Journal of Cell and Molecular Research最新文献

Effect of glutamine stability on the long-term culture and line establishment of chicken primordial germ cells 谷氨酰胺稳定性对鸡原始生殖细胞长期培养和系建立的影响

Journal of Cell and Molecular Research Pub Date : 2021-09-14 DOI: 10.22067/JCMR.2021.71863.1017

Sara Yousefi Taemeh, J. Mehrzad, Hesam Dehghani

{"title":"Effect of glutamine stability on the long-term culture and line establishment of chicken primordial germ cells","authors":"Sara Yousefi Taemeh, J. Mehrzad, Hesam Dehghani","doi":"10.22067/JCMR.2021.71863.1017","DOIUrl":"https://doi.org/10.22067/JCMR.2021.71863.1017","url":null,"abstract":"Primordial germ cells (PGCs) are precursors for mature gametes, which transmit genetic information to the next generation. Due to the importance of PGCs in many fields including developmental biology, genome editing, transgenesis, and conservation of avian genetic resources, a lot of research has focused on the cultivation of PGCs. Despite considerable progress in the establishment of specified culture media for the expansion of PGCs, a well-defined PGC culture medium has not yet been developed. This might be due to the complexity of the nutritional requirements of PGCs in the culture. Besides the nutritional needs including vitamins, amino acids, salts, carbohydrates, and growth factors, a certain source of energy must be provided to sustain growth and viability. Glutamine is a major energy source for cultured cells, which is commonly added in cell culture media at higher concentrations than other amino acids. However, glutamine is very labile and rapidly degrades in solutions such as culture media. This generates ammonia as a by-product, which is toxic to the cultured cells and can affect cell viability and protein glycosylation. Therefore, the stability of glutamine in culture conditions is another concern for the long-termed culture of PGCs. Here, we study the effect of glutamine stability on PGC culture using glutamine and GlutaMax (a commercial stabilized dipeptide form of glutamine). We found that the addition of GlutaMax in the medium promotes PGC proliferation. This effect might be exerted by minimizing toxic ammonia buildup that results in maximizing cell performance, and also increasing media stability.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44190434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Understanding the Effect of Natural Products on Breast Cancer via P53-MDM 2 Signal Pathway 通过P53-MDM 2信号通路了解天然产物对乳腺癌的影响

Journal of Cell and Molecular Research Pub Date : 2021-09-01 DOI: 10.22067/JCMR.2021.71513.1012

Zahra Ghavidel, M. Moghaddam, T. Hajjar, Eisa Kohan-Baghkheirati

{"title":"Understanding the Effect of Natural Products on Breast Cancer via P53-MDM 2 Signal Pathway","authors":"Zahra Ghavidel, M. Moghaddam, T. Hajjar, Eisa Kohan-Baghkheirati","doi":"10.22067/JCMR.2021.71513.1012","DOIUrl":"https://doi.org/10.22067/JCMR.2021.71513.1012","url":null,"abstract":"The use of medicinal plants in the treatment of diseases has a long history dating back to the presence of humans on earth. Cancer has almost been an incurable disease, and among the various cancers, breast cancer is the most common type of cancer among women and imposes an enormous burden on patients. Although medical and surgical solutions have been proposed for the disease, it has not been successful enough to treat the disease in many patients. In recent years, more studies have been done on the effects of medicinal plants on breast cancer, and scientists are trying to find the exact mechanisms of action for these plants to find effective ways for controlling cancer cell growth. This article focuses on P53 and MDM2, two very important genes involved in regulation of cell growth and proliferation both in cancer and normal tissue, and we also gathered the list of natural compounds targeting the MDM2-p53 pathway. Our results provide a list of plant families that can influence this pathway and have great potential in designing treatments against cancers that encompass deregulation of the MDM2-p53 pathway.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"13 1","pages":"72-80"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45875616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Multi-Faceted Approach for Prediction of Genome Safe Harbor Loci in the Chicken Genome 鸡基因组安全港基因座的多面预测方法

Journal of Cell and Molecular Research Pub Date : 2021-08-23 DOI: 10.22067/JCMR.2021.71604.1015

