Environmental Research最新文献

{"title":"Microbial mechanism and CO2 emission from coastal saline soil: The role of corn stover and nutrient additions","authors":"Qiao Xu ,&nbsp;Ling Zhang ,&nbsp;Yingdong Zhao ,&nbsp;Caixian Tang ,&nbsp;Yinjuan Li ,&nbsp;Meiling Xu ,&nbsp;Xiaozhi Wang","doi":"10.1016/j.envres.2025.121423","DOIUrl":"10.1016/j.envres.2025.121423","url":null,"abstract":"<div><div>Coastal saline soils are increasingly reclaimed for agricultural purposes through organic amendments and nutrient supplementation, yet their impacts on soil organic carbon (SOC) and inorganic carbon (SIC) dynamics remain poorly understood. A microcosmic incubation experiment with ¹³C-labeled corn stover and/or nitrogen (N) and phosphorus (P) additions was established to examine the contributions of SOC and SIC to soil total CO₂ efflux and the associated microbial mechanisms. Corn stover addition increased soil NO₃⁻-N, inorganic N, available P and potassium (K), and dissolved organic C (DOC) by 50 %, 41 %, 22 %, 8 % and 52 %, respectively, while nutrients alone increased them by 73 %, 170 %, 128 %, 10 % and 16 %. Total CO₂ efflux rose by 35 % with stover and by 24 % with nutrients alone, but their combined application synergistically enhanced emissions by 87 %. Stover addition initially increased CO₂ effluxes from both SIC and SOC when nutrients were not amended but reduced these fluxes when nutrients were supplied during the first week. Microbial diversity declined under both amendments, with a shift toward copiotrophic taxa (e.g. <em>Salinimicrobium</em> and <em>Microbulbifer</em> as keystone bacterial genera) and reduced resilience to disturbance. Notably, SIC contributed 25 %–40 % of total CO₂ efflux, highlighting its sensitivity to agricultural management. These findings underscore that SIC dissolution-driven by microbial or chemical processes—plays a substantial role in coastal soil carbon emissions. Future research must clarify the mechanisms of carbonate dissolution to refine global C cycling models in the context of agricultural expansion into coastal mudflats.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121423"},"PeriodicalIF":7.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced ammonium oxidation and iron cycle of Feammox under micro-oxygen condition","authors":"Tuo Wang ,&nbsp;Mou Zhang ,&nbsp;Nana Jiang ,&nbsp;Xinlei Jiang ,&nbsp;Nan Li ,&nbsp;Fernanda Leite Lobo ,&nbsp;Mei Chen ,&nbsp;Xin Wang","doi":"10.1016/j.envres.2025.121443","DOIUrl":"10.1016/j.envres.2025.121443","url":null,"abstract":"<div><div>Autotrophic anaerobic ammonium oxidation coupled to Fe(III) reduction (Feammox) is a promising technology for treating low C/N wastewater. However, Feammox still faces bottlenecks of slow ammonium oxidation rate and the continuous supply of Fe(III) source. This study adopts micro-oxygen strategy to overcome these obstacles. Micro-oxygen increased the ammonium oxidation rate up to 5.7 times higher than under anaerobic condition, and drove the iron cycle in the form of vivianite [Fe(II)] and leucophosphite [Fe(III)]. Furthermore, it was confirmed that the ammonium oxidation in Feammox relies on ammonia monooxygenase (AMO), as evidenced by 10 times increase in the relative <em>amo</em> expression and 1.2 times increase in AMO activity under micro-oxygen compared to anaerobic condition. Additionally, this approach enhanced the growth and co-metabolism of functional bacteria. Long-term experiments demonstrated the sustainability of the Feammox system with iron cycle under micro-oxygen condition. These findings provide valuable insights into the practical application of Feammox process.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121443"},"PeriodicalIF":7.