Environmental Research最新文献

{"title":"Associations between dietary patterns and environmental contaminants in children from the Norwegian Environmental Biobank: A cross-sectional analysis","authors":"Mari Mohn Paulsen ,&nbsp;Line Småstuen Haug ,&nbsp;Amrit Kaur Sakhi ,&nbsp;Cathrine Thomsen ,&nbsp;Anne Lise Brantsæter","doi":"10.1016/j.envres.2025.121871","DOIUrl":"10.1016/j.envres.2025.121871","url":null,"abstract":"<div><div>Diet is a well-known source of environmental contaminants. This cross-sectional study explored children's dietary patterns and their association with contaminant exposure in the Norwegian Environmental Biobank. Parent-completed questionnaires provided data on children's frequency of intake of 36 foods and beverages, and socio-demographics. Several environmental contaminants were measured in urine, plasma, and whole blood from the children. Data were available for 658 children aged 7–14 years. Two dietary patterns, denoted \"healthy\" and \"unhealthy,\" were identified using data driven factor analysis. We examined associations between child dietary patterns and contaminant concentrations using linear regression, adjusting for participant characteristics. The healthy pattern was positively associated with plasma perfluorononanoate (PFNA) concentration, whereas the unhealthy pattern was positively associated with urinary bisphenol A (BPA) concentration. Likelihood of exceeding health-based guidance value (HBM-GV) was examined using generalized linear model Poisson regression. The sum of the four most prevalent per- and polyfluoroalkyl substances (Ʃ4PFAS) increased with higher healthy pattern scores, with a relative risk (RR) of 1.18 (95 % CI: 1.06, 1.31) per standard deviation. Multiple regression analyses showed that egg and fish/shellfish consumption were associated with higher Ʃ4PFAS, with 4 % and 5 % higher concentrations per additional weekly serving, respectively (<em>P</em> &lt; 0.001). For the sum of urinary di(isononyl)cyclohexane-1,2-dicarboxylate (ƩDINCH), 10 % higher concentration was observed per weekly serving of sweets/desserts. The urinary BPA concentration was 3 % higher per extra weekly serving of fruit juice (<em>P</em> = 0.001). These results highlight the need for societal measures to reduce contaminant exposure through food.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"280 ","pages":"Article 121871"},"PeriodicalIF":7.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diatom-dinoflagellate dynamics in marine ranching ecosystems under seasonal shifts and interannual climatic conditions","authors":"Shike Gao ,&nbsp;He Zhang ,&nbsp;Jikun Lu ,&nbsp;Guanghui Fu ,&nbsp;Lu Lu ,&nbsp;Shuo Zhang ,&nbsp;Chunmei Gao","doi":"10.1016/j.envres.2025.121869","DOIUrl":"10.1016/j.envres.2025.121869","url":null,"abstract":"<div><div>Global climate events like the El Niño-Southern Oscillation (ENSO) significantly influence ecological dynamics in coastal ecosystems, particularly engineered ones. The health of primary producers such as diatoms and dinoflagellates are vital for engineered ecosystem balance, and understanding their dynamics is essential for effective conservation strategies. This study utilizes long-term monitoring data from 2003 to 2022, employing generalized additive models (GAM) to analyze the spatio-temporal variations of diatom and dinoflagellate abundance in Haizhou Bay, with a focus on the impacts of interannual climate events. The results showed that diatom abundance peaked in October, with significantly lower values in May, indicating strong seasonal variation (<em>P</em> &lt; 0.01). In contrast, dinoflagellate abundance was highest in October and showed less pronounced seasonal differences. Interannually, diatom abundance was highest in 2017 and 2020, while dinoflagellates peaked in 2016 and 2012. Diatoms exhibited a shifting spatial distribution, concentrating in the central and eastern areas during different seasons, while dinoflagellates showed relatively uniform distributions initially, later concentrating in central and northern regions. The spatial patterns were influenced by both seasonal changes and ENSO events. The GAM analysis revealed that diatom abundance was significantly affected by temperature and nutrient availability across different seasons. Under strong El Niño conditions, diatom abundance increased, while dinoflagellate populations remained stable, indicating a competitive advantage for diatoms during these periods. Conversely, dinoflagellate abundance showed no significant variation across interannual events. Our findings underscore the importance of resource availability in shaping phytoplankton communities. Changes in diatoms and dinoflagellate abundance are key indicators of health and stress within the marine ranching ecosystem. This research supports effective management strategies and highlights the need for adaptive practices to enhance coastal engineered ecosystem resilience amid climate change.