Journal of Cell and Molecular Research最新文献_第9页

Genetic analysis of ND4 and ND4L regions of mitochondrial genome in Khorasan native chickens 呼罗珊地方鸡线粒体基因组ND4和ND4L区遗传分析

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.33386

M. H. Attar, M. Nassiri

{"title":"Genetic analysis of ND4 and ND4L regions of mitochondrial genome in Khorasan native chickens","authors":"M. H. Attar, M. Nassiri","doi":"10.22067/JCMR.V6I2.33386","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.33386","url":null,"abstract":"Preserving genetic diversity of Iranian native chickens is significant in order to perform breeding programs and improved production. Genetic diversity among close populations can be determined by investigating their phylogenetic relations. Different approaches have been applied to determine the phylogenic relations from which genomic sequencing has been considered the most functional approach. In this project, we aimed to evaluate the phylogeny and genetic nucleotide sequences of ND4, ND4L regions in mitochondrial genome of Khorasan native chickens. Blood samples were collected from randomly 6 Khorasan native chicken populations and DNA were extracted. ND4L, ND4 regions were amplified using specific primers of 913 and 802 bp length, respectively. Sequencing was don according to Sanger method based on automated system. In comparison with other breeds (data extracted from NCBI), phylogeny trees and matrices regarding their genetic diversity with Khorasan native chickens for ND4, ND4L were drawn. Results showed that there is no haplotype difference between the studied samples. Lowest genetic distance was observed between Khorasan native chicken and other Asian chickens i.e. Jiangbian, Lvenwv and Red jungle fowl for the ND4, ND4L genes indicating their close relationship.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"50 1","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of IFN-γ (+874 A/T) and IL-10 (-1082 G/A) genes polymorphisms with risk of schizophrenia. IFN-γ (+874 A/T)和IL-10 (-1082 G/A)基因多态性与精神分裂症风险的分析

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.34584

D. K. Tamandani, Azizoallah Mojahed, Maryam Najafi

{"title":"Analysis of IFN-γ (+874 A/T) and IL-10 (-1082 G/A) genes polymorphisms with risk of schizophrenia.","authors":"D. K. Tamandani, Azizoallah Mojahed, Maryam Najafi","doi":"10.22067/JCMR.V6I2.34584","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.34584","url":null,"abstract":"Schizophrenia is a sophisticated mental disability which has affected nearly1.1% of people all over the world. According to recent researches, the key proteins triggered in the immune system are cytokines which might also be taking part in the pathogenesis of schizophrenia. The aim of this study was to evaluate the relationship between the -1082G/A and +874T/A polymorphisms of IL-10 and IFN-γ genes, respectively, in patients with schizophrenia. Materials and Methods: Total of 94 schizophrenic patients and 97 individuals as control samples were enrolled in this study. All samples were genotyped by ampliﬁcation mutation refractory system-polymerase chain reaction (ARMS PCR) for candidate SNPs in IFN-γ and IL-10 genes. Results: No significant association was found among various genotypes of IFN-γ and IL-10 in selected SNPs with risk of schizophrenia, As well as there was no significant variation in allelic frequency of IFN-γ and IL-10 genes with the risk of disease. Conclusion: These data suggest that the -1082G/A of IL-10 and +874T/A IFNγ genes are not involved in the development of schizophrenia risk. To validate of this data, suggesting more studies in diverse populations with larger sample size.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"64-68"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cytogenetic study and pollen viability of three populations of Diplotaxis harra (Brassicaceae) in Iran 伊朗芸苔科三居群的细胞遗传学研究及花粉活力

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.34453

M. Ranjbar, S. Karami

引用次数: 3

A comparative investigation on efficiency of bacteriophage lambda and M13 based vectors for delivering and expression of transgene in eukaryote cells 噬菌体lambda和M13载体在真核细胞中传递和表达转基因效率的比较研究

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.37598

E. Abedheydari, M. Khalaj-Kondori, Mohammad-Ali Hosseinpour-Faizi, M. Kosari-Nasab

{"title":"A comparative investigation on efficiency of bacteriophage lambda and M13 based vectors for delivering and expression of transgene in eukaryote cells","authors":"E. Abedheydari, M. Khalaj-Kondori, Mohammad-Ali Hosseinpour-Faizi, M. Kosari-Nasab","doi":"10.22067/JCMR.V6I2.37598","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.37598","url":null,"abstract":"Gene delivery might be affected by several tribulations based on carrier/vector applied. Bacteriophages lambda and M13 have different genome conformations; linear double-stranded and circular single-stranded respectively. Therefore, it might be expected that these two common classes of gene delivery vehicles will have different capacity for gene delivery and expression in eukaryote cells. To address the possible effects of linear double-stranded and circular single-stranded genome conformations of bacteriophages lambda and M13 on the transgene expression, the transfection efficacy of two vectors based on lambda and M13 were compared in AGS cell line. The GFP encoding sequence was inserted into the Lambda ZAP-CMV XR vector which resulted in λ-ZAP-CMV-GFP construct. The construct was then in vitro packaged using Gigapack® III Gold packaging extract and λ-GFP phage particles were obtained. The λ-GFP phage particles were then used for in vivo excisioning which resulted in M13-CMV-Script-GFP construct. 1011 copy of λ-ZAP-CMV-GFP or M13-CMV-Script-GFP constructs were transfected into AGS cells using lipofectamine 2000. Transfection efficiencies were analyzed by FACS. Results showed that linear double-stranded λ-ZAP-CMV-GFP was efficient than single-stranded form of M13-CMV-Script-GFP while its double-stranded form was efficient than the linear double-stranded λ-ZAP-CMV-GFP construct for transgene delivery and expression. Moreover the GFP signals resulted from transfections by single-stranded form of M13-CMV-Script-GFP construct faded more quickly in comparison to others. These findings highlight that genome conformation of gene carriers might be an important factor when seeking for an appropriate gene carrier/vehicle.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"77 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Designing a SYBR Green Absolute Real time PCR Assay for Specific Detection and Quantification of Bacillus subtilis in Dough Used for Bread Making 面包用面团中枯草芽孢杆菌的SYBR绿色绝对实时荧光定量检测方法的设计

