Journal of Cell and Molecular Research最新文献_第10页

First five years of scientific publications in JCMR / The Editorial 前五年在JCMR / The Editorial上发表的科学出版物

Journal of Cell and Molecular Research Pub Date : 2014-06-28 DOI: 10.22067/JCMR.V6I1.30629

Muhammad Irfan-maqsood

引用次数: 0

Karyological study of the Caspian bent-toed Gecko Cyrtopodion caspium (Sauria: Gekkonidae) from North and North-Eastern of Iran 伊朗北部和东北部里海弯趾壁虎(亚纲:弯趾壁虎科)的核学研究

Journal of Cell and Molecular Research Pub Date : 2014-06-25 DOI: 10.22067/jcmr.v6i1.31318

F. Molavi, Haji GholiKami, M. Yazdanpanahi

{"title":"Karyological study of the Caspian bent-toed Gecko Cyrtopodion caspium (Sauria: Gekkonidae) from North and North-Eastern of Iran","authors":"F. Molavi, Haji GholiKami, M. Yazdanpanahi","doi":"10.22067/jcmr.v6i1.31318","DOIUrl":"https://doi.org/10.22067/jcmr.v6i1.31318","url":null,"abstract":"The diploid number among Gekkonid lizards ranges from 2n=16 to 2n=46. The majority of geckos' chromosomes are small which makes the study of their chromosomes very difficult. For this reason, karyotype of Cyrtopodion caspium and some similar gecko are still not known. The close relatives of C. caspium have variation in the chromosomal number in their populations. So to find out possible variability among these populations in Iran. We described the karyotype of testis and bone marrow of 14 specimens of this species from seven provinces and 10 localities in north and north-eastern parts of Iran in 2011. They showed 2n=38 for all populations. There is not intraspecific chromosome polymorphism in Cyrtopodion caspium. The karyotype consists of one pair of metacentric element and 17 pairs of telocentric or subtelocentric elements and one pair of three arm chromosomes. The number of haploid chromosomes was 21 for three male specimens. The chromatid separation did not show heteromorphic sex chromosomes. Our results highlight the need for continued research into the basic biology and taxonomy of Cyrtopodion caspium in Iran.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2014-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PAX6 (+5a) Expression in Adipose Tissue-Derived Stem Cells Induces Retinal Ganglion Cells PAX6 (+5a)在脂肪组织源性干细胞中的表达诱导视网膜神经节细胞

Journal of Cell and Molecular Research Pub Date : 2014-03-12 DOI: 10.22067/JCMR.V6I1.30448

Habib Rezanejadbardeji, F. Haddad, Z. Soheili, M. Matin, S. Samiei, S. Z. Vahed

{"title":"PAX6 (+5a) Expression in Adipose Tissue-Derived Stem Cells Induces Retinal Ganglion Cells","authors":"Habib Rezanejadbardeji, F. Haddad, Z. Soheili, M. Matin, S. Samiei, S. Z. Vahed","doi":"10.22067/JCMR.V6I1.30448","DOIUrl":"https://doi.org/10.22067/JCMR.V6I1.30448","url":null,"abstract":"Glaucoma remains one of the major causes of blindness in today's world. The progressive field of stem cell proposes an exciting potential for discovering novel therapies. Here, we report the development of an easy and high throughput method for differentiation of retinal ganglion cells (RGC) and bipolar cells from human adipose tissue-derived mesenchymal stem cells (hADSCs) using PAX6 (+5a) gene expression, a master gene in development of the vertebrate visual system. HADSCs was isolated from fat tissues and confirmed by their surface markers and differentiation potential into adipocytes and osteocytes lineages. Then, the coding region of human PAX6 (+5a) gene was cloned and lentiviral particles were produced. HADSCs differentiation was characterized by morphological characteristics, qRT-PCR and immunocytochemistry (ICC). The hADSCs were isolated successfully with high yield and purity (99%). After 30 hours post transduction by pLEX-pax6- pur lentiviral vectors in fibronectin supplemented medium, cells gradually showed the characteristic morphology of neuronal cells. QRT- PCR and ICC confirmed deriving of mainly RGC and marginally bipolar cells. The current investigation demonstrates the feasibility of differentiation of RGCs and bipolar cells from hADSCs using expression of PAX6 (+5a) in the medium supplemented by fibronectin.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"3-12"},"PeriodicalIF":0.0,"publicationDate":"2014-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Application of Organotypic Brain Slice Culture to Study Microglial Differentiation by Lycopersicon esculentum and Sambucus nigra Lectin Histochemistry 应用器官型脑切片培养研究番茄和黑参凝集素对小胶质细胞分化的组织化学作用

