Yemeni Journal for Medical Sciences最新文献

{"title":"Seroprevalence of Human Cytomegalovirus among Aborted Women in Aden, Yemen","authors":"Ali N.M. Gubran","doi":"10.20428/yjms.v17i1.2313","DOIUrl":"https://doi.org/10.20428/yjms.v17i1.2313","url":null,"abstract":"Human cytomegalovirus (HCMV) is one member of Herpesviridae. It can cause serious complications in pregnant women and their fetuses. So, this study aims to determine the prevalence of IgG and IgM against HCMV among aborted women and other related factors in Aden–Yemen. An analytical cross-sectional study was conducted on 88 aborted women. Blood samples were collected and serum HCMV IgG and IgM were analyzed using ELISA. The overall prevalence of IgG and IgM of HCMV infections were 77.6% and 83.3%, respectively. Neither HCMV IgG nor IgM seroprevalence had significantly influenced by age, number of abortions, education level, trimesters, and other factors. It can be concluded that the seroprevalence IgG antibodies of HCMV infections among aborted women were slightly lower than those reported globally whereas the seroprevalence IgM antibodies were higher than most of the previous studies that performed globally. Other causative factors may also contribute to abortion among aborted women. Further research is required to detect the HCMV and other causative agents of abortion in Aden and other southern governments of Yemen using adequate sample size and new advanced techniques.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":"137 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140251495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Antibiogram Patterns of Pseudomonas aeruginosa Isolated from Two Wastewater Treatment Plants in Aden Governorate-Yemen","authors":"Wadhah Mohamed Hadi Al-Qashbari, Mohamed Ali Ahmed Al-Baghdadi","doi":"10.20428/yjms.v17i1.2320","DOIUrl":"https://doi.org/10.20428/yjms.v17i1.2320","url":null,"abstract":"Introduction: Pseudomonas aeruginosa is a gram-negative rod-shaped obligatorily aerobic bacterium. This bacterium is most frequently associated with human infections and is regarded as an opportunistic pathogen, primarily causing nosocomial infections in immunocompromised patients. P. aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water as well as in other sources such as sewage treatment plants could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Therefore, the aim of this study was to determine the prevalence, antibiogram pattern and the multiple antibiotics resistant (MAR) of P. aeruginosa. Methods: The study was designed as a cross-sectional study, conducted between September 2022 and February 2023. A total of 193 wastewater samples, (97/193) & (96/193) were obtained from two region AL-Magari and Kaputa wastewater treatment plants (WWTPs), respectively. The wastewater samples were serially diluted and cultured on enrichment medium, then cultured on cetrimide agar as a selective medium for P. aeruginosa. The isolates were identified primarily based on cultural characteristics and then by biochemical tests. A panel of 32 antibiotics were used to determine the susceptibility patterns and the MAR. Results: The prevalence of P. aeruginosa was 33.7 % (65/193), while the statistical data revealed no a statistically significant difference between the prevalence of P. aeruginosa isolates from AL-Magari and Kaputa (P˃0.05). The study revealed the presence of antipseudomonal agents among the wastewater isolates of P. aeruginosa. Ciprofloxacin antibiotic has the highest activity against P. aeruginosa strains with the lowest resistant rate (7.7%), followed by Levofloxacin, Ofloxacin and Piperacillin/Tazobactam, (12.3%), (18.5%), and (20%), respectively. The MAR index ranged between 0.562 to 1. Conclusion: The study demonstrated that MAR P. aeruginosa were quite prevalent in the wastewater effluents of WWTPs in Aden governorate-Yemen with no statistically significant difference in resistant pattern between wastewater isolates from AL-Magari and Kaputa region to the antibiotics used in this study (P˃0.05); and this can lead to serious health risk for community, and natural vegetation where plants grow.