Environmental Research最新文献

{"title":"Functionalized Biochar Catalysts: Advancing Green Chemistry in Synthesis of O- and N-Heterocycles.","authors":"Nida Khan, Ashna Gupta, Shakir Ahamad, Mohd Kamil Hussain, Mohd Umar Khan, Zeba N Siddiqui","doi":"10.1016/j.envres.2025.122136","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122136","url":null,"abstract":"<p><p>The global shift toward sustainable chemistry has catalyzed the development of biochar as a versatile and eco-friendly catalyst in organic synthesis. Biochar, derived from biomass pyrolysis, offers tunable surface functionalities, high stability, and cost-effectiveness, establishing it as a viable alternative to conventional catalyst support. This review offers the first comprehensive overview of recent advancements in functionalized biochar-based catalysts for the synthesis of O and N-heterocycles. Particular focus is given to their applications in forming pharmaceutically and industrially important heterocycles, including chromenes, pyranopyrazoles, pyrazolopyridines, tetrazoles, oxindole dihydropyridines, benzylpyrazolyl coumarins, quinazolinones, spirochromenes, furoquinolines, and cyclic carbonates. This review explores the synthesis, functionalization, and catalytic performance of biochar, predominantly prepared via pyrolysis of diverse, renewable feedstocks such as chicken manure, neem bark, and wheat straw. These catalysts exhibit tailored properties, including large surface area, tunable porosity, and functional enhancements (e.g., by increasing reaction rates, selectivity, etc.) through the incorporation of magnetic, acidic, or metallic components. The review provides comparative insights into catalyst reusability, reaction efficiency, and mechanistic pathways, highlighting biochar's ability to replace hazardous reagents in heterocyclic synthesis. Additionally, examples are categorized by heterocycle type, offering detailed analyses of catalyst design, functionality, and advantages. The findings underscore the transformative potential of biochar-based catalysts in advancing green chemistry, while also pointing to opportunities for further innovation in this emerging field.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122136"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residential Greenness and Adolescent Mental Health Trajectories: A Longitudinal Pre-registered Study.","authors":"Sara Rudbæk Larsen, Divyangana Rakesh, Sarah Whittle, Nicholas B Allen, Peter G Enticott, Lærke Mygind","doi":"10.1016/j.envres.2025.122150","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122150","url":null,"abstract":"<p><p>Exposure to natural environments, such as residential green spaces, have been proposed as a promising approach to health promotion and prevention of mental health issues among adolescents. This study investigated the association between residential greenness and trajectories of adolescent mental health. PARTICIPANTS: (n=245) were part of the Orygen Adolescent Development Study and reported on anxiety and depression symptoms, using the Beck Anxiety Inventory and Center for Epidemiologic Studies Depression scale, at ages 12, 15, 17, and 19. Parent-reports of externalizing behavior was assessed with the delinquent behavior and aggressive behavior scales from the Child Behavior Checklist were collected at the same time points. Residential greenness was measured using the Normalized Difference Vegetation Index (NDVI) at baseline within a 1600m road network radius. Robust linear mixed models were used to examine associations between NDVI and within-subject changes in mental well-being, allowing for the inclusion of participants with only one data point while accounting for missing data. An exploratory analysis examined the interaction with sex in the association between NDVI and mental health. None of the models investigating residential greenness and mental health trajectories indicated consistent associations. The application of alternative buffer zones or distance to nearest park did not alter the conclusion. Sensitivity analysis investigating the moderating effect of childhood maltreatment did not reveal notable effects. Exploratory analyses, however, found a link between higher levels of childhood residential greenness and decreases in externalizing symptoms over time in males (β: -0.31 [95% CI -0.56, -0.06]), though no such observation was observed in females. Our study adds to the existing literature by highlighting the complex relationship between nature exposure and adolescent mental health. It emphasizes a need for future investigations to adopt a life-course approach and further explore potential sex differences and intersections of adolescent social identities with the potential benefits of nature.