Journal of Cell and Molecular Research最新文献_第4页

MicroRNA-mediated Resistance to Plant Viruses microrna介导的植物病毒抗性

Journal of Cell and Molecular Research Pub Date : 2018-09-30 DOI: 10.22067/JCMR.V10I1.70766

A. Shahriari, امین الله طهماسبی

引用次数: 0

The Effects of Silveroxide on mRNA Level of Key Genes Involved In the Biosynthesis of Rebaudioside-A in Stevia Rebaudiana 氧化银对甜菊合成瑞巴糖苷-A关键基因mRNA水平的影响

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/JCMR.V9I2.69816

A. Mirzaei, S. Dezhsetan, Gholamreza Kavoosi, M. Behnamian

{"title":"The Effects of Silveroxide on mRNA Level of Key Genes Involved In the Biosynthesis of Rebaudioside-A in Stevia Rebaudiana","authors":"A. Mirzaei, S. Dezhsetan, Gholamreza Kavoosi, M. Behnamian","doi":"10.22067/JCMR.V9I2.69816","DOIUrl":"https://doi.org/10.22067/JCMR.V9I2.69816","url":null,"abstract":"Stevia rebaudiana bertoni is a plant with sweetening properties. This medicinal plant is perennial and from Asteraceae family. Its leaves contain glycoside compounds of a sugar part and non-sugar sectors. One of the glycosides compounds is Rebaudioside¬A which has a greater importance in market. Several key regulating genes including copalyl diphosphate synthase (CPPS) (AF034545.1), geranylgeranyl diphosphate synthase (GGDPS) (DQ432013.3), (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) (FJ755689.1), UDP glucosyltranserase 85C2(UGT85C2)(AY345978.1), UDPglucosyltranserase–74G1 (UGT74G1) (AY345982.1) and UDP glucosyltranserase–76G1 (UGT76G1) (KC631816.1) are involved in the biosynthesis Rebaudioside A. This experiment was conducted to evaluate the effect of silver oxide Ag2O on the mRNA level of these genes in the stevia rebaudiana. The experiments repeated 3 times and with concentrations of 50, 100 and 200 µM. Increasing concentrations of 50 micromoles of silver oxide up to 100 micromoles leads to an increase in the expression levels of all the studied genes. Also according to the expression profile of these genes and the results of HPLC there is a significant increase on the expression level of the genes and production of Rebaudioside¬A under Ag2O treatment. In general, it was found that increasing the concentration of Ag2O can lead to an increase in the level of mRNA for the chosen genes. On the other hand, the low expression of the genes studied under control conditions (No Treatment), compared to the treatment with Ag2O, revealed that the treatment can lead to higher sweetener glycoside components in the Stevia leaves. The physiological assay showed that Ag2O treatment in concentrations of 100 and 200 µM have more positive effect on chlorophyll, protein, carbohydrates and carotenoids in Stevia.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49495606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyosystematics of an Endemic tooth-carp, Aphanius shirini (Teleostei: cyprinodontidae) from Iran 伊朗一特有齿鱼的核系统学研究

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/JCMR.V9I2.67315

A. Mansoori, M. Ebrahimi, A. Gholamhosseini, H. Esmaeili

引用次数: 0

Karyological Data of Tanacetum polycephalum Schultz-bip. and T. parthenium Schultz-bip. (Asteraceae) Populations 多头Tanacetum polycephalum Schultz-bip的核学资料。和T. parthenium Schultz-bip。(菊科)人口