N. Dehdilani, Sara Yousefi Taemeh, Lena Goshayeshi, M. Najafi, Hesam Dehghani

{"title":"A Multi-Faceted Approach for Prediction of Genome Safe Harbor Loci in the Chicken Genome","authors":"N. Dehdilani, Sara Yousefi Taemeh, Lena Goshayeshi, M. Najafi, Hesam Dehghani","doi":"10.22067/JCMR.2021.71604.1015","DOIUrl":"https://doi.org/10.22067/JCMR.2021.71604.1015","url":null,"abstract":"To achieve a reliable and persistent expression over time, the transgene should be precisely integrated into the genome safe harbor (GSH) loci. Little attention has been paid to find the safe harbor loci of the chicken (Gallus gallus domesticus) genome. Identification and characterization of GSH loci that allow the persistent and reliable expression of knocked-in genes could be a major area of interest within the field of transgenic technology and central to the development of bioreactor transgenic livestock. Randomly integrated transgenes might be encountered position effects and epigenetic silencing, so unstable phenotypes as well as unreliable and unpredictable expression of knocked-in transgene could be occurred. In contrast to random gene insertion, site-specific gene targeting provides a specific strategy that exploits homologous recombination to insert a transgene of interest into a pre-determined locus, specifically.In this study, using bioinformatics, gene expression atlas, and Hi-C data, the GSH region between drg1 and eif4enif1 genes was predicted in the chicken genome. Contrary to the previous time-consuming and expensive methods, here we introduce a fast and easy-to-use pipeline allowing the prediction of orthologue GSH loci in all organisms, especially in chicken. Then, the standard design of targeting and gRNA/Cas9 vectors is explained in detail.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48251339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vitamin C Loaded Albumin Nanoparticles Treatment and Its Effect on Collagen I and III and mir-133 Genes Expression 负载维生素C的白蛋白纳米颗粒处理及其对胶原I、III和mir-133基因表达的影响

Journal of Cell and Molecular Research Pub Date : 2021-08-06 DOI: 10.22067/JCMR.2021.71443.1011

Hamid Reza Shojania, M. Moghaddam, S. Hossini

{"title":"Vitamin C Loaded Albumin Nanoparticles Treatment and Its Effect on Collagen I and III and mir-133 Genes Expression","authors":"Hamid Reza Shojania, M. Moghaddam, S. Hossini","doi":"10.22067/JCMR.2021.71443.1011","DOIUrl":"https://doi.org/10.22067/JCMR.2021.71443.1011","url":null,"abstract":"Wound healing is a complex biological process in which many molecules, including microRNA molecules, play an important role in its regulation. It is well established that reducing mir-155 expression can accelerate wound healing. In this study, the effect of using nanoparticle loaded by vitamin C in wound healing and mir-133b, collagen I and III expression changes was investigated. In this study, first nanoparticles of albumin protein were produced and then loaded with vitamin C. to evaluate the toxicity and determine the appropriate dose, these nanoparticles were affected on fibroblast cell and cell behavior was investigated. Also in the molecular study, changes in the expression of collagen I and III genes were studied. The results show that nanoparticles containing vitamin C had a positive effect on the expression of collagen I and III compared to the control group. Also we observed a decrease in the expression of mir-133 compared to the control group. Therefore, the results of this study clearly indicate the role of nanoparticles in improving the expression of collagen I and III genes, also the expression of mir-133 gene is reduced, which leads to the activation of collagen genes I and III, which these findings favor the high expression of collagen by fibroblasts, which ultimately have a positive effect on wound healing.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43987777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Commercial CCL21 and CCL21/IL1β Recombinant Protein by In Silico Tools 商用CCL21与CCL21/ il - 1β重组蛋白的比较分析

Journal of Cell and Molecular Research Pub Date : 2021-07-22 DOI: 10.22067/JCMR.2021.69999.1005

Ahdiyeh Shahtaghi, Ali Alam Shahnabadi, M. Beihaghi, Kamelia Kohannezhad, N. Amini

{"title":"Comparative Analysis of Commercial CCL21 and CCL21/IL1β Recombinant Protein by In Silico Tools","authors":"Ahdiyeh Shahtaghi, Ali Alam Shahnabadi, M. Beihaghi, Kamelia Kohannezhad, N. Amini","doi":"10.22067/JCMR.2021.69999.1005","DOIUrl":"https://doi.org/10.22067/JCMR.2021.69999.1005","url":null,"abstract":"One of the newest diagnostic methods and treatment of cancer is to design new drugs. It is now possible to design a drug with desired properties in theory and evaluate its therapeutic effects through bioinformatics tools. Among the studied drugs; those based on cytokine genes, which increase the body's immunity against cancer, are of great interest. Cytokines are small proteins that play an important role in cell signaling and can affect the function and behavior of surrounding cells. CCL21 chemokine is one of the cytokines that possess antitumor properties has potential on chemoa-ttraction of T lymphocytes and dendritic cells. Interleukin 1 beta (IL1β) is a cytokine involving in different cellular activities such as activation of neutrophils, B-Cells and T-Cells. In the present study, we designed a drug-based cytokine gene to activate T cells and B cells by inserting defined CCL21 epitope and IL1β pepteide sequences into a protein construct. Molecular dynamics simulation was performed in Linux space using Gromex software. Results of RMSD, RMSF and the radius of gyration obtained from the simulation showed the stability of both proteins which indicated that there are no significant conformational differences between the commercial CCL21 and recombinant form. The interaction of synthetic construct and human CCL21 with CCR7 receptor was also investigated by HADDOCK software. Obtained results showed that there are no differences between these proteins and recombinant protein has the same structural and conformational characteristics as human commercial CCL21.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48698471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells 王浆降低人膀胱癌症5637细胞MMP-9表达并诱导细胞凋亡

