ACS Measurement Science Au最新文献

{"title":"Molecular Stress Response of Mitochondria during Electrostimulation Evoking Stem Cell Differentiation Revealed by Fluorescence Imaging Combined with SERS Spectra.","authors":"Jiafeng Wang, Xiaozhang Qu, Zhimin Zhang, Xiuping Meng, Guohua Qi","doi":"10.1021/acsmeasuresciau.5c00005","DOIUrl":"10.1021/acsmeasuresciau.5c00005","url":null,"abstract":"<p><p>Stem cells are a class of multipotential cells with the capability of self-replication, which can differentiate into multiple functional cells under extra stimulus. The differentiation of stem cells has important implications for tissue regeneration. Therefore, controllable regulation of dental pulp stem cell (DPSC) behaviors is critical for repairment and regeneration of damaged teeth tissues. Rapid promotion of DPSCs, directed differentiation, and revealing molecular events within the organelle level during the cell differentiation process are in great demand for regeneration of teeth, which remains a great challenge. Herein, we developed a highly effective and uncomplicated stimulation platform to promote the DPSCs for odontogenic differentiation based on impulse electrical stimulation and revealed the molecular stress response of mitochondria during cell differentiation based on fluorescence imaging combined with surface-enhanced Raman spectroscopy (SERS). Our approach can greatly shorten the DPSC differentiation time from usually more than 20 days to only about 3 days under 0.8 V for 5 min every day than drug stimulation. Notably, the glycogen and adenosine triphosphate levels within mitochondria were apparently elevated, which is conducive to improving the progression of cell differentiation. Simultaneously, the expression of mitofusin1 and mitofusin2 within mitochondria was significantly down-regulated during the differentiation process. Mechanistically, the molecular insights into mitochondria within DPSCs were clearly revealed through SERS spectra. It demonstrated that the expression of phenylalanine was significantly reduced, whereas the contents of tryptophan within mitochondria were promoted during the cell differentiation process. This study provides a comprehensive and clinically feasible strategy for the rapid promotion of DPSCs-directed differentiation and reveals the molecular dynamic changes within mitochondria, which broadens the biomedical cognition of electrical stimulation for dental pulp stem cell differentiation and provides a potential application for teeth tissue regeneration in the future.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 3","pages":"294-303"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dual-Function Microelectronic Fibers for pH and Temperature Sensing: Toward In Vivo and Wearable Applications","authors":"Mahiro Kubo,&nbsp;Mayuko Abe,&nbsp;Etienne Le Bourdonnec,&nbsp;Sheau-Chyi Wu,&nbsp;To-En Hsu,&nbsp;Takao Inoue and Yuanyuan Guo*,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0009210.1021/acsmeasuresciau.4c00092","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00092https://doi.org/10.1021/acsmeasuresciau.4c00092","url":null,"abstract":"<p >Temperature plays a crucial role in biological functions in normal physiological and pathological states and is intricately linked with chemical dynamics <i>in vivo</i> at the cellular, circuit, and system levels. Despite advances in temperature measurement technologies for internal monitoring, systems capable of simultaneously tracking localized temperature and chemical changes are still underdeveloped. In this study, we introduce dual-sensing hybrid fibers with a miniature footprint of &lt;400 μm in diameter, fabricated using the thermal drawing process. These fibers exhibit precise temperature sensitivity, detecting changes as small as 0.5 °C, and demonstrate highly sensitive and reversible pH detection, a critical physiological parameter. Furthermore, through laser micromachining and surface functionalization, we highlight the potential of these fibers for wearable applications in dual pH and temperature sensing. This innovative dual-sensing technology offers a versatile platform for probing temperature and chemical signaling <i>in vivo</i> and wearable applications, with significant implications for therapeutic development and a deeper understanding of biological processes in various environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"208–215 208–215"},"PeriodicalIF":4.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dual-Function Microelectronic Fibers for pH and Temperature Sensing: Toward <i>In Vivo</i> and Wearable Applications.","authors":"Mahiro Kubo, Mayuko Abe, Etienne Le Bourdonnec, Sheau-Chyi Wu, To-En Hsu, Takao Inoue, Yuanyuan Guo","doi":"10.1021/acsmeasuresciau.4c00092","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00092","url":null,"abstract":"<p><p>Temperature plays a crucial role in biological functions in normal physiological and pathological states and is intricately linked with chemical dynamics <i>in vivo</i> at the cellular, circuit, and system levels. Despite advances in temperature measurement technologies for internal monitoring, systems capable of simultaneously tracking localized temperature and chemical changes are still underdeveloped. In this study, we introduce dual-sensing hybrid fibers with a miniature footprint of <400 μm in diameter, fabricated using the thermal drawing process. These fibers exhibit precise temperature sensitivity, detecting changes as small as 0.5 °C, and demonstrate highly sensitive and reversible pH detection, a critical physiological parameter. Furthermore, through laser micromachining and surface functionalization, we highlight the potential of these fibers for wearable applications in dual pH and temperature sensing. This innovative dual-sensing technology offers a versatile platform for probing temperature and chemical signaling <i>in vivo</i> and wearable applications, with significant implications for therapeutic development and a deeper understanding of biological processes in various environments.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"208-215"},"PeriodicalIF":4.