Environment International最新文献

{"title":"A comprehensive assessment of non-indigenous species requires the combination of multi-marker eDNA metabarcoding with classical taxonomic identification","authors":"Stefano Varrella ,&nbsp;Silvia Livi ,&nbsp;Cinzia Corinaldesi ,&nbsp;Luca Castriota ,&nbsp;Teresa Maggio ,&nbsp;Pietro Vivona ,&nbsp;Massimo Pindo ,&nbsp;Sebastiano Fava ,&nbsp;Roberto Danovaro ,&nbsp;Antonio Dell’Anno","doi":"10.1016/j.envint.2025.109489","DOIUrl":"10.1016/j.envint.2025.109489","url":null,"abstract":"<div><div>In marine environment, non-indigenous species (NIS) can alter natural habitats and cause biodiversity loss with important consequences for ecosystems and socio-economic activities. With more than 1000 NIS introduced over the last century, the Mediterranean Sea is one of the most threatened regions worldwide, requiring an early identification of newly entered alien species for a proper environmental management. Here, we carried out environmental-DNA (eDNA) metabarcoding analyses, using multiple molecular markers (i.e., <em>18S rRNA</em>, <em>COI</em>, and <em>rbcL</em>) and different genetic databases (i.e., NCBI, PR2, SILVA, MIDORI2, MGZDB, and BOLD), on seawater and sediment samples collected on a seasonal basis in three Mediterranean ports located in the North Adriatic, Ionian and Tyrrhenian Sea to identify marine species, and particularly NIS. The use of the multi-marker eDNA metabarcoding allowed the identification of a higher number of species compared to the morphological analyses (1484 vs. 752 species), with a minor portion of species shared by both approaches. Overall, only 4 NIS were consistently identified by both morphological and molecular approaches, whereas 27 and 17 NIS were exclusively detected by using eDNA metabarcoding and classical taxonomic analyses, respectively. The eDNA metabarcoding allowed also identifying the genetic signatures of 5 NIS never reported in the Italian waters. We conclude that eDNA metabarcoding can represent a highly sensitive tool for the early identification of NIS, but a comprehensive census of the NIS requires the combination of molecular and morphological approaches.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109489"},"PeriodicalIF":10.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urban heat island impacts on mental health in middle-aged and older adults","authors":"Yujia Bao ,&nbsp;Yongxuan Li ,&nbsp;Jiawei Gu ,&nbsp;Chen Shen ,&nbsp;Yuzheng Zhang ,&nbsp;Xiaobei Deng ,&nbsp;Lefei Han ,&nbsp;Jinjun Ran","doi":"10.1016/j.envint.2025.109470","DOIUrl":"10.1016/j.envint.2025.109470","url":null,"abstract":"<div><h3>Background</h3><div>Urban heat island (UHI), attributed to rapid urbanization, might be a latent modifiable risk factor for human health, yet little is known about whether UHI puts a strain on public mental health. This study aimed to assess the effect of the summer UHI on mental health.</div></div><div><h3>Methods</h3><div>Leveraging 338,363 urban residents from the UK Biobank, this study estimated the associations of summer UHI effect with incidence risks of mental disorders, substance use disorder, depressive disorder, and anxiety disorder using both time-independent and time-dependent Cox regression models with full adjustment for possible confounders. Furthermore, the effect of UHI on related psychiatric symptoms and brain microstructure were explored through logistic regression models and multiple linear regression models, respectively.</div></div><div><h3>Results</h3><div>In this study, summer UHI was significantly associated with the elevated risks of psychiatric disorders. The hazard ratio with a 95% confidence interval (CI) from the time-dependent Cox model was 1.04 (95% CI, 1.03–1.05) for mental disorders, 1.12 (1.11–1.14) for substance use disorder, 1.08 (1.06–1.10) for depressive disorder, and 1.06 (1.04–1.08) for anxiety disorder per standard deviation of UHI intensity, respectively. Subgroup analyses showed that females and individuals with hypertension or coronary artery disease were more vulnerable to the UHI effect on mental health. The detrimental effects on psychiatric symptoms and white matter microstructure were also observed.</div></div><div><h3>Conclusion</h3><div>The study suggested UHI could be an environmental stressor and induce a heavier burden on mental health. The effective mitigation of urban heat stress could benefit both public health and sustainable development.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109470"},"PeriodicalIF":10.