Cytokine最新文献

{"title":"Immune microenvironment associated with the severity of Langerhans cell histiocytosis in children","authors":"Fengqing Cai ,&nbsp;Zhaoyang Peng ,&nbsp;Hui Xu ,&nbsp;Hui Gao ,&nbsp;Chan Liao ,&nbsp;Xiaojun Xu ,&nbsp;Xiaoping Guo ,&nbsp;Weizhong Gu ,&nbsp;Kun Zhu ,&nbsp;Qiang Shu ,&nbsp;Hongqiang Shen","doi":"10.1016/j.cyto.2023.156378","DOIUrl":"10.1016/j.cyto.2023.156378","url":null,"abstract":"<div><p>The aim of this study is to investigate the clinical potential of immune microenvironment in peripheral blood for the severity and therapeutic efficacy of Langerhans cell histiocytosis (LCH). A total of 200 newly diagnosed children with LCH during 10 years was enrolled for analysis in this study. Peripheral blood samples were acquired from patients before treatment in our hospital and immune indicators were detected by a four-color flow cytometer. The levels of CD3 + CD8 + T cell, CD3 + CD4 + HLA-DR + T cell, CD3 + CD8 + HLA-DR + T cell, IL-4, IL-6, IL-10 and IFN-γ in peripheral blood were markedly elevated in LCH patients vs. healthy controls. Patients with multiple system with risk organ involvement (MS-RO + ) exhibited higher levels in IL-6, IL-10 and IFN-γ, CD3 + CD4 + HLA-DR + T cell and CD3 + CD8 + HLA-DR + T cell, compared to those in patients without risk organ involvement (RO-). Patients who responded effectively to initial chemotherapy showed significantly lower levels of IL-4, IL-10, IFN-γ, CD3 + CD4 + HLA-DR + T cell and CD3 + CD8 + HLA-DR + T cell in peripheral blood, compared to those in patients who did not respond to initial chemotherapy. Furthermore, univariate analyses were performed that the higher levels of CD3 + CD4 + HLA-DR + T cells, CD3 + CD8 + HLA-DR + T cells and IL-10 in peripheral blood were related to non-response in LCH after initial chemotherapy. Immune microenvironment in peripheral blood may be associated with the severity and treatment response of LCH. The levels of CD3 + CD4 + HLA-DR + T cells, CD3 + CD8 + HLA-DR + T cells and IL-10 may be biomarkers to predict treatment response of LCH patients.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156378"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1043466623002569/pdfft?md5=aeea72b9e90ab4e7d6fe330c60757256&pid=1-s2.0-S1043466623002569-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41096005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene signature based on glycolysis is closely related to immune infiltration of patients with osteoarthritis","authors":"Ziyi Chen,&nbsp;Yinghui Hua","doi":"10.1016/j.cyto.2023.156377","DOIUrl":"10.1016/j.cyto.2023.156377","url":null,"abstract":"<div><h3>Background</h3><p>Osteoarthritis (OA) is a degenerative arthritis with high levels of clinical heterogeneity. Aberrant metabolism such as shifting from oxidative phosphorylation to glycolysis is a response to changes in the inflammatory microenvironment of OA. Therefore, there is a pressing need to identify novel glycolysis regulators during OA progression.</p></div><div><h3>Methods</h3><p>We systematically studied glycolysis patterns mediated by 141 glycolysis regulators in 74 human synovial samples and discussed the characteristics of the immune microenvironment modified by glycolysis. The random forest (RF) method was applied to screen candidate hub glycolysis regulators in OA. RT-qPCR was performed to validate these key regulators. Then distinct glycolysis patterns were identified, and systematic correlation between these glycolysis patterns and immune cell infiltration was analyzed. The glycolysis score was constructed to quantify glycolysis patterns together with immune infiltration of individual OA patient.</p></div><div><h3>Results</h3><p>56 glycolysis-related differentially expressed genes (DEGs) were identified between OA and non-OA samples. <em>STC1, VEGFA, KDELR3, DDIT4</em> and <em>PGAM1</em> were selected as candidate genes to predict the probability of OA. Two glycolysis patterns in OA were identified. Glycolysis cluster A with higher glycolysis score was related to an inflamed phenotype.</p></div><div><h3>Conclusions</h3><p>Taken together, our results established a glycolysis-based genetic signature for OA, guided in-depth studies on the metabolic mechanism of OA, and facilitated to explore new clinical treatment strategies.