ACS Measurement Science Au最新文献_第9页

Colorimetric Signal Readout for the Detection of Volatile Organic Compounds Using a Printable Glass-Based Dielectric Barrier Discharge-Type Helium Plasma Detector 使用可印刷玻璃基介电阻挡放电型氦等离子体探测器检测挥发性有机化合物的比色信号读出

ACS Measurement Science Au Pub Date : 2023-05-30 DOI: 10.1021/acsmeasuresciau.3c00012

Jingqin Mao, Longze Liu, Yahya Atwa, Junming Hou, Zhenxun Wu and Hamza Shakeel*,

{"title":"Colorimetric Signal Readout for the Detection of Volatile Organic Compounds Using a Printable Glass-Based Dielectric Barrier Discharge-Type Helium Plasma Detector","authors":"Jingqin Mao, Longze Liu, Yahya Atwa, Junming Hou, Zhenxun Wu and Hamza Shakeel*, ","doi":"10.1021/acsmeasuresciau.3c00012","DOIUrl":"10.1021/acsmeasuresciau.3c00012","url":null,"abstract":"In this paper, we report on a printable glass-based manufacturing method and a new proof-of-concept colorimetric signal readout scheme for a dielectric barrier discharge (DBD)-type helium plasma photoionization detector. The sensor consists of a millimeter-sized glass chamber manufactured using a printable glass suspension. Plasma inside the chip is generated using a custom-built power supply (900 V and 83.6 kHz), and the detector uses ∼5 W of power. Our new detection scheme is based on detecting the change in the color of plasma after the introduction of target gases. The change in color is first captured by a smartphone camera as a video output. The recorded video is then processed and converted to an image light intensity vs retention time plot (gas chromatogram) using three standard color space models (red, green, blue (RGB), hue, saturation, lightness (HSL), and hue, saturation, value (HSV)) with RGB performing the best among the three models. We successfully detected three different categories of volatile organic compounds using our new detection scheme and a 30-m-long gas chromatography column: (1) straight-chain alkanes (n-pentane, n-hexane, n-heptane, n-octane, and n-nonane), (2) aromatics (benzene, toluene, and ethylbenzene), and (3) polar compounds (acetone, ethanol, and dichloromethane). The best limit of detection of 10 ng was achieved for benzene at room temperature. Additionally, the device showed excellent performance for different types of sample mixtures consisting of three and five compounds. Our new detector readout method combined with our ability to print complex glass structures provides a new research avenue to analyze complex gas mixtures and their components.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 4","pages":"287–300"},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Versatile and Easy Method to Calibrate a Two-Compartment Flow Cell for Differential Electrochemical Mass Spectrometry Measurements 一个通用的和简单的方法来校准一个双室流动电池的微分电化学质谱测量

ACS Measurement Science Au Pub Date : 2023-05-19 DOI: 10.1021/acsmeasuresciau.3c00009

Christoph J. Bondue, Marc T. M. Koper and Kristina Tschulik*,

{"title":"A Versatile and Easy Method to Calibrate a Two-Compartment Flow Cell for Differential Electrochemical Mass Spectrometry Measurements","authors":"Christoph J. Bondue, Marc T. M. Koper and Kristina Tschulik*, ","doi":"10.1021/acsmeasuresciau.3c00009","DOIUrl":"10.1021/acsmeasuresciau.3c00009","url":null,"abstract":"Online techniques for the quantitative analysis of reaction products have many advantages over offline methods. However, owing to the low product formation rates in electrochemical reactions, few of these techniques can be coupled to electrochemistry. An exception is differential electrochemical mass spectrometry (DEMS), which gains increasing popularity not least because of its high time resolution in the sub-second regime. DEMS is often combined with a dual thin-layer cell (a two-compartment flow cell), which helps to mitigate a number of problems that arise due to the existence of a vacuum|electrolyte interface. However, the efficiency with which this cell transfers volatile reaction products into the vacuum of the mass spectrometer is far below 100%. Therefore, a calibration constant that considers not only the sensitivity of the DEMS setup but also the transfer efficiency of the dual thin-layer cell is needed to translate the signals observed in the mass spectrometer into electrochemical product formation rates. However, it can be challenging or impossible to design an experiment that yields such a calibration constant. Here, we show that the transfer efficiency of the dual thin-layer cell depends on the diffusion coefficient of the analyte. Based on this observation, we suggest a two-point calibration method. That is, a plot of the logarithm of the transfer efficiencies determined for H2 and O2 versus the logarithm of their diffusion coefficients defines a straight line. Extrapolation of this line to the diffusion coefficient of another analyte yields a good estimate of its transfer efficiency. This is a versatile and easy calibration method, because the transfer efficiencies of H2 and O2 are readily accessible for a large range of electrode–electrolyte combinations.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 4","pages":"277–286"},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fc/50/tg3c00009.PMC10436368.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10046178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Strategies for Overcoming the Single-Molecule Concentration Barrier 克服单分子浓度障碍的策略。

