Environmental Research最新文献

{"title":"Pyrolysis oil to power: Carbon nanomaterials as photosensitizers for high-performance Dye-Sensitized Solar Cells","authors":"Kyaw Ye Aung,&nbsp;Qingbo Li,&nbsp;Lei Li,&nbsp;Yidan Wang,&nbsp;Wanning Ren","doi":"10.1016/j.envres.2025.121689","DOIUrl":"10.1016/j.envres.2025.121689","url":null,"abstract":"<div><div>Pyrolysis oil, a byproduct of biomass pyrolysis, has faced significant challenges due to its high acidity, complex composition, and low heating value, limiting its direct application. This study presents an innovative approach to valorize cornstalk-derived pyrolysis oil by transforming it into advanced carbon-based nanomaterials for Dye-Sensitized Solar cells (DSSCs). Through atmospheric distillation, pyrolysis oil was converted into bio-oil-derived char, featuring a hybrid structure of graphitic (sp²) and disordered (sp³) domains enriched with oxygen and nitrogen functionalities. This char served as a sustainable precursor for nanocarbon production (BCDs). Two types of carbon nanomaterials were synthesized: Carbon Quantum Dots via N, N-dimethylformamide (DMF) solvothermal treatment, and Carbon Nanodots via hydrogen peroxide (H₂O₂) oxidation. BCDs (DMF) exhibited a crystalline graphitic structure, green fluorescence, a high quantum yield (19.9 %), and a narrow band gap (2.2 eV). In contrast, BCDs (H₂O₂) displayed an amorphous structure, blue fluorescence, a lower quantum yield (9 %), and a wider band gap (2.8 eV). When applied as photosensitizers in DSSCs, BCDs (DMF) achieved a remarkable 59.2 % increase in power conversion efficiency (PCE) and a 47.7 % enhancement in short-circuit current density (Jsc) compared to BCDs(H₂O₂). The superior performance of BCDs (DMF) is attributed to their enhanced light absorption, lower recombination rates (k_eff), extended electron lifetimes (τ_n), faster electron transport times (τ_s), and more efficient charge transfer (R_ct). This study demonstrates a sustainable strategy for converting pyrolysis oil into functional nanomaterials, unlocking new opportunities for biomass-based clean energy applications.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121689"},"PeriodicalIF":7.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast adsorption of low-concentration ammonia nitrogen by persulfate-modified carbon materials: structure influence, performance, and mechanism","authors":"Aijuan Zhou ,&nbsp;Liangxia Zhu ,&nbsp;Yuting Chen ,&nbsp;Jingwen Wang ,&nbsp;Yong Liu","doi":"10.1016/j.envres.2025.121680","DOIUrl":"10.1016/j.envres.2025.121680","url":null,"abstract":"<div><div>Carbon materials (CMs) have emerged as cost-effective adsorbents with significant potential for environmental remediation, yet their limited adsorption performance for ammonia nitrogen (NH₄⁺-N) restricts their application in water purification. Herein, persulfate (PS)-modified carbon nanotubes (MCNTs) were synthesized to enhance NH₄⁺-N adsorption from aqueous solutions. The MCNTs showed a well-developed mesoporous structure, higher graphitization degree, and more carbonyl (C=O) groups than pristine carbon nanotubes (CNTs). These structural modifications resulted in a remarkable adsorption performance, achieving both rapid adsorption equilibrium (≤3 min) and a 2- to 3-fold increase in adsorption capacity (compared to pristine CNTs). The adsorption rate constant of MCNTs was 2.58 mg/(g∗min) and the maximum adsorption capacity reached 15.49 mg/g. Mechanistic studies revealed that the enhanced mesoporous ratio of MCNTs significantly accelerated adsorption kinetics, whereas high graphitization degree and elevated C=O content contributed to their superior adsorption capacity and rate. Additionally, saturated MCNTs were effectively regenerated using a sodium hydroxide solution and maintained stable adsorption performance after 5-cycles. This modification strategy demonstrated excellent universal applicability across various CMs. To demonstrate practical applicability, the modified CMs (MCMs) were loaded on an ultrafiltration membrane and employed in a continuous flow adsorption system for treating aquaculture wastewater. The system reduced the NH₄⁺-N concentration in the effluent to below 0.3 mg/L within 80 min. This work not only provides a novel class of high-performance MCMs for NH₄⁺-N removal from wastewater but also elucidates the critical role of CMs' structure properties in governing adsorption performance.