Journal of Cell and Molecular Research最新文献

{"title":"Early Non-invasive Determination of Fetal Sex Using Cell-free DNA","authors":"S. Isakhani, A. Bahmanimehr","doi":"10.22067/JCMR.V8I2.57144","DOIUrl":"https://doi.org/10.22067/JCMR.V8I2.57144","url":null,"abstract":"The first step in the prenatal diagnosis of X-linked genetic disorders is determining fetus gender. Current invasive methods to obtain the DNA source of the fetus instead of its miscarriage risk, has harmful stress for high risk pregnancies. Cell free fetal DNA (cffDNA) circulating in the maternal blood, has now become a useful source of noninvasive prenatal diagnosis. Considering limitation of cffDNA; as its small fragment size and low concentration in maternal plasma; using this source for clinical diagnostic material, requires a high efficiency extraction method and reasonable molecular tests to lead more accurate results.In the current study, we optimized Triton/Heat/Phenol (THP) protocol for extracting cffDNA in 8 and 12 weeks gestation. Fetal sex determined for prenatal diagnosis of hemophilia using SRY gene markers and high resolution markers of sex chromosomes by QF-PCR. The results compared with genetic tests on CVS samples. We confirmed the persistence of fetal DNA in maternal blood and investigated cell-free fetal DNA as a reliable approach in prenatal diagnosis of hemophilia. High accuracy and possibility of analyzing circulating fetal DNA in maternal blood highlights this method as a reliable one to early non-invasive determination of fetal sex to avoiding problems of invasive methods","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"78-82"},"PeriodicalIF":0.0,"publicationDate":"2016-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometric Mean of 5S rRNA and MiR-16 As A Suitable Normalizer In Esophageal Cancer","authors":"S. Khazaei, S. Gharbi, S. Mowla","doi":"10.22067/JCMR.V8I2.56251","DOIUrl":"https://doi.org/10.22067/JCMR.V8I2.56251","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC) is a deadly cancer with poor prognosis. In this regard, early diagnosis is of vital importance to cure the tumor in its early stages. Novel cancer diagnostic and therapeutic approaches have been recently introduced based on microRNAs (miRNAs). Also, accurate normalization using appropriate reference genes is a critical step in miRNA expression studies. In this study, we aimed to identify appropriate reference genes for miRNA quantification in serum samples of ESCC. In this case and control experimental study, two statistical algorithms including GeNorm and NormFinder were used to evaluate the suitability of miR-16 and 5S rRNA and their geometric mean as reference genes. Then, relative expression of miR-451 and miR-24 were evaluated while different normalizer including miR-16, 5S rRNA and their geometric mean were applied. Both GeNorm and NormFinder analyses showed that geometric mean of miR-16 and 5S rRNA is the most stable reference gene in these samples. Also, our data showed that choosing an inappropriate normalizer could change the relative expression of target genes of miR-451 and miR-24 in ESCC samples which emphasize on the importance of selecting a reliable internal control in expression analyses. We demonstrated that geometric mean of two reference genes could increase the reliability of normalizers and also by using geometric mean as reference gene, relative expression of different target is closer to reality.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2016-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Chalcone Synthase and Chalcone Isomerase Gene Expression in Pigment Production Pathway at Different Flower Colors of Petunia Hybrida","authors":"F. Keykha, A. Bagheri, N. Moshtaghi","doi":"10.22067/JCMR.V8I1.50406","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.50406","url":null,"abstract":"Variegation in flower color is commonly observed in many plant species and also occurs on petunia (Petunia hybrida) \u0000as an ornamental plant. Variegated plants are highly valuable in the floricultural market. To gain a global perspective \u0000on genes differentially expressed in variegated petunia flowers, we investigated the expression of chalcone synthase \u0000(chs) and chalcone isomerase (chi) as two essential genes in biosynthesis pathway of pigment production. Also, we \u0000measured the concentration level of total flavonoids, naringenin chalcone and naringenin to evaluate the