ACS Measurement Science Au Pub Date : 2023-05-09 DOI: 10.1021/acsmeasuresciau.3c00002

David S. White, Mackinsey A. Smith, Baron Chanda and Randall H. Goldsmith*,

{"title":"Strategies for Overcoming the Single-Molecule Concentration Barrier","authors":"David S. White, Mackinsey A. Smith, Baron Chanda and Randall H. Goldsmith*, ","doi":"10.1021/acsmeasuresciau.3c00002","DOIUrl":"10.1021/acsmeasuresciau.3c00002","url":null,"abstract":"Fluorescence-based single-molecule approaches have helped revolutionize our understanding of chemical and biological mechanisms. Unfortunately, these methods are only suitable at low concentrations of fluorescent molecules so that single fluorescent species of interest can be successfully resolved beyond background signal. The application of these techniques has therefore been limited to high-affinity interactions despite most biological and chemical processes occurring at much higher reactant concentrations. Fortunately, recent methodological advances have demonstrated that this concentration barrier can indeed be broken, with techniques reaching concentrations as high as 1 mM. The goal of this Review is to discuss the challenges in performing single-molecule fluorescence techniques at high-concentration, offer applications in both biology and chemistry, and highlight the major milestones that shatter the concentration barrier. We also hope to inspire the widespread use of these techniques so we can begin exploring the new physical phenomena lying beyond this barrier.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 4","pages":"239–257"},"PeriodicalIF":0.0,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/43/tg3c00002.PMC10436376.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Correction to “Why and How Savitzky–Golay Filters Should Be Replaced” 更正“为什么以及如何更换Savitzky-Golay过滤器”

ACS Measurement Science Au Pub Date : 2023-05-04 DOI: 10.1021/acsmeasuresciau.3c00017

Michael Schmid*, David Rath and Ulrike Diebold,

引用次数: 0

Calculation of Tissue Oxygenation via an Inverse Boundary Problem for Transcutaneous Oxygenation Wearable Applications 基于反边界问题的经皮氧合可穿戴应用组织氧合计算

ACS Measurement Science Au Pub Date : 2023-05-02 DOI: 10.1021/acsmeasuresciau.3c00013

Juan Pedro Cascales*, Adina E. Draghici, Helen Keshishian, J. Andrew Taylor and Conor L. Evans*,

引用次数: 1

Multiplex Assays Enable Simultaneous Detection and Identification of SARS-CoV-2 Variants of Concern in Clinical and Wastewater Samples 多重检测能够同时检测和鉴定临床和废水样本中关注的SARS-CoV-2变体

ACS Measurement Science Au Pub Date : 2023-04-21 DOI: 10.1021/acsmeasuresciau.3c00005

Yanming Liu, Teresa Kumblathan, Michael A. Joyce, D. Lorne Tyrrell, Graham Tipples, Xiaoli Pang, Xing-Fang Li* and X. Chris Le*,