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of Proteus mirabilis: Unraveling global epidemiology and antimicrobial resistance dissemination − emerging challenges for public health and biosecurity","authors":"Shaqiu Zhang ,&nbsp;Qianlong Li ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Shun Chen ,&nbsp;Mafeng Liu ,&nbsp;Dekang Zhu ,&nbsp;Xinxin Zhao ,&nbsp;Ying Wu ,&nbsp;Qiao Yang ,&nbsp;Juan Huang ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Bin Tian ,&nbsp;Yu He ,&nbsp;Zhen Wu ,&nbsp;Anchun Cheng","doi":"10.1016/j.envint.2025.109487","DOIUrl":"10.1016/j.envint.2025.109487","url":null,"abstract":"<div><div>Given the escalating public health threat posed by<!--> <em>Proteus mirabilis</em> <!-->(<em>P. mirabilis</em>) and its rapidly evolving drug resistance, it is imperative to elucidate its global epidemiology and resistance mechanisms through a comprehensive genomic lens. As of August 2024, 3,403 high-quality<!--> <em>P. mirabilis</em> <!-->genomes were retrieved from public databases (total 3,752), spanning 58 countries/regions, with the United States showing the highest report rate (52.51 %). Human-derived isolates, particularly from urine (34.47 %), were the primary source. A total of 239 antibiotic resistance genes (ARGs) were identified in <em>P. mirabilis</em>, with β-lactamase and carbapenemase genes being particularly widespread and isolates from China harboring the highest ARG counts. Globally,<!--> <em>P. mirabilis</em> <!-->isolates were categorized into 17 distinct clusters, with U.S. isolates showing the widest phylogenetic spread. Minimal SNP variations among isolates from different countries and hosts suggest transnational and cross-host clonal propagation. Frequent clonal transmission was also observed among diverse hosts and clinical sources.<!--> <em>P. mirabilis</em> <!-->carries numerous integrative and conjugative elements (ICEs), some facilitating ARG dissemination (n = 215). Prophages, though ubiquitous, contributed minimally to ARG spread. Spearman’s analysis revealed significant correlations between ARGs and insertion sequences (ISs), replicons, and ICEs. Ancestral state analysis indicated prophages were mainly acquired horizontally, while other mobile genetic elements (MGEs) were largely clonally transmitted. This study provides the first comprehensive genomic analysis of<!--> <em>P. mirabilis</em>’s global resistance landscape, highlighting the need to designate it as a novel antimicrobial resistance indicator and implement long-term surveillance.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109487"},"PeriodicalIF":10.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of antibiotic-resistant pathogens in ancient deep-sea sediments predates anthropogenic antibiotic use","authors":"Zhitao Wu,&nbsp;Xiaobo Zhang","doi":"10.1016/j.envint.2025.109488","DOIUrl":"10.1016/j.envint.2025.109488","url":null,"abstract":"<div><div>The rapid escalation of antibiotic resistance of pathogenic bacteria has become a critical global public health threat. Despite the extensive use of antibiotics in medicine, debates on the origins of antibiotic-resistance pathogenic bacterium remain unresolved. In this study, five antibiotic-resistant pathogenic bacteria (<em>S. epidermidis</em>, <em>S. warneri</em>, <em>A. viridans</em>, <em>B. cereus</em> or <em>S. haemolyticus</em>), which could destroy the mouse intestines, were isolated from the deep-sea sediments with the geological ages of 8,008–23,931 years, indicating that the emergence of antibiotic-resistant pathogens predated the anthropogenic antibiotic use. The deep-sea pathogenic bacteria carried multiple antibiotic-resistant genes in their genomes, which exhibited the antibiotic resistance comparable to those of the current antibiotic-resistant pathogens. During the long history of coexistence with the antibiotic-producing bacterium in the deep sea, the deep-sea pathogenic bacteria evolved the resistance to antibiotics. The antibiotic-resistant pathogenic bacteria were distributed in the deep sea, the extreme ecosystem largely unaffected by human activities. In this context, our findings contributed novel insights into the natural origin and evolution of antibiotic-resistant pathogenic bacteria, providing a foundation for developing sustainable strategies to mitigate the global health challenge of antibiotic resistance.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109488"},"PeriodicalIF":10.