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121680"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe-MOFs-derived Fe3O4-doped biochar from waste chopsticks: a novel catalyst for tetrabromobisphenol a degradation via peroxymonosulfate activation","authors":"Van-Anh Thai ,&nbsp;Thanh-Binh Nguyen ,&nbsp;Wei-Hsin Chen ,&nbsp;Chiu-Wen Chen ,&nbsp;Ruey-an Doong ,&nbsp;Cheng-Di Dong","doi":"10.1016/j.envres.2025.121620","DOIUrl":"10.1016/j.envres.2025.121620","url":null,"abstract":"<div><div>Biochar production has emerged as a highly effective strategy for waste-to-resource conversion. In this study, biochar derived from waste chopsticks was doped with Fe₃O₄ using MIL-100 (Fe)—a metal-organic framework (MOF)—was synthesized at various pyrolysis temperatures and designated as Fe₃O₄@BC. The catalyst was employed to activate peroxymonosulfate (PMS) for the removal of tetrabromobisphenol A (TBBPA), a widely used brominated flame retardant (BFR). Remarkably, the Fe₃O₄@BC/PMS system achieved 98 % TBBPA removal within 30 min. The exceptional catalytic performance of Fe₃O₄@BC was attributed to the uniform dispersion of iron oxides and the abundance of oxygen-containing functional groups on the biochar surface. The underlying mechanism of TBBPA degradation was systematically investigated, revealing two distinct degradation pathways and identifying 18 by-products using liquid chromatography-mass spectrometry (LC-MS) and density functional theory (DFT) analysis. Furthermore, scavenger tests and electron paramagnetic resonance (EPR) spectra demonstrated that superoxide radicals (<span><math><mrow><msup><msub><mi>O</mi><mn>2</mn></msub><mrow><mo>·</mo><mo>−</mo></mrow></msup></mrow></math></span>) played a critical role in TBBPA degradation within the catalyst/PMS system. This study highlights the immense potential of biochar derived from waste chopsticks as an eco-friendly and efficient catalyst. Key advantages include the utilization of solid waste, reduced toxicity of degradation intermediates, and effective PMS activation for the degradation of BFRs.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121620"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrified activation of peroxymonosulfate using carbonaceous composite membranes for sulfamethoxazole removal: Treatment efficiency, mechanistic insights, and intermediate toxicity evaluation","authors":"Feiyue Qian ,&nbsp;Yu Yan ,&nbsp;Nian Liu ,&nbsp;Xin Xia ,&nbsp;Ming Gao ,&nbsp;Tingting Liu ,&nbsp;QingJie Xie","doi":"10.1016/j.envres.2025.121678","DOIUrl":"10.1016/j.envres.2025.121678","url":null,"abstract":"<div><div>Metal-free carbonaceous materials can effectively activate peroxymonosulfate (PMS) for organic pollutant degradation by utilizing their surface active sites. In this study, an electrified membrane was fabricated using nitrogen-doped graphene (NG) sheets and carbon nanotubes (N-CNTs) to investigate the impact of different electrified modes on membrane performance for sulfamethoxazole (SMX) removal from water matrices. Characterization results indicated that NG/N-CNT mats exhibited superior electron transfer ability for PMS activation due to their abundant defects and nitrogen-doped species. When used as the cathode (Mode III), the carbon mats achieved a pseudo-first-order kinetic constant (<em>k</em>_obs) of 5.305 s⁻¹ (318.2 min⁻¹) for SMX removal, which was 51.63 % and 24.95 % higher than those in Mode I (no applied potentials) and Mode II (carbon anode), respectively. Reactive oxygen species identification revealed that non-radical pathways govern in-situ catalytic oxidation, with the relative contributions of surface-confined oxidation and singlet oxygenation significantly varying across different electrified modes. In contrast, Mode III significantly enhanced PMS activation, minimized the depletion of active sites (such as defects and pyridinic nitrogen), and reduced the accumulation of oxidation intermediates within the carbon mats. After continuous filtration of 12,000 bed volumes of carbon mats, Mode III still achieved over 85 % SMX removal from real river water while maintaining a high water flux of 116 L m^−2.h^−1.bar⁻¹. Intermediate composition analysis demonstrated that the filtrate from Mode III posed lower risks of acute and developmental toxicity compared to those from the other electrified modes. These findings provide a reliable and enhanced approach for efficient in-situ catalytic oxidation.