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"280 ","pages":"Article 121869"},"PeriodicalIF":7.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short and long-term exposure to ocean acidification in limpets from the Castello Aragonese vent systems (Ischia Island, Italy)","authors":"Silvia Giorgia Signorini ,&nbsp;Marco Munari ,&nbsp;Fabio Crocetta ,&nbsp;Isabella Moro ,&nbsp;Ilaria D'Aniello ,&nbsp;Lara Nigro ,&nbsp;Fiorenza Micheli ,&nbsp;Camilla Della Torre","doi":"10.1016/j.envres.2025.121874","DOIUrl":"10.1016/j.envres.2025.121874","url":null,"abstract":"<div><div>Ocean acidification (OA) is reported to entail a detrimental impact on calcifying organisms. Nevertheless, patellid limpets – <em>P. caerulea</em>, <em>P. rustica</em>, and <em>P. ulyssiponensis</em> – are able to persist in extremely low pH conditions inside the Castello Aragonese CO₂ vent systems (Ischia Island), suggesting that they may have developed tolerance to OA, through plasticity and/or adaptive mechanisms. The aim of this study is to evaluate the long-term strategies adopted by limpets that spent their entire life cycle in naturally acidified conditions and the short-term ones induced by a 30-day <em>in situ</em> transplant experiment.</div><div>Regarding native limpet populations, <em>P. caerulea</em> exhibited increasing size and higher energy resources in the extremely acidified site, potentially related to different food availability or to reduction in competition and/or predatory pressure; furthermore, no effects on oxidative stress, biomineralization and neurotoxicity occurred. Similarly, <em>P. ulyssiponensis</em> didn't exhibit any significant effects among different pH conditions regarding biochemical endpoints. Conversely, <em>P. rustica</em> displayed a significant modulation of almost all biochemical parameters, possibly due to its different position on the rocky shore. The short-term exposure of <em>P. caerulea</em> produced a decrease in protein content and an increase in glycogen content in the extreme acidified site, with an induction of superoxide dismutase and glutathione-S-transferases activities in the intermediate pH site.</div><div>Overall, our study revealed that different species of the same genus may have developed distinct responses to OA and suggested different mechanisms to cope with short and long-term exposure to low pH conditions.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121874"},"PeriodicalIF":7.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomics and metabolomics to explore the varied hepatic toxicity induced by aged- and pristine-microplastics: in vivo and human-originated liver organoids-based in vitro study","authors":"Wei Cheng ,&nbsp;Yifei You ,&nbsp;Hange Chen ,&nbsp;Yue Zhou ,&nbsp;Yan Feng ,&nbsp;Yan Wang","doi":"10.1016/j.envres.2025.121820","DOIUrl":"10.1016/j.envres.2025.121820","url":null,"abstract":"<div><div>Microplastics (MP) have distributed ubiquitously and emerged as a significant health risk to human beings. The adverse effect induced by aged MP at concentrations being equivalent to human internal exposure level, has raised special concern, however, is still unclear. In this study, human embryonic stem cells-derived liver organoids (LOs), a novel three-dimensional in vitro model, were exposed to 75 ng/mL self-made polypropylene (PP) and aged PP (aPP), following UV-photoaging for 0- and 500-h respectively, were subject to transcriptomic and metabolomic analysis individually and jointly, to explore the potential adverse effect of PP and aPP on human liver. The mean size of PP and aPP were 7.60 and 6.91 μm, with rough and irregular surface, and varied carbonyl index (CI) (0.08 and 0.25 respectively), indicating there were distinguished physicochemical properties. Transcriptomic analysis suggested the NADH dehydrogenase at mitochondrial complex and ATP synthesis maybe more sensitive to aPP, rather than PP. Metabolomic analysis enriched KEGG pathways including cysteine (Cys) and methionine metabolism significantly. Collectively, the homocysteine (Hcy) metabolism, were anchored upon integrated analysis. To validate, the changes in NADH dehydrogenase-encoding genes, activities of complexs, mitochondrial membrane potential, Hcy and Cys contents, as well, the cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), were detected both in vitro and in vivo. Finally, increased serum Cys and decreased hepatic Cys were confirmed, without inflammation in the liver. The peripheral Hcy may serve as a potential biomarker for indicating the MP-induced systematic adverse health outcomes, due to the disturbance in the Hcy metabolism in the liver.