Journal of Cell and Molecular Research最新文献_第3页

Evaluation of IFN-γ and T-bet Expression Levels as Possible Molecular Markers of Schizophrenia IFN-γ和T-bet表达水平作为精神分裂症可能分子标志物的评价

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.85426

Roghaye Lorestani, Sohrab Boozarpour, S. Alijanpour, L. Ahangar

{"title":"Evaluation of IFN-γ and T-bet Expression Levels as Possible Molecular Markers of Schizophrenia","authors":"Roghaye Lorestani, Sohrab Boozarpour, S. Alijanpour, L. Ahangar","doi":"10.22067/JCMR.V11I2.85426","DOIUrl":"https://doi.org/10.22067/JCMR.V11I2.85426","url":null,"abstract":"Schizophrenia is an irritating mental disorder that affects around 1% of the world's population. The immune system contributes to the onset of the disease, particularly through production and secretion of some cytokines. In patients with schizophrenia, the balance of Th1 to Th2 ratio is often altered. In the present study, we investigated these changes by measuring the gene expression levels of IFN-γ and T-bet as Th1 indicators, as well as IL-4 and GATA-3 as representatives for Th2. Blood samples of schizophrenic patients (n=25) and healthy individuals (n=10) were obtained. Total RNA was extracted from leukocytes and cDNA synthesis was performed based on provided protocols. Real-time PCR technique was utilized for the assessment of gene expression levels. Results indicated a significant increase in the expression of IFN-γ and its transcription factor, T-bet, while IL-4 gene expression was reduced significantly. The expression level of GATA-3 gene revealed no meaningful changes. Altogether, results confirmed the relative shift of Th1 to Th2 status in the patient with schizophrenia and re-emphasize the importance of the inflammatory events in the incidence of the disease. Moreover, a new index was introduced based on the IFN-γ and T-bet genes expression, which can determine healthy condition with total accuracy of 79%.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"82-89"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42357858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography 离子对反相高效液相色谱法快速分析cDNA合成

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.83212

M. Matin, D. Hornby

引用次数: 0

In silico Analysis of Determinant Factors in Microbial Protease Thermostability 微生物蛋白酶热稳定性决定因素的计算机模拟分析

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.84965

P. Farrokh, F. Salimi

{"title":"In silico Analysis of Determinant Factors in Microbial Protease Thermostability","authors":"P. Farrokh, F. Salimi","doi":"10.22067/JCMR.V11I2.84965","DOIUrl":"https://doi.org/10.22067/JCMR.V11I2.84965","url":null,"abstract":"Thermostable proteases are one of the pivotal enzymatic groups which play fundamental roles in biotechnologyrelated industries. The identification of bacterial thermostable enzymes through screening programs is a time and cost consuming process. So, extensive bioinformatics and experimental studies have been conducted to reveal thermo stabilizing factors. The current study was aimed to evaluate distinctive indicators among 33 thermostable and 10 mesostable proteolytic enzymes. The frequency of individual amino acids, aliphatic indexes, melting temperatures, isoelectric points, as well as, the frequency of AXXXA and GXXXG motifs were determined and compared among these enzymes. In addition, types of proteolytic enzymes and their active sites were assigned. Moreover, the frequency of alpha helixes, polar surface regions, and packing volumes of these enzymes with the known structures were characterized. Results showed that the frequency of Ala and AXXXA motifs were significantly higher in thermostable proteolytic enzymes, while they possess lower contents of Met, His, Lys and Leu in comparison to mesostable enzymes (P<0.05). According to statistical analysis, thermostable proteolytic enzymes indicated meaningful lower packing volumes than mesostable enzymes (P<0.05). Findings of the current study in addition to more detailed investigations on the thermostability mechanisms of various protein families are essential for designing more efficient industrial enzymes with functional properties at high temperatures.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"66-81"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44685397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin E Pretreatment of Mesenchymal Stem Cells: The Interplay of Oxidative Stress and Inflammation 维生素E预处理间充质干细胞:氧化应激和炎症的相互作用

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.85668

S. Mehrzad, Sepideh Hosseini, Madjid Momeni-Moghaddam, Moien Farshchian, H. Hassanzadeh, M. Mirahmadi, F. Sadeghifar, H. Bidkhori

