Environmental Research最新文献

{"title":"Independent impacts of co-formulants contained in anthelmintic formulations on seed germination of grassland species","authors":"Carsten Eichberg ,&nbsp;Lars Laber ,&nbsp;Scarlett Jaß ,&nbsp;Andrés García ,&nbsp;Tobias W. Donath","doi":"10.1016/j.envres.2026.123762","DOIUrl":"10.1016/j.envres.2026.123762","url":null,"abstract":"<div><div>Agrochemicals have been shown to affect non-target organisms and are often applied as mixtures of active ingredient and co-formulants. Although there is evidence that co-formulants play a role in these effects, their contribution has hardly been studied.</div><div>Therefore, we studied effects of co-formulants on seed germination (percentage, time, synchrony) using a common anthelmintic formulation. The seeds of four species of temperate grasslands (<em>Achillea ptarmica</em>, <em>Agrostis capillaris</em>, <em>Dianthus deltoides</em>, <em>Plantago lanceolata</em>) were exposed to a commercial moxidectin formulation at a concentration of 10 mg l⁻¹ as well as three of its co-formulants: benzyl alcohol (BA), butylated hydroxytoluene (BHT) and disodium ethylenediamine tetraacetic acid (EDTA-Na₂).</div><div>Results showed that the tested anthelmintic formulation as a whole and two of its co-formulants (BA, BHT) as individual substances significantly impacted germination behaviour of the test species. Formulation, BA and BHT significantly reduced germination percentage in all species compared to the control, with BA exerting the strongest effect (reduction up to 99 %). The same three treatments significantly increased mean germination time in all species by a factor of two to four. Germination synchrony showed a weaker response and was only affected in two species (<em>A. capillaris</em>, <em>D. deltoides</em>) and only by BHT (reduction of 49 % and 60 %, respectively).</div><div>The strong effects of BA and BHT on seed germination suggest that their contribution to effects of anthelmintic formulations is at least partly due to an independent impact. Therefore, co-formulants should be given greater consideration both in the design of ecotoxicological experiments and in environmental risk assessments.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123762"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical exercise mitigates the adverse effects of PM2.5 constituents on lung function: New evidence for inflammatory pathways from a crossover study","authors":"Pengpeng Wu ,&nbsp;Qian Guo ,&nbsp;Ling Qi ,&nbsp;Yuchen Zhao ,&nbsp;Mengyao Bian ,&nbsp;Chen Wang ,&nbsp;Bin Han ,&nbsp;Junfeng (Jim) Zhang ,&nbsp;Xiaoli Duan","doi":"10.1016/j.envres.2026.123743","DOIUrl":"10.1016/j.envres.2026.123743","url":null,"abstract":"<div><div>Physical exercise in polluted air involves a significant trade-off. However, the interaction effects and underlying mechanisms between PM_2.5 constituents and physical exercise on lung function remain poorly understood. This crossover study was conducted among 74 adults who completed a session of outdoor physical exercise and a session of no exercise in random order. We analyzed the chemical constituents of PM_2.5 that the subjects were exposed to during each session, measured lung function, and assessed 10 biomarkers of systemic inflammation both before and after each session. We used linear mixed-effect models to assess the independent and interactive effects of PM_2.5 constituents and physical exercise on lung function and biomarkers. We also conducted a moderated mediation analysis to examine the role of inflammatory factors that mediated the effects of PM_2.5 constituents and physical exercise on lung function. We found that traffic emissions and resuspended dust may be major contributors to the adverse effects of PM_2.5 on lung function, and that physical exercise significantly attenuated these detrimental impacts. We also identified heat shock protein 70 (HSP70), granulocyte-macrophage colony stimulating factor, toll-like receptor 4 protein (TLR-4), Clara cell protein 16, and interleukin-8 as the mediators in the association. Our results support the hypothesis that PM_2.5 constituents and physical exercise interact to affect lung function through the HSP70-mediated regulation of the TLR4/NF-κB inflammatory pathway. These findings are expected to inform future control strategies targeting source-specific PM_2.5 constituents and public health decisions on exercise on air pollution days.