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140250435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Phenotypic Methods in the Clinical Isolates for Biofilm Detection of Staphylococcus aureus and Escherichia coli in Mukalla city, Hadhramout, Yemen","authors":"B. Eidha, Amal Ali Bahakim, O. Riginal, Pen A Ccess","doi":"10.20428/yjms.v17i1.2123","DOIUrl":"https://doi.org/10.20428/yjms.v17i1.2123","url":null,"abstract":"Introduction: Biofilms represent a complex structure comprising prokaryotic cells, proteins, sugars, and DNA as majorconstituents. Bacteria can grow in biofilms and associated with human infections and considered to be highly resistant toantibiotics. There are various methods to detect biofilm production like tissue culture plate (TCP), tube method (TM), andCongo red agar method (CRA).Objectives: This study aimed to compare three methods for the detection of biofilm formation in the clinical isolatesStaphylococcus aureus and Escherichia coli.Methods: A total of 60 clinical isolates of S. aureus and E. coli were subjected to biofilm detection methods by TM, CRAand TCP.Results: Out of the total 60 clinical isolates of S. aureus and E. coli, TCP method detected 33 (55%) as strong, 15 (25%)as moderate and 12 (20%) as weak/non-biofilm producers. The sensitivity of TM and CRA was 56.3% and 60.4%, and thespecificity was 58.3% and 66.7% respectively. In our validation of the diagnostic biofilm production tests, the TCP methodwas superior to TM and CRA methods for biofilm detection.Conclusion: The study concluded that the TCP method is accurate and reliable method for the detection of biofilmformation in the clinical isolates S. aureus and E. coli compared to TM and CRA methods, and can be recommended as ageneral screening method for the detection of biofilm producing bacteria in clinical laboratories.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45072931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTI-SARS-COV-2 VACCINATION: ABOUT THE ALGERIAN EXPERIENCE","authors":"Imene Adouani, Zineb Benzaoui, N. Boulaacheb","doi":"10.20428/yjms.v17i1.2124","DOIUrl":"https://doi.org/10.20428/yjms.v17i1.2124","url":null,"abstract":"Introduction: The recent pandemic of the novel coronavirus-2 of severe acute respiratory syndrome (SARS-Cov-2 orCOVID-19), is one of the deadly diseases of the last twenty years.Objectives: Many efforts have been devoted to find solutions to fight COVID-19 pandemic as quickly and effectively aspossible. After the development of COVID-19 vaccines, several rumors about their effectiveness and safety spread all overthe world. The objective of the present study is to report data about the vaccination in Algeria and to document the variousside-effects notified by the Algerian population during the 24 hours following the administration of anti-COVID-19vaccines.Methods: An online observational retrospective survey targeting Algerian adults residing in the different Wilayas of thecountry has been conducted.Results: Nine hundred and ninety-four (994) Algerians participated in this survey. The age of the participants rangedbetween 18 and 78 years with an average of 34.75 ± 14.31 years. The sex ratio was 2.1 with female predominance. Theparticipants were from different Wilayas (provinces) especially: Bejaia (21.6%), Setif (17.8%), Algiers (8.5%), Batna(4.9%), etc. Almost half of the participants (46.9%) have a university degree. A total of 24% of participants werevaccinated between February 2021 and March 2022. Of these, 81.1% were fully vaccinated and 18.9% were partiallyvaccinated. Numerous vaccines were administered; Sinovac (50.4%), Janssen (16%), Sputnik (12.2%), AstraZeneca(10.1%), Sinopharm (5.9%), Pfizer (4.6%). More than half (62.3%) did not feel any signs after vaccination. Others (37.7%)claimed the appearance of certain effects including fever (21.6%), pain (16.5%), asthenia (8.1%), headache (7.2%) andothers (40.1%).Conclusion: According to the Algerian experience, in the majority of cases, anti-COVID-19 vaccines are devoid fromannoying effects. In some cases, it may be accompanied by some mild effects. However, vaccination is an important wayto prevent COVID-19 infection and the vaccine can be used safely.