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multivariate Strategy for Understanding Soil Features from Rare-Earth Element Profiles: A Focus on Data Normalization","authors":"Marcella Barbera,&nbsp;Sara Gariglio,&nbsp;Cristina Malegori*,&nbsp;Paolo Oliveri,&nbsp;Filippo Saiano,&nbsp;Riccardo Scalenghe and Daniela Piazzese,&nbsp;","doi":"10.1021/acsmeasuresciau.4c0008410.1021/acsmeasuresciau.4c00084","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00084https://doi.org/10.1021/acsmeasuresciau.4c00084","url":null,"abstract":"<p >The interest in assessing the behavior of rare-earth elements (REE) in the natural environment is constantly increasing due to their numerous applications in both environmental and technological fields. However, current methodologies for analyzing REE distributions are based on normalization of REE concentration profiles to lithological values, potentially resulting in different outcomes depending on which lithological values are used for normalization, affecting the interpretability of the data. The present work proposes an alternative approach for analyzing REE concentration profiles by applying principal component analysis (PCA) to create REE chemometric maps. The data compression allows the visualization of the REE distribution using a red-green-blue (RGB) color scale (PC1 = red channel; PC2 = green channel; PC3 = blue channel) directly on a geographical map, reflecting the chemical properties of rare-earth elements. This highlights similarities and differences in the compositional REE distribution of natural soils, facilitating the interpretability of REE data and potentially leading to new insights related to seemingly unrelated samples. Additionally, PCA applied to soil variables correlates with REE patterns and provides deeper insights into soil properties in an unsupervised manner, enhancing the interpretation of soil characteristics and implementing the ability to monitor environmental changes and study soil evolution processes. Of particular significance is the fact that applying the proposed methodology to non-normalized data yields results that are consistent with those derived from normalized data sets. Therefore, this approach not only overcomes normalization challenges but also supports the classical approach from a new methodological perspective, paving the way for broader applications.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"189–198 189–198"},"PeriodicalIF":4.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.4c00084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multivariate Strategy for Understanding Soil Features from Rare-Earth Element Profiles: A Focus on Data Normalization.","authors":"Marcella Barbera, Sara Gariglio, Cristina Malegori, Paolo Oliveri, Filippo Saiano, Riccardo Scalenghe, Daniela Piazzese","doi":"10.1021/acsmeasuresciau.4c00084","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.4c00084","url":null,"abstract":"<p><p>The interest in assessing the behavior of rare-earth elements (REE) in the natural environment is constantly increasing due to their numerous applications in both environmental and technological fields. However, current methodologies for analyzing REE distributions are based on normalization of REE concentration profiles to lithological values, potentially resulting in different outcomes depending on which lithological values are used for normalization, affecting the interpretability of the data. The present work proposes an alternative approach for analyzing REE concentration profiles by applying principal component analysis (PCA) to create REE chemometric maps. The data compression allows the visualization of the REE distribution using a red-green-blue (RGB) color scale (PC1 = red channel; PC2 = green channel; PC3 = blue channel) directly on a geographical map, reflecting the chemical properties of rare-earth elements. This highlights similarities and differences in the compositional REE distribution of natural soils, facilitating the interpretability of REE data and potentially leading to new insights related to seemingly unrelated samples. Additionally, PCA applied to soil variables correlates with REE patterns and provides deeper insights into soil properties in an unsupervised manner, enhancing the interpretation of soil characteristics and implementing the ability to monitor environmental changes and study soil evolution processes. Of particular significance is the fact that applying the proposed methodology to non-normalized data yields results that are consistent with those derived from normalized data sets. Therefore, this approach not only overcomes normalization challenges but also supports the classical approach from a new methodological perspective, paving the way for broader applications.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"189-198"},"PeriodicalIF":4.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"White Light Transmission Spectroscopy for Rapid Quality Control Imperfection Identification in Nanoimprinted Surface-Enhanced Raman Spectroscopy Substrates","authors":"Mike Hardy*,&nbsp;Hin On Martin Chu,&nbsp;Serene Pauly,&nbsp;Katie F. Cavanagh,&nbsp;Breandán J.F. Hill,&nbsp;Jason Wiggins,&nbsp;Alina Schilling,&nbsp;Pola Goldberg Oppenheimer,&nbsp;Liam M. Grover,&nbsp;Richard J. Winfield,&nbsp;Jade N. Scott,&nbsp;Matthew D. Doherty,&nbsp;Ryan McCarron,&nbsp;William R. Hendren,&nbsp;Paul Dawson and Robert M. Bowman,&nbsp;","doi":"10.1021/acsmeasuresciau.5c0000310.1021/acsmeasuresciau.5c00003","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00003https://doi.org/10.1021/acsmeasuresciau.5c00003","url":null,"abstract":"<p >Miniaturized biomedical sensor development requires improvements in lithographic processes in terms of cost and scalability. Of particular promise is nanoimprint lithography (NIL), but this can suffer from a lack of high-fidelity pattern reproducibility between master and imprinted substrates. Herein, we present a multidisciplinary investigation into gold- and iron-coated NIL sensors including custom optics and spectroscopy, scanning probe