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123743"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-driven water quality index prediction in the Dau Tieng reservoir, southern Vietnam, with interpretability via SHapley additive exPlanations: A vision for water quality management strategies","authors":"Thanh Tung Nguyen ,&nbsp;Trung Hieu Nguyen ,&nbsp;Thuy Vy Tran ,&nbsp;Nguyen Tuan Anh ,&nbsp;Minh-Ky Nguyen ,&nbsp;Thai Van Anh ,&nbsp;Wen-Pei Tsai ,&nbsp;Hoang Huy Huynh","doi":"10.1016/j.envres.2026.123714","DOIUrl":"10.1016/j.envres.2026.123714","url":null,"abstract":"<div><div>Freshwater reservoirs are essential for ecological stability, biodiversity preservation, and resource sustainability. Managing water quality effectively poses challenges due to complex environmental pressures and limitations in traditional monitoring methods. This study utilized machine learning (ML) algorithms along with SHapley Additive exPlanations (SHAP) to predict the Water Quality Index (WQI) of the Dau Tieng reservoir in southern Vietnam. A total of 160 water samples were collected from 20 locations across eight sampling campaigns (October 2022–April 2024). Key physicochemical parameters included pH, dissolved oxygen (DO), alkalinity (Alk), total suspended solids (TSS), total ammonium nitrogen (TAN), nitrite (NO₂⁻-N), phosphate (PO₄^3--P), and chlorophyll-a (Chl-a). Concentrations ranged from TSS: 2.7–43 mg/L, Chl-a: 3.3–185.3 mg/m³, TAN: 0.01–0.125 mg/L, NO₂⁻-N: 0.005–0.072 mg/L, and PO₄^3--P: 0.012–0.465 mg/L. Approximately 35 % of TSS, 38.8 % of Alk, 21.3 % of DO, and 15 % of Chl-a samples exceeded QCVN/WHO permissible limits, especially during the rainy season. Among the models, the Random Forest (RF) algorithm achieved the highest performance with R² = 0.91, Root Mean Squared Error (RMSE) = 6.086, and Mean Absolute Error (MAE) = 4.058 on the testing dataset, and model validation employed a train/test split (80/20) to assess generalization performance. SHAP analysis revealed that TSS, pH, DO, and Chl-a were the most influential predictors of WQI, indicating that sediment-driven turbidity and nutrient enrichment are key drivers of water quality degradation. The integrated Principal Component Analysis (PCA)–ML–SHAP framework not only enhances predictive accuracy but also provides interpretable insights to inform sustainable water governance and biodiversity management in tropical reservoir systems.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123714"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in cardiovascular biomarkers and gut microbiome associated with night shift work: Insights from the Chinese platform workers study","authors":"Tenglong Yan ,&nbsp;Li Rong ,&nbsp;Siyuan Wang ,&nbsp;Xin Song ,&nbsp;Binshuo Hu ,&nbsp;Zhihui Wang ,&nbsp;Xiaoshun Wang ,&nbsp;Xiaowen Ding ,&nbsp;Rui Guan ,&nbsp;Hong Yu ,&nbsp;Dongsheng Niu ,&nbsp;Ying Zhang","doi":"10.1016/j.envres.2026.123735","DOIUrl":"10.1016/j.envres.2026.123735","url":null,"abstract":"<div><div>The expansion of the gig economy has been marked by an increase in platform workers, including ride-hailing drivers, who typically work non-standard schedules, with night shifts being common. Although night shift work was known to disrupt circadian rhythms and elevate cardiovascular risks, the interplay between early alteration in cardiovascular biomarkers and gut microbiome remain unclear. To investigate these associations in the platform worker groups, we conducted a cross-sectional study comparing 66 night shift and 175 day shift ride-hailing drivers in Beijing, China. All participants underwent health examinations, provided blood tests for the assessment of four key cardiovascular biomarkers (hs-cTnI, ET-1, NT-pro-BNP, and FABP-3), and submitted fecal samples for gut microbiome profiling via 16S rDNA