Journal of Cell and Molecular Research最新文献_第5页

Biological Activity of Persian Sturgeon Recombinant Growth Hormone Molecules Trapped In Inclusion Bodies (IBs) 包涵体中重组生长激素分子的生物活性研究

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/JCMR.V9I1.53803

Ehsan Nasr, H. Hovhannisyan, P. Mohammad

引用次数: 0

Investigation of Genetic Variation In Berberis Vulgaris Using ISSR and SSR Molecular Markers ISSR和SSR分子标记研究小檗遗传变异

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/JCMR.V9I1.62712

Behnaz Safamanesh, S. E. Bahabadi, A. Izanloo

{"title":"Investigation of Genetic Variation In Berberis Vulgaris Using ISSR and SSR Molecular Markers","authors":"Behnaz Safamanesh, S. E. Bahabadi, A. Izanloo","doi":"10.22067/JCMR.V9I1.62712","DOIUrl":"https://doi.org/10.22067/JCMR.V9I1.62712","url":null,"abstract":"Barberry fruit is a medicinal plant, and it is one of the most important horticultural crops in South Khorasan province, Iran. Genetic diversity has a basic role in the successful breeding of crop varieties with durable resistance to biotic and abiotic stresses. The main objective of this study was to assess the genetic diversity of 20 ecotypes of Berberis, which collected from different regions of South Khorasan province, Iran, using ISSR and SSR markers. In this study, 10 ISSR primers and 5 SSR primer pairs, with the amplification of suitable polymorphic alleles were used. A total of 98 bands for ISSR markers and 43 bands for SSR markers were detected between 300 to 1300 bp and 100 to 1100 bp in size, respectively. Polymorphic ISSR-7 and CA03 primers amplified the highest number of alleles with 17 and 24 bands, respectively, while ISCS50 and CA30 primers amplified only two polymorphic alleles. The ISCS57 and GA31 primers had the highest polymorphic information content (PIC) and ISCS50 and GA04 primers had the lowest PIC. The estimated Nei's and Shannon indices for genetic diversity in ISSR markers were 0.24 and 0.35, while for SSR markers these were 0.23 and 0.34, respectively. Based on cluster analysis, five and six main groups were identified for ISSR and SSR markers, showing high genetic variations among a set of collected barberry ecotypes. Analysis of molecular variances in both ISSR and SSR markers showed that high level of total variation was due to within populations, rather. Therefore, it will be better to select within populations in breeding programs.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"23-34"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47211041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparison of Human Factor IX Abundance and mRNA Expression Levels In Stable Insect and Mammalian Cells 人因子IX在稳定昆虫和哺乳动物细胞中丰度和mRNA表达水平的比较

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/JCMR.V9I1.61046

J. Vatandoost, Shadi Tirdad

{"title":"Comparison of Human Factor IX Abundance and mRNA Expression Levels In Stable Insect and Mammalian Cells","authors":"J. Vatandoost, Shadi Tirdad","doi":"10.22067/JCMR.V9I1.61046","DOIUrl":"https://doi.org/10.22067/JCMR.V9I1.61046","url":null,"abstract":"In the production of recombinant proteins, the selection of an expression system is very important. Although CHO cells are used as mammalian expression system, the ability of insect Schneider line 2 (S2) cells as a new expression system for the high production of many human proteins were confirmed. It is suggested that high copy number of an introduced gene and so high transcription in these cells can be regarded as one of the reasons for high expression of recombinant proteins. Therefore, the present study aimed to evaluate the correlation of recombinant human coagulation factor IX (hFIX) abundance and mRNA expression. The amount of hFIX mRNA by quantitative real-time PCR as well as hFIX protein in cell lysate and cultured media by Elisa was analyzed. The results of data analysis indicated 6 fold increases in mRNA level in S2 cells in comparison to CHO cells. Furthermore, S2 cell line indicated 5.5 and 7-fold increase in total and secreted protein level, respectively, compared to CHO cell line. The data demonstrated the correlation of mRNA and protein abundance and indicate that S2 cell lines are superior in producing the recombinant proteins.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45648087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Chlamydophila Abortus In Aborted Fetal Tissues by Using Polymerase Chain Reaction (PCR) In Tabriz, Northwest of Iran 应用聚合酶链式反应（PCR）对伊朗西北部大不里士流产胎儿组织中流产衣原体的分子检测

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/JCMR.V9I1.59810

Mahsa Alem, R. Asadpour, R. Joozani, K. Nofouzi

引用次数: 3

Construction of the Recombinant Lentiviral Vector Containing Human GH1 Gene and Its Expression in HEK293T Cells 人GH1基因重组慢病毒载体的构建及其在HEK293T细胞中的表达