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121678"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The toxicological effects of perfluorooctanoic acid (PFOA) exposure in large yellow croaker (Larimichthys crocea): exploring the relationship between liver damage and gut microbiota dysbiosis","authors":"Ping Han ,&nbsp;Yadong Xue ,&nbsp;Zhennan Sun ,&nbsp;Xiumei Liu ,&nbsp;Liang Miao ,&nbsp;Mingzhe Yuan ,&nbsp;Xubo Wang","doi":"10.1016/j.envres.2025.121683","DOIUrl":"10.1016/j.envres.2025.121683","url":null,"abstract":"<div><div>Per- and polyfluoroalkyl substances (PFASs) are synthetic organofluorine compounds characterized by their persistence, toxicity, and bioaccumulative properties, rendering them substantial environmental contaminants. However, limited research has investigated the effects of a short-term low-concentration PFAS exposure on the hepatic and intestinal systems of marine fish. In this study, large yellow croaker was selected as the experimental subject to explore the toxic effects of exposure to 1000 ng/L PFOA after 3, 7, and 14 days, with a focus on liver and gut microbiota. The results demonstrated that a short-term exposure to PFOA induced significant histopathological damage in both liver and gut, with cumulative effects becoming more pronounced over time. Moreover, transcriptome analysis of the liver revealed that PFOA exposure significantly altered the expression of genes associated with lipid metabolism, inflammatory response, and cellular apoptosis. GO and KEGG functional enrichment analyses showed significant enrichment in the P53, NF-κB, MAPK, and PPAR signaling pathways. On the other hand, 16S rRNA gene sequencing demonstrated that PFOA exposure resulted in a decline in gut microbiota diversity, an increase in the abundance of potentially pathogenic bacteria (e.g. Proteobacteria), and a significant reduction in beneficial bacteria (<em>Lactobacillus</em>). These changes indicated gut microbiota dysbiosis. Correlation analysis between gut microbiota changes and potential liver damage indicators suggested an association between liver damage and gut microbiota dysbiosis. Furthermore, we propose a hypothetical model involving lipid accumulation-mediated mitochondrial oxidative stress and inflammation pathway activation, triggered by damage-associated molecular patterns (DAMPs) resulting from PFOA exposure. These findings offered valuable insights into the toxic effects of a short-term low-concentration PFOA on the hepatic and intestinal systems of large yellow croaker, and establish a connection between liver damage to gut microbiota dysbiosis after PFOA exposure.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121683"},"PeriodicalIF":7.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achieving partial nitrification and denitrification coupled with simultaneous partial nitrification, anammox, and denitrification (PND-SNAD) by the inhibition of sulfide to accomplish stabilized nitrogen removal","authors":"Yihao Bian ,&nbsp;Kunming Fu ,&nbsp;Ruotong Xu ,&nbsp;Teng Guan ,&nbsp;Aotong Huo ,&nbsp;Ruibao Zhang ,&nbsp;Xueqin Li ,&nbsp;Fuguo Qiu ,&nbsp;Yongji Zhang","doi":"10.1016/j.envres.2025.121630","DOIUrl":"10.1016/j.envres.2025.121630","url":null,"abstract":"<div><div>The simultaneous partial nitrification, anammox, and denitrification (SNAD) process is widely applied for treating high-ammonia wastewater, but its application to low-ammonia organic wastewater has been scarcely explored. In this study, a partial nitrification and denitrification coupled with simultaneous partial nitrification, anammox, and denitrification (PND-SNAD) system was established to treat organic wastewater with low ammonia concentration. Experimental results revealed that sulfide at 5 mg/L selectively inhibited nitrite-oxidizing