Journal of Cell and Molecular Research最新文献_第7页

Biogeography of the Genus Linaria (Plantaginaceae) Based on Chromosome Number Data 基于染色体数目数据的车前草属生物地理学研究

Journal of Cell and Molecular Research Pub Date : 2015-12-26 DOI: 10.22067/JCMR.V7I2.41375

M. Ranjbar, S. Nouri

引用次数: 5

Investigating on the Stability of Peroxidase Extracted from Soybean (Glycine max var. Williams) and Effects of Na+ and K+ Ions on its Activity 大豆过氧化物酶(Glycine max var. Williams)的稳定性及Na+和K+离子对其活性的影响

Journal of Cell and Molecular Research Pub Date : 2015-12-26 DOI: 10.22067/JCMR.V7I2.43716

L. Parsiavash, A. Saboora, Seyed Mohsen Moosavi Nejad

{"title":"Investigating on the Stability of Peroxidase Extracted from Soybean (Glycine max var. Williams) and Effects of Na+ and K+ Ions on its Activity","authors":"L. Parsiavash, A. Saboora, Seyed Mohsen Moosavi Nejad","doi":"10.22067/JCMR.V7I2.43716","DOIUrl":"https://doi.org/10.22067/JCMR.V7I2.43716","url":null,"abstract":"In the present study, some techniques were used for Soybean peroxidase (SBP) purification including: ammonium sulfate fractionation, DEAE Sephadex anion exchange chromatography and Concanavalin A Sepharose 4B affinity chromatography. Molecular weight of purified SBP was estimated about 44 kDa by SDS-PAGE as a single polypeptide band. The optimal pH and temperature for enzyme activity were found to be 4.5 and 70◦C, respectively. The enzyme was more stable in alkaline pH than acidic ones and could tolerate 10 minutes heating in 40-50◦C without any loss of its activity. Both NaCl and KCl were found to have significant effects on the enzyme stability, but presence of NaCl was more effective than KCl. Our results showed that after 24 hours incubation of the enzyme in the presence of 20 mM NaCl, more than 60٪ of the enzyme activity was remained while it would fall to 3٪ if incubation was not accompanied by NaCl. Purified peroxidase from seed hull of soybean relative to the other identified peroxidases was more stable, for this reason a lot of benefit will be considered by use of this enzyme in different industry.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"94-101"},"PeriodicalIF":0.0,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

CXCR4: A Potential Chemokine Receptor for Future Regenerative Therapeutic Target CXCR4:未来再生治疗靶点的潜在趋化因子受体

Journal of Cell and Molecular Research Pub Date : 2015-12-26 DOI: 10.22067/JCMR.V7I2.52412

A. Heirani-Tabasi, H. Naderi-Meshkin

引用次数: 2

Warning of Reducing Genetic Diversity of Abramis brama (Berg, 1905) in Gilan Coast Using SSR Markers 利用SSR标记对吉兰海岸布兰(Berg, 1905)遗传多样性降低的警告

Journal of Cell and Molecular Research Pub Date : 2015-12-26 DOI: 10.22067/JCMR.V7I2.43997

Zeinab Hosseinnia, A. Shabani, H. K. Miandare

{"title":"Warning of Reducing Genetic Diversity of Abramis brama (Berg, 1905) in Gilan Coast Using SSR Markers","authors":"Zeinab Hosseinnia, A. Shabani, H. K. Miandare","doi":"10.22067/JCMR.V7I2.43997","DOIUrl":"https://doi.org/10.22067/JCMR.V7I2.43997","url":null,"abstract":"Genetic diversity is one of the three levels of biodiversity. The aim of present study was to compare levels of genetic polymorphism between wild Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions, the coast of Chamkhale and Bandaranzali of Gilan province. A total of seven microsatellite loci (MFW7, MFW26, Mcs1EH, Rser10, Bl1-153, Bl2-114 and IC654) were used. The average number of alleles in Chamkhale and Bandaranzali coast were 10 and 10.71 alleles, respectively. The numbers of effective alleles were 7.05 and 7.74 alleles in each population. Allele frequency was declined in wild fish due to inbreeding and genetic drift. The mean of observed heterozygosity values were 0.66 and 0.70 in Chamkhale and Bandaranzali coast, respectively. Approximately, all of loci showed deviation from Hardy-Weinberg equilibrium. The genetic similarity and distance between the two populations were 0.316 and 0.684, respectively. The results of Molecular Variance Analysis revealed that genetic diversity within locations was 97 percent, while among them was 3 percent. The Fst value was 0.024 that indicates the low genetic differentiation between the two locations which could be explained by the low number of alleles in two populations. Furthermore, the Natural Migration (Nm) between two stations was obtained 16.30. According to the analysis, it seems that Abramis brama has not a desirable genetic diversity in the investigated regions.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"108-114"},"PeriodicalIF":0.0,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Melanogenic Factors Gene Expression in Human Adult and Neonate Retinal Pigment Epithelium Cell Cultures 成人和新生儿视网膜色素上皮细胞培养中黑色素因子基因表达的研究