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43130895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Rapid Card Test And Enzyme Linked Immunosorbent Assay For The Detection Of Hepatitis B Surface Antigen In A Tertiary Care Hospital","authors":"Saheli Bhadra, S. Mohan, Tarana Sarwat, NG Gopeshwor, Sachin Sharma, D. Kakru","doi":"10.20428/yjms.v17i1.2122","DOIUrl":"https://doi.org/10.20428/yjms.v17i1.2122","url":null,"abstract":"Background: Hepatitis B virus (HBV) infection is one of the leading causes of death in developing countries. The most importantmarker for HBV diagnosis is detection of Hepatitis B surface antigen in blood.Objective: The aim of this study was to compare the analytical sensitivity of a rapid card test in comparison to Enzyme-LinkedImmunosorbent Assay (ELISA) which is considered a Gold Standard technique for the detection of HBsAg in blood samplesreceived for HBsAg test in serology section of Microbiology Department, Central Laboratory, Sharda Hospital.Methods: Cross-sectional observational study was conducted at the Department of Microbiology, School of Medical Sciences &Research for a period of 12 months from 1st November 2020 to 31st October 2021. The two different brands by which multipleparameters were analyzed were HEPACARD and Erba Lisa SEN HBsAg.Results: A total of 13939 blood samples were received for HBsAg diagnosis. For the study purpose, from the total of 13939samples, 386 were randomly selected to be included in the study with 193 positive samples and 193 negative samples. Thesensitivity and specificity of Rapid card were 95.83% and 100% respectively. The Positive predictive value and Negativepredictive value of Rapid card were 100% and 95.65% respectively for HBsAg detection.Conclusion: It can be concluded that, an ideal rapid test is a boon in time-saving situations. Since HBsAg screening by a rapidtest is easier, cost effective, time saving can be easily performed by any trained health care worker at any time of need. Hence,HBsAg screening can be preferable done by a Rapid card test followed by a supplemental ELISA and Polymerase chain reactionfor further confirmation. Good sensitivity and specificity of Immuno-chromatography test (ICT) for early detection of HBsAgwas observed.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43055245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nasal Carriage of Staphylococcus aureus among staff and Students of University of Sciences and Technology, Aden-Yemen","authors":"Mohammed A. A. Al-Baghdadi","doi":"10.20428/yjms.v16i1.2009","DOIUrl":"https://doi.org/10.20428/yjms.v16i1.2009","url":null,"abstract":"Background: Colonization of human nose by Staphylococcus aureus represent up to 30% of the human population. S. aureus must defeats the host’s defense mechanisms in order to colonize the nasal epithelial cells of human. Some factors such as bacterial interaction in human nose can prevent such colonization.Aim: The aim of current study was to determine the prevalence of nasal colonization by S. aureus among the staff and students of University of Science & Technology, Aden; as well as the associated risk factors for nasal carriage.Subjects and Methods: The type of study was an institution-based cross-sectional study which performed during the period from January to March 2020. The total samples were 1030 nasal swabs obtained from all the staff and students, the specimens were transported to the laboratory and the swabs were processed within 4 hrs. of collection.Results: The mean age of the study sample was 21.4 (±5.8 SD). The prevalence S. aureus isolated from nasal carriages was 31%. S. aureus nasal carriage show high frequency among age group 27-36 (32.4%) followed by those at age of 17-26 years (31%), however, there was no a statistically significant association in relation to the age groups (P=0.9). Regarding gender, it was considered a risk factor. S. aureus more common in male than female. The pattern of sleep, smoking, and frequent touching the nose were statistically associated with the S. aureus nasal colonization. Those who had nasal sensitivity was considered risk factor (RR=1.3) but with non-significant association with S. aureus nasal colonization (P=0.4).Conclusion: The current study revealed that community acquired was the potential colonizers of S. aureus. With the prevalence of 31% of the study sample, age, gender, and smoking, and other were potential risks. Regular screening of carriers is required for prevention of community acquired infections.