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and physiological responses of black rockfish (Sebastes schlegelii) to short- and medium-term ocean acidification","authors":"Yunhong Gao ,&nbsp;Jianchao Li ,&nbsp;Wenwen Wang ,&nbsp;Yongjun Tian","doi":"10.1016/j.envres.2025.121431","DOIUrl":"10.1016/j.envres.2025.121431","url":null,"abstract":"<div><div>Ocean acidification (OA) is one of the greatest threats to marine species, with widespread impacts on their physiological functions. However, the adaptive capacities of many marine species to OA and the underlying mechanisms remain unclear. In this study, we investigated the effects of short-term (4 days) and medium-term (30 days) CO₂ exposure (pH 8.0, 7.6, and 7.3) on black rockfish (<em>Sebastes schlegelii</em>), focusing on histopathological changes in gill tissues, ion transport biomarkers, oxidative stress indicators, and transcriptomic responses. The results showed that both short-term and medium-term OA induced significant morphological changes in gill tissues, including epithelial lifting, hyperplasia, hypertrophy, and lamellar clubbing, which are likely adaptive mechanisms for maintaining homeostasis. Both Na⁺/K⁺-ATPase and carbonic anhydrase (CA) activities increased significantly in both short- and medium-term exposure, while Ca²⁺-ATPase activity was elevated only in the short-term, suggesting differential enzyme regulation over time to sustain ionic balance. Additionally, oxidative stress indicators (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), reduced glutathione (GSH) and glutathione peroxidase (GPx)) were significantly elevated after both exposure durations, indicating that the antioxidant defense system was activated. Moreover, the integrated biomarker response (IBR) index further indicated that the stress response was more pronounced during short-term exposure. Transcriptomic analysis reveals significant alterations in pathways related to calcium signaling, cytoskeletal structure, energy metabolism, and oxidative stress following short-term exposure. In contrast, medium-term exposure leads to significant enrichment of pathways associated with cell-environment interactions, highlighting the molecular adaptations of <em>S. schlegelii</em> to OA-induced stress. These findings provide valuable insights into the mechanisms of OA tolerance in <em>S</em>. <em>schlegelii</em> and contribute to understanding the adaptability of marine species in future ocean environments.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121431"},"PeriodicalIF":7.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autotrophic Uranium (VI) bioreduction in groundwater by siderite: Comparison with sulfur and zero-valent iron","authors":"Yidan Zhang ,&nbsp;Meng Ren ,&nbsp;Chuanjin Xia ,&nbsp;Wenzhong Wang ,&nbsp;Yuling Zhu","doi":"10.1016/j.envres.2025.121417","DOIUrl":"10.1016/j.envres.2025.121417","url":null,"abstract":"<div><div>Uranium (U), recognized as a significant health risk in groundwater, has become a key focus in environmental remediation efforts. While numerous electron donors have been investigated for the removal of U(VI) through microbial processes, the potential of abundant and economical Fe(II)-containing minerals remains unexplored.</div><div>Here, a new inorganic electron donor, siderite (FeCO₃) was proposed. Although siderite demonstrates a lesser electron-donating capacity than Fe(0) and S(0), the Siderite-B bioreactor successfully enriched microbes belonging to the <em>Azotobacter</em> genus, which are known for their nitrogen-fixing ability. Within this system, <em>Azotobacter</em> facilitated the oxidation of Fe(II) coupled with the reduction of U(VI). Initially, Fe(II) donated electrons to the NAD⁺/NADH couple. Subsequently, NADH transferred these electrons to the Rnf/Fix complex, which in turn donated them to ferredoxin, catalyzing the reduction of U(VI) to U(IV). The Siderite-B autotrophic bioreactor achieved a U(VI) removal efficiency of 93.40 ± 0.47 % over 144 h, which was slightly lower than the S(0)-B bioreactor (97.12 ± 0.50 %) and the Fe(0)-B bioreactor (95.58 ± 0.95 %). In contrast, S(0)-B and Fe(0)-B bioreactors were enriched with microbes belonging to the <em>Thiobacillus</em> genus, which reduced U(VI) mainly through Fe-S oxidoreductase and Cytochrome C mediated electron transfer. Over a 90-day continuous-flow experiment, the Siderite-B bioreactor exhibited high U(VI) removal efficiencies of 96.83 ± 1.12 %, 95.92 ± 1.84 %, and 85.28 ± 1.41 % at influent U(VI) concentrations of 10, 20, and 30 mg/L, respectively. The findings highlight the potential of siderite as an effective and autotrophic electron donor for U(VI) reduction, offering a cost-effective and environmentally friendly alternative for groundwater uranium remediation.