sequencing. Bioinformatics, linear regression, and mediation analyses were conducted to evaluate the associations between night shift work, biomarkers, and microbial taxa, with adjustments for key sociodemographic and lifestyle factors. Night shift drivers exhibited significantly higher levels of NT-pro-BNP (median 49.8 vs. 41.3 ng/L, <em>P</em> = 0.044), the association that remained significant after adjustment for covariates (<em>β</em> = 0.195, <em>P</em> = 0.004). Twenty differentially abundant taxa were identified, four of which were significantly associated with hs-cTnI. This study found that night shift work in ride-hailing drivers was significantly associated with adverse cardiovascular biomarker profiles and distinct gut microbiome alterations. Correlation analysis further suggested that gut microbial changes were linked to the observed cardiovascular risk. This study reported associations between night-shift work and alterations in early cardiovascular injury biomarkers as well as the gut microbiota, suggesting a potential link between gut microbiota and night-shift-related cardiovascular injury.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123735"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycyclic aromatic hydrocarbons in PM2.5 and PM10 in Lagos, Nigeria: Sources, characteristics, and health risks","authors":"Adebola A. Odu-Onikosi ,&nbsp;Paul A. Solomon ,&nbsp;Philip K. Hopke ,&nbsp;Pierre Herckes ,&nbsp;Matthew Fraser","doi":"10.1016/j.envres.2026.123745","DOIUrl":"10.1016/j.envres.2026.123745","url":null,"abstract":"<div><div>Particulate matter (PM) bound polycyclic aromatic hydrocarbons (PAHs) were assessed across Lagos, Nigeria for their spatial-temporal variations, sources, and health risks. PM samples were collected with low-volume samplers at six sites during 12 months and analyzed by GC–MS for 16 PAHs. Sources were identified using Positive Matrix Factorization (PMF), and health risks were assessed using the United States Environmental Protection Agency's Incremental Lifetime Cancer Risk (ILCR) methodology with site-appropriate exposure times following ATSDR guidelines. Annual mean concentrations of the sum of 16 PAHs (Σ16PAH) were 44.9 ± 38.5 ng/m³ in PM_2.5 and 50.3 ± 42.6 ng/m³ in PM₁₀ across Lagos. Dry season values (PM_2.5 PAHs: 76.2 ng/m³, PM₁₀ PAHs: 85.7 ng/m³) were &gt;3 times the wet season values (21.9 and 24.1 ng/m³, respectively). These concentrations are higher than in many cities but comparable to and generally lower than the most polluted megacities. Industrial and port-adjacent sites consistently recorded the highest levels, while suburban/coastal sites were the lowest. PMF identified factors for PM_2.5 as diesel (18%), gasoline (15%), petroleum handling (14%), industrial (14%), marine (14%), biomass burning (13%), and background (12%). For PM₁₀, factors include biomass burning (21%), background (20%), petroleum (15%), diesel (14%), industrial (14%), gasoline (8%), and marine (7%). Most of the Σ16PAH (89%) occurred in PM_2.5. Meteorological factors, particularly temperature influenced PAH concentrations by affecting the gas-particle partitioning of semi-volatile PAHs, dispersion, and removal processes, with particle-bound concentrations typically higher in cooler periods. Health risk assessments estimated ILCRs of 2.0 to 6.1 × 10⁻⁶, exceeding the USEPA benchmark (1 × 10⁻⁶) by 2–6 times at all sites. The corresponding loss of life expectancy ranged from 12 to 38 min over a 30-year exposure period with residential and industrial zones most affected. These results highlight the need for targeted emission controls and health-focused policies in Lagos.