Journal of Cell and Molecular Research最新文献_第8页

Cloning and Expression of Fusion (F) and Haemagglutinin-neuraminidase (HN) Epitopes in Hairy Roots of Tobacco (Nicotiana tabaccum) as a Step Toward Developing a Candidate Recombinant Vaccine Against Newcastle Disease 烟草毛状根融合(F)和血凝素-神经氨酸酶(HN)抗原表位的克隆与表达及其对新城疫候选重组疫苗的研究

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/JCMR.V7I1.41621

A. Shahriari, A. Bagheri, M. Bassami, S. M. Shafaroudi, A. Afsharifar

{"title":"Cloning and Expression of Fusion (F) and Haemagglutinin-neuraminidase (HN) Epitopes in Hairy Roots of Tobacco (Nicotiana tabaccum) as a Step Toward Developing a Candidate Recombinant Vaccine Against Newcastle Disease","authors":"A. Shahriari, A. Bagheri, M. Bassami, S. M. Shafaroudi, A. Afsharifar","doi":"10.22067/JCMR.V7I1.41621","DOIUrl":"https://doi.org/10.22067/JCMR.V7I1.41621","url":null,"abstract":"Newcastle is a significant avian disease continuing to cause considerable loss. Developments in genetic engineering have led to plant-based platforms for human and animal vaccine production. Recombinant vaccine production in hairy root systems have several advantages over stable expression in whole plants, including high growth rates, ready genetic manipulations, high levels of recombinant protein production, and the potential for bioreactor culture. In an attempt to develop a recombinant vaccine in hairy roots, the sequences encoding fusion (F) and haemagglutinin-neuraminidase (HN) epitopes of Newcastle disease virus were cloned in pBI121 expression vector which was then transferred into leaf disks of tobacco (Nicotiana tabaccum) 'Turkish' cultivar by means of Agrobacterium rhizogenes. Hairy roots developed on MS medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Incorporation of the heterologous gene in the genome of hairy roots was confirmed by PCR. Expression analyses were performed by real-time PCR at transcription level and by dot-blot and ELISA assays at translation level, all confirming the expression of the heterologous gene and production of the recombinant protein.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"11-18"},"PeriodicalIF":0.0,"publicationDate":"2015-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Identification of PI3K Isoforms in Human Prostate Cancer Cell Lines (PC3, DU145) and Human Bladder Carcinoma Cell line (5637) 人前列腺癌细胞PC3、DU145和膀胱癌细胞5637中PI3K亚型的鉴定

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/jcmr.v7i1.40028

H. Aryan, Z. Soheili

{"title":"Identification of PI3K Isoforms in Human Prostate Cancer Cell Lines (PC3, DU145) and Human Bladder Carcinoma Cell line (5637)","authors":"H. Aryan, Z. Soheili","doi":"10.22067/jcmr.v7i1.40028","DOIUrl":"https://doi.org/10.22067/jcmr.v7i1.40028","url":null,"abstract":"There exists an association between PI3K pathway licentious activity and the considerable feature of high metastatic potential of the genitourinary cancer cells. Although DU 145 and 5637 have functional phosphatase and tensin homolog (PTEN) tumor suppressor gene, which antagonizes PI3K function, PC-3 is null for PTEN gene. In pursuit to explain why PTEN bearing cell lines display high metastatic behavior, we searched for any discrepancy in PI3K isoforms expression pattern between these cell lines. Gathering gene bank data files, specific primers were designed, for all the genes of 12 studied isoforms from 3 different classes of PI3K. Total RNA was extracted and examined by Real- Time PCR to compare the cells for the type and amount of the isoforms which expressed. Cα and R2 isoforms are indicative of an equal expression for PC3 and DU145, R3 transcripts revealed 80% decrease in DU145 and Cβ, R1 and C2α demonstrated an increased expression in DU145. When a comparison is made between 5637 and PC3, it can be seen that although a little decrease in the level of R3 transcripts was demonstrated, the amount of Cα, Cβ, R2, R1 and C2α increased. In conclusion in this study it is proposed that R1, R2, Cα, Cβ , C2α and R1, Cβ , C2α are candidate genes for silencing via RNAi in 5637 and DU145, respectively, to evaluate their roles in metastatic behavior of the both studied PTEN bearing cell lines.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"03-10"},"PeriodicalIF":0.0,"publicationDate":"2015-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Acipenser Persicus Growth Hormone gene Sequencing and its structures 猕猴生长激素基因序列测定及其结构研究