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121417"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contaminant bioaccumulation and biochemical responses of the bivalve Scrobicularia plana and the polychaete Hediste diversicolor to ecosystem restoration measures using Zostera noltei","authors":"V.H. Oliveira ,&nbsp;B. Marques ,&nbsp;A. Carvalhais ,&nbsp;D. Crespo ,&nbsp;M. Dolbeth ,&nbsp;A.I. Sousa ,&nbsp;A.I. Lillebø ,&nbsp;M. Pacheco ,&nbsp;M.E. Pereira ,&nbsp;S. Díez ,&nbsp;J.P. Coelho ,&nbsp;C.L. Mieiro","doi":"10.1016/j.envres.2025.121429","DOIUrl":"10.1016/j.envres.2025.121429","url":null,"abstract":"<div><div>A Nature-based Solution (NbS) using <em>Zostera noltei</em> transplants was implemented to restore an area historically contaminated with metals and enhance local environmental conditions. However, the benefits of this restoration approach on the health of resident benthic communities remained unclear, considering the time span of one year of implementation. This study evaluated the short-term effects of transplantation by evaluating bioaccumulation and biochemical responses in <em>Scrobicularia plana</em> and <em>Hediste diversicolor</em>. Overall, the transplanted vegetation played a crucial role in controlling contaminant accumulation, with both species exhibiting lower contaminant levels in vegetated sites compared to bare-bottom sites. The study also revealed species-specific responses to oxidative stress, antioxidant defences and energy budgets, with <em>H. diversicolor</em> being more vulnerable to the absence of vegetation than <em>S. plana</em>. These findings highlight the critical role of vegetation in improving environmental quality and promoting the health of benthic communities, validating the positive effect of the applied restoration measures.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121429"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal exposure to ozone and bone mineral density in early childhood: Susceptible window identification","authors":"Linxi Tang ,&nbsp;Yibing Zhu ,&nbsp;Yudiyang Ma ,&nbsp;Chong Miao ,&nbsp;Bin Sun ,&nbsp;Feipeng Cui ,&nbsp;Jingmin Guo ,&nbsp;Yaohua Tian","doi":"10.1016/j.envres.2025.121419","DOIUrl":"10.1016/j.envres.2025.121419","url":null,"abstract":"<div><h3>Background</h3><div>The effect of prenatal ozone exposure on early childhood bone mineral density (BMD) remains to be explored.</div></div><div><h3>Methods</h3><div>This study was based on the pediatric subcohort of the Fujian Birth Cohort Study. The mean ozone exposure concentration was calculated for the entire pregnancy, as well as for each trimester (first, second, and third) and for every gestational week. BMD was assessed using quantitative ultrasonography (QUS). Low BMD was defined as a z-score of less than −1. Linear and logistic regression models were used to assess the effect of maternal ozone exposure. Distributed lag models (DLMs) were applied to identify the sensitive windows of ozone exposure.</div></div><div><h3>Results</h3><div>1345 mother-offspring pairs were included in this study. We found that for every interquartile range (IQR) increase in ozone levels of different exposure durations (entire pregnancy, first, second, and third trimester), BMD z-scores decreased by 0.12 (95 % CI: 0.18, −0.07), 0.07 (95 % CI: 0.11, −0.02), 0.08 (95 % CI: 0.14, −0.02), and 0.07 (95 % CI: 0.13, −0.01), respectively. For low BMD, an IQR increase in ozone levels was associated with 1.47 times higher odds of low BMD (95 % CI: 1.09, 1.98) in the second trimester. The most pronounced negative association with BMD z-scores was observed between the 9th and 16th gestational weeks. For low BMD, the highest OR was observed between the 15th and 17th gestational weeks.</div></div><div><h3>Conclusions</h3><div>Prenatal exposure to ozone was inversely associated with BMD in early childhood, with early to mid-pregnancy identified as the susceptible window.