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"280 ","pages":"Article 121820"},"PeriodicalIF":7.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-step synthesis of nitrogen and phosphorus co-doped biochar and its application in dye removal: synergistic effects of adsorption and peroxymonosulfate activation","authors":"Yixuan Shi ,&nbsp;Mingfeng Yin ,&nbsp;Dong Liu,&nbsp;Xingrui Gao,&nbsp;Xiao Liu,&nbsp;Tengteng Yang,&nbsp;Zuoping Zhao,&nbsp;Xiaohui Ji,&nbsp;Caibin Zhao,&nbsp;Xianzhao Shao","doi":"10.1016/j.envres.2025.121866","DOIUrl":"10.1016/j.envres.2025.121866","url":null,"abstract":"<div><div>In the field of advanced oxidation processes (AOPs), the development of catalysts with environmental friendliness and economic benefits faces multiple difficulties, mainly reflected in the catalytic efficiency, selection specificity, and complexity of the synthesis process. This study, we reported a nitrogen and phosphorus co-doped carbon catalyst (CANP800-1) synthesized by a one-step pyrolysis method. The co-doped catalyst was able to achieve 100 % removal of Acid Orange 7 (AO7) in about 30 min and had a high apparent rate constant (<em>k</em>_<em>obs</em> = 0.125 min⁻¹), which is better than unmodified carbon and other single-doped comparative materials. Structural analyses pinpointed that N, P co-doped enhanced specific surface area (1179 m²/g), introduced abundant mesopores, and created a wealth of active sites (such as graphitic nitrogen, C-P bonds) synergistically promoting adsorption and peroxymonosulfate (PMS) activation. The CANP800-1/PMS system had significant adaptability to various water matrices, including pH, coexisting ions, natural organic matter, and real water conditions. A mechanistic investigation confirmed that singlet oxygen (¹O₂) was essential to the reaction process, while electrochemical studies and DFT simulations validated that N/P-induced enhancement of electron transfer and PMS adsorption took place. This study established an innovative metal-free catalytic system that exhibited remarkable effectiveness in sustainable water treatment, providing distinctive solutions and a theoretical basis for ongoing technical difficulties in industrial wastewater treatment.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121866"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging and legacy per- and polyfluoroalkyl substances from offshore oilfields and receiving water in China.","authors":"Yumeng Wang, Hong Chen, Qinghui Xing, Xuemei Xu","doi":"10.1016/j.envres.2025.121865","DOIUrl":"https://doi.org/10.1016/j.envres.2025.121865","url":null,"abstract":"<p><p>Per- and polyfluoroalkyl substances (PFASs) are extensively utilized as oilfield production chemicals and aqueous film-forming foams (AFFFs) in oilfields. A comprehensive investigation was undertaken to analyze twenty per-and polyfluoroalkyl substances (PFASs), including three emerging PFASs in drill cuttings, slurry and produced water from offshore oilfields in three main sea areas of China. The investigation results were further compared with those in their receiving water. The concentration ranges of ΣPFASs in drill cuttings, slurry as well as produced water were 1049-3473 ng/g and 81.9 ng/L-2090 ng/L, respectively. In comparison, the concentrations range of PFASs in receiving water was 46.2-99.7 ng/L. Both sodium p-perfluorous nonenoxybenzenesulfonate (OBS) and hexafluoropropylene oxide dimer acid (HFPO-DA) were identified as the predominant PFASs detected at elevated concentrations in drilling cuttings, slurry, and produced water, demonstrating their extensive utilization in such environments. HFPO-DA and OBS concentrations in produced water exceeded those in receiving water by 1-2 orders of magnitude. Principal component analysis (PCA) analyses revealed that the compositions of PFASs in the receiving water samples exhibited significant similarity to those in drill cuttings, slurry and produced water from oilfields. It was indicated that discharges from oilfields were the primary contributors of PFASs in their receiving water. In 60-96% of samples from produced water in the Bohai Sea and South China Sea oilfields, as well as receiving water adjacent to the Bohai Sea oilfields, the risk quotient (RQ) of HFPO-DA ranged 0.1-1, indicating moderate ecological risks to aquatic organisms. In contrast, legacy PFASs generally showed lower risk levels.