EAS Journal of Pharmacy and Pharmacology最新文献_第3页

In vitro Cytotoxic Activities of Catha edulis (Vahl) Forssk. Ex Endl. (Khat) Varieties from Kenya on Select Cancer Cell Lines Catha edulis (Vahl) Forssk的体外细胞毒活性Endl交货。(阿拉伯语)来自肯尼亚的肿瘤细胞系的选择

EAS Journal of Pharmacy and Pharmacology Pub Date : 2023-02-04 DOI: 10.36349/easjpp.2023.v05i01.002

L. Ngeny, L. Keter, B. Irungu, J. Kuria, N. Mwikwabe, J. Ondicho, R. Korir, P. Mwitari, F. Tolo, J. Orwa

{"title":"In vitro Cytotoxic Activities of Catha edulis (Vahl) Forssk. Ex Endl. (Khat) Varieties from Kenya on Select Cancer Cell Lines","authors":"L. Ngeny, L. Keter, B. Irungu, J. Kuria, N. Mwikwabe, J. Ondicho, R. Korir, P. Mwitari, F. Tolo, J. Orwa","doi":"10.36349/easjpp.2023.v05i01.002","DOIUrl":"https://doi.org/10.36349/easjpp.2023.v05i01.002","url":null,"abstract":"Khat (Catha edulis (Vahl) Forssk.) is a herb from the Celastraceae family (also known as qat, gaad, or miraa). The leaves and stems are used medicinally and for recreational purposes. The communities that grow khat have identified different varieties based on perceived appearance and quality. This study aimed to evaluate the cytotoxicity of khat varieties grown in the Meru and Embu Counties of Kenya. Field studies were undertaken in the markets and farms in Meru and Embu Counties of Kenya to document and purchase local khat varieties. Dried khat was extracted with a 1:1 v/v MeOH: CH2Cl2 solvent and water. Cytotoxicity of extracts was determined in vitro by MTT assay against four normal and cancer cells namely; HeLa ATCC® CCL-2™, HCC1395 ATCC® CRL-2324™, Hep2 ATCC® CCL-23™, and Vero E6 ATCC® CRL-1586™. The khat varieties identified were Muti Mutiiri, Mugwanthingi, Gicheru, Karimi ka Nthiya, Muguka, Black colombo asili, Black mbaine, and white. The aqueous extracts of black colombo asili and black mbaine displayed the highest cytotoxic activity against HeLa cell lines having IC50 37.15 ± 1.75 µg/ml and 38.31 ± 2.05 µg/ml, respectively. Muguka and Muti Mutiiri varieties were not cytotoxic to the Vero E6 cell line with CC50 > 100 µg/ml. 75% (12/16) of extracts were cytotoxic to the Vero E6 cell line with CC50 ˂ 100 µg/ml. This study demonstrated that there is variability in activities between the identified khat varieties. Toxicity was observed in vitro due to observed cytotoxicity to the Vero E6 cell line.","PeriodicalId":328128,"journal":{"name":"EAS Journal of Pharmacy and Pharmacology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116879306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toxicokinetics of Major Anthropogenic Indoor Air Pollutants and Their Effects on Human Health: A Review of Literature 室内主要人为污染物的毒性动力学及其对人体健康的影响:文献综述

EAS Journal of Pharmacy and Pharmacology Pub Date : 2023-01-28 DOI: 10.36349/easjpp.2023.v05i01.001

Ofor Casimir Chijioke, Ogiji Emeka Donald, Akuodor Godwin Chinedu, Shuneba Irene, Shu Elvis

引用次数: 0

Seasonal Impact on The Synthesis of Foliar Bioactive Components in Centella asiatica L. Urban 季节对积雪草叶面活性成分合成的影响

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-12-30 DOI: 10.36349/easjpp.2022.v04i06.002