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/JCMR.V7I1.41102

Ehsan Nasr, M. Pourkazemi, H. Hovhannisyan

引用次数: 1

Evaluation of Agrobacterium-mediated Transformation of Chlamydomonas reinhardtii Using a Synthetic Amorpha-4, 11-diene Synthase Gene 农杆菌介导的合成amorpha4,11 -二烯合酶基因转化莱茵衣藻的研究

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/JCMR.V7I1.36923

A. Mohkami, H. Marashi, Farajolah Shahriary Ahmadi, M. Tohidfar, M. Mohsenpour

引用次数: 2

Renin-Angiotensin A1166C Polymorphism and the Rrisk of Stroke 肾素-血管紧张素A1166C多态性与卒中风险的关系

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/JCMR.V7I1.38359

Peyman Zargari, M. Ghasemi, M. Pirhoushiaran, V. Vakili, J. Hami, M. Farzadfard, Payam Sasan-Nezhad, M. Azarpazhooh, A. Sadr-Nabavi

{"title":"Renin-Angiotensin A1166C Polymorphism and the Rrisk of Stroke","authors":"Peyman Zargari, M. Ghasemi, M. Pirhoushiaran, V. Vakili, J. Hami, M. Farzadfard, Payam Sasan-Nezhad, M. Azarpazhooh, A. Sadr-Nabavi","doi":"10.22067/JCMR.V7I1.38359","DOIUrl":"https://doi.org/10.22067/JCMR.V7I1.38359","url":null,"abstract":"Stroke is the leading cause of death and disability in the world after the cancer and cardiovascular diseases. Genetic factors have main significance to got stroke. Renin-angiotensin system contains candidate genes and polymorphisms for causing stroke. There are reported associations between stroke and angiotensin II type-1 receptor g. 1166A > C polymorphism (rs5186). Therefore in this study this association was investigated for the east Iranian population. This study is based on 201 stroke patients and 220 controls. To predict the genetic risk of stroke allele and genotype frequencies of angiotensin II type-1 receptor rs5186 were analyzed in this population according to stroke subtypes, gender, age, hypertension, diabetes mellitus, high and low density lipoprotein and triglycerides. According to statistical analysis no significant difference was found between case and control groups. But there were a significant relevance between total cholesterol and stroke (p = 0.037). In this population angiotensin II type-1 receptor g. 1166A > C polymorphism did not increase the risk of stroke. The main reason for this study is complex nature of gene-environment interactions in the pathophysiology of this disease.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2015-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association of miR-132 and miR-185 Genes Methylation and their Expression Profile with Risk of Congenital Factor XIII Deficiency miR-132和miR-185基因甲基化及其表达谱与先天性因子XIII缺乏风险的关联

Journal of Cell and Molecular Research Pub Date : 2015-06-25 DOI: 10.22067/JCMR.V7I1.38961