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121419"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to \"Effects of vermicompost on soil microbiological properties in lettuce rhizosphere: An environmentally friendly approach for sustainable green future\" [Environ. Res. 243 (2024) 117737].","authors":"Muhammad Danish Toor, Ridvan Kizilkaya, Aneela Anwar, Lyubka Koleva, Gaber E Eldesoky","doi":"10.1016/j.envres.2025.121396","DOIUrl":"https://doi.org/10.1016/j.envres.2025.121396","url":null,"abstract":"","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"121396"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between objectively assessed physical activity and kidney function among female agricultural workers in hot environments in Spain.","authors":"Sarah Koch, Joren Buekers, Ana Espinosa, Juan Gómez-Salgado, Gabriel Pombo, Loes Werkman, Lourdes Arjona, Ali Al Rashed, Ben Caplin, Manolis Kogevinas, Francisco Brocal Fernandez, Amin Oomatia, Neil Pearce, Oriana Ramirez-Rubio, Carlos Ruíz-Frutos, Judith Garcia-Aymerich, Cristina O'Callaghan-Gordo","doi":"10.1016/j.envres.2025.121420","DOIUrl":"https://doi.org/10.1016/j.envres.2025.121420","url":null,"abstract":"<p><strong>Background: </strong>Physical activity in hot environments has been associated with impaired kidney function. We aimed to quantify the association between occupational physical activity and kidney function over one work shift in female agricultural workers exposed to environmental heat.</p><p><strong>Methods: </strong>We measured occupational physical activity in female berry pickers in Huelva, Spain, using movement intensity, based on vector magnitude units (VMU), and heart rate (HR). For both, we calculated the mean (VMU_mean;HR_mean), standard deviation (VMU_sd; HR_sd), and 3-minute 95^th percentile (VMU_p95;HR_p95). Environmental heat in greenhouses was estimated using wet bulb globe temperature (WBGT). Kidney function and injury were quantified using pre- and post-shift differences in serum creatinine (SCr_dif), Cystatin C (Cystatin C_dif) and neutrophil gelatinase associated lipocalin (NGAL_dif), respectively. Acute Kidney Injury (AKI) was defined as an increase in post-shift serum creatinine of ≥0.3mg/dl, or ≥1.5 times pre-shift levels. Heat strain was estimated using the ISO 7933:2004 index. We used linear and logistic regressions.</p><p><strong>Results: </strong>Ninety women aged 37.4 ± 5.7 years were exposed to a mean WBGT of 25.2 (±3.6)°C and light intensity physical activity (HR_mean of 94 (±9)bpm). Of those, 26% experienced heat strain, 68% were dehydrated, and 7% presented with AKI at the end of the shift. Higher VMU_sd was associated with higher Scr_dif (β:0.029 (95% CI: 0.00; 0.058) and meeting the threshold for AKI (OR:1.6 (95% CI:0.8; 3.2)), We observed higher NGAL_dif with higher VMU_mean (β:1.944 (95% CI: 0.139;3.748)) per 100 VMU_mean increase. The positive association between HR_mean and SCr_dif was stronger among women with heat strain (interaction p = 0.033).</p><p><strong>Conclusions and relevance: </strong>In female harvest workers, high means and variations in VMU over a single one shift worked under hot conditions were associated with kidney injury, as assessed by urinary biomarkers, though there is limited evidence for any change in kidney function. Precautiously, large changes in physical activity intensity over one work shift should be avoided to protect from renal injury.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"121420"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediation role of gut microbiota in the association between ambient fine particulate matter components and cardiovascular disease: Evidence from a China cohort","authors":"Shaokun Yang ,&nbsp;Rui Yu ,&nbsp;Fen Yang ,&nbsp;Jiawei Li ,&nbsp;Lele Wang ,&nbsp;Gonghua Wu ,&nbsp;Liling Chen ,&nbsp;Tingting Yang ,&nbsp;Zhuoma Duoji ,&nbsp;Yanjiao Wang ,&nbsp;Xufang Gao ,&nbsp;Yuanyuan Liu ,&nbsp;China Multi-Ethnic Cohort (CMEC) collaborative group","doi":"10.1016/j.envres.2025.121421","DOIUrl":"10.1016/j.envres.2025.121421","url":null,"abstract":"<div><h3>Background</h3><div>Pairwise associations among fine particulate matter (PM_2.5), gut microbiota, and cardiovascular disease (CVD) have been established. However, the mediating role of gut microbiota in the relationship between PM_2.5 and its components and CVD remains unclear.