Journal of Cell and Molecular Research Pub Date : 2015-12-26 DOI: 10.22067/JCMR.V7I2.46456

A. Mohammadian, Z. Soheili, R. Jalal, A. Bagheri, S. Samiei, H. Ahmadieh

{"title":"Investigation of Melanogenic Factors Gene Expression in Human Adult and Neonate Retinal Pigment Epithelium Cell Cultures","authors":"A. Mohammadian, Z. Soheili, R. Jalal, A. Bagheri, S. Samiei, H. Ahmadieh","doi":"10.22067/JCMR.V7I2.46456","DOIUrl":"https://doi.org/10.22067/JCMR.V7I2.46456","url":null,"abstract":"Retinal pigment epithelium is responsible for maintaining the structural integrity of the retina by an efficient defense against free radicals, photo-oxidative exposure and light energy. For this purpose the main RPE line of defense is melanosomes. Melanin content of retinal pigment epithelium cells in adults and neonates reveals remarkable variations. In the current study we compared melanogenic factors gene expression levels in human adult and neonate RPE cells in culture. RPE cells from adult and neonate human eye globes were isolated and then cultured in DMEM: F12 (1:1) containing 10% FBS. Expression of RPE65 and Cytokeratin 8/18 markers in isolated cells was confirmed by immunocytochemistry. To evaluate the responsible factors in the pathway of melanin biosynthesis, gene expression of orthodenticle homeobox 2, microphthalmia-associated transcription factor A and H as prominent transcription factors, tyrosinase and dopachrome tautomerase as effective enzymes in the melanin biosynthetic pathway were examined in human neonate derived RPE cells compared to human adult derived RPE cells in culture. According to the Real-Time RT-PCR data, gene expression of MITF-H, OTX2, TYR and DCT in RPE cells of the neonates showed a significant increase compared to the adults. With increasing passage number, gene expression of MITF-H, OTX2, TYR and DCT showed remarkable decline. According to the role of OTX2 and MITF-H as the main transcription factor effectors on the TYR and DCT, restoration of OTX2 and MITF-H gene expression may be retain melanin content in RPE cells.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"76-85"},"PeriodicalIF":0.0,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Interactions of lncRNAs: Cellular Fate Determination and Tissue Regeneration lncrna的分子相互作用:细胞命运决定和组织再生

Journal of Cell and Molecular Research Pub Date : 2015-12-24 DOI: 10.22067/JCMR.V7I2.40861

M. Bahrami, Muhammad Irfan-maqsood

引用次数: 0

Characterization and Molecular Identification of Extracellular Polymeric Substance (EPS) Producing Bacteria from Activated Sludge 活性污泥胞外聚合物质(EPS)产菌的表征及分子鉴定

Journal of Cell and Molecular Research Pub Date : 2015-12-01 DOI: 10.22067/JCMR.V7I2.46198

Bahar Shahnavaz, M. Karrabi, Simin Maroof, M. Mashreghi

引用次数: 6

A Long noncoding RNA, ANCR, is Unregulated in Bladder and Breast Tumor Tissues 长链非编码RNA ANCR在膀胱和乳腺肿瘤组织中不受调控