probably \u0000relationship between the expression profile of chs and chi genes and the concentration of mentioned pigments. The \u0000results indicated that chalcone synthase and chalcone isomerase expression had different profile in different petal \u0000color of Petunia hybrida. Because red flower color in petunia is related to the synthesis of pelargonidin-based (orange \u0000to red) pigments, our results suggest that the low chalcone synthase and chalcone isomerase expression levels in \u0000white petals reduce dihydrokaempferol formation, thereby inhibiting pelargonidin production. In contrast, the high \u0000expression levels of these genes observed in red petals ensure sufficient anthocyanin yields to make flowers red.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aegle marmelos Leaf Extract is an Effective Herbal Remedy in Reducing Hyperglycemic Condition: A Pre-clinical Study","authors":"A. K. Ch, M. Azam, A. Malik, Kalsoom Fatima, S. Jafri, R. Muhammad","doi":"10.22067/JCMR.V8I1.56204","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.56204","url":null,"abstract":"Diabetes is one of the alarming health problem in Pakistan. According to international diabetes federation (IDF), \u0000about 7 million Pakistanis are victims of diabetes accounting a total of 3% population. Most of Pakistanis still prefer \u0000to use herbal medicines for almost all of their health issues. Aegle marmelos (commonly known as the bael tree) is \u0000highly reputed ayurvedic medicinal tree as it has been used in traditional medicine for centuries. In this pre-clinical \u0000work, 32 mice were induced for diabetes via injecting alloxan intraperitoneally at a dose of 100 mg/kg body weight \u0000and mice having 104-170 mg/dl sugar level were considered diabetic. All mice were divided into two groups, each \u0000group with three sub-groups containing normal, control and diabetic mice. One group was given normal feed whereas \u0000other group was given a food containing Aegle marmelos leaf extract at 300 mg/kg body weight as an effective dose \u0000against alloxan induced hyperglycemia. The experiments lasted for 60 days and blood samples (05 ml to 1.0 ml) were \u0000collected from coccygeal vein of rats on 1st day, 30th day and 60th day of the experiment in heparinized tubes and \u0000blood glucose level was measured using spectrophotometer by enzymatic kit at a wavelength of 540nm. It was \u0000observed that the Aegle marmelos leaf extract is an effective herbal remedy in reducing and maintaining the glucose \u0000level in normal and hyperglycemic mice","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"39-45"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VARIATIONS IN PLASMA SEX STEROID HORMONES OF THE WILD CASPIAN CYPRINID FISH, KUTUM (RUTILUS FRISII KUTUM)","authors":"S. Saeed, Imanpoor Mohammad Reza, Aminian Fatideh Bagher, Gorgin Saeed","doi":"10.22067/JCMR.V8I1.44768","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.44768","url":null,"abstract":"Steroid hormones in plasma play an important role in reproductive cycles of animals especially during the final maturation stages. Steroid hormones synchronize gonad developments depending to fish species reproductive strategies. The wild Cyprinid fish, Kutum (Rutilus frisii kutum) is an ecologically and economically important fish species which inhabit in southern coastline of the Caspian Sea in Iran. Over the past few decades, natural reproduction of this species dramatically impaired due to the urbanization, civilization close to the land and shallow water in south western of the Caspian Sea. Therefore annual sex steroid hormones and gonads development were measured to assess the annual reproductive biology of female Kutum. In this study for the first time, our aims were to determine the annual variations in sex steroid hormones; 17 β Estradiol (E2), Progesterone (P) and testosterone (T) and gonad development of female Kutum. Our results showed that plasma steroid levels in females manifested in two phases in annual reproductive cycle; the resting phase (June - February) being characterized with the lowest level of steroid hormones and the peak reproduction activity phase (March–May) with simultaneously a significant increase in level of E2, P and T in plasma. Interestingly, comparing with other Teleost fish species the baseline level of E2 in plasma of Kutum during the resting phase to some extent was also huge. Increase in concentration of plasma E2 was in accordance with an increase of gonadosomatic index during spawning season. Our results contribute to our knowledge about the reproductive biology of Kutum and calls further long-term investigation.