{"title":"Vitamin E Pretreatment of Mesenchymal Stem Cells: The Interplay of Oxidative Stress and Inflammation","authors":"S. Mehrzad, Sepideh Hosseini, Madjid Momeni-Moghaddam, Moien Farshchian, H. Hassanzadeh, M. Mirahmadi, F. Sadeghifar, H. Bidkhori","doi":"10.22067/JCMR.V11I2.85668","DOIUrl":"https://doi.org/10.22067/JCMR.V11I2.85668","url":null,"abstract":"Oxidative stress occurs as a result of breaking down the balance between oxidants (e.g., reactive oxygen species (ROS)) and antioxidants in cells. Several studies have shown that there is a close relationship between oxidative stress and inflammation at the sites of injury. Mesenchymal stem cells (MSCs) are exposed to endogenous and exogenous oxidants generated during their ex vivo expansion or following in vivo transplantation. α-tocopherol (vitamin E) is a fat-soluble compound known for its anti-oxidant and anti-inflammatory properties. In many studies, the immunomodulatory effects of vitamin E have been observed in vivo. This study aimed to determine whether pretreatment of MSCs with antioxidants like vitamin E, will enhance the anti-inflammatory and immunomodulatory properties of these cells. For this purpose, adipose-derived MSCs (ASCs) were treated with vitamin E (600 μM) for 48 h. Quantitative PCR (qPCR) experiments were performed to evaluate the expression of genes related to inflammation (IL-1β, IL-6, IL-17, IL-10) or immunomodulation (TSG-6, COX-2, TDO2, TGF-β1). Results indicated that vitamin E significantly increased the expression of COX-2, TSG-6, and IL-1β genes at the mRNA level compared with the control group, while it significantly decreased IL-6 and TGF-β expressions. No effect was observed for IL-17, IL-10, and TDO2 genes. These results suggest that in vitro preconditioning of ASCs with vitamin E may allow the cells to improve their anti-inflammatory and immunoregulatory capacities. Vitamin E pretreatment could lead to the improvement of their therapeutic abilities in conditions that are influenced by oxidative stress.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"99-107"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48278482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Immunosuppressive Effects of Human Chorionic Gonadotropin (hCG) on Mesenchymal Stromal Cells 人绒毛膜促性腺激素(hCG)对间充质基质细胞的免疫抑制作用

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.85667

Sepideh Hosseini, S. Mehrzad, H. Hassanzadeh, H. Bidkhori, M. Mirahmadi, Madjid Momeni-Moghaddam, F. Sadeghifar, M. Farshchian

{"title":"Immunosuppressive Effects of Human Chorionic Gonadotropin (hCG) on Mesenchymal Stromal Cells","authors":"Sepideh Hosseini, S. Mehrzad, H. Hassanzadeh, H. Bidkhori, M. Mirahmadi, Madjid Momeni-Moghaddam, F. Sadeghifar, M. Farshchian","doi":"10.22067/JCMR.V11I2.85667","DOIUrl":"https://doi.org/10.22067/JCMR.V11I2.85667","url":null,"abstract":"Mesenchymal stem/stromal cells (MSCs) as one of the most important types of adult stem cells secrete a variety of immunomodulatory cytokines. However, their immunomodulatory features strongly depend on the molecular cross-talk between cells and the surrounding microenvironment. Hence, some strategies were proposed to empower their beneficial effects during cell-therapeutic procedures to avoid confusing results. Licensing the cells with chemical compounds could be considered as one of the most applicable methods for induction of anti-inflammatory status in the cells. Human chorionic gonadotropin (hCG) is a pregnancy related hormone which has been shown to be essential for the establishment of a successful pregnancy. HCG supports the implantation of fetus in the maternal endometrium, due to its immunomodulatory effects. Moreover, the regulatory role of hCG has been previously mentioned in case of some autoimmune-based diseases. In the present study, the capacity of this hormone for induction of different immune-encountered genes expression was examined in primary cultures of human adipose tissue derived mesenchymal stem cells (Ad-MSCs). In this regard, Ad-MSCs were exposed to 10 IU of hCG for 72 hours. Molecular studies via quantitative Real-time PCR (qRT-PCR) experiments were performed to detect gene expression modifications based on the application of SYBR Green as the fluorescent dye and in comparison to the RPLP0 as the housekeeping gene. Results confirmed that hCG significantly upregulated TSG-6, TGF-β1, IL-1β and IL-6 expression levels comparing with the control group, while it downregulates COX-2 expression, and had no statistically significant effects on IL-10 andTDO2. In conclusion, priming Ad-MSCs with hCG may enhance the proliferation and immunoregulatory potential of these cells, although it needs further investigations to reveal involved molecular pathways.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"90-98"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45719178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Production of Silver Nanoparticles by Marigold Extract 万寿菊提取物制备纳米银