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"121865"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomass-based carbon material for next-generation sodium-ion batteries: insights and SWOT evaluation","authors":"Amritesh Kumar ,&nbsp;Nidhi Arora ,&nbsp;Shivam Rawat ,&nbsp;Rakesh K. Mishra ,&nbsp;Aparna Deshpande ,&nbsp;Srinivas Hotha ,&nbsp;Thallada Bhaskar","doi":"10.1016/j.envres.2025.121854","DOIUrl":"10.1016/j.envres.2025.121854","url":null,"abstract":"<div><div>In recent years, energy storage has significantly transitioned from lithium to sodium ion due to sodium's abundance and economical and optimal redox potential. Biomass-based carbon anode materials are extensively studied in sodium-ion batteries because of their economic advantages and eco-sustainable approach. Their distinctive microstructural characteristics resulting in higher specific capacitance. The present review explores hard carbon and a few emerging sustainable materials derived from various biomass sources. It also covers their production, focusing on micro-structures, morphological defects, and heteroatom doping-related aspects. However, the sodium storage mechanism within carbon anodes, particularly hard carbon, is a subject of debate due to its diverse microstructural states in contrast to the specific layered structure of graphite. It also integrates strengths, weaknesses, and opportunities with threat evaluation by highlighting detailed insights about recent developments in hard carbon. This review also highlights bibliographic analysis through network visualization map of international research collaboration in the field of biomass based anode material for sodium ion battery. It also offers a cohesive framework for advancing biomass-derived hard carbon and other carbon materials as an independent or complementary anode material for next-generation sodium-ion batteries.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121854"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constraining the composition and biochemical activity of organic carbon in a large eutrophic estuary using size-fractionated analysis","authors":"Zongqing Lv ,&nbsp;Xiaotian Liu ,&nbsp;Ding He ,&nbsp;Xiangbin Ran ,&nbsp;Yao Feng ,&nbsp;Wenxuan Gao ,&nbsp;Xiaosong Zhong ,&nbsp;Nianzhi Jiao","doi":"10.1016/j.envres.2025.121853","DOIUrl":"10.1016/j.envres.2025.121853","url":null,"abstract":"<div><div>The presence of refractory dissolved organic carbon (RDOC) can result in the misestimation of organic pollution, and documentation regarding the characteristics of organic carbon (OC) and its relationship with pollution is limited. This study employed physical separation, biological incubation, and chemical analysis to examine the size-fractionated composition and bioavailability of OC in the Yangtze River Estuary, one of the most polluted estuarine areas in China. Results revealed that OC chemical features were highly diverse, with RDOC constituting approximately 65.8% ± 9.2% of dissolved organic carbon (DOC). During incubation, less than 10% of CHO molecules (molecules composed solely of carbon, hydrogen and oxygen atoms) identified by ultra-high resolution mass spectrometry were degraded. A significant positive linear relationship between OC and RDOC in size-fractionated OC indicated greater recalcitrance in smaller size fractions. The OC present in the &gt;0.45 μm fraction was notably important for labile OC, including the particulate fraction of OC, which is relevant to chemical oxygen demand (COD) assessments. Excluding RDOC allows for a more accurate estimation of the contribution of labile OC to COD, as represented by the equation: COD_Labile = 0.47 × COD_Bulk - 0.03. Approximately 0.44 ± 0.10 Gt of refractory OC, including 0.31 ± 0.07 Gt of RDOC, is transported annually into the ocean via rivers. This linear relationship of COD reveals an overestimation in current assessments of organic pollution and a neglect of RDOC's role in carbon preservation, thereby necessitating a revision of the COD evaluation practices in estuaries. This study highlights the differentiated impacts of refractory and labile OC on the quantification of OC pollution in a large eutrophic estuary.