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/JCMR.V9I2.59568

H. Javadi

{"title":"Karyological Data of Tanacetum polycephalum Schultz-bip. and T. parthenium Schultz-bip. (Asteraceae) Populations","authors":"H. Javadi","doi":"10.22067/JCMR.V9I2.59568","DOIUrl":"https://doi.org/10.22067/JCMR.V9I2.59568","url":null,"abstract":"Chromosome numbers in 19 populations of Tanacetum polycephalum and Tanacetum parthenium from natural resources gene bank, that collected from different regions of Iran, were determined for the first time. The samples prepared by using root tips. After pretreatment, fixation, hydrolysis and staining, the microscopic samples prepared by squash method, metaphases were captured using an optical microscope. The best metaphases plates were selected and used for karyotype analyses. In all of populations the basic chromosome number was x=9 and the populations showed two ploidy levels (diploid & tetraploid). The type of the most chromosomes in all of the populations was metacentric (m) and sub-metacentric (sm) and located in 2A and 2B except for T. parthenium (Yazd, Taft) with 5m+3sm+1st karyotype formula and 2C Stebbins classes. In addition, T. polycephalum (Esfahan, Golpayegan) with the highest value of AR and A1 had karyotype heterogeneity, also T. polycephalum (Esfahan, Golpayegan) and T. polycephalum (West-Azerbaijan, Uromeyeh) had the highest value of chromosome length (TL). Detailed karyotype allows us to group the different populations based on Stebbins classes and asymmetry indices.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"59-66"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43752623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA Binding Site Polymorphisms are Associated with the Development of Gastric Cancer 微小核糖核酸结合位点多态性与癌症的发生发展

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/jcmr.v9i2.71487

Jamal Asgarpour, J. Mehrzad, S. A. T. Yazdi

引用次数: 1

Identification of Non-Ribosomal Peptide Synthetase Modifications Involved in Surfactin Production and Quorum-sensing Operon of Bacillus subtilis MJ01 Isolated from Oil-Contaminated Soil 从石油污染土壤中分离的枯草芽孢杆菌MJ01表面活性素产生和群体感应操作子的非核糖体肽合成酶修饰的鉴定

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/jcmr.v9i2.64586

T. Rahimi, A. Niazi, S. Taghavi, E. Ebrahimie, S. Ayatollahi, Tahereh Deihimi

{"title":"Identification of Non-Ribosomal Peptide Synthetase Modifications Involved in Surfactin Production and Quorum-sensing Operon of Bacillus subtilis MJ01 Isolated from Oil-Contaminated Soil","authors":"T. Rahimi, A. Niazi, S. Taghavi, E. Ebrahimie, S. Ayatollahi, Tahereh Deihimi","doi":"10.22067/jcmr.v9i2.64586","DOIUrl":"https://doi.org/10.22067/jcmr.v9i2.64586","url":null,"abstract":"The isolation of native microorganism that produced biosurfactants in order to oil pollutants bioremediation and hydrophobic oil hydrocarbons availability inter soil texture has become important issues in bioremediation technology. Surfactin is one of the biosurfactants with more application that produced by Bacillus subtilis strains could overcome these problems. Thus in this study, we investigated operon which involved in surfactin biosynthesis and its regulator comQXPA operon due to a high level of surfactin biosynthesis by B. subtilis MJ01 isolated from oil contaminated soil based on comparative genomics approaches. Surfactin operon localized and compared among six genomes of close relative strains and MJ01 indicated that missense point mutations on genes of surfactin operon were existence. These mutations affected NPRS protein AMP-binding domain that responsible to bind amino acid to correct the situation on surfactin peptide ring. It seems that lack of hemolytic and anti-microbial function of MJ01 surfactin was due to the creation of missense mutation and modifications in the surfactin biosynthesis NPRS enzyme structure. Moreover, srf genes expression regulated by comQXPA‏ quorum sensing operon. MJ01 Quorum sensing operon rearrangement showed that part of the comQ gene was extended into comX gene and these genes had overlap region. Results suggested that in MJ01 genome has been occurred specific combination of QS genes organization. Despite high similarity of three genes comQXP‏ among MJ01 with BEST7613 and other subtilis strains group, comA gene showed high identity with spizizenii strains group‏.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"78-83"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46559360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems Biology Analysis of the Key Genes of Surfactin Production in Bacillus subtilis MJ01 (Isolated from Soil Contaminated Oil in South of Iran), Spizizenii, and 168 Isolates 枯草芽孢杆菌MJ01(伊朗南部土壤污染油分离株)、Spizizenii和168株产表面素关键基因的系统生物学分析