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.84974

Zohreh Sohrabi Nezhad, H. Marashi, N. Moshtaghi

引用次数: 1

Cost-effective Strategies for Depletion of Endogenous Extracellular Vesicles from Fetal Bovine Serum 从胎牛血清中去除内源性细胞外囊泡的成本效益策略

Journal of Cell and Molecular Research Pub Date : 2020-03-01 DOI: 10.22067/JCMR.V11I2.85209

Azadeh Haghighitalab, M. Matin, Fatemeh Khakrah, A. Asoodeh, A. Bahrami

{"title":"Cost-effective Strategies for Depletion of Endogenous Extracellular Vesicles from Fetal Bovine Serum","authors":"Azadeh Haghighitalab, M. Matin, Fatemeh Khakrah, A. Asoodeh, A. Bahrami","doi":"10.22067/JCMR.V11I2.85209","DOIUrl":"https://doi.org/10.22067/JCMR.V11I2.85209","url":null,"abstract":"Despite the prominent therapeutic potentials of stem cells, their use in cell therapy has been challenged with some unreproducible and inconsistent outcomes in addition to the risk of rejection and tumorigenesis. Gaining novel insights to the importance of the conditioned medium, secretory factors and extracellular vesicles as the functional components of the cultured stem cells, suggested the idea of substituting the cells with their cell-free counterparts. Biological properties of these products are influenced by the cues received from their microenvironment. Hence, providing optimal and fully defined culture conditions is essential for their preparation. Fetal bovine serum (FBS), one of the most routine supplements of cell culture, is enriched by endogenous extracellular vesicles (EVs). These EVs will affect the yield, purity and functional features of the cell-free products. Here, we endeavored to examine and compare three different methods including ultrasonication, ultrafiltration and polymer-based precipitation, to deplete EVs from FBS. We chose easy to perform and fast methods with the capacity for high-throughput applications. Based on our observations, although all examined methods were able to deplete EVs from FBS to some extent, polymer-based precipitation could be considered as the method of choice with minimal consequences on the biological requirements of FBS to support cell growth and characteristics. Due to similarities between FBS and some other biological solutions, this strategy would be suitable for EV-depletion from other liquids with high concentrations of proteins and nutrients. Moreover, it could be applied for preparation of optimal culture conditions for nanoparticle applications.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"42-54"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46039703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies LEPR (853A>G和511A>G)转换可能增加特发性复发性流产:基于病例对照和计算机研究的证据

Journal of Cell and Molecular Research Pub Date : 2019-09-01 DOI: 10.22067/JCMR.V11I1.81426

A. Colagar, Masomeh Salehi-Doon

{"title":"The LEPR (853A>G and 511A>G) Transitions may Enhance Idiopathic Recurrent Miscarriage: Evidences Based on Case-control and in silico Studies","authors":"A. Colagar, Masomeh Salehi-Doon","doi":"10.22067/JCMR.V11I1.81426","DOIUrl":"https://doi.org/10.22067/JCMR.V11I1.81426","url":null,"abstract":"Previous studies in human leptin receptor protein (LEPR) signaling are important in the establishment of fetal growth. Idiopathic recurrent miscarriage (IRM) may be the result of abnormal placental and fetal development. Thus single nucleotide polymorphisms (SNPs) of LEPR might be associated with IRM. In our case-control study, which conducted from 2017 to 2018 at the Milad Sari Genetic Detection Center and Razi Hospital (Ghaemshahr, Iran), 140 samples, including 70 cases with history of three or more IRM as before the 22nd week of gestation, and 70 controls with at least two live births and no history of pathologic pregnancies during reproductive period were studied. Polymorphisms of maternal LEPR 853A>G and 511A>G were assessed by PCR-RFLP and SSCP, respectively. Results showed that 853A>G SNP, contained frequent genotype AG (p= 0.002; OR= 0.391; 95% CI= 0.154-0.664) and G allele (p= 0.003; OR= 0.125; 95% CI= 0.032–0.489), revealed a significant protective association with IRM. Primary screening of 511A>G showed that 63 case-samples were AG genotype. PCR directed sequence showed this SNP contained frequent genotype for AG (p= 0.001; OR= 0.57; 95% CI= 0.22-0.147) and G allele (p= 0.006; OR= 0.34; 95% CI= 0.008–0.149), revealed a significant protective association with IRM. Based on our findings, LEPR (853A>G and 511A>G) gene transitions not only might enhance IRM but also could be useful genetic markers in susceptibility and severity of recurrent miscarriage.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"11 1","pages":"23-36"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44249123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I 葫芦霉素D、E和I诱导大肠癌癌症细胞系AHR基因表达