Journal of Cell and Molecular Research Pub Date : 2014-01-01 DOI: 10.22067/JCMR.V6I2.38121

Roya Lari, P. Kitchener

{"title":"The Application of Organotypic Brain Slice Culture to Study Microglial Differentiation by Lycopersicon esculentum and Sambucus nigra Lectin Histochemistry","authors":"Roya Lari, P. Kitchener","doi":"10.22067/JCMR.V6I2.38121","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.38121","url":null,"abstract":"Microglial cells are the subset of macrophages in the central nervous system (CNS). Changes in the CNS such as injury or developmental events cause morphological and physiological changes in microglial cells. In this study organotypic brain slice cultures under serum free condition were used to investigate the morphology and lectin histochemistry of microglia and macrophages in the CNS in vitro. Microglial cells exhibited dramatic morphological changes in the organotypic brain slice culture. Immediately after slicing microglias were seen to have the same morphology as they do in the intact brain: they had small cell bodies from which radiated several highly ramified processes. After 1 day in vitro (DIV) all microglia transformed into an active form with round soma and no processes. At 5 days in vitro, and especially at 9 days in vitro, many of the microglia had tended to return to the ramified phenotype. The expression of different carbohydrates was examined at the 0, 1, 5 and 9 days in vitro time periods by employing Lycopersicon esculentum tomato lectin (LEA lectin) and Sambucus nigra (SNA). Microglial cells with different morphology intensely stained with the LEA. SNA stained the ramified microglia only after they re-ramified at 5 DIV and 9 DIV. The results of this study confirmed that the expression of carbohydrate structures in these cells would undergo changes corresponding to the changes in morphology.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic polymorphism of K-casein, β-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle 伊朗荷斯坦牛k -酪蛋白、β-乳球蛋白和瘦素基因多态性及其与产奶量性状的关系

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.21086

Y. Mohammadi, A. Aslaminejad, M. Nassiry, Ali Esmailizadeh Koshkoieh

{"title":"Allelic polymorphism of K-casein, β-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle","authors":"Y. Mohammadi, A. Aslaminejad, M. Nassiry, Ali Esmailizadeh Koshkoieh","doi":"10.22067/JCMR.V5I2.21086","DOIUrl":"https://doi.org/10.22067/JCMR.V5I2.21086","url":null,"abstract":"The purpose of this study was to investigate the polymorphism of K-casein (K-CN), β-Lactoglobulin (B-LG) and leptin (LP) genes in Iranian Holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. DNA was extracted from blood samples of 139 cows using a modified phenol chloroform method. Association between K-CN, B-LG and LP genes’ polymorphism with milk production traits were investigated using mixed procedure of SAS software. The frequencies of AA, AB and BB genotypes for K-CN (0.72, 0.18 and 0.10), B-LG (0.43, 0.28 and 0.29) and LP (0.24, 0.63 and 0.13) were also calculated. Statistical results revealed a significant association between AA and BB genotypes of the K-CN gene with milk production and milk protein percentage, respectively. Also, BB genotype of the B-LG gene and AA genotype of the LP gene showed a significant association with protein percentage and milk production (P<0.05), respectively. Therefore, it is feasible to improve milk production traits in these herds using the studied genes.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"5 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68590611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Therapeutic potential of Stem Cell Preconditioning for Ischemic Heart Diseases / Letter to the Editor 干细胞预处理对缺血性心脏病的治疗潜力/致编辑的信

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.29470

M. Mirahmadi, A. Heirani-Tabasi, H. Hassanzadeh, M. Pishbin, H. Bidkhori, H. Naderi-Meshkin

引用次数: 2

Developments toward an Ideal Skin Substitute: A Commentary 向理想皮肤替代品的发展:评论

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.29491

Muhammad Irfan-maqsood, S. Sadeghi

引用次数: 5

In silico analysis of chimeric recombinant immunogen against three diarrhea causing bacteria 三种致泻菌嵌合重组免疫原的硅片分析

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.22187

Farzaneh Khaloiee, Poneh Pourfarzam, I. Rasooli, J. Amani, S. Nazarian, S. Mousavi