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156377"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41100830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the complex role of interferon-gamma in suppressing type 2 immunity to cancer","authors":"Asif Ahmad Bhat ,&nbsp;Ahsas Goyal ,&nbsp;Riya Thapa ,&nbsp;Waleed Hassan almalki ,&nbsp;Imran Kazmi ,&nbsp;Sami I. Alzarea ,&nbsp;Mahaveer Singh ,&nbsp;Suman Rohilla ,&nbsp;Tarun Kumar Saini ,&nbsp;Neelima Kukreti ,&nbsp;Dhanalekshmi Unnikrishnan Meenakshi ,&nbsp;Neeraj Kumar Fuloria ,&nbsp;Mahendran Sekar ,&nbsp;Gaurav Gupta","doi":"10.1016/j.cyto.2023.156376","DOIUrl":"10.1016/j.cyto.2023.156376","url":null,"abstract":"<div><p>Cancer involves cells' abnormal growth and ability to invade or metastasize to different body parts. Cancerous cells can divide uncontrollably and spread to other areas through the lymphatic or circulatory systems. Tumors form when malignant cells clump together in an uncontrolled manner. In this context, the cytokine interferon-gamma (IFN-γ) is crucial in regulating immunological responses, particularly malignancy. While IFN-γ is well-known for its potent anti-tumor effects by activating type 1 immunity, recent research has revealed its ability to suppress type 2 immunity, associated with allergy and inflammatory responses. This review aims to elucidate the intricate function of IFN-γ in inhibiting type 2 immune responses to cancer. We explore how IFN-γ influences the development and function of immune cells involved in type 2 immunity, such as mast cells, eosinophils, and T-helper 2 (Th2) cells. Additionally, we investigate the impact of IFN-mediated reduction of type 2 immunity on tumor development, metastasis, and the response to immunotherapeutic interventions. To develop successful cancer immunotherapies, it is crucial to comprehend the complex interplay between type 2 and type 1 immune response and the regulatory role of IFN-γ. This understanding holds tremendous promise for the development of innovative treatment approaches that harness the abilities of both immune response types to combat cancer. However, unraveling the intricate interplay between IFN-γ and type 2 immunity in the tumor microenvironment will be essential for achieving this goal.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156376"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41090812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcipotriol suppresses GPX4-mediated ferroptosis in OA chondrocytes by blocking the TGF-β1 pathway","authors":"Zhicheng Yang ,&nbsp;Wei Jiang ,&nbsp;Chenwei Xiong ,&nbsp;JingJing Shang ,&nbsp;Yong Huang ,&nbsp;Xindie Zhou ,&nbsp;Su Zhang","doi":"10.1016/j.cyto.2023.156382","DOIUrl":"10.1016/j.cyto.2023.156382","url":null,"abstract":"<div><p>Globally, tens of millions of individuals experience osteoarthritis (OA), a degenerative joint condition for which a definitive cure is currently lacking. This condition is characterized by joint inflammation and the progressive deterioration of articular cartilage. In this study, western blotting, quantitative reverse-transcription polymerase chain reaction, and immunofluorescence analysis were performed to elucidate the molecular mechanisms by which calcipotriol alleviates chondrocyte ferroptosis. The effect of calcipotriol on reactive oxygen species and lipid peroxidation levels in chondrocytes was assessed using dihydroethidium staining and the fluorescent dye BODIPY. To replicate OA, the destabilized medial meniscus model was employed, followed by the injection of calcipotriol into the knee articular cavity. Morphological analysis was conducted through hematoxylin and eosin staining, safranin O-Fast green staining, and micro-computed tomography analysis. Immunohistochemical analysis was performed to validate the effect of calcipotriol in vivo. Our results demonstrate that the expression of SOX9, col2a1, and Aggrecan, as well as MMP13 and ADAMTS5 protein expression levels, decrease upon treatment with calcipotriol in interleukin-1β stimulated chondrocytes. Despite these promising outcomes, the exact mechanism underlying calcipotriol's therapeutic effect on OA remains uncertain. We discovered that calcipotriol inhibits chondrocyte GPX4-mediated ferroptosis by suppressing the expression of transforming growth factor-β1. Furthermore, our study established an in vivo model of OA using rats with medial meniscus instability. Our experiments on rats with OA revealed that intra-articular calcipotriol injection significantly reduces cartilage degradation caused by the disease. Our findings suggest that calcipotriol can mitigate OA by impeding GPX4-mediated ferroptosis of chondrocytes, achieved through the suppression of the TGF-β1 pathway.