Journal of Cell and Molecular Research Pub Date : 2019-03-01 DOI: 10.22067/JCMR.V10I2.74806

Younes Aftabi, H. Zarredar, M. Sheikhi, Z. Khoshkam, A. Colagar

{"title":"Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I","authors":"Younes Aftabi, H. Zarredar, M. Sheikhi, Z. Khoshkam, A. Colagar","doi":"10.22067/JCMR.V10I2.74806","DOIUrl":"https://doi.org/10.22067/JCMR.V10I2.74806","url":null,"abstract":"Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor and its induction may result in suppressing of cell proliferation in colorectal cancer (CRC). Cucurbitacin D (CucD), E (CucE) and I (CucI) are plant derived metabolites that inhibit cancer cells. This study aimed to evaluate the possible potency of the cucurbitacins for activation of AHR expression in CRC cell lines SW-480 and HT-29. The MTT assay was used to find the LC50 value of the metabolites in the cell lines. Afterward, the cells incubated with the LC50 concentrations and AHR-mRNA expression assessed using RT-PCR. The LC50 values of CucD, CucE, and CucI were 4.5, 6.8, and 3.8 μM in HT-29 cell line and 35, 19, 17.5 μM in SW-480 cells respectively. The SW-480 cells were more resistant against Cucs in comparison with HT-29 cells and all three Cucs cause to more AHR-mRNA expression in HT-29 cells. CucE had the lowest effect on AHR-mRNA expression in the cell lines and CucI was a common metabolite for both HT-29 and SW-480 cells, which showed the lowest LC50 value (the highest toxicity) and the highest effect on AHR-mRNA expression. CucI may have a potential to be used as an activator of AHR expression in CRC studies.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"10 1","pages":"67-75"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44269882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Global Analysis of Gene Expression and Identification of Modules in Echinacea purpurea Using Systems Biology Approach 应用系统生物学方法对紫锥菊基因表达和模块鉴定的全局分析

Journal of Cell and Molecular Research Pub Date : 2018-09-30 DOI: 10.22067/JCMR.V10I1.67357

A. Tahmasebi, F. Aram, H. Pakniyat, A. Niazi, E. Tavakol, E. Ebrahimie

{"title":"Global Analysis of Gene Expression and Identification of Modules in Echinacea purpurea Using Systems Biology Approach","authors":"A. Tahmasebi, F. Aram, H. Pakniyat, A. Niazi, E. Tavakol, E. Ebrahimie","doi":"10.22067/JCMR.V10I1.67357","DOIUrl":"https://doi.org/10.22067/JCMR.V10I1.67357","url":null,"abstract":"Co-expression analysis is a useful tool to analysis data and detection of genes that act in the same pathway or biological process. Echinacea purpurea is one of the most important medicinal plant of the Asteraceae family that is known as antioxidative and antiviral agent. Despite medicinal importance of E. purpurea, very few reports are available for metabolic mechanisms in this plant. With the aim to elucidate the gene expression profiling and identification of modules in E. purpurea, we performed a systems biology analysis on publicly available transcriptome data. Gene ontology and KEGG pathway enrichment analysis revealed that the unigenes were highly related to the cellular process, primary metabolic process, carbon metabolism and biosynthesis of antibiotics. The coexpression networks divided genes into multiple modules. Of these, module M2 associated with secondary metabolic process. Moreover, a total of 47 transcription factor families such as bHLH, bZIP, C2H2, MYB and WRKY in modules were identified. These findings can provide an overall picture for better understanding the gene expression patterns and common transcriptional mechanisms in E. purpurea.","PeriodicalId":30607,"journal":{"name":"Journal of Cell and Molecular Research","volume":"10 1","pages":"18-26"},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41510993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3