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/jcmr.v8i2.22857

Z. Roudbari, M. Nassiri, M. Tahmoorespur, A. Haddad-Mashadrizeh, A. Javadmanesh

{"title":"Construction of the Recombinant Lentiviral Vector Containing Human GH1 Gene and Its Expression in HEK293T Cells","authors":"Z. Roudbari, M. Nassiri, M. Tahmoorespur, A. Haddad-Mashadrizeh, A. Javadmanesh","doi":"10.22067/jcmr.v8i2.22857","DOIUrl":"https://doi.org/10.22067/jcmr.v8i2.22857","url":null,"abstract":"Human growth hormone (hGH) is a protein with multiple roles in a range of biological functions such as protein, carbohydrates and lipid metabolisms as well as immunity, tissue development and overall growth. One of the major class of biopharmaceuticals in mammalian cells is the production of recombinant pharmaceutical proteins. In this study, we constructed a lentiviral vector carrying coding region of human GH1 (hGH) gene in order to production of recombinant hGH in mammalian cell line. hGH gene was amplified from a plasmid containing full-length hGH coding sequence and then cloned into the lentiviral vector pCDH-GFP. The HEK293T cells were transduced by the lentivirus particles as a targeted cell. hGH expression status in the recombinant cells were confirmed by RT-PCR. Additionally, western blotting analysis results showed that the recombinant cells maintained a stable hGH expression during five weeks of continuous culture. In conclusion, results of current study suggested that constructed lentiviral vector can potentially be used for a stable production of recombinant hGH protein in HEK293T cells. This methodology could be served as a foundation for further research and may open new insights toward therapeutic protein manufacturing.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2016-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Characterization of an Extracellular Phosphatase Enzyme From Bacillus spp. 芽孢杆菌胞外磷酸酶的纯化及特性研究。

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/JCMR.V8I2.58676

Maryam Parhamfar, Arastoo Badoei-dalfard, Milad Parhamfar, Shohreh Fahimi Rad

{"title":"Purification and Characterization of an Extracellular Phosphatase Enzyme From Bacillus spp.","authors":"Maryam Parhamfar, Arastoo Badoei-dalfard, Milad Parhamfar, Shohreh Fahimi Rad","doi":"10.22067/JCMR.V8I2.58676","DOIUrl":"https://doi.org/10.22067/JCMR.V8I2.58676","url":null,"abstract":"Phosphorus is one of the most important nutrients for plant growth and development. Chemical Pi fertilizer is used to provide the phosphorus for the plants, but it is mostly fixed in the soil into insoluble form and become unavailable to the plants. Phosphate-solubilizing bacteria have lots of application in agriculture as biological fertilizer. Consumption of biofertilizers instead of chemical fertilizers can lead to environmental pollution reduction and crop production enhancement using sustainable farming. In this study, a phosphatase-producing bacterium was isolated from agricultural soil in Kerman. Screening of phosphate solubilizing bacteria was performed on the PVK medium, based on clear area diameter. The best bacterium (AG41) was identified based on 16s rDNA gene. The optimum condition for production of phosphatase was also determined and it was purified and characterized. Sequence alignment and phylogenetic tree results show that AG41 is closely related to Bacillus subtilis, with 98% homology. Phosphatase activity was determined by end point method. The best carbon, nitrogen and phosphate sources for enzyme production were 1.0% glucose, 0.5% ammonium sulfate and (0.25%) sodium phytate +(0.25%) tricalcium phosphate, respectively. Bacterial phosphatase was partially purified using ammonium sulfate fractionation followed by dialysis. Results showed that the optimum temperature for the purified enzyme activity was 40oC and it was stable at temperatures below 60°C. This enzyme was stable between pH 3.0-7.0, and the optimal pH activity was found to 5.0. These results indicated that this strain can be a notable candidate for using as biofertilizers.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"113 1","pages":"90-97"},"PeriodicalIF":0.0,"publicationDate":"2016-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Association of Codon 72 of P53 Gene Polymorphism with Chronic Hepatitis C Virus Infection: A Case Control Study P53基因密码子72多态性与慢性丙型肝炎病毒感染的关系:一项病例对照研究

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/jcmr.v8i2.56120

Sina Gerayli, Alireza Pasdar, S. Rostami, S. Sepahi, S. M. Hoseini, R. Jahanian, Aida Gholoobi, Z. Meshkat, M. Ahadi

