Journal of Plant Molecular Breeding最新文献

{"title":"Comparative Transcriptome Analysis of GR2 and GL05136 in Mature-stage of Sugarcane","authors":"Luo Yefei, Ou Kewei, L. Qing, Yu Zhang, C. Qin, Zhu Pengjin, Zhou Quanguang, Pang Xinhua, P. Lv","doi":"10.5376/MPB.2021.12.0013","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0013","url":null,"abstract":"Sugarcane 'GR2' and 'GL05136' belong to late and early maturing high sugar varieties. Transcriptome sequencing was performed on the mixed samples of the leaves and stems of the two varieties to compare the differences in gene expression and to screen and clone genes that may be involved in sugar synthesis. Two comparison methods of interspecific at the same period and intraspecific at different periods were adopted, and four comparison groups were set up in the two varieties. KEGG and GO databases were used to analyze the overall transcriptome and the gene differential expression in the related fields of sugar synthesis. The results showed that there were 97 gene sequences of 5 enzymes related to sugar synthesis and several high-expression genes whose functions were unknown. Subsequently, the function of the high-expression genes could be verified by building a transgenic sugarcane validation system, improving the molecular mechanism theory of sugar metabolism, developing high-sugar molecular markers and even building high-expression transgenic varieties.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90969792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius","authors":"Yao Li, Yancen He, Yuhua Lin, Ting Wan, Meng-Qi Li, Zhiyong Chen","doi":"10.5376/MPB.2021.12.0001","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0001","url":null,"abstract":"Based on 3 generations transcriptome sequencing technology-PacBio SMRT single-molecule real-time sequencing method, supplemented with 2 generation sequencing method, a complete and accurate unigene library was constructed. After comparing with closely related species, the cellulose synthase gene ( CesA ) 4 , 7 , 9 gene sequences of Miscanthus lutarioriparius were spliced and named as MlCesA4 , MlCesA7 and MlCesA9 . Then, Bioinformatics analysis software was used to construct phylogenetic trees of proteins, to predict and analyze the phosphorylation sites of post-translational modifications of amino acids, and to predict and analyze conserved domains of proteins. The analysis results showed that the sequence lengths of MlCesA 4, 7, 9 genes were 2980 bp, 3310 bp, and 3208 bp, respectively, which were closely related to Sorghum bicolor and Zea mays ; cellulose synthase of Miscanthus lutarioriparius (MlCesA) MlCesA4 and MlCesA9 have 43 phosphorylation sites, MlCesA7 has 48 phosphorylation sites; MlCesA4 is an unstable protein with 6 transmembrane domains, 4 domains are outside the membrane and 3 are inside the membrane.; MlCesA7 and MlCesA9 are stable proteins, both of which have 8 transmembrane domains, 5 domains are outside the membrane and 4 are inside the membrane. The three are all hydrophilic proteins, and the secondary structure is dominated by α-helix and random coils.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81019827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapeseed Planting as Green Manure Improving Rice Growth and Production","authors":"Jifeng Zhu, Meiyan Jiang, L. Cao, Ming Zhang, Quanfeng Fei, Jinnan Jiao, Xirong Zhou, Weirong Wang","doi":"10.5376/mpb.2021.12.0037","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0037","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89279532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Analysis of PGM and SUS Gene Families in Leymus Chinensis Seed Transcriptome","authors":"Bo Wang, Ting Gong, Ran Li, Qi Wang, Jie Yang, Wei Liu, Zouzhuan Yang, Pan Zhang, Guofu Hu","doi":"10.5376/MPB.2021.12.0007","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0007","url":null,"abstract":"Leymus chinensis ( Leymus chinensis (Trin.) Tzvel.) is a good perennial forage with good palatability, high yield and high feeding value. In addition, because of its strong resistance, Leymus chinensis can improve the disadvantaged environment such as poor land, and it is an important species to protect grassland ecology in China. Therefore, in order to promote the germination of leymus chinensis seeds and explore the mechanism of Germination, this study used sterile water treatment of leymus chinensis seeds as control, exogenous GA3 as treatment conditions. Based on the analysis of pre-transcriptome data, it was determined that PGM and SUS genes in sucrose and starch metabolic pathways play a significant role in seed Germination. Members identification, conserved domains, and evolutionary trees were analyzed for both gene families using the bioinformatics method. The expression pattern of PGM and SUS genes were analyzed with FPKM