Journal of Cell and Molecular Research Pub Date : 2015-06-30 DOI: 10.22067/JCMR.V7I1.37196

Mahshid Malakootian, Youssef Fouani, Parisa Naeli, F. Azad, S. Ziaee, S. Mowla

{"title":"A Long noncoding RNA, ANCR, is Unregulated in Bladder and Breast Tumor Tissues","authors":"Mahshid Malakootian, Youssef Fouani, Parisa Naeli, F. Azad, S. Ziaee, S. Mowla","doi":"10.22067/JCMR.V7I1.37196","DOIUrl":"https://doi.org/10.22067/JCMR.V7I1.37196","url":null,"abstract":"Long non-coding RNAs (lncRNAs) have recently found to have important regulatory roles, and their aberrant expressions and functions are directly linked to carcinogenesis. Both urinary bladder and breast tumors are prevalent neoplasms, with high rates of incidence. To identify a potential expression alteration of the recently discovered \"anti-differentiation non-coding RNA, (ANCR), during tumorigenesis, we initially assessed its expression in several cancer cell lines (LNCAP, MCF-7, Ht-29, 5637, A549, HepG2, and PC3) and then compared its expression variability in tumor vs. non-tumor samples of bladder and breast. Here, ANCR expression profile was studied by qRT-PCR in paired tumor and marginal non-tumor samples obtained from patients that had been referred to the Labbafi-Nejad and Imam Khomeini Hospitals, respectively. Our data revealed a significant upregulation (p = 0.003) of ANCR in breast tumor tissues, in comparison to non-tumor marginal specimens from same patients. Similar upregulation was also detected in bladder tumor samples, however, this alteration was not statistically significant (p ≥ 0.05), probably due to small number of samples (n = 10). In conclusion, our results suggest a possible role of ANCR in tumorigenesis of bladder and breast tissues, as well as its potential usefulness as a novel diagnostic biomarker for bladder and breast tumors.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Significance of Cell/Stem Cell Therapy in Wound Care Management 细胞/干细胞治疗在伤口护理管理中的意义

Journal of Cell and Molecular Research Pub Date : 2015-06-30 DOI: 10.22067/JCMR.V7I1.48006

Muhammad Irfan-maqsood

引用次数: 0

Application of Random Amplified Polymorphic DNA (RAPD) to Detect the Genotoxic Effect of Cadmium on Tow Iranian Ecotypes of cumin (Cuminum cyminum) 应用随机扩增多态性DNA (RAPD)检测镉对两种伊朗生态型孜然的遗传毒性效应

Journal of Cell and Molecular Research Pub Date : 2015-06-29 DOI: 10.22067/JCMR.V7I1.42174

Soraya Salarizadeh, H. Kavousi

{"title":"Application of Random Amplified Polymorphic DNA (RAPD) to Detect the Genotoxic Effect of Cadmium on Tow Iranian Ecotypes of cumin (Cuminum cyminum)","authors":"Soraya Salarizadeh, H. Kavousi","doi":"10.22067/JCMR.V7I1.42174","DOIUrl":"https://doi.org/10.22067/JCMR.V7I1.42174","url":null,"abstract":"Cadmium, a metal widely used in industrial processes, has been recognized to be a highly toxic and dangerous environmental pollutant. Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, two Iranian ecotypes of Cuminum cyminum (cumin) plantlets following exposure to cadmium (Cd) concentrations of 300–1050 µM for 7 days were screened for DNA genetic alterations by DNA fingerprinting. 10 RAPD primers of 50–70% GC content were found to produce unique polymorphic band profiles on treating cumin seedlings. After Cd treatment, significant changes were observed in RAPD profiles of both ecotypes. These changes included variation in band intensity, disappearance of bands, and appearance of new PCR products in comparison to the control group, and they were dose dependent. These results indicated that genomic template stability (GTS, a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentrations. The GTS index in both ecotypes gradually decreased with an increase in Cd concentrations. These findings suggest that DNA polymorphism detected by RAPD analysis could be a powerful eco-toxicological tool to evaluate the genotoxic effects of cadmium on plants.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"38-46"},"PeriodicalIF":0.0,"publicationDate":"2015-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13