bacteria (NOB) but had little effect on ammonium-oxidizing bacteria (AOB). Finally, NOB was suppressed in PND system by intermittently adding sulfide to the PND system. The PND system provided nitrite and activated sludge enriched with AOB to the SNAD system during stable operation. The SNAD system demonstrated chemical oxygen demand (COD) and nitrogen removal efficiencies of 89.86 % and 86.45 %. <em>Candidatus Brocadia</em> and <em>Nitrosomonas</em> were the main ammonium oxidizing bacteria (AnAOB) and AOB. The contribution of AOB and denitrifying bacteria (DNB) to nitrogen transformation was 67.15 % and 25.33 % in the PND system. In the SNAD system, the contributions of AnAOB, AOB, and DNB were 34.40 %, 33.59 %, and 27.56 %, respectively. Overall, this study provided a new sustainable strategy for treating organic wastewater with low ammonia concentration.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121630"},"PeriodicalIF":7.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review of sensors for detecting per- and polyfluoroalkyl substances: Focusing on diverse molecular probes","authors":"Jiancheng Zha ,&nbsp;Muyuan Ma ,&nbsp;Yue Shen ,&nbsp;Lei Sun ,&nbsp;Jing Su ,&nbsp;Chong Hu ,&nbsp;Shuai Wang ,&nbsp;Panpan Cui ,&nbsp;Yuan Zhou ,&nbsp;Feng Liu","doi":"10.1016/j.envres.2025.121669","DOIUrl":"10.1016/j.envres.2025.121669","url":null,"abstract":"<div><div>Per and Polyfluoroalkyl Substances (PFASs) pose a severe threat to the ecological environment and human health due to their persistence, bioaccumulation, and potential toxicity in the environment. Currently, the detection methods of PFASs generally rely on the combination of chromatographic techniques and mass spectrometry, which are typically suitable for laboratory testing. To meet the requirements of on-site detection, there is an urgent need to develop convenient and efficient detection methods. Sensors, as the preferred alternative, have been widely studied. In order to deeply investigate the mechanism of sensors in recognizing PFASs, this review, from the unique perspective of molecular probes, summarizes the construction and recognition mechanisms of four molecular probes: antibodies, aptamers, synthesized micromolecules, and synthesized polymers for PFASs. This review focuses on PFOA and PFOS as representative perfluoroalkyl substances and systematically investigates their properties and effects. It also analyzes the respective advantages, disadvantages, and applicable scenarios, and discusses the future development trends.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121669"},"PeriodicalIF":7.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient degradation of antibiotic pollutants in water by Ca2+/Ce3+ Co-doped Bi2O2CO3 photocatalysts","authors":"Yu Zhang,&nbsp;Yangang Sun,&nbsp;Luyao Pan,&nbsp;Zhaoxia Wen,&nbsp;Min Shi,&nbsp;Hao Li","doi":"10.1016/j.envres.2025.121668","DOIUrl":"10.1016/j.envres.2025.121668","url":null,"abstract":"<div><div>Aiming at the bottleneck problems of limited visible light response range and high carrier recombination rate of Bi₂O₂CO₃ (BOC) photocatalyst, Ca²⁺/Ce³⁺ co-doped Bi₂O₂CO₃ composite photocatalyst was constructed by hydrothermal method. The analysis of different characterization techniques shows that double doping can lead to lattice distortion and induce oxygen defects. Under visible light irradiation, the degradation efficiency of ciprofloxacin (CIP) and levofloxacin (LVX) by BOC-Ca-Ce4 reached 92.56 % and 90.39 %, respectively, and maintained a certain degradation efficiency in complex water bodies such as tap water and lake water. ESR and capture experiments confirmed that •O₂⁻ and h⁺ were the dominant active species. Mechanism analysis showed that the valence cycle of Ce³⁺/Ce⁴⁺ and the local electric field of Ca²⁺ synergistically promoted the spatial separation of carriers. Through intermediate product analysis, CIP was finally mineralized to CO₂/H₂O, and the ECOSAR toxicity assessment showed that the ecological toxicity was reduced. This study provides a new design strategy for energy band engineering of two-component doped photocatalysts.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121668"},"PeriodicalIF":7.