interventions, a considerable portion of patients recovered from severe COVID-19 have reported a reduced functional reserve due to deconditioning. Therefore, a rigorous and comprehensive cardiac rehabilitation program with individualized exercise protocols would be instrumental for the patients with long-COVID to regain the physical fitness levels comparable to their pre-illness baseline.</p></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"6 3","pages":"Pages 221-231"},"PeriodicalIF":2.3,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666337624000374/pdfft?md5=3e88073339e8479f4a86716b61037f29&pid=1-s2.0-S2666337624000374-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140403436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of respiratory infections and SARS-CoV-2 is higher during contact phases in student rugby players – Lessons learnt from COVID-19 risk mitigation strategies–AWARE V","authors":"","doi":"10.1016/j.smhs.2024.03.005","DOIUrl":"10.1016/j.smhs.2024.03.005","url":null,"abstract":"<div><p>The incidence of acute respiratory infections (ARinf), including SARS-CoV-2, in unvaccinated student rugby players during phases from complete lockdown during the COVID-19 pandemic to returning to competition is unknown. The aim of the study was to determine the incidence of ARinf (including SARS-CoV-2) during non-contact and contact phases during the COVID-19 pandemic to evaluate risk mitigation strategies. In this retrospective cohort study, 319 top tier rugby players from 17 universities completed an online questionnaire. ARinf was reported during 4 phases over 14 months (April 2020–May 2021): phase 1 (individual training), phase 2 (non-contact team training), phase 3 (contact team training) and phase 4 (competition). Incidence (per 1 000 player days) and Incidence Ratio (IR) for ‘All ARinf’, and subgroups (SARS-CoV-2; ‘Other ARinf’) are reported. Selected factors associated with ARinf were also explored. The incidence of ‘All ARinf’ (0.31) was significantly higher for SARS-CoV-2 (0.23) vs. ‘Other ARinf’ (0.08) (<em>p</em> ​&lt; ​0.01). The incidence of ‘All ARinf’ (IR ​= ​3.6; <em>p</em> ​&lt; ​0.01) and SARS-CoV-2 (IR ​= ​4.2; <em>p</em> ​&lt; ​0.01) infection was significantly higher during contact (phases 3 ​+ ​4) compared with non-contact (phases 1 ​+ ​2). Demographics, level of sport, co-morbidities, allergies, influenza vaccination, injuries and lifestyle habits were not associated with ARinf incidence. In student rugby, contact phases are associated with a 3–4 times higher incidence of ARinf/SARS-CoV-2 compared to non-contact phases. Infection risk mitigation strategies in the contact sport setting are important. Data from this study serve as a platform to which future research on incidence of ARinf in athletes within contact team sports, can be compared.</p></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"6 3","pages":"Pages 252-259"},"PeriodicalIF":2.3,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666337624000337/pdfft?md5=774c9986f3bf749352a2d8ac689724dc&pid=1-s2.0-S2666337624000337-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The missing hydrogen ion, Part-3: Science and the human flaws that compromise it","authors":"Robert Robergs,&nbsp;Bridgette O'Malley,&nbsp;Sam Torrens","doi":"10.1016/j.smhs.2024.03.008","DOIUrl":"10.1016/j.smhs.2024.03.008","url":null,"abstract":"<div><p>The purpose of this research was to use a historical method and core principles from scientific philosophy to explain why mistakes were made in the development of the lactic acidosis construct. On a broader scope, this research explains what science is, why some scientists despite good intention, often get it wrong, and why it takes so long (decades) to correct these errors. Science is a human behaviour that consists of the identification of a problem based on the correct application of prior knowledge, the development of a method to best resolve or test the problem, completion of these methods to acquire results, and then a correct interpretation of the results. If these steps are done correctly there is an increased probability (no guarantee) that the outcome is likely to be correct. Thomas Kuhn proposed that you can understand what science is from how it has been performed, and from his essays he revealed a very dysfunctional form of science that he called ‘normal’ (due the preponderance of its presence) science. Conversely, Karl Popper was adamant that the practice of ‘normal’ science revealed numerous flaws that deviate from fundamental principles that makes science, science. Collectively, the evidence reveals that within the sports medicine and health sciences, as with all disciplines, errors in science are more frequent than you might expect. There is an urgent need to improve how we educate and train scientists to prevent the pursuit of ‘normal’ science and the harm it imparts on humanity.