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44336720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Nanoliposome of Labisia pumila Standardized Extract","authors":"Mohammed Ali Ahmed Saeed, Abdul Hakeem Memon, Armaghan Shafaei, Moh'd Shahrul Ridzuan Hamil, Amirin Sadikun, Zhari Ismail","doi":"10.20428/yjms.v16i1.2011","DOIUrl":"https://doi.org/10.20428/yjms.v16i1.2011","url":null,"abstract":"Background: Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila has been used traditionally for the treatment of several ailments such as gonorrhoea, dysmenorrhoeaand as tonics for females females.AimsAims: To investigate the standardization procedure of Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila extract (LPE) and evaluation of its nanoliposomes.Methods:Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila extract was standardized by high performance liquid chromatography (HPLC), and gallic acid, caffeicacid, rutin and 2,4 2,4-Di -tert tert-butylphenol were quantified in the extract. Then, the standardized extract was prepared as nanoliposomeusing soy bean phospholipid by the film method and after that was characterized by zetasizer, zeta potential, UV -Visspectrophotometer and FTIR techniques techniques.Results:For the standardization, the mean percentage recovery values of the concentration studied were 98.49 98.49±1.43, 97.01 97.01±2.04,97.7097.70±1.55 and 99.43 99.43±3.04 % for gallic acid, caffeic acid, rutin and 2,42,4-Di -tert tert-butylphenol, respectively. The accuracy values werebetween 95.06 and 104.86% for the marker compounds, while the corresponding precision values were betwee n 0.09 and 5.18% forwithinwithin-day and between between-day analysis, respectively. The average particle size for LLP was 174.20±4.58 nm with zeta potential ofparticles surface charge from −43.40 to − 44.40 mV. The polydispersity index was 0.19±0.02 and the morpholo gy and presence ofliposomes were further confirmed by transmission electron microscopy which revealed the presence of spherical liposomes of < 200nm.Conclusion:HPLC method for the simultaneous determination of selected marker compounds has been develop ed; the method wasreliable, repeatable and reproducible. The method was successfully applied in standardization of LPE. LPE was successfully pr eparedas nanoliposome using soybean phospholipid.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45134560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis B and C among Multi-Transfused Pediatric Thalassemic Patients in Sana'a City, Yemen: A Single-Center Retrospective Study","authors":"Ahmed S. Hudna, Adeeb A. Salah, Ahmed A. S. Almaqrami, Adam F. Senan, Farook A. Berman, Ibrahim M. Ali, Mohammed A. Al-Khalqi, Omar H. Al-Shoaibi, Waheeb M. Ali, Yahya A. Al-Arashani","doi":"10.20428/yjms.v16i1.2008","DOIUrl":"https://doi.org/10.20428/yjms.v16i1.2008","url":null,"abstract":"Objective: To determine the prevalence of hepatitis B and C infections and their association with repeated blood transfusions among thalassemic children attending the Yemen Society for Thalassemia and Genetic Blood Disorders (YSTGBD) in Sana’a city, Yemen.Methods: This retrospective cross-sectional study analyzed the medical records of 195 thalassemic children aged <18 years taken who were attending the YSTGBD from January 2008 to December 2018. Data about the gender and age of the child, time of thalas- semia diagnosis, frequency of monthly blood transfusions, hepatitis B virus (HBV) vaccination status, hepatitis B infection and hepatitis C virus (HCV) seropositivity were retrieved from the records into a data collection sheet. Data were then analyzed and summarized as frequencies and proportions. The association between the independent variables and infection with hepatitis B and/or hepatitis C was tested using the chi-square test.Results: The majority of thalassemic children attending the YSTGBD were males (58.5%), aged 8 years or older (81.0%), diag- nosed with thalassemia at 6 months of age or older (71.8%, were receiving ≤500 ml of transfused blood per month (88.7%) and had been vaccinated against HBV (72.8%). Regarding the serostatus for viral hepatitis, 15.4% (30/195) of children were seroposi- tive for the antibodies against HCV, 13.3% (26/195) were infected with hepatitis B as confirmed by HBsAg seropositivity, and 4.1% (8/195) were concomitantly seropositive for HBsAg and anti-HCV. Not getting vaccinated against HBV was significantly associated with infection with HBV (P <0.001), where 45.3% of unvaccinated children were seropositive compared to 1.4% of vaccinated children. In contrast, gender, age, age at thalassemia diagnosis and the quantity of monthly transfused blood were not significantly associated with infection with hepatitis B, anti-HCV seropositivity, or concomitant seropositivity for HBsAg and anti- HCV among thalassemic children.Conclusions: Viral hepatitis is a major problem for thalassemic children seeking healthcare in institutions of concern in Yemen, but it is rather difficult to assess the status of hepatitis C infection because of the lack of confirmatory tests. On the other hand, vaccination against HBV is significantly associated with a lower prevalence of hepatitis B among thalassemic children, highlighting the need for vaccinating all thalassemic children before enrollment in specialized centers of care. Thalassemic children should be screened for blood-borne viruses, including hepatitis viruses, before the establishment of regular blood transfusions.