Application of SRAP and CDDP Markers in Genetic Diversity Analysis of Morinda officinalis How

Abstract

The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing. The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software, cluster analysis was carried out according to UPGMAmethod, and the results of two kinds of molecular markers were compared and analyzed. 28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively, of which the number of polymorphic bands was 68 and 63 respectively, the polymorphism ratio (PPB) was 48.6% and 75.9% respectively, the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95, and the average genetic similarity coefficient was 0.83 and 0.69 respectively. When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71, the four varieties of Guangning special type, Guangning special grafting type, local large leaf type and local lobular type were grouped into one group. SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How.. There are some differences between SRAP and CDDP, so the combined analysis of the two kinds of markers can more accurately reveal the genetic relationship between Morinda officinalis How. germplasm. The results of CDDP clustering were more consistent with those of combined analysis, indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How. than SRAP.