</p></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"6 2","pages":"Pages 193-199"},"PeriodicalIF":0.0,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666337624000362/pdfft?md5=1ef364e76d27434a680eba8f979ffd96&pid=1-s2.0-S2666337624000362-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140407848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of adipokines in exercise-induced inhibition of tumor growth","authors":"Yu Qian ,&nbsp;Zhenglong Bu ,&nbsp;Yang Qin ,&nbsp;Shiyuan Qian ,&nbsp;Lu Qin ,&nbsp;Siqi Zhou ,&nbsp;Qingda Wang ,&nbsp;Longjun Xian ,&nbsp;Lei Hu ,&nbsp;Yimei Xiong ,&nbsp;Yingying Zhang ,&nbsp;Chun Wang","doi":"10.1016/j.smhs.2024.03.006","DOIUrl":"10.1016/j.smhs.2024.03.006","url":null,"abstract":"<div><div>The integration of exercise prescriptions into cancer adjuvant therapy presents challenges stemming from the ambiguity surrounding the precise mechanism through which exercise intervention mitigates the risk of hepatocellular carcinoma (HCC) mortality and recurrence. Elucidation of this specific mechanism has substantial social and clinical implications. In this study, tumor-bearing mice engaged in voluntary wheel running exhibited a notable decrease in tumor growth, exceeding 30%. Microarray analysis revealed an upregulation of cytokine-related pathways as a potential explanation for this effect. The inclusion of granulocyte-macrophage colony-stimulating factor (GM-CSF) was found to enhance tumor cell proliferation, while the absence of GM-CSF resulted in a marked inhibition of tumor cell growth. The findings suggest that exercise-induced serum from mice can impede the proliferation of mouse tumor cells, with the adipokine chemerin inhibiting the growth factor GM-CSF. Additionally, exercise was found to stimulate chemerin secretion by brown adipose tissue. Chemerin suppression led to a reduction in the inhibition of tumor cell proliferation. The results of this study suggest that exercise may stimulate the release of adipokines from brown adipose tissue, transport them through the blood to the distant tumor microenvironment, and downregulate GM-CSF expression, alleviating tumor immunosuppression in the tumor microenvironment, thereby inhibiting at HCC progression. These findings provide a theoretical basis for incorporating exercise prescription into cancer treatment.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 2","pages":"Pages 143-156"},"PeriodicalIF":2.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11726049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myocarditis – A silent killer in athletes: Comparative analysis on the evidence before and after COVID-19 pandemic","authors":"","doi":"10.1016/j.smhs.2024.03.003","DOIUrl":"10.1016/j.smhs.2024.03.003","url":null,"abstract":"<div><p>Myocarditis is a rare cardiomyocyte inflammatory process, typically caused by viruses, with potentially devastating cardiac sequalae in both competitive athletes and in the general population. Investigation into myocarditis prevalence in the Coronavirus disease 2019 (COVID-19) era suggests that infection with Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is an independent risk factor for myocarditis, which is confirmed mainly through cardiovascular magnetic resonance imaging. Recent studies indicated that athletes have a decreased risk of myocarditis after recent COVID-19 infection compared to the general population. However, given the unique nature of competitive athletics with their frequent participation in high-intensity exercise, athletes possess distinct factors of susceptibility for the development of myocarditis and its subsequent severe cardiac complications (e.g., sudden cardiac death, fulminant heart failure, etc.). Under this context, this review focuses on comparing myocarditis in athletes versus non-athletes, owing special attention to the distinct clinical presentations and outcomes of myocarditis caused by different viral pathogens such as cytomegalovirus, Epstein-Barr virus, human herpesvirus-6, human immunodeficiency virus, and Parvovirus B19, both before and after the COVID-19 pandemic, as compared with SARS-CoV-2. By illustrating distinct clinical presentations and outcomes of myocarditis in athletes versus non-athletes, we also highlight the critical importance of early detection, vigilant monitoring, and effective management of viral and non-viral myocarditis in athletes and the necessity for further optimization of the return-to-play guidelines for athletes in the COVID-19 era, in order to minimize the risks for the rare but devastating cardiac fatality.