{"title":"In silico analysis of chimeric recombinant immunogen against three diarrhea causing bacteria","authors":"Farzaneh Khaloiee, Poneh Pourfarzam, I. Rasooli, J. Amani, S. Nazarian, S. Mousavi","doi":"10.22067/JCMR.V5I2.22187","DOIUrl":"https://doi.org/10.22067/JCMR.V5I2.22187","url":null,"abstract":"Shigella and Escherichia belong to the Enterobacteriaceae family which are the cause for most of the diarrheal cases in the world. Shigella can cause bacterial dysenteries and shigellosis. One of the most effective proteins for pathogenesis is invasion plasmid antigen C (IpaC). Other bacteria like Enterotoxogenic (ETEC), Enterohemorrhagic (EHEC), and E.coli can also cause diarrhea and produce intestinal disorders. Colonization factor antigen I (CFA/I), a critical virulence protein for these infections, has two subunits i.e. CfaB and CfaE. EHEC Attachment of bacteria is the main step of infection with intimin playing the key role in this function. This study was designed to elicit protection against the majority of diarrheal pathogens via development of polyvalent vaccine against Shigella, ETEC and EHEC. In silico techniques are as best tools to design new vaccines. For this purpose the immunogenic epitopes of CfaB, IpaC and Intimin were identified through bioinformatic tools and were then selected as major antigens to construct a chimeric protein (CII). The humoral and cellular immunities were analyzed bioinformatically. Prediction of allergens and mapping of IgE epitopes were carried out. The bioinformatic analysis showed each domain was folded separately in fusion structure. CII had many T and B cell epitopes in both linear and three-dimensional structures. This prediction of the chimeric construct had the potential to induce CD4+ and CD8+ immune responses against these pathogens. In addition CII could be accessible to surveillance by the immune system in mouse and human. In conclusion, in silico analysis showed that this chimeric protein can be used as a vaccine against Shigella, ETEC and EHEC simultaneously.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"5 1","pages":"65-74"},"PeriodicalIF":0.0,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Assessment of genetic stability of olive in vitro propagated by RAPD marker RAPD标记对橄榄离体繁殖遗传稳定性的评价

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.24679

M. Peyvandi, M. Monsef, M. H. Mazinani

引用次数: 5

The significance of C-terminal NLS sequences of VirD2 in its nuclear localization in Saccharomyces cerevisiae 酿酒酵母VirD2 c端NLS序列在其核定位中的意义

Journal of Cell and Molecular Research Pub Date : 2013-12-29 DOI: 10.22067/JCMR.V5I2.13541

J. Soltani, J. A. Lal, G. Heusden, P. Hooykaas

{"title":"The significance of C-terminal NLS sequences of VirD2 in its nuclear localization in Saccharomyces cerevisiae","authors":"J. Soltani, J. A. Lal, G. Heusden, P. Hooykaas","doi":"10.22067/JCMR.V5I2.13541","DOIUrl":"https://doi.org/10.22067/JCMR.V5I2.13541","url":null,"abstract":"Agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. Indeed, upon infection of eukaryotic cells, Agrobacterium tumefaciens transfers a piece of its tumor inducing (Ti)-plasmid, called T-DNA, to the host cell nucleus, which subsequently integrates into the host genome. The VirD2 virulence protein which has relaxase endonuclease activities covalently binds to the 5'end of T-DNA and facilitates its transfer, nuclear localization and integration into the host genome in collaboration with the interacting proteins of the host cell. The VirD2 is essential for Agrobacterium–mediated transformation of both plants and non-plant cells. Here, using yeast Green Flourescent Protein (yGFP) technology, we studied the subcellular localization of VirD2, expressed in the model eukaryote Saccharomyces cerevisiae. Fluorescence microscopy showed that an N-terminal yGFP fusion of VirD2 (i.e. 5' GFP-VirD2 3'), was located in the nucleus of yeast. With C-terminal fusions of VirD2 to yGFP (i.e. 5' VirD2-GFP 3'), no particular subcellular concentration of fluorescence was seen. This further confirms nuclear localization of VirD2 in eukaryotic cells and more importantly highlights the role of Nuclear Localization Signal sequences (NLS) of the C-terminal of VirD2 in this phenomenon.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"5 1","pages":"60-64"},"PeriodicalIF":0.0,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68590523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0