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and mortality of acute aflatoxicosis: A systematic review","authors":"Tess Goessens ,&nbsp;Kokeb Tesfamariam ,&nbsp;Patrick Berka Njobeh ,&nbsp;Limbikani Matumba ,&nbsp;Nyadani Jali-Meleke ,&nbsp;Yun Yun Gong ,&nbsp;Zdenko Herceg ,&nbsp;Chibundu N. Ezekiel ,&nbsp;Sarah De Saeger ,&nbsp;Carl Lachat ,&nbsp;Marthe De Boevre","doi":"10.1016/j.envint.2025.109461","DOIUrl":"10.1016/j.envint.2025.109461","url":null,"abstract":"<div><h3>Background</h3><div>Aflatoxins are mycotoxins produced by <em>Aspergillus</em> fungi in crops intended for food and feed. Acute exposure to high levels of aflatoxin B1, one of the most toxic mycotoxins, can result in severe poisoning, defined as acute aflatoxicosis, which manifests as acute hepatic failure followed by death in severe cases. Currently global burden estimates of acute aflatoxicosis are lacking – in contrast to burden estimates of chronic exposure – making it difficult to implement and prioritize risk management strategies in the prevention and control of aflatoxin exposure.</div></div><div><h3>Aim</h3><div>This systematic review assessed global evidence on the incidence and mortality of acute aflatoxicosis from 1990 to 2023. While symptomology &amp; disease duration was also examined, it served as a secondary outcome to provide additional clinical context.</div></div><div><h3>Search Strategy and Eligibility</h3><div>A structured search was conducted in PubMed, Web of Science, Embase, Scopus, INASP and grey literature. Studies were imported into Covidence for review.</div></div><div><h3>Study Selection and Extraction</h3><div>Two independent reviewers screened and extracted titles, abstracts, and full texts. Eligible studies included all human studies.</div></div><div><h3>Results</h3><div>From 11,539 references, 9 studies were included. Heterogeneity existed in study design, region, age of the study population and aflatoxin analysis. Number of cases ranged from 1 to 317, with aflatoxin concentrations varying widely, <em>i.e.</em> between 10 and 51,100 µg/kg in food, 36 and 209,000 pg/mg albumin in serum, and 19 and 18,521 pg/g in tissue. Only one outbreak provided sufficient data to estimate an attack rate of 8 cases per 100,000. Mortality ranged from 16.2 to 76.5 %, affecting children under 15 and adults over 40 most severely. Common symptoms included vomiting (77–100 %), jaundice (88–100 %), and abdominal pain (8–87 %). The risk of bias was generally low.</div></div><div><h3>Conclusion</h3><div>This review shows that acute aflatoxicosis remains a significant public health burden, especially among vulnerable groups in African countries, although the variability in studies and lack of standardized reporting make burden estimation difficult, highlighting the need for better warning systems and standardized reporting, despite challenges with infrastructure and resources in affected areas.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109461"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics exposed by respiratory tract and exacerbation of community-acquired pneumonia: The potential influences of respiratory microbiota and inflammatory factors","authors":"Shaojie Liu ,&nbsp;Jianheng Zheng ,&nbsp;Wenbin Lan ,&nbsp;Zhiping Yang ,&nbsp;Meizhen Li ,&nbsp;Jing Li ,&nbsp;Jianguo Yu ,&nbsp;Shuyu Yang ,&nbsp;Jun Du ,&nbsp;Ruihua Dong ,&nbsp;Yihua Lin","doi":"10.1016/j.envint.2025.109485","DOIUrl":"10.1016/j.envint.2025.109485","url":null,"abstract":"<div><div>The relationships between microplastics (MP) exposure through respiratory and exacerbation of community-acquired pneumonia (CAP), as well as the potential influences of respiratory microbiota and inflammatory factors remain unknown in adults. Therefore, we conducted a cross-sectional study involving 50 non-severe CAP (NSCAP) and severe CAP (SCAP) patients to examine the associations of MP exposure in sputum (SP) and bronchoalveolar lavage fluid (BALF) samples with SCAP risk, and the underlying influences of respiratory microbiota and inflammatory factors. The average concentration of total MP was 23.24 μg/g dw and 4.49 μg/g dw in SP and BALF samples, with the detection rates of 98 % and 94 %. Participants who performing housework often or sedentary time ≤ 5h exhibited a higher proportion of high exposure to MP. Multivariable logistic regression and weighted quantile sum regression models showed the significantly positive relationships of single type or overall MP exposure with SCAP risk. Correlation analysis revealed that MP concentrations in BALF samples were significantly associated with multiple respiratory microbiota and inflammatory factors, particularly with the reduction in α-diversity indices of the respiratory microbiota. Our findings demonstrated that respiratory exposure to MP may cause the risk increase of SCAP, along with the alterations of respiratory microbiota and inflammatory factors. It is recommended that patients with CAP should reduce the respiratory exposure to MP for preventing the exacerbation of CAP in clinical practice.