</div></div><div><h3>Methods</h3><div>We included 1459 participants from the China Multi-Ethnic Cohort between May 2018 and September 2019. CVD was identified using ICD-10 codes based on hospital surveillance system. PM_2.5 and its components were sourced from the ChinaHighAirPollutants dataset. Gut microbiota was obtained from 16S rRNA sequencing of stool samples, and five α-indexes along with 1088 gut compositions were used as mediators. Cox proportional hazards and multiple linear regression were used to explore the associations among PM_2.5 and its components, gut microbiota, and CVD. Causal mediation analysis was conducted to evaluate the potential mediating role of gut microbiota between PM_2.5 and its components and CVD.</div></div><div><h3>Results</h3><div>Among all the participants, 204 (14.0 %) had developed CVD during a 5501 person‐year follow‐up (median, 3.8 years). The ACE, Chao1, and Obs indexes positively mediated the associations of PM_2.5 and its components with both CVD and stroke, with mediation proportions ranging from 7.9 % to 8.9 % for CVD and 10.0 %–12.1 % for stroke. The ACE index had the highest mediation proportion (12.1 %) in the relationship between sulfate and stroke. The genus Pasteurella also demonstrated a mediating role, accounting for 2.6 %–3.2 % for CVD, and 2.5 %–3.6 % for stroke, exhibiting the highest mediation proportion (3.6 %) on the association between black carbon or nitrate and stroke.</div></div><div><h3>Conclusion</h3><div>Three α-indexes (ACE, Chao1, and Obs) and the Pasteurella positively mediated the association between PM_2.5 and its components and CVD risk. Enhancing the richness of gut microbiota could potentially reduce the risk of CVD induced by PM_2.5 and its components.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121421"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil pH and total phosphorus regulate bacterial community assembly in slope restoration areas of the Tibetan Plateau's metal mining areas","authors":"Huanyu Zhou ,&nbsp;Xiaotong Liu ,&nbsp;Xianlei Gao ,&nbsp;Yan Wang ,&nbsp;Lanlan Ye ,&nbsp;Junxi Wu ,&nbsp;Mingxue Xiang","doi":"10.1016/j.envres.2025.121432","DOIUrl":"10.1016/j.envres.2025.121432","url":null,"abstract":"<div><div>Microbial community development is a crucial aspect of soil restoration. The employment of frame beams in conjunction with external soil has demonstrated efficacy in the rehabilitation of degraded roadside ecosystems within mining regions. Nonetheless, the effects of frame beams on the composition and stability of soil bacterial communities remain inadequately comprehended. We conducted a one-time soil sampling on a three-year restored slope in a large–scale metal mining area on the Tibetan Plateau, providing a snapshot of the current conditions and evaluating the restoration progress. Frame beams with external soil covers were applied at three different altitudes: A1 (4800–5000 m), A2 (4500–4700 m), and A3 (4200–4400 m). Restoration significantly altered bacterial community composition compared with controls. <em>Proteobacteria</em> had a higher relative abundance in the restoration area (average: 31.16 %), whereas <em>Acidobacteriota</em> were more abundant in the control area (average: 24.68 %). In the restoration area, soil bacterial α–diversity increased as elevation decreased, with the Shannon index rising from 5.34 (A1) to 5.82 (A3), suggesting that bacterial communities at higher altitudes are more sensitive to environmental conditions. Species turnover was the primary driving factor of β–diversity, accounting for 96.26 % under A1, 94.71 % under A2, and 91.94 % under A3, respectively. The nearest taxon index of bacterial communities shifted from negative to positive along the elevation gradient (−0.25 to 1.14), indicating an increasing trend toward community clustering. Within the bacterial co-occurrence network, soil pH and total phosphorus contribute significantly to network strength, closeness, and betweenness. Concluding, soil pH and total phosphorus were identified as key factors shaping bacterial diversity and assembly mechanisms. Our research contributes to the development of effective soil restoration strategies for alpine mining regions, providing insights into microbial community assembly and stability mechanisms.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"275 ","pages":"Article 121432"},"PeriodicalIF":7.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}