Journal of Cell and Molecular Research Pub Date : 2017-12-30 DOI: 10.22067/JCMR.V9I2.66166

Tahereh Deihimi, E. Ebrahimie, A. Niazi, M. Ebrahimi, S. Ayatollahi, A. Tahmasebi, T. Rahimi, M. J. Veshareh

{"title":"Systems Biology Analysis of the Key Genes of Surfactin Production in Bacillus subtilis MJ01 (Isolated from Soil Contaminated Oil in South of Iran), Spizizenii, and 168 Isolates","authors":"Tahereh Deihimi, E. Ebrahimie, A. Niazi, M. Ebrahimi, S. Ayatollahi, A. Tahmasebi, T. Rahimi, M. J. Veshareh","doi":"10.22067/JCMR.V9I2.66166","DOIUrl":"https://doi.org/10.22067/JCMR.V9I2.66166","url":null,"abstract":"Applying microorganism in oil recovery has attracted attentions recently. Surfactin produced by Bacillus subtilis is widely used industrially in a range of industrial applications in pharmecutical and environmental sectors. Little information about molecular mechanism of suffactin compound is available. In this study, we performed promoter and network analysis of surfactin production genes in Bacillus subtilis subsp. MJ01 (isolated from oil contaminated soil in South of Iran), spizizenii and 168. Our analysis revealed that comQ and comX are the genes with sequence alterations among these three strains of Bacillus subtilis and are involved in surfactin production. Promoter analysis indicated that lrp, argR, rpoD, purr and ihf are overrepresented and have the highest number of transcription factor binding sites (TFBs) on the key surfactin production genes in all 3 strains. Also the pattern of TFBs among these three strains was completely different. Interestingly, there is distinct difference between 168, spizizenii and MJ01 in their frequency of TFs that activate genes involve in surfactin production. Attribute weighting algorithms and decision tree analysis revealed ihf, rpoD and flHCD as the most important TF among surfactin production. Network analysis identified two significant network modules. The first one consists of key genes involved in surfactin production and the second module includes key TFs, involved in regulation of surfactin production. Our findings enhance understanding the molecular mechanism of surfactin production through systems biology analysis.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"84-97"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46666200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Non-coding RNAs Could Be New Tools for Cancer Treatment 非编码RNA可能成为癌症治疗的新工具

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/jcmr.v9i1.66961

Atieh Teymoori, M. Teimoori, Madjid Momeni-Moghaddam

{"title":"Non-coding RNAs Could Be New Tools for Cancer Treatment","authors":"Atieh Teymoori, M. Teimoori, Madjid Momeni-Moghaddam","doi":"10.22067/jcmr.v9i1.66961","DOIUrl":"https://doi.org/10.22067/jcmr.v9i1.66961","url":null,"abstract":"For 50 years, the term gene is synonymous with regions of the genome gene that coding by mRNAs and translate to protein. nonetheless, Genome wide Recent studies have revealed that regulating gene expression through degradation or translational inhibition of their point mRNAs and thus attend in a wide variety of physiological and pathological cellular processes including: development, cell proliferation, differentiation, and apoptosis pathways by thousands of regulatory non coding RNA such as lncRNAs and microRNAs. According to a recent survey, it is known this RNAs have vital role in regulation cellular pathways at transcriptional, posttranscriptional and epigenetic levels. These noncoding genes are often aberrantly expressed in a variety of human cancers. However, the biological functions of most ncRNAs remain largely in doubt. In this review, we proved that a remarkable part of the genetic etiology of cancer is imposed by noncoding regulatory sequences. The purpose of this review is aimed to give an outlook of using of noncoding RNA as diagnostic markers and therapeutic targets. These observations emphasized that the recognition of coding genes and Research continued evolution and function of non-coding RNAs for a comprehensive understanding human complex diseases like cancer are essential.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"44-53"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46453563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

RNAseq Reveals Novel and Differentially Expressed Isoforms in Native and Commercial Poultry RNAseq在本地和商业家禽中揭示了新的和差异表达的亚型