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122150"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of web-based behavioral intervention on fine particulate matter, pulmonary function, and airway inflammation in children: The COCOA randomized controlled trial.","authors":"Dirga Kumar Lamichhane, Bo-Mee Kim, Jungyun Bae, Hea Young Oh, Dong In Suh, Youn Ho Shin, Kyung Won Kim, Kangmo Ahn, Hyo-Bin Kim, Song-I Yang, So-Yeon Lee, Soo-Jong Hong, Hwan-Cheol Kim","doi":"10.1016/j.envres.2025.122200","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122200","url":null,"abstract":"<p><strong>Background: </strong>Although web-based intervention programs are effective in changing health behavior, evidence of their effectiveness in relation to air pollution and respiratory health in children is lacking. We assessed the effects of web-based behavioral intervention on exposure to fine particulate matter (PM ≤2.5μm in diameter [PM_2.5]), lung function, and airway inflammation in children.</p><p><strong>Methods: </strong>We randomized 80 mother-child pairs into intervention or control groups (two arms, 1:1 allocation). Personal and indoor PM_2.5 concentrations over a sampling period of 24 hour up to four occasions during the study period were measured in participants' homes. We used linear mixed models to assess the intervention effects on PM_2.5 concentration, lung function parameters including forced vital capacity (FVC), forced-expiratory volume in 1 second (FEV1), FEV1/FVC, and forced-expiratory flow at 25-75% (FEF_25%-75%), and the airway inflammation marker, fractional exhaled nitric oxide (FeNO), as well as the association of PM_2.5 with lung function and airway inflammation. Quantile regression was also used to examine the effects of PM_2.5 exposure at different quantiles of the outcome distribution.</p><p><strong>Results: </strong>In comparison with the control group, the intervention group showed reduction in indoor and personal PM_2.5 concentrations by 20.5 % (95% confidence interval [CI]: -30.7, -8.7) and 12.9% (95% CI: -20.1, -5.1), respectively. Lung function parameters such as FVC, FEV1, and FEF_25%-75% were higher in the intervention group, with greater benefits observed for children at the lower end of these parameters. Higher levels of outdoor and personal PM_2.5 (≥90^th percentile) were negatively associated with these lung function parameters at the lower quantiles, whereas the higher level of outdoor PM_2.5 concentration was positively associated with FeNO at the lower quantile.</p><p><strong>Conclusions: </strong>The behavioral intervention reduced PM_2.5 concentration in the homes, which was linked to markers of lung function and airway inflammation in children, particularly at the lower quantiles.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122200"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using the Pollution Load Index to Evaluate Rooftop Harvested Rainwater Metal(loid) Contamination in Environmental Justice Communities.","authors":"God'sgift N Chukwuonye, Kunal Palawat, Robert A Root, Luz Imelda Cortez, Theresa Foley, Victoria Carella, Charles Beck, Mónica D Ramírez-Andreotta","doi":"10.1016/j.envres.2025.122187","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122187","url":null,"abstract":"<p><p>Water scarcity poses a significant public health crisis exacerbated by climate change-induced disruptions to freshwater sources. Rainwater harvesting offers a sustainable solution by harnessing rooftop runoff for domestic use. This study analyzed 577 rooftop-harvested rainwater (RHRW) samples from four Arizona, USA environmental justice communities and 162 control samples from five National Atmospheric Deposition Program wet-only deposition collection sites across Arizona. The samples were tested for metal(loid)s, and the pollution load index (PLI) and Nemerow Integrated Pollution Index (NIPI) were used to assess contamination. The PLI was calculated for 11 known contaminants (As, Pb, Cd, Mn, Al, Cr, Cu, Zn, Ni, Ba, and Be), with the highest contamination factor observed for Ni (1340). PLI levels ranged from 0.118-65.8, with the active mining community Globe-Miami showing the highest range (0.244-65.8). The PLI was significantly greater during the monsoon season than during the winter season for all the communities (p < 0.05). Compared with urban communities (0.118-13.1), active mining communities (0.169-65.8) had higher PLI values. pH was positively correlated with PLI in Tucson (β = ln 0.27). In non-urban/rural mining communities, locations closer to potential contamination sources had higher PLI values (β = ln -0.33 to -0.38). However, in Tucson, the proximity relationship was less defined because of multiple potential contamination sources in urban areas. This study highlights the importance of using indices like PLI and NIPI to assess water quality; PLI reflects cumulative contamination burden, while NIPI contextualizes this burden within potential water uses. The strong positive correlation observed between PLI and NIPI across all use categories supports the validity of both indices and affirms utility. Together, they provide a nuanced understanding of pollution dynamics in RHRW and strengthen the case for public health interventions and ensuring the safety and sustainability of RHRW.