{"title":"Multiplex Assays Enable Simultaneous Detection and Identification of SARS-CoV-2 Variants of Concern in Clinical and Wastewater Samples","authors":"Yanming Liu, Teresa Kumblathan, Michael A. Joyce, D. Lorne Tyrrell, Graham Tipples, Xiaoli Pang, Xing-Fang Li* and X. Chris Le*, ","doi":"10.1021/acsmeasuresciau.3c00005","DOIUrl":"10.1021/acsmeasuresciau.3c00005","url":null,"abstract":"The targeted screening and sequencing approaches for COVID-19 surveillance need to be adjusted to fit the evolving surveillance objectives which necessarily change over time. We present the development of variant screening assays that can be applied to new targets in a timely manner and enable multiplexing of targets for efficient implementation in the laboratory. By targeting the HV69/70 deletion for Alpha, K417N for Beta, K417T for Gamma, and HV69/70 deletion plus K417N for sub-variants BA.1, BA.3, BA.4, and BA.5 of Omicron, we achieved simultaneous detection and differentiation of Alpha, Beta, Gamma, and Omicron in a single assay. Targeting both T478K and P681R mutations enabled specific detection of the Delta variant. The multiplex assays used in combination, targeting K417N and T478K, specifically detected the Omicron sub-variant BA.2. The limits of detection for the five variants of concern were 4–16 copies of the viral RNA per reaction. Both assays achieved 100% clinical sensitivity and 100% specificity. Analyses of 377 clinical samples and 24 wastewater samples revealed the Delta variant in 100 clinical samples (nasopharyngeal and throat swab) collected in November 2021. Omicron BA.1 was detected in 79 nasopharyngeal swab samples collected in January 2022. Alpha, Beta, and Gamma variants were detected in 24 wastewater samples collected in May–June 2021 from two major cities of Alberta (Canada), and the results were consistent with the clinical cases of multiple variants reported in the community.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 4","pages":"258–268"},"PeriodicalIF":0.0,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/dc/tg3c00005.PMC10152402.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Electrochemical Cellulase-Linked ELASA for Rapid Liquid Biopsy Testing of Serum HER-2/neu 电化学纤维素酶连接的ELASA快速液体活检检测血清HER-2/neu

ACS Measurement Science Au Pub Date : 2023-04-14 DOI: 10.1021/acsmeasuresciau.2c00067

Ana Díaz-Fernández, Alexey Ferapontov, Mikkel Holm Vendelbo and Elena E. Ferapontova*,

{"title":"Electrochemical Cellulase-Linked ELASA for Rapid Liquid Biopsy Testing of Serum HER-2/neu","authors":"Ana Díaz-Fernández, Alexey Ferapontov, Mikkel Holm Vendelbo and Elena E. Ferapontova*, ","doi":"10.1021/acsmeasuresciau.2c00067","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.2c00067","url":null,"abstract":"Non-invasive liquid biopsy assays for blood-circulating biomarkers of cancer allow both its early diagnosis and treatment monitoring. Here, we assessed serum levels of protein HER-2/neu, overexpressed in a number of aggressive cancers, by the cellulase-linked sandwich bioassay on magnetic beads. Instead of traditional antibodies we used inexpensive reporter and capture aptamer sequences, transforming the enzyme-linked immuno-sorbent assay (ELISA) into an enzyme-linked aptamer-sorbent assay (ELASA). The reporter aptamer was conjugated to cellulase, whose digestion of nitrocellulose film electrodes resulted in the electrochemical signal change. ELASA, optimized relative aptamer lengths (dimer vs monomer and trimer), and assay steps allowed 0.1 fM detection of HER-2/neu in the 10% human serum in 1.3 h. Urokinase plasminogen activator and thrombin as well as human serum albumin did not interfere, and liquid biopsy analysis of serum HER-2/neu was similarly robust but 4 times faster and 300 times cheaper than both electrochemical and optical ELISA. Simplicity and low cost of cellulase-linked ELASA makes it a perspective diagnostic tool for fast and accurate liquid biopsy detection of HER-2/neu and of other proteins for which aptamers are available.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 3","pages":"226–235"},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.2c00067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49767905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Novel Additive Manufactured Multielectrode Electrochemical Cell with Honeycomb Inspired Design for the Detection of Methyl Parathion in Honey Samples 新型添加剂制造的蜂窝设计多电极电化学电池用于蜂蜜样品中甲基对硫磷的检测

ACS Measurement Science Au Pub Date : 2023-04-06 DOI: 10.1021/acsmeasuresciau.3c00003