Journal of Cell and Molecular Research Pub Date : 2021-07-09 DOI: 10.22067/JCMR.2021.68523.1000

Narges Fazili, Z. Soheili, S. Malekzadeh-Shafaroudi, S. Samiei, N. Moshtaghi, A. Bagheri

{"title":"Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells","authors":"Narges Fazili, Z. Soheili, S. Malekzadeh-Shafaroudi, S. Samiei, N. Moshtaghi, A. Bagheri","doi":"10.22067/JCMR.2021.68523.1000","DOIUrl":"https://doi.org/10.22067/JCMR.2021.68523.1000","url":null,"abstract":"Royal jelly (RJ) from queen honeybee larva as a traditional medicine agent has a variety of pharmacological benefits. In the present study, the effect of Royal jelly was investigated on the urinary bladder cancer cell line (HTB-9 5637). To determine the cell viability in different concentrations of Royal jelly, MTT assay was performed. An in vitro wound healing assay was applied to investigate the effect of RJ on cell migration. The activity and gene expression level of matrix metalloproteinase 2 and 9 was assessed by zymography and Real time PCR respectively. R.J.S at the concentration of 0.7 mg/ml had a significant effect on reducing the proliferation rate of 5637 cells after 72h (p < 0.009). R.J.S significantly decreased cell migration and induced a significant decrease in the transcriptional level of MMP9 after 72h (0.5x; P < 0.049). However R.J.S did not impose any effect on the expression level and activity of matrix metalloproteinase 2. These results indicate the potential of RJ as a promised natural anti-proliferative and anti-metastatic drug in combination with advanced therapy methods for cancer treatment. Royal jelly has the potential to be more focused as an anti-metastatic drug to control tumor growth and can be considered as a more effective alternative to the current chemotherapy drugs.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45494689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioassay and Molecular Screening of Nitrate Reductase Enzymes in halophilic bacteria 嗜盐细菌硝酸还原酶的生物测定及分子筛选

Journal of Cell and Molecular Research Pub Date : 2021-05-05 DOI: 10.22067/JCMR.2021.64301.0

Saeedeh Sibevieh, E. Salehghamari, M. A. Amoozegar, M. Zolfaghari, M. Soleimani, Zohre Nasrollahzadeh, Sara Eftekhari Yazdi

{"title":"Bioassay and Molecular Screening of Nitrate Reductase Enzymes in halophilic bacteria","authors":"Saeedeh Sibevieh, E. Salehghamari, M. A. Amoozegar, M. Zolfaghari, M. Soleimani, Zohre Nasrollahzadeh, Sara Eftekhari Yazdi","doi":"10.22067/JCMR.2021.64301.0","DOIUrl":"https://doi.org/10.22067/JCMR.2021.64301.0","url":null,"abstract":"With the increased usage of nitrate fertilizers, as it is a stable ion with high solubility in water, its removal is a challenge for human health. Several physicochemical methods have been examined for nitrate removal of water, but nowadays the biological treatment of it from soil and water are widely used because of its high efficiency and low cost. To remove nitrogen from such environments, we studied nitrate reducing halophilic and halotolerant bacteria. A total of 50 strains of halophilic and halotolerant bacteria from different saline and hypersaline environments of Iran, including Incheboron wetland, Aran-Bidgol Lake and Urmia Lake, were screened for nitrate reductase production. Among isolated bacteria, 60% of strains isolated from Urmia Lake and 19% of strains isolated from Incheboron wetland produced nitrate reductase. Nitrate reductase coding genes narG and napA were analyzed for all the nitrate reducing strains. The napA gene was amplified successfully from a gram negative halophilic strain and narG gene was detected in ten halophilic strains. Among nitrate reducing isolates, Kocuria rosea strain R3A34 which has narG gene showed the most nitrate reductase production. The strain R3A34 was selected for optimization of denitrifying activity. Statistical results have shown that the optimum conditions for the production of nitrate reductase were 32 °C, pH 7.0, NaCl 8 % (w/v) and mannitol as carbon source. Furthermore, as these conditions are common in wastewaters, this bacterium can be a proper candidate for bioremediation of wastewaters from nitrate pollutants.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42404471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Anticancer Efficacy of Mixture of Local Probiotics on MDA-MB-231 and MCF-7 Breast Cell Lines 局部益生菌混合物对MDA-MB-231和MCF-7乳腺细胞系的抗癌作用探讨