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122187"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-pathway reactive species evolution over novel N-doped Bi₉O_13.5Cl₂/g-C₃N₄ scaffolding heterojunction : Investigation of dark and light-induced micropollutant decomposition.","authors":"Zhilin Yang, Yinzhu Zhou, Hao Xue, Yushi Xie, Haidi Zhang, Boaiqi Zhang, Binglin Guo, Na Liu, Lingsong Zhang, Fansheng Meng, Qi Yang, Yeyao Wang","doi":"10.1016/j.envres.2025.122164","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122164","url":null,"abstract":"<p><p>The mechanism underlying reactive species (RSs) evolution in photo-based advanced oxidation processes (AOPs) under continuous conditions has not been well characterized and investigated in the existing scientific literatures. A scaffolding heterojunction comprising N-doped graphitic carbon nitride with novel Bi₉O_13.5Cl₂ photocatalyst (NBCN) was successfully fabricated, achieving 96.7% for TC degradation compared to 63.4% for non-doped BCN. Comprehensive characterization analyses demonstrated exceptional charge-carrier separation dynamic through composite heterojunction. Under illuminated conditions, we identified two main RSs formation routes: an O₂-mediated pathway and a h⁺-mediated pathway. Comparative kinetic analysis revealed the critical role of O₂ activation, showing a remarkable 6.2-fold enhancement in kinetic rate constant for organic pollutant degradation under O₂-ambient condition (k = 3.1 × 10^-2 min^-1) compared to the anaerobic environment (k = 5 × 10^-3 min^-1). Primary RSs were identified and ranked in order of significance as ¹O₂ >^•O₂^- >h⁺ >^•OH. Theoretical calculations further revealed that the promoted photocatalytic performance was achieved through favorable adsorption energy, promoted electron redistribution by N-doping and well-defined formation pathways of O₂-mediated RSs. Under dark conditions, significant amounts of dark-generated h⁺ were detected in NBCN, where its hydrophilic nature facilitated the formation of transient ^•OH. The practical applicability of the system was validated through real water matrix tests, highlighting the robust performance of the O₂ activation and ¹O₂ production. By elucidating the mechanism of continuous contaminant degradation under entire round-the-clock scenarios, this study advances the development of sustainable environmental remediation technologies in low temperature environment across various water matrices.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122164"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroactive biofilm enhances synergistic degradation of sulfamethoxazole and roxarsone in actual livestock wastewater: extracellular electron transfer drives metabolic network remodeling.","authors":"Pin Peng, Xinyu Yan, Lixiang Chen, Xiang Li, Huiying Yang, Yijing Miao, Feng Zhao","doi":"10.1016/j.envres.2025.122173","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122173","url":null,"abstract":"<p><p>Livestock wastewater is characterized by high concentrations of organic matter and diverse antibiotics. A major challenge in its anaerobic treatment is the effective degradation and thorough removal of coexisting antibiotic contaminants. In this study, an external voltage was applied to establish an electroactive biofilm, thereby enhancing the co-metabolic degradation of sulfamethoxazole and roxarsone. Application of +0.6 V (Ag/AgCl) increased the 72 h TOC removal efficiency to 52.1%. At the same time, sulfamethoxazole and roxarsone removal efficiencies reached 90.8% and 100%, respectively. The results from liquid chromatography-mass spectrometry (HPLC-MS/MS) and density functional theory (DFT) calculations revealed sulfamethoxazole degradation pathways: N-O bond cleavage and hydroxylation. Roxarsone degradation primarily involved nitro group reduction and C-As bond cleavage. The pharmacophore of sulfamethoxazole was effectively removed, and inorganic arsenic from roxarsone degradation was efficiently immobilized. Toxicity