D. Kordi-Tamandani, Z. Rezaei, Akbar Dorgalaleh-Mail

{"title":"Association of miR-132 and miR-185 Genes Methylation and their Expression Profile with Risk of Congenital Factor XIII Deficiency","authors":"D. Kordi-Tamandani, Z. Rezaei, Akbar Dorgalaleh-Mail","doi":"10.22067/JCMR.V7I1.38961","DOIUrl":"https://doi.org/10.22067/JCMR.V7I1.38961","url":null,"abstract":"Congenital factor XIII deficiency is a very rare bleeding disorder, but because of the high rate of consanguineous marriages, it is common in Sistan and Baluchestan Province of Iran. The discovery of promoter hypermethylation of numerous miRNAs in human diseases has demonstrated an epigenetic mechanism for aberrant miRNA expression. The present study has analyzed methylation and expression status of miR-185 and miR-132 genes in patients with inherited factor XIII deficiency in a sample of South-Eastern Iranian population. Promoter methylation of miR-185 and miR-132 was investigated by Methylation Specific Polymerase Chain Reaction (MS PCR) in blood samples of 75 factor XIII deficient individuals and 74 healthy controls. Expression level of these genes was also assessed in 15 blood samples of patients and 15 healthy controls using real-time quantitative reverse transcription PCR. Analysis of miR-132 and miR-185 promoter hypermethylation did not show any significant difference between cases and controls. Relative gene expression analysis in cases (n=15) with congenital factor XIII deficiency and healthy controls (n=15) revealed no statistically significant relationship for miR-132 (p = 0.126) and miR-185 (p = 0.165) genes. Our findings indicated that promoter methylation as well as gene expression of miR-132 and miR-185 had no significant effect on etiology of factor XIII deficiency.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"7 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"2015-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant vaccine production in green plants: State of art 绿色植物中的重组疫苗生产:最新进展

Journal of Cell and Molecular Research Pub Date : 2015-04-18 DOI: 10.22067/JCMR.V7I1.45074

M. Habibi-Pirkoohi, A. Mohkami

引用次数: 9

Impact of MTHFR and RFC-1 gene in the development of neural tube defect MTHFR和RFC-1基因在神经管缺损发生中的影响

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/jcmr.v6i2.35894

R. Kumari, A. Agrawal, O. P. Upadhyaya, G. P. Singh, G. Dubey

{"title":"Impact of MTHFR and RFC-1 gene in the development of neural tube defect","authors":"R. Kumari, A. Agrawal, O. P. Upadhyaya, G. P. Singh, G. Dubey","doi":"10.22067/jcmr.v6i2.35894","DOIUrl":"https://doi.org/10.22067/jcmr.v6i2.35894","url":null,"abstract":"Neural tube defects (NTDs) are complex problem of central nervous system including brain and spinal cord. Anencephaly and myelomeningocele are the two most common forms of NTDs. Epidemiological studies reveal that genetic and environmental factors are responsible for the development of NTDs. During embryogenesis large numbers of extrinsic and intrinsic factors are responsible for the closer of neural tube which is responsible to maintain the three germ layers including neural ectoderm. The role of MTHFR & RFC-1 gene in etiopathology of NTDs has not been clearly defined in Indian population. Hence, the curiosity has been developed with the aim to evaluate folate metabolism and folate regulatory gene in clinically diagnosed NTDs by using PCR based DNA analysis with selected specific forward/reverse primers. Interestingly, the highest frequency (12.5%) of CT has been appeared of MTHFR C677T gene noticed in NTDs mother. We also observed the similar frequency of heterozygous AG genotype in NTDs of A80G RFC-1 gene. Therefore, C and A allele have high prevalence among than other genotype. However, the mutation in MTHFR partially have protective effect of embryo and these selected candidate folate markers are responsible to influence the cells of neural crest confirming the folding of neural tube associated with severity of disease in NTDs.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"103-104"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuronal differentiation of mouse amnion membrane derived stem cells in response to neonatal brain conditioned medium. 小鼠羊膜来源干细胞对新生儿脑条件培养基的神经元分化反应。