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109485"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deoxynivalenol exposure-related male reproductive toxicity in mammals: Molecular mechanisms, detoxification and future directions","authors":"Chongshan Dai ,&nbsp;Zhihui Hao ,&nbsp;Dingkuo Liu ,&nbsp;Zhanhui Wang ,&nbsp;Gea Oliveri Conti ,&nbsp;Tony Velkov ,&nbsp;Jianzhong Shen","doi":"10.1016/j.envint.2025.109478","DOIUrl":"10.1016/j.envint.2025.109478","url":null,"abstract":"<div><div>An increasing body of evidence indicates that exposure to widespread, environmental and food contaminants such as mycotoxins may cause endocrine disorders and infertility. Deoxynivalenol (DON), which is a toxic secondary metabolite produced by <em>Fusarium</em> fungi, can lead to multiple harmful effects in humans and animals, such as hepatotoxicity, nephrotoxicity, immunotoxicity, gastrointestinal toxicity, neurotoxicity, genetic toxicity and carcinogenicity. Recently, there has been growing concern about DON-induced male infertility. Exposure to DON and its metabolites can damage the structure and function of male reproductive organs, resulting in impairment of gametogenesis and thus impaired fertility. Potential molecular mechanisms involve oxidative stress, inflammatory response, mitochondrial dysfunction, apoptosis, cell cycle arrest, pyroptosis, and ferroptosis. Moreover, several signaling pathways, including nuclear factor-kappa B, mitogen−activated protein kinase, NLR family pyrin domain containing 3, nuclear factor erythroid 2-related factor 2, AMP-activated protein kinase, mitochondrial apoptotic pathways, and microRNAs are involved in these detrimental biological processes. Research has shown that several antioxidants, small-molecule inhibitors, or proteins (such as lactoferrin) supplementation can potentially offer protective effects by targeting these signaling pathways. This review comprehensively summarizes the harmful effects of DON exposure on male reproductive function in mammals, the underlying molecular mechanisms and emphasizes the potential of several small molecules as protective therapeutics. In the further, the systematic risk assessment when DON at environmental exposure doses to human reproductive health, the in-depth and precise molecular mechanism investigation using emerging technologies, and the development of more effective intervention strategies warrant urgent investigation.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109478"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive impact of the legislation on organotins contamination in the marine environment of Hong Kong","authors":"Yan Jiang ,&nbsp;Man Sze Kwok ,&nbsp;Huiju Lin ,&nbsp;Rainbow W.S. Leung ,&nbsp;Shaopeng Xu ,&nbsp;Juan C. Astudillo ,&nbsp;Mengyang Liu ,&nbsp;Kenneth M.Y. Leung","doi":"10.1016/j.envint.2025.109486","DOIUrl":"10.1016/j.envint.2025.109486","url":null,"abstract":"<div><div>Since September 2008, the use of organotin compounds (OTs) on antifouling systems on seagoing vessels has been globally banned by the International Maritime Organisation due to their toxic effects to non-target marine organisms. However, the regulation enforcement varies by government, hindering its effectiveness in controlling OTs contamination. For example, the Hong Kong Special Administrative Government enacted related legislation in January 2017. This study, conducted from 2022 to 2023, aimed to assess whether this law and its enforcement had significantly reduced OTs contamination in Hong Kong’s marine environment. The results showed significant reductions in concentrations of butyltins (BTs) in seawater and total OTs in the rock shell <em>Reishia clavigera</em> from 2010 to 2023, though only dibutyltin (DBT) decreased in sediment. Triphenyltin (TPT) was identified as the predominant compound in all matrices, with levels correlating positively with shipping activities. Imposex levels in <em>R. clavigera</em> also significantly decreased, as indicated by the vas deferens sequence index and the proportion of sterile female compared to 2010 and 2015. A probabilistic risk assessment based on tissue burden of tributyltin (TBT) and TPT in <em>R. clavigera</em> suggested that current TBT levels no longer had significant adverse effects on the gastropod, while TPT could impact 68% of their populations. Over a five-year period since the legislation, substantial reductions in BTs in coastal waters, and overall OTs in rock shells were observed, but TPT persisted at concerning levels. Continued risk management measures and regular monitoring are crucial to further mitigate TPT contamination in Hong Kong waters.