M. S. Uddin, K. Alam

引用次数: 0

Preformulation Studies of Aceclofenac: Major Part of Formulation Strategy 乙酰氯芬酸的预处方研究:处方策略的主要部分

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-12-12 DOI: 10.36349/easjpp.2022.v04i06.001

Usha Arya, J. Malik

引用次数: 0

Phytochemical Screening and Antioxidant Property of the Methanol Extract of Spear Grass (Heteropogon contortus L.) 刺草甲醇提取物的植物化学筛选及抗氧化性能

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-09-30 DOI: 10.36349/easjpp.2022.v04i05.003

Ahomafor Joy E, Onyeloni Sunday, Ebene Akpevwe

{"title":"Phytochemical Screening and Antioxidant Property of the Methanol Extract of Spear Grass (Heteropogon contortus L.)","authors":"Ahomafor Joy E, Onyeloni Sunday, Ebene Akpevwe","doi":"10.36349/easjpp.2022.v04i05.003","DOIUrl":"https://doi.org/10.36349/easjpp.2022.v04i05.003","url":null,"abstract":"Plants are not only indispensable in health care but form the best hope of source for safe future medicines. In spite of the fact that now we have at our disposal a number of modern drugs, it is still genuinely urgent to discover and develop new therapeutic agents. The objective of the study is to determine the secondary metabolites and antioxidant activity of the methanol extract of Heteropogon contortus. The phytochemical analysis of the crude plant extract of Heteropogon contortus was performed using standard analytical method while the antioxidant activity of the extract was done using DPPH method of analysis. The study recorded yield of 9.22% for the methanol extract. Phytochemical analysis revealed the presence of seven secondary metabolites out of the nine metabolites screened for. The result of antioxidant activity of the crude extract showed good activity with an increase in the percentage inhibition on increase in concentration with the highest percentage inhibition of 57.16±53% at 500 mg/ml this was quite comparable to that of the standard (ascorbic acid) which had percentage inhibition at 500 mg/ml to be 67±32%. In conclusion the findings of the study showed that the methanol extract of H. contortus has an appreciable antioxidant activity which could be attributed to the secondary metabolites which it contains.","PeriodicalId":328128,"journal":{"name":"EAS Journal of Pharmacy and Pharmacology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130543274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hepatoprotective Effect of Leaves of Passiflora foetida Linn. (Passifloraceae) Against Paracetamol-Induced Liver Damage in Wistar Albino Rats 西番莲叶的保肝作用。对扑热息痛致Wistar白化大鼠肝损伤的研究

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-09-28 DOI: 10.36349/easjpp.2022.v04i05.002

B. Michel, O. C. Okou, T. Alassane, Kouadio Kouassi Patrice, N. S. Anthelme, T. Flavien

{"title":"Hepatoprotective Effect of Leaves of Passiflora foetida Linn. (Passifloraceae) Against Paracetamol-Induced Liver Damage in Wistar Albino Rats","authors":"B. Michel, O. C. Okou, T. Alassane, Kouadio Kouassi Patrice, N. S. Anthelme, T. Flavien","doi":"10.36349/easjpp.2022.v04i05.002","DOIUrl":"https://doi.org/10.36349/easjpp.2022.v04i05.002","url":null,"abstract":"Liver disease is one of the most common public health problems in the word. In this study, we investigate the possible hepatoprotective effect of the aqueous extract of Passiflora foetida (AEPF) using paracetamol-induced liver damage albino rats as animal model. The rats were divided in groups consisting of six animals each and treated for seven consecutive days as following: group I was the normal control and received distilled water, group II was the negative control treated with distilled water; group III served as the positive control and pretreated with 100 mg/kg of silymarin; groups IV, V and VI were pretreated with the AEPF doses of 400, 600 and 800 mg/kg respectively. At the seventh day, the rats from group II to VI were given a dose of 2 g/kg of paracetamol (PCM) and 24 hours after this treatment, blood samples and pieces of liver were collected for biochemical and histopathological analysis. Results showed that in group II, PCM increased significantly the activity of liver enzymes (ALT, AST, and PAL), the serum level of total and direct bilirubin and decreased significantly the serum level of total cholesterol, triglyceride and total protein in rats. Also, the liver tissue of the PCM-treated group exhibited severe acute centrilobular associated to periportal necrosis and haemorrhagic lesions in comparison to normal control rats. However, the biochemical and histopathological adverse effects of PCM were suppressed or reduced significantly by the pretreatment of rats with silymarin and AEPF dose dependently. These data indicated that Passiflora foetida possessed hepatoprotective effects similar to those of silymarin probably due to its saponins, phenols and flavonoids contents.","PeriodicalId":328128,"journal":{"name":"EAS Journal of Pharmacy and Pharmacology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127503083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Ethanol Extract of Centella asiatica on Acidity (pH) Levels of Rats Model Aerobic Vaginitis 积雪草乙醇提取物对大鼠有氧阴道炎模型酸性(pH)水平的影响