Journal of Cell and Molecular Research Pub Date : 2021-04-27 DOI: 10.22067/JCMR.2021.68651.1001

M. Abdoli, P. Rezaei, K. Mansouri

{"title":"Exploring the Anticancer Efficacy of Mixture of Local Probiotics on MDA-MB-231 and MCF-7 Breast Cell Lines","authors":"M. Abdoli, P. Rezaei, K. Mansouri","doi":"10.22067/JCMR.2021.68651.1001","DOIUrl":"https://doi.org/10.22067/JCMR.2021.68651.1001","url":null,"abstract":"The aim of this study was to investigate the cytotoxicity of probiotic mixture on breast cancer cell lines. A mixture of local probiotic bacteria was cultured and the supernatant was lyophilized for performing additional experiments. The antioxidant activity, total phenol content (TPC) and fatty acid composition of bacterial supernatant (BS) were also measured. The effect of probiotic mixture on cell viability of two breast cancer cell lines and apoptosis induction were investigated using MTT assay and DAPI staining, respectively. The highest antioxidant activity and total phenol content (TPC) of the bacterial supernatant (BS) were detected at 3200 µg/ml. According to the GC–MS analyses, linoleic acid (37.40 %) and oleic acid (26.93 %) were identiﬁed as the major fatty acids of the BS. The MTT assay showed that BS has antiproliferative effect on MDA-MB-231 and MCF-7 cells in a time and dose-dependent manner, and also the 3200 μg/ml was determined as IC50. The apoptosis induction was confirmed by morphological characteristics on both cell lines, including nucleus changes, granulation of the nucleus, and the formation of the apoptotic bodies. According to this experiment, cytotoxic activity and apoptosis potential of BS on two breast cancer cell lines including MDA-MB-231 and MCF-7 was shown. BS could be considered as a suitable anticancer agent","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47176903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer LncRNA HOXD-AS1在卵巢癌中表达上调

Journal of Cell and Molecular Research Pub Date : 2021-03-20 DOI: 10.22067/JCMR.2021.67052.0

Seyedeh Nahid Fotuhi, M. Khalaj-Kondori, Hadis Karimi

引用次数: 0

Immunological Window of Myocardial Infarction 心肌梗死的免疫窗口

Journal of Cell and Molecular Research Pub Date : 2021-03-20 DOI: 10.22067/JCMR.2021.69000.1004

Azadeh Haghighitalab, Mahboubeh Kazemi-Noughabi, S. Minaee, A. Amin, A. Bahrami

{"title":"Immunological Window of Myocardial Infarction","authors":"Azadeh Haghighitalab, Mahboubeh Kazemi-Noughabi, S. Minaee, A. Amin, A. Bahrami","doi":"10.22067/JCMR.2021.69000.1004","DOIUrl":"https://doi.org/10.22067/JCMR.2021.69000.1004","url":null,"abstract":"Acute myocardial infarction (MI) describes as an irreversible death of heart muscle which is initiated by a shortage of myocardium oxygen supply and accompanies by a complex of pro- and anti-inflammatory events. During the last decades, innate and adaptive immune responses are considered more serious for controlling myocardial infarction. As, it was confirmed that deregulated immune system which triggers excessive local and systemic inflammatory events is responsible for serious adverse effects associated with acute MI. Bone marrow activation, spleen monocytopoiesis, a remarkable increase of circulating cytokines and adhesion molecules, in addition to elevated levels of active peripheral leukocytes and platelets are playing significant roles in determining the clinical outcome of patients with MI. The previous experience demonstrated the failure of traditional harsh anti-inflammatory strategies. High mortality rate and poor quality of life observed for survivors of MI despite current progress in the field highlight the urgent need for such interdisciplinary studies in the context of molecular cardiology. Hence, unraveling the cellular and molecular events which are involved in the management of inflammatory responses post-MI is of special focus. The concept of immune regulation after myocardial infarction is not new, but our perception for dealing with the challenge has been changed during the last decades with gaining more in-depth molecular/immunological knowledge. It seems that fine-tuning the interplay between innate and adaptive immune responses and regulating their cross-talk should be in special focus to establish effective therapeutic strategies.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"12 1","pages":"74-86"},"PeriodicalIF":0.0,"publicationDate":"2021-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45042644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1