{"title":"Association of Codon 72 of P53 Gene Polymorphism with Chronic Hepatitis C Virus Infection: A Case Control Study","authors":"Sina Gerayli, Alireza Pasdar, S. Rostami, S. Sepahi, S. M. Hoseini, R. Jahanian, Aida Gholoobi, Z. Meshkat, M. Ahadi","doi":"10.22067/jcmr.v8i2.56120","DOIUrl":"https://doi.org/10.22067/jcmr.v8i2.56120","url":null,"abstract":"Single nucleotide polymorphism in codon 72 of p53 gene (Arg/Pro) changes p53 protein structure and affects its activities. Hepatitis C virus (HCV) is believed to induce hepatocellular carcinoma and P53 polymorphisms have been associated with human cancers. The aim of this study was to evaluate genetic variants of codon 72 of p53 gene polymorphism in HCV patients and its relationship with HCV infection. \u0000The study was conducted on 67 HCV patients, who were referred to medical centers of Mashhad city, Iran, and 73 healthy people from the same region. Genotyping of codon 72 of p53 gene was performed by PCR-RFLP method. \u0000The distributions of different alleles of p53 polymorphisms did not differ significantly between groups. The respective proportions of Proline homozygotes, heterozygotes, and Arginine homozygotes were 37.31%, 35.82%, 26.86% in patients and 39.72%, 27.39%, and 32.87% in the control group respectively. However, we found no significant differenece for the allelic or genotype distribution between cases and controls. \u0000Our results indicated no strong evidence of association of the p53 polymorphism with HCV infection; however, further investigation is needed in different ethnic groups to elucidate the role of this polymorphism in HCV infection.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"46-51"},"PeriodicalIF":0.0,"publicationDate":"2016-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68593829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration 苜蓿口蹄疫病毒(FMDV)外壳蛋白在农业渗透中的瞬时表达

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/jcmr.v8i2.44884

M. Habibi-Pirkoohi, S. Malekzadeh-Shafaroudi, H. Marashi, S. Zibaee, A. Mohkami, Saba NejatizaXeh

引用次数: 2

Protective Effect of Diosgenin against H2O2-Induced Oxidative Stress on H9C2 Cells 薯蓣皂苷元对h2o2诱导的H9C2细胞氧化应激的保护作用

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/JCMR.V0I0.36148

Samaneh Jamshidi, M. Lahouti, M. Boroushaki, A. Ganjeali, A. Ghorbani, Mehdi Bihamta Toosi

{"title":"Protective Effect of Diosgenin against H2O2-Induced Oxidative Stress on H9C2 Cells","authors":"Samaneh Jamshidi, M. Lahouti, M. Boroushaki, A. Ganjeali, A. Ghorbani, Mehdi Bihamta Toosi","doi":"10.22067/JCMR.V0I0.36148","DOIUrl":"https://doi.org/10.22067/JCMR.V0I0.36148","url":null,"abstract":"Diosgenin is an important compound in pharmaceutical industry. It has various effects such as hypocholesterolemic action or antioxidant activity in HIV infected patients. Biological oxidation pathways are involved in causing or aggravating heart disease. This study investigated the potential protective effect of diosgenin on cell viability and antioxidant defenses of cultured H9C2 cells submitted to oxidative stress induced by H2O2. Viability of cells exposed to H2O2 was detected by MTT assay. The generation of ROS and hydrogen peroxide release after H2O2 were detected using the fluorescent probe H2DCF-DA. The lipid peroxidation product i.e. MDA formation was estimated by assessing the levels of thio-barbituric acid reactive substances (TBARS) using spectrophotometry. SOD activity was assayed with NWLSS (TM) Superoxide Dismutase (SOD) activity assay kit. Pretreatment of cells with 3-25 µM of diosgenin for 24 h before applying H2O2 completely prevented cell damage and significantly enhanced viability of H9C2 cells. Increased ROS induced by H2O2 was dose dependently prevented when cells were pretreated for 24 h with diosgenin. The level of the lipid peroxidation was significantly higher in H9C2 cells exposed to H2O2 as compared to the control and cells pretreated with diosgenin. SOD activity in cells treated with diosgenin significantly decreased compared with cells exposed to H2O2. These results show that treatment of H9C2 cells with diosgenin (3-25 µM) confers a significant protection against oxidative stress.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"8 1","pages":"58-64"},"PeriodicalIF":0.0,"publicationDate":"2016-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68587545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Unravelling Over-Represented Amino Acids in Protein Structure of Allergen Proteins; A Large-Scale Study 揭示过敏原蛋白结构中代表性过强的氨基酸大规模研究

Journal of Cell and Molecular Research Pub Date : 2016-12-25 DOI: 10.22067/jcmr.v0i0.60190

N. Rahmani, E. Ebrahimie, A. Niazi, Najaf Allahyari Fard, B. Bambai, Z. Minuchehr, M. Ebrahimi

引用次数: 1