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"55 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus","authors":"Shiva Khezri, N. Bozorgi, F. Rahmani","doi":"10.22067/JCMR.V8I1.49336","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.49336","url":null,"abstract":"Multiple sclerosis is characterized by the loss of oligodendrocytes and demyelination of axons. In this study, the \u0000effect of caffeine on spatial memory in rats was investigated following demyelination induction by lysolecithin \u0000(LPC). The expression of Myelin Basic Protein (MBP), Glial Fibrillary Acidic Protein (GFAP), and Olig2 \u0000(oligodendrocyte lineage marker) genes was also assessed in the hippocampus. Animals were divided into seven \u0000groups; control group: animals received normal saline by stereotaxic intrahippocampal injection in Dentate Gyrus \u0000(DG) area; LPC group: animals received 2 μl lysolecithin by stereotaxic intrahippocampal injection in DG area (they \u0000were evaluated 7, 14, and 28 days after LPC injection; Caffeine- treated group: animals were treated with caffeine at \u0000doses of 30 mg/kg intraperitoneally for 7, 14, and 28 days after receiving LPC. Behavioral study was performed using \u0000Radial Arm Maze. Moreover, the RT- PCR was carried out for gene expression investigation. The demyelination and \u0000defective remyelination were noticeable on 28th day which suggests the demyelination decline caused by caffeine. \u0000Behavioral study showed that on the post-lesion days, the food finding time in the LPC group was significantly higher \u0000than that of the control group. Caffeine consumption significantly attenuated the food finding time in the treatment \u0000compared to the LPC group. The RT-PCR analysis indicated that the lysolecithin decreased the MBP expression \u0000especially on days 7 and 14 and conversely increased the Olig2 and GFAP expression. In addition, the caffeine \u0000enhanced the expression of MBP compared to that of the LPC group and reduced the Olig2 and GFAP expressions. \u0000Our results demonstrated that caffeine could increase the remyelination process in hippocampus and improve the \u0000spatial memory following demyelination induction by the LPC.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"15-24"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putative RFLP Analysis Between HSVd-sycv and Closely Related Variants","authors":"S. A. A. Bagherian","doi":"10.22067/JCMR.V8I1.50824","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.50824","url":null,"abstract":"Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed \u0000that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease. It was closely related to citrus variant \u0000of HSVd from Japan (HSVd-cit8) and with 93.7% homology with lime yellow corky vein variant of HSVd (HSVd– \u0000lycv). In this study, putative RFLP (Restriction Fragment Length Polymorphism) analysis using Vector NTI program \u0000(version 9.0.0) showed that some restriction enzymes could cut HSVd-sycv but not HSVd-lycv. On the other hand, \u0000M.Ngo BIX and FauI which could cut HSVd-lycv at positions of nt 53 and 97, respectively, could not cut HSVdsycv. \u0000Likewise, some restriction enzymes could cut HSVd-cit8 but could not cut HSVd-sycv. Therefore these variants \u0000can be recognized by these restriction enzymes.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"35-36"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of the Full-length Human Recombinant Keratinocyte Growth Factor in Pichia pastoris","authors":"Zahra Bahadori, S. Mowla, H. Kalhor","doi":"10.22067/JCMR.V8I1.50029","DOIUrl":"https://doi.org/10.22067/JCMR.V8I1.50029","url":null,"abstract":"Keratinocyte Growth Factor (KGF) is a paracrine-acting and epithelium-specific growth factor produced by cells of \u0000mesenchymal origin. Based on preclinical data, recombinant KGF plays a critical role in protecting and repairing of \u0000damaged epithelial tissues. Despite great efforts to express recombinant human KGF(rhKGF) in different organisms, \u0000attempts for finding appropriate protein expression system with the ability of producing a properly folded and \u0000processed KGF needs further investigation. Pichia