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-09-04 DOI: 10.36349/easjpp.2022.v04i05.001

O. Adar, F. Purwasari

{"title":"Effects of Ethanol Extract of Centella asiatica on Acidity (pH) Levels of Rats Model Aerobic Vaginitis","authors":"O. Adar, F. Purwasari","doi":"10.36349/easjpp.2022.v04i05.001","DOIUrl":"https://doi.org/10.36349/easjpp.2022.v04i05.001","url":null,"abstract":"Aerobic vaginitis (AV) is an infection in the vagina with a high inflammatory response, decreased Lactobacillus sp., increased pathogenic bacteria, increased parabasal cells, and increased acidity levels (pH). Increased pH levels in AV conditions are associated with reduced Lactobacillus in the vagina due to the dominance of aerobic or anaerobic bacteria that cause AV, including Streptococcus agalactiae, which can cause an increased risk of infection. Antibiotics are the mainstay of AV treatment but are resistant to several antibiotics, causing the need for other alternatives, one of which is the use of medicinal plants. The medicinal plant in this study is Centella asiatica plant which has been proven to have an anti-bacterial property. This study aims to compare pH levels before and after administration of ethanol extract of Centella asiatica on the acidity (pH) levels of Rats in AV model. This research is an experimental study use post-test control group only, consists of six groups Rattus norvegicus female. The first group was the negative control group (K-) rats. The second group was positive control rats (K+). The next are four groups of rats of AV models given various doses of ethanol extract of Centella asiatica, and each group consists of four rats, namely P1 (100 mg/kg). P2 group (200 mg/kg), P3 (400 mg/kg), and P4 (800 mg/kg). Paired T-Test showed results P-value is 0.000 (<0.005), stating that there was a significant difference between pH levels both before and after administration of ethanol extracts of Centella asiatica plant in rats model AV.","PeriodicalId":328128,"journal":{"name":"EAS Journal of Pharmacy and Pharmacology","volume":"341 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133119344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Characterization of Sustained Release Matrix Tablet of Pantoprazole 泮托拉唑缓释基质片的制备及表征

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-08-03 DOI: 10.36349/easjpp.2022.v04i04.001

Mukesh Kumar, Deeksha Singh, Vatan Chaudhary

引用次数: 0

An Approach to Fight Alzheimer's by Finding Its Causes and Risk Factors 通过寻找阿尔茨海默氏症的原因和危险因素来对抗它的方法

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-06-30 DOI: 10.36349/easjpp.2022.v04i03.003

H. Abushwereb, Mawada, S. Abukhrees

引用次数: 0

Phytochemical and Traditional Uses of Red Ginger: A Review (Zingiber officinale var. rubrum) 红姜的植物化学及传统用途综述

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-06-16 DOI: 10.36349/easjpp.2022.v04i03.002

R. Hendra, R. Rusdi, Ridho Asra, S. Misfadhila

引用次数: 1