Journal of Plant Molecular Breeding最新文献_第7页

Identification and Bioinformatics Analysis of KNOX Gene Family in Wheat (Triticum aestivum L.) 小麦KNOX基因家族的鉴定及生物信息学分析

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/MPB.2021.12.0019

Ningning Song, Huihui Liang, Yi An, Shenglong Bai, Feifei Ma, Zhen Zhang, Hao Li, Yun Zhou, Guang-yan Guo, Chunpeng Song

{"title":"Identification and Bioinformatics Analysis of KNOX Gene Family in Wheat (Triticum aestivum L.)","authors":"Ningning Song, Huihui Liang, Yi An, Shenglong Bai, Feifei Ma, Zhen Zhang, Hao Li, Yun Zhou, Guang-yan Guo, Chunpeng Song","doi":"10.5376/MPB.2021.12.0019","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0019","url":null,"abstract":"KNOTTED-like homeodomain ( KNOX ) gene family is a transcription factor encoding homeobox protein, which plays an important role in plant growth and morphogenesis. However, little information is available on the KNOX gene family in wheat ( Triticum aestivum L.). In this study, 36 KNOX genes with KNOX1 or KNOX2 domain distributed on 18 chromosomes were identified from wheat genome using bioinformatics methods. The phylogenetic evolution, gene structure, protein domains, cis-acting elements and gene expression patterns were analyzed in the present study. Based on the phylogenetic tree, the 36 TaKNOX genes were divided into two major subclasses, Class I and Class II, and the two subclasses were further divided into five evolutionary branches. Most TaKNOX genes contain four typical conserved protein domains: KNOX1, KNOX2, ELK and HOX. Some cis-acting elements are associated with hormonal, plant development and stress in TaKNOX promoters. The analysis result of transcriptome data from wheat different tissue showed that Class I KNOX genes had obvious tissue specificity, while Class II KNOX genes expressed widely in different wheat tissues. The study results provide important information for future analysis of the regulation and functions of the TaKNOX gene family.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87201146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tibet The Seed Germination Characteristics Under Low Temperature and Spring Sowing Study of 8 Rapeseed Varieties 西藏8个油菜籽品种低温和春播条件下种子萌发特性研究

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/MPB.2021.12.0016

Jifeng Zhu, Xirong Zhou, Jianxia Jiang, Junying Zhang, Li Yanli, Liyong Yang, Weirong Wang, Meiyan Jiang

{"title":"Tibet The Seed Germination Characteristics Under Low Temperature and Spring Sowing Study of 8 Rapeseed Varieties","authors":"Jifeng Zhu, Xirong Zhou, Jianxia Jiang, Junying Zhang, Li Yanli, Liyong Yang, Weirong Wang, Meiyan Jiang","doi":"10.5376/MPB.2021.12.0016","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0016","url":null,"abstract":"To explore the low temperature germination characteristics of rapeseed, 8 main cultivars were selected for germination experiments in incubators at 20°C, 16°C, 12°C and 8°C, respectively, to measure and analyze the germination potential, germination rate, root length, bud length, etc. The results showed that there was significant difference in the germination potential of tested rapeseeds at temperatures. And the low temperature of 8°C was significantly reduced the germination potential. With the decrease of germination temperature, the growth of roots and buds were inhibited significantly. There were significant differences both in temperatures and varieties. The germination potential, root length and bud length could be used as the main index to evaluate seed germination of rapeseed under low temperature. In addition, to screen varieties suitable for spring sowing as green manure, the spring sowing test was carried out. The study found that the fresh grass yield of “Huyouzao No.1” was 1712.53 kg/m2, which was much higher than other 7 varieties. Its relative root length was second only to “Huyou17” and its relative bud length next to “Huyoufei No.1” at 8°C, respectively. The results can provide a reference for early spring sowing of rapeseed as green manure.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81990067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity and Relationship Analysis of 21 Walnut Varieties Based on SSR Markers 基于SSR标记的21个核桃品种遗传多样性及亲缘关系分析

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/MPB.2021.12.0009

Ling-ling Xu, Yi Wang, Xiaolong Yuan, B. Lu, Jia-bo Hao, Yu Zhang, Jun-Hui Ma, Fei Wang

引用次数: 0

The Regulation of Brassinolide Biosynthesis and Physiological Effects in Grape Berries 油菜素内酯在葡萄果实中的生物合成调控及生理效应

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2021.12.0033

Jiajia Li, Xiangyi Li, Lei Wang, Shiping Wang

引用次数: 2

Genotyping of 10 Grain Shape Genes in High Quality Xianindica Varieties by Molecular Marker 优质籼型品种10个粒形基因的分子标记分型

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2021.12.0029

Lei Zhou, Erjing Li, Huashan Xu, Zhijue Chen, Guocai Yang, Kai Liu, A. You

引用次数: 0

Screening and Bioinformatics Analysis of Proteins Interacting with StMAPKK1 in Potato by Yeast Two-Hybrid System 马铃薯StMAPKK1互作蛋白的酵母双杂交筛选及生物信息学分析