pastoris has been used successfully and extensively for production \u0000of industrial enzymes and pharmaceutical proteins. Herein, we investigated the affect of pro-region-α-factor early \u0000deletion on production and secretion of rhKGF in Pichia pastoris. Initially, expression of human KGF induced in \u0000MCF-7 cell line treated with 1, 25-Dihydroxy vitamin D3. The coding sequence of full-length rhKGF194 was then \u0000cloned into the yeast integrative expression vector, downstream of α-factor and was integrated into P. pastoris \u0000genome. KGF protein was expressed in P. pastoris x33 cells, usingα-factor signal peptide for translocation of KGF \u0000to ER. An internal human signal peptide was also arranged after α-factorfor early removal of the pro-region in ER. \u0000RT-PCR results demonstrated that KGF mRNA was expressed successfully after induction by methanol. \u0000Recombinant KGF protein expression was detected by Western blotting in cell lysats, but not in conditioned media. \u0000A molecular weight of 17 kD for rhKGF194 indicates that the α-factor and internal human signal peptideshad been \u0000removed in x33 cells. The results indicate that in the absence of pro-region-α-factor, the recombinant KGF protein \u0000was not efficiently processed and transported within the biosynthesis-secretory pathway. As KGF protein is an \u0000unstable growth factor and tend to aggregate because of some native properties, It seems that presence of a chaperon \u0000molecule fusion with KGF is necessary for efficient secretion of the recombinant protein.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementary Analysis of Phosphoenolpyruvate Carboxykinase Gene Expression in Developing Seeds of Chickpea","authors":"M. Beihaghi, A. Bahrami, A. Bagheri, M. Z. Mehrjerdi","doi":"10.22067/JCMR.V7I2.42335","DOIUrl":"https://doi.org/10.22067/JCMR.V7I2.42335","url":null,"abstract":"Studies on the genes contributing to the seed filling in chickpea and its protein content might be valuable in engineering plants with seeds of a higher nutritional value. A gene of interest is phosphoenolpyruvate carboxykinase (pepck), encoding a protein with a substantial role in the gluconeogenesis pathway. In the present study, the protein content (percentage) was measured in a number of cultivated chickpea genotypes, followed by comparison of the expression levels of pepck gene at different stages of seed filling in some of the genotypes. This study aims at revealing the relation between pepck transcript level with protein content of chickpea seeds which might, in the longer term, end at protein quality improvement of the crop through the gene manipulation procedures. The results which were verified by Real Time-PCR and Western blot techniques in four genotypes of plant, showed that, the amount of pepck expression was significantly higher at the stage of seed fillingthan at other stages in all of genotypes and the lowest levels of expression belonged to flowering and seed formation and the PEPCK protein level was higher in the high protein genotypes compared to the low protein genotypes.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"102-107"},"PeriodicalIF":0.0,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Mesenchymal Stem Cells in Skin Wound Healing","authors":"Tahereh Sanjari, T. Hajjar, Madjid Momeni-Moghaddam","doi":"10.22067/JCMR.V7I2.51937","DOIUrl":"https://doi.org/10.22067/JCMR.V7I2.51937","url":null,"abstract":"Mesenchymal stem cells (MSCs) could differentiate into various types of tissues. These cells serve as a backup for the regeneration and repair of tissues or cells after injury. A skin wound is defined as an injury to the skin that needs to be restored. All types of cells in skin especially mesenchymal stem cells play important role in wound healing process. In particular, paracrine signaling of MSC regulates the cellular responses at the wound site leading to reduction of inflammation, stimulation of angiogenesis and induction of cell migration and proliferation. Because of these abilities, MSCs are one of the most common stem cells for cell therapy in wound healing. This review focuses on the role of MSCs on wound healing process. In addition, major phases of wound repair and challenges of cell therapy are discussed.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"70-75"},"PeriodicalIF":0.0,"publicationDate":"2015-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}