</p></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"6 3","pages":"Pages 232-239"},"PeriodicalIF":2.3,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666337624000313/pdfft?md5=dc03c34d5d7a25284229faf0cceb3e71&pid=1-s2.0-S2666337624000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140273642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring urban-rural differences in 24-h movement behaviours among tunisian preschoolers: Insights from the SUNRISE study","authors":"Mohamed Amine Ltifi ,&nbsp;Olfa Turki ,&nbsp;Ghaith Ben-Bouzaiene ,&nbsp;Kar Hau Chong ,&nbsp;Anthony D. Okely ,&nbsp;Mohamed Souhaiel Chelly","doi":"10.1016/j.smhs.2024.03.004","DOIUrl":"10.1016/j.smhs.2024.03.004","url":null,"abstract":"<div><div>Little is known about differences in physical activity among children from urban and rural areas in low-to middle-income countries and some previous investigations revealed disparities in physical activity levels among children and adolescents residing in urban and rural environments. We aimed to: (i) assess the proportion of preschool-aged children (3.0–4.9 years) meeting the global movement guidelines, (ii) evaluate the feasibility of the methods for the SUNRISE study, (iii) examine gender- and urban-rural differences in health and behavior outcomes. Urban and rural location was based on national classifications. Physical activity (waist-worn ActiGraph); sleep duration, screen time and movement behaviors; Gross and fine motor skills (Lower body strength and mobility, Supine-Timed up and go [S-TUG], One-leg standing balance test, hand grip dynamometer, 9-hole peg-board test); and executive functions (visual-spatial working memory and inhibition) were assessed in 112 preschoolers (<em>n</em> ​= ​50 boys, 33 urban), (<em>n</em> ​= ​62 girls, 41 urban). The results showed that only 18% of children met all movement guidelines, with 53% and 41% meeting the recommendations for sedentary screen time and total physical activity, respectively. A large proportion of children (81%) met the recommended sleep duration of 10–13 ​hours (h) per day. There is a clear need to promote healthy movement behaviours among preschool-aged children through targeted interventions that address their unique challenges related to gender and urban/rural residence.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 1","pages":"Pages 48-55"},"PeriodicalIF":2.3,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140270721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise improves systemic metabolism in a monocrotaline model of pulmonary hypertension","authors":"Ganesha Poojary ,&nbsp;Sampara Vasishta ,&nbsp;R. Huban Thomas ,&nbsp;Kapaettu Satyamoorthy ,&nbsp;Ramachandran Padmakumar ,&nbsp;Manjunath B. Joshi ,&nbsp;Abraham Samuel Babu","doi":"10.1016/j.smhs.2024.03.001","DOIUrl":"10.1016/j.smhs.2024.03.001","url":null,"abstract":"<div><div>Exercise training in pulmonary arterial hypertension (PAH) has been gaining popularity with guidelines now recommending it as an important adjunct to medical therapy. Despite improvements in function and quality of life, an understanding of metabolic changes and their mechanisms remain unexplored. The objective of this study was therefore to understand the metabolic basis of exercise in a monocrotaline model of PAH.</div><div>24 male Wistar rats (age: 8–12 weeks and mean body weight: [262.16 ​± ​24.49] gms) were assigned to one of the four groups (i.e., Control, PAH, Exercise and PAH ​+ ​Exercise). The exercise groups participated in treadmill running at 13.3 ​m/min, five days a week for five weeks. Demographic and clinical characteristics were monitored regularly. Following the intervention, LC-MS based metabolomics were performed on blood samples from all groups at the end of five weeks. Metabolite profiling, peak identification, alignment and isotope annotation were also performed. Statistical inference was carried out using dimensionality reducing techniques and analysis of variance.</div><div>Partial-least-squares discrimination analysis and variable importance in the projection scores showed that the model was reliable, and not over lifting. The analysis demonstrated significant perturbations to lipid and amino acid metabolism, arginine and homocysteine pathways, sphingolipid (<em>p</em> ​&lt; ​0.05), glycerophospholipid (<em>p</em> ​&lt; ​0.05) and nucleotide metabolism in PAH. Exercise, however, was seen to restore arginine (<em>p</em> ​&lt; ​0.05) and homocysteine(<em>p</em> ​&lt; ​0.000 1) levels which were independent effects, irrespective of PAH.</div><div>Dysregulated arginine and homocysteine pathways are seen in PAH. Exercise restores these dysregulated pathways and could potentially impact severity and outcome in PAH.</div></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":"7 1","pages":"Pages 37-47"},"PeriodicalIF":2.3,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}