Journal of Cell and Molecular Research Pub Date : 2014-12-13 DOI: 10.22067/JCMR.V6I2.39090

A. Sadeghi, S. Mortazavi, A. Bahrami, B. Sadeghi, M. Matin

引用次数: 7

The pluripotency feature of cancer cells; product of a harmony or an output of a disharmony 肿瘤细胞的多能性;和谐的产物或不和谐的产物

Journal of Cell and Molecular Research Pub Date : 2014-10-12 DOI: 10.22067/JCMR.V6I2.32838

Hesam Dehghani

引用次数: 0

Paternal genetic affinity between Iranian Azeris and neighboring populations 伊朗裔阿塞拜疆人和邻近人群父系遗传亲缘关系

Journal of Cell and Molecular Research Pub Date : 2014-06-29 DOI: 10.22067/JCMR.V6I1.31978

A. Bahmanimehr, F. Nikmanesh

{"title":"Paternal genetic affinity between Iranian Azeris and neighboring populations","authors":"A. Bahmanimehr, F. Nikmanesh","doi":"10.22067/JCMR.V6I1.31978","DOIUrl":"https://doi.org/10.22067/JCMR.V6I1.31978","url":null,"abstract":"In certain environments such as Iran highlands, major innovations in lifestyle, as the emergence of agriculture and domestication of animals, are thought to have led to population expansions. Historical studies showed that at some point in history (from the third to the first millennium BC) dramatic changes have been taken place on the Iranian plateau. To trace the genetic affinity between the Iranian Azeris and neighboring populations, 297 samples were collected from northwest of Iran. The non-recombining portion of the Y chromosome (NRY) was genotyped at the unique event polymorphism (UEP) levels, using 48 single-nucleotide polymorphism (SNP) markers, based on the human NRY tree. According to our results, like other Iranian ethnic groups, Iranian Azeris showed a heterogeneous paternal genetic structure. Low genetic distances were also found between Iranian Azeris and their contemporary geographical neighbors. They also have preserved minor share of Y-haplogroup of central Asian ancestry tracts in their genomes, which is in agreement with the historical period of major Turkic migrations. The strategic feature for northwest of Iran to transfer and carry important ancient migratory events and gene flow across the Asia and the Europe also conducive conditions for sedentary habitation leading to sharp demographic growth in the area is supported by all molecular and statistical analysis of this study.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"73 1","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"2014-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations on chromosome variation in Achillea tenuifolia Lam and A. bieberestinii Afan (Asteraceae) from the West of Iran 伊朗西部地区菖蒲和菊科菖蒲染色体变异的研究

Journal of Cell and Molecular Research Pub Date : 2014-06-29 DOI: 10.22067/JCMR.V6I1.26290

A. C. Rad, F. Mohsenzadeh, H. Salehi

引用次数: 0

ATP-binding cassette (ABC) transporters as emerging targets in modulation of neural stem cells behavior in neurodegenerative diseases and cell therapy benefits atp结合盒(ABC)转运体作为神经退行性疾病中神经干细胞行为调节的新靶点和细胞治疗益处

Journal of Cell and Molecular Research Pub Date : 2014-06-29 DOI: 10.22067/JCMR.V6I1.30375

S. Vishwakarma, S. Paspala, S. Tiwari, A. Khan

{"title":"ATP-binding cassette (ABC) transporters as emerging targets in modulation of neural stem cells behavior in neurodegenerative diseases and cell therapy benefits","authors":"S. Vishwakarma, S. Paspala, S. Tiwari, A. Khan","doi":"10.22067/JCMR.V6I1.30375","DOIUrl":"https://doi.org/10.22067/JCMR.V6I1.30375","url":null,"abstract":"Increasing incidence of central nervous system (CNS) disorders has become a major challenge for both basic and clinical scientific society to develop novel therapeutic models for treatment. The knowledge of stem cells has added a new dimension in the research towards finding more appropriate targets responsible for the disease fate determination. As stem cell research is progressing day by day in routine research laboratories there is great hope to find suitable therapeutic targets for complete cure of the CNS disorders. Discovery of ABC transporters in animal tissues has emerged as new spot for several disease prognosis and therapeutic target. ABC transporters are membrane proteins expressed in various organs like liver, kidney, blood-brain barrier, blood-testis barrier etc. It is involved in various important cellular processes such as absorption, distribution and excretion of drugs, xenobiotics and endogenous compounds showing their role in tissue defense and organ regeneration. The current review explains about the role of ABC transporters in CNS pathogenesis and defense adding stem cells therapeutic strategies.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"44-49"},"PeriodicalIF":0.0,"publicationDate":"2014-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

PCR-SSCP: a method to fingerprinting sequence variability in plant gene pool using the Iranian wild diploid Triticum gene pool as a case study PCR-SSCP:一种植物基因库序列变异指纹图谱方法，以伊朗野生二倍体小麦基因库为例

Journal of Cell and Molecular Research Pub Date : 2014-06-29 DOI: 10.22067/JCMR.V6I1.31099

F. Nasernakhaei, M. Rahiminejad, H. Saeidi, M. Tavassoli

引用次数: 1