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics alter microbial structure and assembly processes in different soil types: Driving effects of environmental factors","authors":"Qingjie Li ,&nbsp;Xiaoxing Wang ,&nbsp;Jiaqi Zhang ,&nbsp;Xueqi Guo ,&nbsp;Yanli Li ,&nbsp;Okbagaber Andom ,&nbsp;Zhaojun Li","doi":"10.1016/j.envres.2025.121672","DOIUrl":"10.1016/j.envres.2025.121672","url":null,"abstract":"<div><div>Microplastics (MPs) are emerging pollutants with potential impacts on soil ecosystems. However, it is unclear how MPs-induced changes in the soil environment drive microbial structure and assembly in different soils. Here we investigated the responses of microbial structure, enzyme activities and soil properties to biodegradable polylactic acid (PLA) and conventional polythene (PE) with different doses in different soil types. Results showed that PLA generally decreased soil NH₄⁺-N and NO₃⁻-N levels but increased dissolved organic carbon (DOC) and pH, whereas PE exhibited contrasting effects depending on soil type. MPs significantly stimulated soil urease, sucrase, catalase and phosphatase activities, with dose-dependent responses observed under PLA treatments in fluvo-aquic soil. Additionally, MPs altered microbial composition and colonized specific bacterial taxa in different soils. In microbial assemblies dominated by stochastic processes, MPs, especially PE promoted the deterministic processes. Co-occurrence patterns showed lower microbial complexity under PLA treatments compared to PE. Notably, we revealed soil-type-specific response patterns: DOC emerged as the primary driver in red soil ecosystems, while pH exerted dominant control in fluvo-aquic soil systems. Furthermore, perturbation of microbial communities by MPs affected functions related to metabolism. These findings highlight that MPs-induced shifts in microbial communities and assembly processes are soil-type-specific and mediated by soil characteristics changes, providing critical insights for assessing the ecological risks of MPs in diverse agricultural soils.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121672"},"PeriodicalIF":7.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergy of multi-enzyme pretreatment and Paraclostridium benzoelyticum bioaugmentation: A dual strategy for enhancing methane production in dry anaerobic digestion of kitchen waste","authors":"Xueru Sun ,&nbsp;Panpan Hu ,&nbsp;Mengyao Xiao ,&nbsp;Siying Zhang ,&nbsp;Jiping Shi ,&nbsp;Dongqing Cai ,&nbsp;Dongfang Wang ,&nbsp;Lin Xu ,&nbsp;Li Liu ,&nbsp;Yanan Liu","doi":"10.1016/j.envres.2025.121671","DOIUrl":"10.1016/j.envres.2025.121671","url":null,"abstract":"<div><div>Dry anaerobic digestion (DAD) of kitchen waste (KW) has low methane production due to the poor mass transfer and the low abundance of functional microorganisms. This study employed multi-enzyme pretreatment (PRE), bioaugmentation with <em>Paraclostridium benzoelyticum</em> (BIO), and their combination (COM) to enhance methane production. Interestingly, the COM group had the highest methane production, which was increased by 18.51 %, 9.91 % and 12.39 % compared with the control, PRE and BIO groups, respectively, which indicated that there was a synergy between multi-enzyme pretreatment and bioaugmentation. Further analysis of microbial community and metagenome was conducted to reveal the synergistic mechanism. The results showed that in COM group, the enrichment of the Rikenellaceae, Methanobacteriaceae and Methanosaetaceae was the directly reason for enhancing methane production. Additionally, key metabolic functions including biosynthesis of cofactors, methane metabolism and oxidative phosphorylation also played a pivotal role in boosting methane production. Furthermore, the enhancement of the hydrogenotrophic methanogenesis pathway has been demonstrated to be a critical factor in the synergistic effects. It provided a reliable theoretical basis for the practical application of the multi-enzyme pretreatment combined with <em>Paraclostridium benzoelyticum</em> bioaugmentation for DAD.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"278 ","pages":"Article 121671"},"PeriodicalIF":7.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}