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2020.11.0003

Yu-Huang Liao, Fangfang Wang, Xi Zhu, Ning Zhang, H. Si

{"title":"Screening and Bioinformatics Analysis of Proteins Interacting with StMAPKK1 in Potato by Yeast Two-Hybrid System","authors":"Yu-Huang Liao, Fangfang Wang, Xi Zhu, Ning Zhang, H. Si","doi":"10.5376/mpb.2020.11.0003","DOIUrl":"https://doi.org/10.5376/mpb.2020.11.0003","url":null,"abstract":"Mitogen-activated protein kinase (MAPK) cascade reaction is one of important and complex signal networks involved in plant growth and development, hormone and stress response. As one of its main members, mitogen-activated protein kinase kinase (MAPKK) is located in the middle of the cascade reaction and plays a key role in signal collection and divergence. There were shown that potato ( Solanum tuberosum L.) StMAPKK1 (PGSC0003DMT400000744) gene responded to drought stress. Therefore, StMAPKK1 gene was firstly selected to screen its interacting protein. In this study, the bait vector pGBKT7-StMAPKK1 was constructed by homologous recombination and used to screen potato cDNA library by yeast ( Saccharomyces cerevisiae ) two-hybrid system. Five StMAPKK1-interacting proteins, hydrolase (hydrolyzing O-glycosyl compound), RING-H2 subgroup RHE protein, cyanate hydratase, ARF GTPase activator, and a C2 domain-containing protein, were obtained through this screening and were identificated by bioinformatics analysis, and the interaction was verified by small-scale hybridization verification. The results provided theoretical basis for further study on the signal pathway and biological function of potato StMAPKK1.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"2000 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88316396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of SSR Markers Associated with Polima CMS Fertility Restorer in Brassica napus L. and Its Application 甘蓝型油菜Polima CMS育性恢复相关SSR标记的开发及其应用

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/MPB.2021.12.0017

Tonghua Wang, Bao Li, Yiming Guo, Yaoting Zhang, Xinhong Liu, L. Fan, Lianghuan Qu, Li-Ming Deng, Mei Li

{"title":"Development of SSR Markers Associated with Polima CMS Fertility Restorer in Brassica napus L. and Its Application","authors":"Tonghua Wang, Bao Li, Yiming Guo, Yaoting Zhang, Xinhong Liu, L. Fan, Lianghuan Qu, Li-Ming Deng, Mei Li","doi":"10.5376/MPB.2021.12.0017","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0017","url":null,"abstract":"The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability of occurrence of maternal false hybrid due to the fertility of Polima CMS (Pol CMS) line greatly affected by the ambient temperature, therefore, the detection of maternal false hybrids is the key to purity identification. In present study, the SSR markers were designed based on the flanking sequences of Pol CMS restorer gene Rfp , and application of SSR markers in hybrid seed purity test of rapeseed. The main results were as follows: the sequences of Rfp were subjected to basic local alignment search tool queries against the Brassica rapa genome to determine chromosome positions, and 32 SSR markers were developed based on the upstream and downstream sequences of Rfp . Among the 32 SSR markers, one codominant marker was polymorphic between Pol CMS sterile and restorer line, the PCR product length was 162 bp in Pol CMS sterile lines, and 184 bp in restorer lines. Based on the PCR sequence, two insert fragments (15 bp and 7 bp) were identified in Pol CMS restorer lines. The SSR marker co-segregated with plant fertility of individuals in the “fengyou737” F 2 population. The purity of “fengyou737”, “fengyou320” and “fengyou958” were identified by means of PCR-SSR markers and field-planting, and the results of two ways were highly consistent, indicated that the codominant SSR marker tightly linked to Rfp , and the marker can detect paternal and maternal false hybrids. The present findings will provide technical assistance for hybrid seed purity test and genetic diversity analysis in rapeseed.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77236986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome wide Association Analysis 全基因组关联分析

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2021.12.0027

Xiaocong Cao, Chao-jun Zhang, Haoqi Gou, Xiaoyan Wang, Kaikai Qiao, Q. Ma, Guiyin Zhang, S. Fan

引用次数: 0

Breeding of Drought resistance and Salt tolerance, Suitable for Machine Picking New Cotton Variety Hengmian HD008 适合机器采摘的棉花新品种恒棉HD008的选育

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2021.12.0036

Maohua Dai, Liying Liu, Y. Li, Chao Liu, Zhenliang Wu

引用次数: 0

Cloning and Expression Analysis of EdAGPL1 in Eleocharis dulcis 桃蒜EdAGPL1基因的克隆与表达分析

Journal of Plant Molecular Breeding Pub Date : 2021-01-01 DOI: 10.5376/mpb.2021.12.0031

Fanglian He, Zuyang Qiu, Weiqing Dong, Lili Liu

引用次数: 0