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.37591

ُSheida Shahraki, H. Jalali, K. Parivar, N. Roudbari, M. Nabiuni, Z. Heidari

{"title":"Neuronal differentiation of mouse amnion membrane derived stem cells in response to neonatal brain conditioned medium.","authors":"ُSheida Shahraki, H. Jalali, K. Parivar, N. Roudbari, M. Nabiuni, Z. Heidari","doi":"10.22067/JCMR.V6I2.37591","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.37591","url":null,"abstract":"Background: Amniotic membrane derived stem cells (AMSCs) have considerable advantages to use in regenerative medicine and their anti- inflammatory effects, growth factor secretion and differentiation potential make them suitable candidates for stem cell therapy of nervous system. The developing and neonatal brain contains a spectrum of growth factors to direct development of endogenous and donor cells. Using an in vitro model system, we investigated the plasticity and potential of mouse AMSCs to differentiate into neural cells in response to neonatal mice brain extracted medium. \u0000Methods: Mouse amniotic membrane stem cells were isolated from embryos, cultured in presence of medium derived from neonatal mouse brain medium and immunohistochemistry and flow cytometry analyses were used to explore the neural differentiation of them. \u0000Results: Isolated amnion membrane stem cells showed high rate of viability and proliferation and presented neural characters in the presence of neonatal brain extracted medium such morphological changes and Nestin and Map-2 expression. \u0000Conclusion: In conclusion, results from this study showed that amnion membrane derives stem cells are potent stem cells to respond to environmental signals promoting them to neural fate.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"76-82"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68592331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing microRNA quantification in serum samples 优化血清样品中microRNA的定量

Journal of Cell and Molecular Research Pub Date : 2014-12-25 DOI: 10.22067/JCMR.V6I2.31710

S. Gharbi, F. Mirzadeh, Shahriar Khatrei, M. Soroush, M. Tavallaie, M. Nourani, mehdi Sahmsara, S. J. Mowla

{"title":"Optimizing microRNA quantification in serum samples","authors":"S. Gharbi, F. Mirzadeh, Shahriar Khatrei, M. Soroush, M. Tavallaie, M. Nourani, mehdi Sahmsara, S. J. Mowla","doi":"10.22067/JCMR.V6I2.31710","DOIUrl":"https://doi.org/10.22067/JCMR.V6I2.31710","url":null,"abstract":"MicroRNAs constitute a group of small non-coding RNAs that negatively regulate gene expression. Aside from their contribution to biological and pathological pathways, altered expression of microRNAs is reported in bio-fluid samples, such as serum. To employ serum's microRNAs as potential biomarkers, it is crucial to develop an efficient method for microRNA quantification, avoiding pre-analytical and analytical variations which could affect the accuracy of data analysis. Here, we optimized a real-time PCR quantification procedure for microRNA detection in serum samples. Serum's total RNA was extracted using two different RNA isolation methods, one based on phenol-chloroform and the other based on silica column. To investigate a potential PCR inhibitory effect, different RNA amounts were subjected to reverse transcription. Moreover to assess the enzymatic efficiency, synthetic exogenous microRNAs was spiked into the mixture. Moreover, to find a reliable internal control gene for normalizing the microRNA quantification, the amounts of 8 candidate non-coding RNAs including SNORD38B, SNORD49A, U6, 5S rRNA, miR-423-5p, miR-191, miR-16 and miR-103 were assessed on serum samples. Altogether, our data demonstrated that the silica-based method was more efficient for microRNA recovery. Furthermore, increasing the input volume of the extracted RNA would dramatically increase inhibitors' amounts which could end up in a larger Cq values. Therefore, the best input volume of RNA turned out to be 1.5 microliter/reaction. Among the 8 aforementioned internal controls, U6, SNORD38B and SNORD49A showed low levels of expression, and were undetectable in some samples. Amongst the others, 5s rRNA, had the biggest standard deviation which could significantly affect data analysis. MiR-103 with the least variation appeared to be the best normalizer gene.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"6 1","pages":"52-56"},"PeriodicalIF":0.0,"publicationDate":"2014-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68591430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3