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109486"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective role of 3-Indoleglyoxylic acid in Bisphenol S-induced intestinal barrier dysfunction via mitochondrial ROS-Mediated IL-17/CXCL10/TNF-α signaling","authors":"Jing Wang ,&nbsp;Guolei Niu ,&nbsp;Huanzhuo Mai ,&nbsp;Xianan Zhang ,&nbsp;Jiahao Zhu ,&nbsp;Bin Li ,&nbsp;Yushuang Gao ,&nbsp;Tengkai Huang ,&nbsp;Qingtao Meng ,&nbsp;Rui Chen","doi":"10.1016/j.envint.2025.109477","DOIUrl":"10.1016/j.envint.2025.109477","url":null,"abstract":"<div><div>Bisphenol S (BPS) has become extensively used in the manufacturing of consumer products. BPS mainly enters the body through food and water, with oral exposure targeting the gastrointestinal tract. However, its safety profile remains contentious and warrants further investigation. In this study, we aimed to assess whether BPS exerts harmful effects on the body in the absence of overt pathological damage. Our results revealed that although BPS did not lead to significant histopathological damage, it induced intestinal barrier dysfunction. Additionally, in vitro investigations utilizing NCM460 cells and human-derived colorectal organoids demonstrated that BPS exposure induced mitochondrial reactive oxygen species (ROS) levels in intestinal endocrine cells (EECs), upregulating the expression of inflammatory mediators TNF-α and CXCL10. Using a DSS-induced colitis mouse model, it was found that BPS exposure exacerbates the progression of intestinal inflammatory diseases. Analysis of single-cell databases demonstrated a significant reduction in the expression of <em>CHGA</em>, a functional protein of enteroendocrine cells (EECs), in patients with inflammatory bowel disease (IBD). The expression of <em>CHGA</em> showed a significant negative correlation with the expression of <em>IL17</em>. Notably, supplementation with 3-Indoleglyoxylic acid effectively mitigates the intestinal damage induced by BPS. These findings highlight the role of mitochondrial oxidative stress and IL-17/CXCL10/TNF-α signaling in BPS-induced intestinal damage and demonstrate the therapeutic potential of 3-Indoleglyoxylic acid in mitigating these effects.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109477"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traffic-related ultrafine particles influence gene regulation in olfactory mucosa cells altering PI3K/AKT signaling","authors":"Laura Mussalo ,&nbsp;Alexey M. Afonin ,&nbsp;Tana Zavodna ,&nbsp;Zdenek Krejcik ,&nbsp;Katerina Honkova ,&nbsp;Claire Fayad ,&nbsp;Muhammad Ali Shahbaz ,&nbsp;Juho Kalapudas ,&nbsp;Elina Penttilä ,&nbsp;Heikki Löppönen ,&nbsp;Anne M Koivisto ,&nbsp;Tarja Malm ,&nbsp;Jan Topinka ,&nbsp;Pasi Jalava ,&nbsp;Riikka Lampinen ,&nbsp;Katja M. Kanninen","doi":"10.1016/j.envint.2025.109484","DOIUrl":"10.1016/j.envint.2025.109484","url":null,"abstract":"<div><div>Traffic-related ultrafine particles (UFPs) are an emerging health concern affecting the brain and increasing the risk of Alzheimer’s disease (AD). PI3K/AKT signaling is known to contribute to neuronal survival and to be altered in AD. The nasal olfactory mucosa (OM) is a sensory tissue exposed directly to ambient air, and a starting point for olfactory neural circuits towards the brain. Evidence of air pollution-induced transcriptional regulation via microRNAs (miRNA) and DNA methylation (DNAmet) is accumulating and air pollutant-mediated disturbances in PI3K/AKT signaling have been reported. By utilizing a highly translational human-based <em>in vitro</em> model of OM, we aimed to investigate possible gene regulatory mechanisms in PI3K/AKT signaling induced by UFPs, and to compare the responses between cognitively healthy and individuals with AD. miRNA expression was analyzed using next-generation sequencing (NGS) and chip-based methylation analysis was performed to detect differentially methylated loci (DML). These data were combined with previously published transcriptomics analysis (mRNA) to construct an mRNA-miRNA-DNAmet-integrative network. Protein level changes were studied by immunoassays. We observed UFP-induced reductions in viability and increases in oxidative stress and DNA damage without eminent cell death. Integrative network analysis revealed multiple connections of miRNAs to differentially expressed genes in the PI3K/AKT pathway, and effects were most prominent in AD cells. Similarly, in AD cells DML were identified in transcription factor and apoptosis genes, downstream of PI3K/AKT signaling. Conclusively, traffic-related UFPs influence gene regulation of PI3K/AKT signaling to modulate OM cell survival, with existing AD pathology resulting in heightened vulnerability to UFP effects.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109484"},"PeriodicalIF":10.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}