analysis confirmed that the electroactive biofilm notably reduced toxic intermediate accumulation. Elevated concentrations of NADH and ATP in the electroactive biofilm indicated enhanced microbial substrate metabolism and generation of more electron donors. The higher absolute abundance of the sulfamethoxazole degradation gene (SadABC) in the electroactive biofilm indicated that sulfamethoxazole degrading enzyme (sadABC) gained more electrons. Microbiome analysis revealed the upregulation of genes linked to extracellular electron transfer (EET), the tricarboxylic acid (TCA) cycle, nitro-reduction, and sulfate reduction pathway, confirming the electroactive biofilm enhances substrate metabolism and co-metabolic antibiotic degradation. Electroactive biofilm offers a viable strategy for antibiotic removal in livestock wastewater.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122173"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Factors Mediate Mercury Exposure in Fish with Diverse Feeding Habits in the Beibu Gulf.","authors":"Feng Zeng, Le Chao, Yang Yan, Zheng Guo, Liangliang Huang, Yunrong Yan, Jiachen Sun, Bin Kang","doi":"10.1016/j.envres.2025.122190","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122190","url":null,"abstract":"<p><p>Tropical and subtropical oceans, including the Beibu Gulf, a key fishing area in China, receive a substantial share of global mercury (Hg) deposition, yet the exposure levels and influencing factors in marine organisms remain underexplored. This study analyzed total and methylated Hg (THg and MeHg) concentrations in 650 fish from 10 species across 24 sites in the eastern Beibu Gulf in April and August 2022, along with concurrent surface water parameter measurements. The significant variations in fish Hg content across species were primarily driven by feeding habits, with benthic feeders exhibiting notably higher Hg levels than nekton and zooplankton feeders. Among 13 seawater parameters, temperature and pH were found to significantly influence Hg concentrations across species, with these effects varying by feeding guild. As sea temperature rose, MeHg levels increased in nekton-feeding fish but decreased in zooplankton-feeding fish, whereas in benthos-feeding species, MeHg levels showed a significant negative correlation with pH rather than temperature. Hg levels in Upeneus sulphureus and Johnius belangerii pose moderate and low risks for human consumption, respectively, with long-term consumption potentially reducing neonatal IQ by 0.45 points and increasing the risk of fatal heart attacks by 2.68 cases per 100,000 person-years in adults.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122190"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking Early-Life PFAS Exposure in Children at Ages 4, 8, and 14 years: A Longitudinal Study from the INMA Spanish Birth Cohort.","authors":"Anne San Román, Eunate Abilleira, Belen Gonzalez-Gaya, Juan Francisco Ayala-Cabrera, Ziortza Barroeta, Nestor Etxebarria, Amaia Irizar","doi":"10.1016/j.envres.2025.122198","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122198","url":null,"abstract":"<p><p>In recent times, the widespread use of PFAS, combined with their persistence and mobility, has led to global environmental contamination and human bioaccumulation. Mapping PFAS exposure in children at critical developmental stages (4, 8, and 14 years) is essential to assess early exposure and its relationship with living place, biological sex and potential health outcomes. In this work, plasma samples from two regions of Gipuzkoa have been analyzed from 207 children at early childhood, 241 children, and 167 adolescents in the INMA Gipuzkoa Spanish birth cohort (2011-2022) for 42 targeted PFAS. The results confirmed 18 different PFAS, including long and short-chain congeners, precursors, and emerging PFAS. The most detected PFAS were PFOS, PFOA, PFNA, PFDA, and PFHxS, with detection frequencies of 70-97%. At 4 years, children showed the highest detection frequencies, while short-chain PFAS detection (PFBA, FPrPA, PFBS, PFPeA) increased when they reached puberty indicating a change on the environmental exposure patterns. The samples coming from the Urola region showed higher concentrations, exceeding the EFSA limit for chronic exposure to sum of 4 PFAS (PFNA, PFOA, PFOS, PFHxS) in 26-35% of 14-year-old males and females. PFOA concentrations, in particular, increased between the 8- and 14-year follow-up in this region indicating an exposure between follow-ups. No significant differences were found between sexes for the total PFAS burden but for individual PFAS with overall higher levels in males at 14 years. Hazard quotient assessments employing points of departure taken from literature indicated no risk or moderate risk for hepatic and developmental effects in the general studied population. Finally, multivariate linear regression models showed that maternal PFAS levels and breastfeeding duration were positively associated with children's PFAS concentrations in early development, while environmental or regional factors became more influential with age. Parental socioeconomic status did not emerge as a significant predictor in the models.