value. The results showed that 7 members were selected from each of the the PGM and SUS families based on the data of Leymus Chinensis seeds transcriptome. The molecular weight of PGM genes ranged from 15 471.3 to 67 912.9 Da, and the isoelectric point value ranged from 4.45 to 6.31, showing weak acidity. PGM family in Leymus Chinensis seeds are hydrophilic stable proteins. The molecular weight of SUS genes ranged from 30 421.5 to 110 262.9 Da. The isoelectric point of SUS genes was neutral except LcSUS1,5 and 7 , while the other four genes were weak acidic. LcSUS4 , 6 were stable hydrophilic proteins, the rest were unstable hydrophilic proteins. The PGM and SUS gene families in Leymus chinensis seeds play an enzyme role in promoting sucrose and starch synthesis in sucrose and starch metabolism pathways. The expression pattern analysis of Leymus chinensis seeds showed that the genes of PGM and SUS gene families in GA3-treated Leymus chinensis seeds promoted the germination of Leymus chinensis seeds by regulating the sucrose and starch pathways with different expression levels. genes is found to be between 4.45~6.31 through the analysis of the isoelectric point, and the average value is about 5.67, indicating that it mainly plays a role in the environment of weak acid cells. The prediction of the instability coefficient showed that the values were all less than 40, so it could be presumed to be a stable protein. The average hydrophilic coefficients were all less than 0, indicating that the proteins of these seven genes were hydrophilic proteins. The results showed that the seven LcPGM proteins were different in predicting the length of amino acid sequence and the physicochemical properties of the proteins, suggesting that LcPGM proteins had different biological properties.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80547259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Analysis of Resistance to Soybean Mosaic Virus and Variation in Resistance Allelic Excavation","authors":"Wang Weiwei, Lai Wei, Yuan-Zhou Zhiyuan, Guixing Zhao, Weiwei Bi, L. Lijun","doi":"10.5376/MPB.2021.12.0012","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0012","url":null,"abstract":"Soybean Mosaic Virus (SMV) is one of the most important soybean diseases in the world, and it seriously affects soybean yield and quality. In this paper, 405 SSR molecular markers were used in genome-wide scan to analysis 327 soybean varieties in the North of China. The purpose was to search the genetic loci and excellent resistance genes related to SMV1 and SMV3, and to reveal the differences between the genetic models of varieties. The results confirmed that the genetic differences among the tested varieties were large. Through the association analysis of the population genome-wide association (GWA) and linkage group association (LGA), 14 highly significant sites related to SN1 and SN3 were found. There are three sites related to SN1 (Sat-297, sat-296, sat-154) which are coincident with or adjacent to the resistance sites found by predecessors; while there are two at SN3 (Sat-154 and sat-726) which are coincident with previous studies, and sat-154 is a resistance site shared by SN1 and SN3. 7 genes related to resistance were obtained, of which Glyma13g25420 and Glycma13g25440, Glyma14g08700 and Glyma14g08710 has resistance genes on the NBS-LRR domain; Glyma14g08900, Glyma14g08910, and Glyma14g08920 have PLP2 (Patation-like Protein) resistance genes. This study provides an important reference for SMV resistance research and technical support for soybean resistance breeding.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86179097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of SRAP and CDDP Markers in Genetic Diversity Analysis of Morinda officinalis How","authors":"M. Luo, F. Liu, Weili Zhang, Cui Juntao, Donghui Li, Jiyan Chen, Zhangyong Dong","doi":"10.5376/mpb.2021.12.0020","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0020","url":null,"abstract":"The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing. The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software, cluster analysis was carried out according to UPGMAmethod, and the results of two kinds of molecular markers were compared and analyzed. 28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively, of which the number of polymorphic bands was 68 and 63 respectively, the polymorphism ratio (PPB) was 48.6% and 75.9% respectively, the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95, and the average genetic similarity coefficient was 0.83 and 0.69 respectively. When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71, the four varieties of Guangning special type, Guangning special grafting type, local large leaf type and local lobular type were grouped into one group. SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How.. There are some differences between SRAP and CDDP, so the combined analysis of the two kinds of markers can more accurately reveal the genetic relationship between Morinda officinalis How. germplasm. The results of CDDP clustering were more consistent with those of combined analysis, indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How. than SRAP.