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156382"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41094286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cord blood granulocyte Colony-Stimulating factor level as an optimal predictor of umbilical cord arteritis associated with brain injury at term equivalent age in preterm neonates","authors":"Jun Nirei ,&nbsp;Akira Kobayashi ,&nbsp;Rie Habuka ,&nbsp;Hisanori Domon ,&nbsp;Yutaka Terao ,&nbsp;Akihiko Saitoh","doi":"10.1016/j.cyto.2023.156369","DOIUrl":"10.1016/j.cyto.2023.156369","url":null,"abstract":"<div><h3>Objective</h3><p>The study aimed 1) to evaluate the association between the presence or absence of umbilical cord arteritis (UCA) and the cord blood cytokine levels, and 2) morbidity and mortality of preterm neonates; and 3) to identify predictive markers for UCA of preterm neonates.</p></div><div><h3>Study design</h3><p>In this single-center retrospective observational cohort study, preterm neonates born at gestational age (GA) &lt; 36 weeks were categorized pathologically according to the severity of intrauterine inflammation; those without UCA as Group 1, those with UCA as Group 2, and those without any intrauterine inflammation as Group 3 (control), and subgroup analyses classified by their GA were performed. We compared morbidity and mortality, and eight representative cytokine levels in cord blood samples between the groups. Subsequently, receiver operating characteristics (ROC) curves for UCA diagnosis for each cytokine were created, and values of areas under the curve (AUC) were calculated to determine the optimal predictive markers.</p></div><div><h3>Results</h3><p>In total, 105 patients (36, 58, and 11 in Groups 1, 2, and 3, respectively) were included. Multivariate logistic analysis revealed that patients with UCA had higher incidence of brain injury (Odds Ratio [OR] = 8.53, P = 0.0049, 95% Confidence Interval [CI]: 1.91 – 38.0), at term equivalent age in the subgroup analysis with GA &lt; 32 weeks. Although the median value of cord blood granulocyte colony-stimulating factor (G-CSF) was significantly higher in Group 2 than in Group 1 or 3, only the G-CSF level was found to be high in the subgroup analysis with GA &lt; 32 weeks. For UCA diagnosis, the AUC values of G-CSF were the highest among eight cytokines including interleukin 6 (IL-6). These findings were similar in the subgroup analysis with GA &lt; 32 weeks.</p></div><div><h3>Conclusions</h3><p>Preterm neonates, especially born at GA &lt; 32 week, had higher morbidity from　brain injury in the group with UCA. The cord blood G-CSF level was highly accurate for predicting UCA and could thus be used as an optimal biomarker.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156369"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41098596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of Interleukin-36s in cancers: A systematic review and meta-analysis","authors":"Rui Zhang ,&nbsp;Mengyuan Jiang ,&nbsp;Min Huang ,&nbsp;Jing Yang ,&nbsp;Qianqian Liu ,&nbsp;Ziru Zhao ,&nbsp;Yuping Bai ,&nbsp;Tingting He ,&nbsp;Dengcai Zhang ,&nbsp;Min Zhang","doi":"10.1016/j.cyto.2023.156397","DOIUrl":"10.1016/j.cyto.2023.156397","url":null,"abstract":"<div><h3>Background</h3><p>Interleukin-36s (IL-36s) are a category of inflammatory cytokines and an increasing number of studies over the past decade have found that different kinds of IL-36s play different roles in cancers. This systematic review and <em>meta</em>-analysis aimed to evaluate the prognostic value of IL-36s in different cancer types.</p></div><div><h3>Method</h3><p>Two reviewers independently searched in PubMed, Cochrane Library and EMBASE up to December 13, 2022. We extracted the hazard ratio (HR) and the confidence intervals (CIs) of the related prognostic outcomes and analyzed the pooled HR.