Bruno C. Janegitz*, Robert D. Crapnell, Paulo Roberto de Oliveira, Cristiane Kalinke, Matthew J. Whittingham, Alejandro Garcia-Miranda Ferrari and Craig E. Banks*,

{"title":"Novel Additive Manufactured Multielectrode Electrochemical Cell with Honeycomb Inspired Design for the Detection of Methyl Parathion in Honey Samples","authors":"Bruno C. Janegitz*, Robert D. Crapnell, Paulo Roberto de Oliveira, Cristiane Kalinke, Matthew J. Whittingham, Alejandro Garcia-Miranda Ferrari and Craig E. Banks*, ","doi":"10.1021/acsmeasuresciau.3c00003","DOIUrl":"10.1021/acsmeasuresciau.3c00003","url":null,"abstract":"The development and increase in the number of crops recently have led to the requirement for greater efficiency in world food production and greater consumption of pesticides. In this context, the widespread use of pesticides has affected the decrease in the population of pollinating insects and has caused food contamination. Therefore, simple, low-cost, and quick analytical methods can be interesting alternatives for checking the quality of foods such as honey. In this work, we propose a new additively manufactured (3D-printed) device inspired by a honeycomb cell, with 6 working electrodes for the direct electrochemical analysis of methyl parathion by reduction process monitoring in food and environmental samples. Under optimized parameters, the proposed sensor presented a linear range between 0.85 and 19.6 μmol L–1, with a limit of detection of 0.20 μmol L–1. The sensors were successfully applied in honey and tap water samples by using the standard addition method. The proposed honeycomb cell made of polylactic acid and commercial conductive filament is easy to construct, and there is no need for chemical treatments to be used. These devices based on 6 working electrodes array are versatile platforms for rapid, highly repeatable analysis in food and environment, capable of performing detection in low concentrations.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 3","pages":"217–225"},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Electrochemical Detection of Borrelia burgdorferi Using a Biomimetic Flow Cell System 基于仿生流动池系统的伯氏疏螺旋体电化学检测

ACS Measurement Science Au Pub Date : 2023-03-30 DOI: 10.1021/acsmeasuresciau.3c00004

Connor D. Flynn*, Mariusz Sandomierski, Kelly Kim, Julie Lewis, Vett Lloyd and Anna Ignaszak*,

{"title":"Electrochemical Detection of Borrelia burgdorferi Using a Biomimetic Flow Cell System","authors":"Connor D. Flynn*, Mariusz Sandomierski, Kelly Kim, Julie Lewis, Vett Lloyd and Anna Ignaszak*, ","doi":"10.1021/acsmeasuresciau.3c00004","DOIUrl":"https://doi.org/10.1021/acsmeasuresciau.3c00004","url":null,"abstract":"Lyme disease, caused by infection with pathogenic Borrelia bacteria, has emerged as a pervasive illness throughout North America and many other regions of the world in recent years, owing in part to climate-mediated habitat expansion of the tick vectors. Standard diagnostic testing has remained largely unchanged over the past several decades and is indirect, relying on detection of antibodies against the Borrelia pathogen, rather than detection of the pathogen itself. The development of new rapid, point-of-care tests for Lyme disease that directly detects the pathogen could drastically improve patient health by enabling faster and more frequent testing that could better inform patient treatment. Here, we describe a proof-of-concept electrochemical sensing approach to the detection of the Lyme disease-causing bacteria, which utilizes a biomimetic electrode to interact with the Borrelia bacteria that induce impedance alterations. In addition, the catch-bond mechanism between bacterial BBK32 protein and human fibronectin protein, which exhibits improved bond strength with increased tensile force, is tested within an electrochemical injection flow-cell to achieve Borrelia detection under shear stress.","PeriodicalId":29800,"journal":{"name":"ACS Measurement Science Au","volume":"3 3","pages":"208–216"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmeasuresciau.3c00004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

New Guidelines for Presenting Electrochemical Data in All ACS Journals 在所有ACS期刊上发表电化学数据的新指南

ACS Measurement Science Au Pub Date : 2023-03-20 DOI: 10.1021/acsmeasuresciau.3c00008

Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant V. Kamat, Marisa Kozlowski, Géraldine Masson and Scott J. Miller,

引用次数: 0