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45868239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graftless Maxillary Sinus Lifting with Simultaneous Dental Implant placement in Extraction Socket and Edentulous Ridge: A prospective Clinical Study","authors":"Mohammed Mansoor Albadani, Mohammed Ali Al-wesabi, Sadam Ahmed Elayah","doi":"10.20428/yjms.v16i1.2010","DOIUrl":"https://doi.org/10.20428/yjms.v16i1.2010","url":null,"abstract":"Aim: The present study aimed to evaluate the amount of bone formed after graftless maxillary sinus lifting procedure in edentulous ridges compared to newly extracted teeth sites when a simultaneous dental implant planned to be inserted.Subjects and methods: Twenty patients who needed dental implant in the posterior maxilla having a reduced vertical bone height were divided into two groups, fresh extraction socket group (10 cases) and patients with edentulous ridges at the site of indicated implant. All implants were from the same company with the same diameter and length. Dental Cone Beam Computed Tomography (CBCT) examination was performed to assess the bone gain height radiographically at 6 months after surgery.Results: The mean height of sinus elevation in extraction socket group was statistically significant higher than edentulous ridge group (4.02±0.84 and 2.63±1.56; respectively, P=0.023). Percentage change in the edentulous ridge group was (56.79±40.33), which is higher than that of extraction socket group (29.79±23.68) yet the difference was not significant (P=0.085).Conclusion: Dental implants at the site of pneumatized bone with simultaneous sinus lifting without bone graft showed a good and successful outcome in regard to bone gain and increased bone height with the fresh extraction socket cases.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44558384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP","authors":"Tarana Sarwat, S. Mohan, Satendra P. Singh, Dalip K. Kakru","doi":"10.20428/yjms.16.1.a1","DOIUrl":"https://doi.org/10.20428/yjms.16.1.a1","url":null,"abstract":"ABSTRACT \u0000Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance through multiple mechanisms. Infections due to extended spectrum β-lactamase (ESBL) producing isolates of P. aeruginosa continue to be a challenge for clinicians as these result in high mortality and morbidity due to antimicrobial resistance. The aim of this study was to determine the prevalence of Cefotaximase-Munich (CTX-M) producing strains of P. aeruginosa in Sharda hospital, Greater Noida. \u0000Methods: Strains of P. aeruginosa isolated from various clinical samples were subjected to phenotypic detection for ESBL production by disc combination method. Positive strains were then subjected to polymerase chain reaction (PCR) for detection of blaCTX-M gene. \u0000Results: Out of 166 isolates of P. aeruginosa, 54 (32.53%) were phenotypically confirmed to produce ESBL. Out of these 54 isolates, 39 (72.22%) were positive for blaCTX-M gene. Multidrug resistance was found in 70 (42.17%) isolates. Imipenem was the most effective drug with a sensitivity of 64.86% whereas aztreonam was found to be least effective with sensitivity of only 36.74%. \u0000Conclusion: Current study highlights the phenotypic and molecular characterization of CTX-M gene in P.aeruginosa in our hospital set-up. With judicious use of antimicrobials and strict infection control practices, it might be possible to limit the effect of these drug destroying enzymes. \u0000Keywords: CTX-M gene, Extended spectrum beta lactamases, Hospital acquired infections, multidrug resistance, \u0000Pseudomonas aeruginosa.","PeriodicalId":32479,"journal":{"name":"Yemeni Journal for Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45563070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}