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121853"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of submerged and air liquid interface exposure: limitations and differences in the toxicological effects evaluated in bronchial epithelial cells","authors":"Y. Allouche ,&nbsp;S. Marchetti ,&nbsp;R. Bengalli ,&nbsp;G. Motta ,&nbsp;L. Pagliarulo ,&nbsp;F. Cazier ,&nbsp;S. Achard ,&nbsp;M. Fadel ,&nbsp;P. Mantecca ,&nbsp;D. Courcot ,&nbsp;F. Ledoux ,&nbsp;A. Verdin ,&nbsp;M. Gualtieri","doi":"10.1016/j.envres.2025.121856","DOIUrl":"10.1016/j.envres.2025.121856","url":null,"abstract":"<div><div>Airborne particulate matter (PM) is the primary environmental cause of death worldwide. The understanding of the toxicological mechanism has improved over the last decades, nonetheless now novel exposure systems allow the possibility to mimic the <em>in vivo</em> interaction between particles or particle constituents and lung cells. Here we report the results obtained after exposure to the organic extract of fine PM (PM_2.5-0.3) of the BEAS-2B cells cultured in submerged or at the air liquid interface (ALI) conditions. Deposition efficiency of both PM and its organic extract was followed. Cell viability, inflammatory responses and gene expression were then evaluated. The comparison of submerged vs ALI exposure revealed significant differences in the expression of specific genes such as <em>CYP1A1</em> and <em>NQO1</em> 6h after exposure and <em>CYP1B1</em> and <em>NFkB1</em> 24h post exposure. Also, the results demonstrate the importance of evaluating the efficiency of deposition of the treatment to avoid misinterpretation of the results. PM particles determined an extremely low deposition efficiency, making it impossible to use this particle for ALI exposure tests. Although biological results, such as gene expression, may vary between the two exposure approaches (submerged or ALI), the ALI exposure should be preferred as it is more representative of human exposure via the respiratory route.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121856"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bicarbonate on electro-bioremediation of phenanthrene-contaminated groundwater","authors":"Xin Zhang,&nbsp;Yue Liu,&nbsp;Rongrong Liu,&nbsp;Yiting Qiu,&nbsp;Bohan Zhang,&nbsp;Nannan Zhao,&nbsp;Ruo He","doi":"10.1016/j.envres.2025.121859","DOIUrl":"10.1016/j.envres.2025.121859","url":null,"abstract":"<div><div>Electro-bioremediation under anaerobic conditions is an effective approach for refractory organic matter removal in groundwater. Bicarbonate (HCO₃⁻) is an inorganic carbon source and electron acceptor in groundwater, however, the influencing mechanism of HCO₃⁻ on pollutant removal of electro-bioremediation remains unclear. Herein, the effects of HCO₃⁻ concentration on electro-bioremediation of phenanthrene (PHE)-contaminated groundwater were investigated. HCO₃⁻ could facilitate the PHE degradation while an HCO₃⁻ concentration of higher than 1000 mg L⁻¹ had a significant inhibition effect. Among the HCO₃⁻ concentration of 100–5000 mg L⁻¹, the highest PHE degradation efficiency of 75.04–80.18 % was achieved in the electro-biochemical reactor with 500 mg L⁻¹ HCO₃⁻. The PHE removal efficiency was negatively correlated with the current density during the electro-bioremediation process, due to the effect of HCO₃⁻ concentrations on the electrolyte conductivity in the reactors. The electro-bioremediation process could increase the richness of diversity of microbes. <em>Methanomethylovorans</em> and the PHE-degrading bacteria including <em>Pelolinea</em>, <em>Clostridium sensu stricto</em> 5, <em>Diaphorobacter, Methyloversatilis</em> and <em>Flavobacterium</em> were the main microbes involved in PHE degradation. Of them, <em>Methanomethylovorans</em> was significantly positively correlated with the PHE removal efficiency. The potential metabolic function analysis revealed that the bacterial chemotaxis, flagellar assembly, carbohydrate metabolism and ABC transporters were prompted with the addition of HCO₃⁻, while they were inhibited with the increasing HCO₃⁻ concentration. These findings suggested that electro-bioremediation technology was suitable for the remediation of polycyclic aromatic hydrocarbons such as PHE-contaminated groundwater in low bicarbonate areas.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121859"},"PeriodicalIF":7.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}