microscopy, and data analysis insights. Polyurethane NIL-made nanodome arrays were interrogated with white light transmission spectroscopy, coupled with principal component analysis (PCA) to investigate potential offsets in the photon-substrate plane interaction angle, an imperfection in NIL substrates. Large-angle mismatches (2–10°) were found to be easily discernible by PCA with statistically significant differences (<i>p</i> = 0.05). Unexpected dips in some spectra are postulated to be due to interacting localized and propagating plasmon polaritons, which is supported with a coupled two-oscillator model. General insights are made regarding the interpretation of PCA loadings, which should be related to physical phenomena, and where maximum variance is not necessarily the most meaningful criterion. Smaller angles (&lt;1°) show no significant differences with overlapping confidence intervals in PCA space. Surface-enhanced Raman spectroscopy (SERS) measurements on gold-coated nanodomes returned relative standard deviations of 6–10% via analysis of gelatin, which is of interest as a nasal lining approximation. Interestingly, nanodomes coated in iron produced small, but useful SERS enhancements, which was subsequently interrogated via scanning thermal probe microscopy showing temperature increases of up to 5 °C over the area of one nanostructure (∼1 μm²). Nanostructures remained intact despite the surprising large local temperature increase relative to a gold-coated comparison sample (∼2 °C). The current study provides a framework for the rapid and accurate quality control assessment of imperfections in NIL-produced nanostructures for sensing applications in SERS and surface plasmon resonance, which may need precisely fabricated nanostructures.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"250–263 250–263"},"PeriodicalIF":4.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.5c00003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"White Light Transmission Spectroscopy for Rapid Quality Control Imperfection Identification in Nanoimprinted Surface-Enhanced Raman Spectroscopy Substrates.","authors":"Mike Hardy, Hin On Martin Chu, Serene Pauly, Katie F Cavanagh, Breandán J F Hill, Jason Wiggins, Alina Schilling, Pola Goldberg Oppenheimer, Liam M Grover, Richard J Winfield, Jade N Scott, Matthew D Doherty, Ryan McCarron, William R Hendren, Paul Dawson, Robert M Bowman","doi":"10.1021/acsmeasuresciau.5c00003","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00003","url":null,"abstract":"<p><p>Miniaturized biomedical sensor development requires improvements in lithographic processes in terms of cost and scalability. Of particular promise is nanoimprint lithography (NIL), but this can suffer from a lack of high-fidelity pattern reproducibility between master and imprinted substrates. Herein, we present a multidisciplinary investigation into gold- and iron-coated NIL sensors including custom optics and spectroscopy, scanning probe microscopy, and data analysis insights. Polyurethane NIL-made nanodome arrays were interrogated with white light transmission spectroscopy, coupled with principal component analysis (PCA) to investigate potential offsets in the photon-substrate plane interaction angle, an imperfection in NIL substrates. Large-angle mismatches (2-10°) were found to be easily discernible by PCA with statistically significant differences (<i>p</i> = 0.05). Unexpected dips in some spectra are postulated to be due to interacting localized and propagating plasmon polaritons, which is supported with a coupled two-oscillator model. General insights are made regarding the interpretation of PCA loadings, which should be related to physical phenomena, and where maximum variance is not necessarily the most meaningful criterion. Smaller angles (<1°) show no significant differences with overlapping confidence intervals in PCA space. Surface-enhanced Raman spectroscopy (SERS) measurements on gold-coated nanodomes returned relative standard deviations of 6-10% via analysis of gelatin, which is of interest as a nasal lining approximation. Interestingly, nanodomes coated in iron produced small, but useful SERS enhancements, which was subsequently interrogated via scanning thermal probe microscopy showing temperature increases of up to 5 °C over the area of one nanostructure (∼1 μm²). Nanostructures remained intact despite the surprising large local temperature increase relative to a gold-coated comparison sample (∼2 °C). The current study provides a framework for the rapid and accurate quality control assessment of imperfections in NIL-produced nanostructures for sensing applications in SERS and surface plasmon resonance, which may need precisely fabricated nanostructures.</p>","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"250-263"},"PeriodicalIF":4.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celebrating 5 Years of the ACS Au Journal Family.","authors":"Paul D Goring, Amelia Newman, Christopher W Jones, Shelley D Minteer","doi":"10.1021/acsmeasuresciau.5c00017","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00017","url":null,"abstract":"","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"157-159"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celebrating 5 Years of the ACS Au Journal Family","authors":"Paul D. Goring,&nbsp;Amelia Newman,&nbsp;Christopher W. Jones* and Shelley D. Minteer*,&nbsp;","doi":"10.1021/acsmeasuresciau.5c0001710.1021/acsmeasuresciau.5c00017","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.5c00017https://doi.org/10.1021/acsmeasuresciau.5c00017","url":null,"abstract":"","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 2","pages":"157–159 157–159"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.5c00017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Alexandros Ch. Lazanas,&nbsp; and ,&nbsp;Mamas I. Prodromidis*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"5 1","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsmeasuresciau.5c00007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144400503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}