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123745"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Efficient biogenesis of calcium oxide nanoparticles using the extract of Eleusine coracana seeds and their application against multidrug-resistant ocular bacterial pathogens” [Environ. Res. 251 (2024) 118632]","authors":"Sandhanasamy Devanesan ,&nbsp;Heber A. David ,&nbsp;Amirtham J. Ranjitsingh ,&nbsp;Tariq Alzahim ,&nbsp;Robert Selvam ,&nbsp;Mohamad S. AlSalhi","doi":"10.1016/j.envres.2026.123782","DOIUrl":"10.1016/j.envres.2026.123782","url":null,"abstract":"","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123782"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146027964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mercury and other trace elements in gull chicks from a highly urbanised environment: effects on chick growth, physiology, and health condition","authors":"Ivo dos Santos ,&nbsp;Vitor H. Paiva ,&nbsp;Ana C. Norte ,&nbsp;Carine Churlaud ,&nbsp;Maud Brault-Favrou ,&nbsp;Joana Pais de Faria ,&nbsp;Nathalie Almeida ,&nbsp;Ricardo Fernandes ,&nbsp;Jaime A. Ramos ,&nbsp;Paco Bustamante","doi":"10.1016/j.envres.2026.123701","DOIUrl":"10.1016/j.envres.2026.123701","url":null,"abstract":"<div><div>Coastal urbanisation has increased the exposure of urban-dwelling organisms to contaminants, such as trace elements (TEs) in opportunistic seabirds. This exposure may cause detrimental health effects, especially during sensitive early life-stages, before detoxification mechanisms are fully developed. We monitored yellow-legged gull (<em>Larus michahellis</em>) chicks in the city of Porto, Portugal, and collected down feathers and blood for mercury (Hg) and other TE analyses. We aimed to i) evaluate the effects of maternal exposure to Hg (inferred from down feathers) on early chick development, and ii) examine the impact of blood TE contamination on chick physiology and health condition. Aligned with previous research, blood Hg decreased as chicks aged (up to the 5^th week), due to the growth dilution effect and depuration into the growing feathers. Additionally, down feather Hg concentrations showed a limited transfer of Hg from females to the embryo and was unrelated to hatching mass or early growth rate. While low maternal Hg exposure resulted in no detectable effects on early-life development or chick survival, dietary exposure (inferred from blood) to other TEs incurred physiological costs. Blood arsenic (As) and lead (Pb) concentrations were associated with a faster erythrocyte sedimentation rate, suggesting impaired immune response and inflammation. This highlights a dichotomy between transgenerational Hg transfer and post-hatching dietary exposure to Hg and other TEs. Future work should aim to define toxicity thresholds for TEs in seabird chicks, investigating the implications in the health since early life-stages, to better integrate the observed physiological responses.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123701"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of graphite powder/PDMS synergistically modified melamine sponge and its application for photothermal crude oil removal","authors":"Jian Wu ,&nbsp;Yingying Wang ,&nbsp;Jingjing He ,&nbsp;Taipeng Jiang ,&nbsp;Weijie Wei ,&nbsp;Lingling Zeng ,&nbsp;Sheng Chen ,&nbsp;Zhan Du ,&nbsp;Jin Li ,&nbsp;Zhuwei Gao","doi":"10.1016/j.envres.2025.123474","DOIUrl":"10.1016/j.envres.2025.123474","url":null,"abstract":"<div><div>The massive discharge of industrial oily wastewater and frequent marine oil spills pose a serious threat to ecosystems and public health. In this study, a cost-effective graphite powder/polydimethylsiloxane-modified melamine sponge (MF-PDMS-GP) was fabricated by a facile impregnation-curing method for photothermal-assisted crude oil removal. By synergistically integrating micro/nano permeable surfaces and low surface energy coatings, the composite achieved hydrophobicity (water contact angle of 140.7°). This material exhibited selective adsorption capacity for both light and heavy oils in oil-water mixtures. Additionally, it possessed excellent self-cleaning capability and chemical stability. Thanks to the exceptional light and heat properties imparted by graphite powder, the material reached a surface temperature of 78.3 °C in just 2 min under simulated sunlight (1 kW/m²). This study provided a simple, low-cost, and environmentally friendly method for the adsorption and separation of highly viscous oil spills at sea.