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122198"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial heterogeneous associations and spillover effects of ozone pollution in China","authors":"Xin Zhang ,&nbsp;Wei Wu ,&nbsp;Hong-Bin Liu","doi":"10.1016/j.envres.2025.122201","DOIUrl":"10.1016/j.envres.2025.122201","url":null,"abstract":"<div><div>Surface ozone pollution has become a major environmental concern in China. Understanding its spatial associations at both intra-stratum and inter-strata levels is essential for effective pollution control. However, research on spatial stratified heterogeneity and spillover effects of ozone pollution remains limited. This study applies a hybrid model integrating random forest, SHapley Additive exPlanations, and geographically weighted regression to investigate the spatial heterogeneity and spillover effects of ozone pollution. Principal component analysis and geographically and temporally weighted regression were further used to assess local environmental drivers of ozone spillover. The results indicate a fluctuating upward trend in ozone concentrations with enhanced spatial clustering. Intra-stratum associations in South, Central, and East China and inter-strata associations in Northeast and North China exhibited strengthening trends. Ozone spillover levels were highest in North China, with a slight decline, followed by Northwest and Northeast China, where spillover decreased more markedly. In contrast, Southwest, East, South, and Central China—regions with previously low spillover levels—showed a fluctuating increase. Industrial structure was the primary driver of ozone spillover across all regions and years; regions historically dominated by the secondary sector experienced spillover reduction following structural adjustments. Technology expenditure mitigated ozone spillover in North China but intensified it in Southwest China. The effects of relative humidity, ventilation coefficient, and mean sea level pressure on ozone spillover emerged in Central and Eastern China.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"283 ","pages":"Article 122201"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EpicPCR-Based Identification of Diversity and Community Structure of Environmental alkBs-Carrying Bacteria.","authors":"Tianhua Lv, Yunke Cao, Shuai Chen, Wenzhuo Tian, Yuan Yun, Guoqiang Li, Ting Ma","doi":"10.1016/j.envres.2025.122167","DOIUrl":"https://doi.org/10.1016/j.envres.2025.122167","url":null,"abstract":"<p><p>Oil spills during transport have resulted in severe hydrocarbon contamination in soil and water. As endogenous microorganisms, hydrocarbon oxidizing bacteria (HOB) play a vital role in degrading hydrocarbons in oil-contaminated environments. However, accurately identifying and tracking HOB distribution remain difficult due to the low conservative and high diversity of their key alkane monooxygenase genes (alkBs). Here, we proposed a workflow with multiple-primers-based epicPCR processes to identify HOBs in the environment. Epic-alkB database, which contained 14 times more genera and 6 times more phyla than the known database (AlkBase, 23 genera and 4 phyla) was built successfully with 5 pairs of major primers, and some of the genera coexist in both databases or specific to the epic-alkB database were functionally validated by newly isolated HOBs. The addition of multiple primer pairs enabled us to obtain accurate HOBs with higher coverage and diversity than AlkBase. Besides, the alkB genes varied among microorganisms. unclassified_Ignavibacteriales, Pseudomonas, and Thalassolituus were mainly amplified by alkB_4/5/6 respectively, with alkB_6 being the most prevalent and abundant. In conclusion, an epicPCR-based workflow was provided to facilitate the accurate identification of HOBs and the exploration of the distribution of alkB genes in the biosphere, which greatly expands the bacterial species for aerobic hydrocarbon degradation and offers a universal approach for the identification and quantification of isozyme genes in the environment.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"122167"},"PeriodicalIF":7.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}