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85621928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of Pi-kh Co-dominant Marker for Rice Blast Resistance Gene","authors":"Shifei Sang, Junyi Wang, Jing-Jing Zhou, Mengyu Cao, Yanan Wang, Jianqiang Zhang, Shengdong Ji, Wenling Zhang","doi":"10.5376/MPB.2021.12.0004","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0004","url":null,"abstract":"Rice, as one of the staple food crops in the world, is reduced by 10~30% every year due to the damage of rice blast. However, the application of marker assistant selection (MAS) with rice disease resistance genes can be effectively, accurately and rapidly used to breed rice blast resistant varieties. The rice blast resistance gene Pi-kh has been proved to have broad-spectrum resistance to the rice blast. Therefore, the selection and utilization of Pi-kh gene by MAS in rice disease resistance breeding possess high application value for improving rice blast resistance. In this study, based on two InDel differences in Coding sequence (CDS) region of Pi-kh gene, a co-dominant functional molecular marker was developed at the conservative position of the sequence, which was named ZJPikh. The marker was used in the disease resistance breeding with rice blast susceptible variety “Yudao 518” as female parent and rice blast resistant variety “Shida 68” as male parent. The genotype of gene Pi-kh related with rice blast resistance in F2 isolated population and high generation plants were verified by pedigree method and MAS. A new rice blast resistant third-grade variety “Yudao 58” with Pi-kh was successfully bred. At the same time, the Pi-kh genotypes of 21 main rice varieties were identified, which provided technical support for the targeted and efficient breeding of blast resistant varieties carrying Pi-kh gene through MAS technology. Our research provides convenience for identifying rice blast resistance germplasm resources and molecular marker-assisted disease resistance breeding.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78145379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomerase Activity of Apple Plantlets in Vitro Kept Relative Stable after Conserved for Years","authors":"Chenchen Liang, Yajie Liu, Li Wang, G. Du, Xiaoxin Shi","doi":"10.5376/mpb.2021.12.0035","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0035","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75571627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and Tissue Expression Analysis of Calcium-dependent Protein Kinase Gene BsCDPK1 in Bletilla striata","authors":"Shuangshuang Wang, G. Zeng, Quanli Dou, Bo-Lin Zhang, Qinghua Tian","doi":"10.5376/mpb.2021.12.0038","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0038","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83677203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic Analysis of Catalase Gene Family of Arabidopsis and Maize","authors":"Shengnan Ge, Yulei Wei, Jinjie Zhang, Wenjing Shao, Jiaxin Li, D. Ding, Meng Liu, Jingyu Xu","doi":"10.5376/mpb.2021.12.0034","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0034","url":null,"abstract":"Catalase (CAT) is an antioxidant enzyme, which plays a key role in plant development and abiotic stress response. In this study, three maize ZmCATs and three Arabidopsis AtCATs genes were identified by screening NCBI and phytozome databases. The protein characteristics, evolutionary relationship, gene structure, protein secondary and tertiary structure, gene expression at different developmental stages or under abiotic stress treatment were analyzed. The results showed that there were some similarities between CAT families from the two species. The proteins encoded by ZmCATs and AtCATs genes were hydrophobic. According to phylogenetic tree analysis, CATs gene family was divided into two subfamilies. ZmCATs and AtCATs both contain two conserved domains, and the secondary structures of ZmCATs and AtCATs protein are mainly α-helix and random coils. The cluster analysis of ZmCATs expression in different growth and development stages showed that ZmCAT1 , ZmCAT2 and ZmCAT3 were highly expressed in maize growth and development stage. Under abiotic stress, the expression of ZmCATs gene was significantly affected by temperature stress. The results of this study provide a theoretical basis for exploring the function of CAT in maize and other crops.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82036587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}