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/jcmr.v0i0.59516

A. S. Sadr, M. Nassiri, S. A. Salami, M. Bakhtiarizadeh, M. Tahmoorespur, A. Shafeinia

{"title":"RNAseq Reveals Novel and Differentially Expressed Isoforms in Native and Commercial Poultry","authors":"A. S. Sadr, M. Nassiri, S. A. Salami, M. Bakhtiarizadeh, M. Tahmoorespur, A. Shafeinia","doi":"10.22067/jcmr.v0i0.59516","DOIUrl":"https://doi.org/10.22067/jcmr.v0i0.59516","url":null,"abstract":"The poultry industry occupies an important position in the provision of animal protein. Recently, next generation sequencing technology (RNA-Seq) has become available as a powerful tool to investigate transcriptional profiles for gene expression analysis of many organisms. The main use of RNA-Seq in agriculture species are focusing on finding the immune related genes or pathways by comparison of the whole transcriptome following pathogen challenge. Alternative splicing (AS) is the major fundamental mechanism generating the protein diversity and regulating the gene expression in eukaryotic organism. Identifying genes that are differentially spliced between two groups of RNA-sequencing samples is interesting subject in transcriptome with next-generation sequencing technology in this study used RNA sequencing to comparison isoforms of two breeds. A total of 64,819 transcripts were identified by aligning sequence reads to genome among the evaluated isoforms for expression analysis, 310 were significantly differentially expressed between two breeds, including 251 up-regulated and 59 down-regulated. The KEGG results of up regulated isoforms showed that that no pathway was found significantly different (FDR ≤ 0.05). However, enrichment analysis suggested that seven were over-represented (P-value ≤ 0.05) within the up regulated isoforms. Only one of them functionally related to immune system, natural killer cell mediated cytotoxicity. The results showed genes which are breed-specific expression and the comparative transcriptome analysis help to understand the difference of genetic mechanism.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47122833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of EGFR, MAP2K4 and E2F3 Genes as Targets of miR-141 and Its Association With Immune System Pathway EGFR、MAP2K4和E2F3基因作为miR-141靶点的差异表达及其与免疫系统通路的关联

Journal of Cell and Molecular Research Pub Date : 2017-06-25 DOI: 10.22067/jcmr.v0i0.58965

Soheila Shokrollahzade, Arshad Hosseini, M. Safa, Shamim Sarhadi

{"title":"Differential Expression of EGFR, MAP2K4 and E2F3 Genes as Targets of miR-141 and Its Association With Immune System Pathway","authors":"Soheila Shokrollahzade, Arshad Hosseini, M. Safa, Shamim Sarhadi","doi":"10.22067/jcmr.v0i0.58965","DOIUrl":"https://doi.org/10.22067/jcmr.v0i0.58965","url":null,"abstract":"MicroRNAs by their structural complementarity capabilities have canonical roles in gene regulation. In this paper; we investigate expression of EGFR, MAP2K4 and E2F3 genes targeted by miR-141, a member of miR-200 family. EGFR, MAP2K4 and E2F3 were predicted as the potential targets of mir-141 by using online miRNA bioinformatics tools. MCF-7 cells were transfected with mir-141-precursor and inhibitor vectors. Expression of miR-141 and target genes was determined by using qRT-PCR. To see the most relevant pathways regulated by miR-141, we constructed two separate networks by NetworkAnalyst and enriched list of underlying genes by Enrichment analysis tools. The expression changes of all three predicted targets were higher in transfected cells with anti-mir-141 vector, compared with the control untransfected cells. By contrast, in transfected cells with pre-mir 141, we did not see significant expression changes in EGFR, E2F3 and MAP2K4. List of genes in total networks as well as explored functional modules were enriched separately. Enrichment analysis shows that immune system pathway has the strongest relationship with the proteins potentially targeted by miR-141. The present study demonstrated potential role of miR-141 in regulation of EGFR, MAP2K4 and E2F3 expression and suggested innate immunity pathways as the key pathway through which this regulatory network contributes to breast cancer development.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"9 1","pages":"6-15"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48846106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1