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123474"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145675941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure trends of neonicotinoids from 2008 to 2018 and health risk in a longitudinal study in China","authors":"Jiazhen Zhang ,&nbsp;Hong Guo ,&nbsp;Lin Liu ,&nbsp;Jinglan Ma ,&nbsp;Jiao Zou ,&nbsp;Zhengyang Xiao ,&nbsp;Zhewen Li ,&nbsp;Yunqi Luo ,&nbsp;Caizheng Yu ,&nbsp;Meian He","doi":"10.1016/j.envres.2026.123820","DOIUrl":"10.1016/j.envres.2026.123820","url":null,"abstract":"<div><h3>Background</h3><div>Neonicotinoids (NNIs) are one of the most widely used insecticides in the world, yet researches on the exposure trends and health risks of NNIs are still limited.</div></div><div><h3>Objectives</h3><div>To explore the decadal exposure trend and health risk of NNIs in a longitudinal study based on the Dongfeng-Tongji cohort.</div></div><div><h3>Methods</h3><div>Participants in the present study (a total of 216 individuals) were drawn from the Dongfeng-Tongji cohort. We measured serum levels of 10 neonicotinoid parent compounds and 3 metabolites at three time points (2008, 2013, and 2018). Linear mixed effect models were used to investigate trends in NNIs concentrations over time. Hazard quotients (HQs) were calculated based on NNIs exposure levels to assess health risks. Latent class trajectory analysis was employed to identify distinct exposure trajectories for NNIs.</div></div><div><h3>Results</h3><div>The detection rates and exposure levels of NNIs showed a significant increase over decade (all <em>P</em> &lt; 0.001). Only IMI and N-ACE achieved a detection rate exceeding 65 % at each survey, and N-ACE was the predominant NNI. HQs were all well below 1, but showed a significant upward trend over time (all <em>P</em> &lt; 0.05). The trajectories of IMI could be divided into four groups, with age and body mass index potentially influencing grouping.</div></div><div><h3>Conclusions</h3><div>This study explored time trend of NNIs and associated health risks. It provided crucial scientific evidence for protecting environmental and public health, as well as for formulating health policies.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"293 ","pages":"Article 123820"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering immobilized microbes with surface-displayed cold-adapted nitroreductase: An efficient strategy for nitrobenzene degradation at low temperature","authors":"Yanhua Hou,&nbsp;Yujie Li,&nbsp;Yifan Zou,&nbsp;Yatong Wang,&nbsp;Quanfu Wang","doi":"10.1016/j.envres.2026.123985","DOIUrl":"10.1016/j.envres.2026.123985","url":null,"abstract":"<div><div>Nitrobenzene (NB), a typical example of aromatic nitro compounds, has become a significant pollutant because its wastewater is difficult to degrade in low-temperature environments. This study constructed an engineered strain displaying a cold-adapted nitroreductase (PsNTR) for enhanced low-temperature degradation efficiency, employing a cell surface display system in which the N-terminal domain of the ice nucleation protein (INPN) served as the anchoring carrier. SDS-PAGE, Western blot analysis, and protease accessibility assays confirmed that the INPN-PsNTR fusion protein (55.40 kDa) was stably expressed and displayed on the surface of <em>Escherichia coli</em>. The engineered strain BL21/INPN-PsNTR maintained outer membrane integrity and exhibited robust growth during the display process. To further enhance the storage stability and reusability of the engineered strain for practical applications, it was immobilized using modified diatomite (DA-PEI/GA). Notably, PsNTR@DA-PEI/GA demonstrated high efficiency in degrading NB at low temperatures, with a degradation rate of 92.75% for 500 mg/L NB within 120 h at 15 °C. In summary, this study employed PsNTR surface display technology as the core strategy, combined with a cell immobilization approach, to establish a novel and promising technological pathway for efficient and green degradation of NB at low temperatures, thereby offering a new strategy for the remediation of NB contamination.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"295 ","pages":"Article 123985"},"PeriodicalIF":7.7,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}