</p></div><div><h3>Results</h3><p>We included 12 studies including 1925 patients. In all, six studies including IL-36α, five including IL-36γ and one including IL-36β. A high expression of IL-36α was associated with better overall survival (OS) (HR = 0.48, 95 %CI: 0.37–0.62, <em>P</em> &lt; 0.001) of cancer patients. The expression of IL-36γ was not related with cancers. Further, subgroup analysis showed that the expression of IL-36γ had no correlation with the OS of colorectal cancer (CRC) and non‑small cell lung cancer (NSCLC) patients. Interestingly, a high expression of IL-36γ played contrasting prognostic roles in hepatocellular carcinoma (HCC) (HR = 0.43, 95 %CI: 0.27–0.69, <em>P</em> &lt; 0.001) patients and gastric cancer (GC) (HR = 1.58, 95 %CI: 1.33–1.87, <em>P</em> &lt; 0.001) patients. The only IL-36β related study showed the expression of IL-36β was not correlated with the prognosis of CRC patients (<em>P</em> &gt; 0.05).</p></div><div><h3>Conclusion</h3><p>IL-36α, IL-36β and IL-36γ possibly play different roles in different cancers. High expression of IL-36α may be associated with good prognostic value in cancer patients, especially in CRC patients. The association between cancers prognosis and expression of IL-36β or IL-36γ needs further evaluation. These conclusions need more clinical prognostic data for confirmation.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"172 ","pages":"Article 156397"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1043466623002752/pdfft?md5=1f2c25d5d1e8b4e0a4f07f3818c1a84f&pid=1-s2.0-S1043466623002752-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71476152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin protects against Aspergillus fumigatus keratitis by reducing fungal load and inhibiting TLR-4 induced inflammatory response","authors":"Junjie Luan ,&nbsp;Yunan Zhu ,&nbsp;Jing Lin ,&nbsp;Yingxue Zhang ,&nbsp;Qiang Xu ,&nbsp;Lu Zhan ,&nbsp;Xue Tian ,&nbsp;Guiqiu Zhao ,&nbsp;Xudong Peng","doi":"10.1016/j.cyto.2023.156356","DOIUrl":"10.1016/j.cyto.2023.156356","url":null,"abstract":"<div><h3>Purpose</h3><p>To investigate the antifungal and anti-inflammatory effects of quercetin in <em>Aspergillus fumigatus</em> (<em>A. fumigatus</em>) keratitis.</p></div><div><h3>Methods</h3><p>Draize eye test was performed in mice to evaluate the toxicity of quercetin, and the antifungal effects on <em>A. fumigatus</em> were assessed via scanning electron microscopy (SEM), propidium iodide uptake, and adherence assay. In fungal keratitis (FK) mouse models, immunostaining was performed for investigating toll-like receptor 4 (TLR-4) expression and macrophage infiltration. Real-time PCR, ELISA, and Western blot were used to evaluate the expression of pro-inflammatory factors IL-1β, TNF-α, and IL-6 in infected RAW264.7 cells. Cells were also treated with TLR-4 siRNA or agonist CRX-527 to investigate mechanisms underlying the anti-inflammatory activity of quercetin.</p></div><div><h3>Results</h3><p>Quercetin at 32 μM was non-toxic to corneal epithelial and significantly inhibited <em>A. fumigatus</em> growth and adhesion, and also altered the structure and reduced the number of mycelia. Quercetin significantly reduced macrophage infiltration in the mouse cornea, and attenuated the expression of TLR-4 in the corneal epithelium and stroma of mice with keratitis caused by <em>A. fumigatus</em>. In RAW264.7 cells infected by <em>A. fumigatus</em>, quercetin downregulated TLR-4 along with pro-inflammatory factors IL-1β, TNF-α, and IL-6. RAW cells with TLR-4 knockdown had reduced expression of factors after <em>A. fumigatus</em> infection, which was decreased even further with quercetin treatment. In contrast, cells with CRX-527 had elevated inflammatory factors compared to control, which was significantly attenuated in the presence of quercetin.</p></div><div><h3>Conclusion</h3><p>Quercetin plays a protective role in mouse <em>A. fumigatus</em> keratitis by inhibiting fungal load, disrupting hyphae structure, macrophage infiltration, and suppressing inflammation response in macrophages via TLR-4 mediated signaling pathway.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156356"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10237825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity","authors":"Julia Lischka: ,&nbsp;Andrea Schanzer ,&nbsp;Charlotte de Gier ,&nbsp;Susanne Greber-Platzer ,&nbsp;Maximilian Zeyda","doi":"10.1016/j.cyto.2023.156372","DOIUrl":"10.1016/j.cyto.2023.156372","url":null,"abstract":"<div><h3>Backgpound</h3><p>Metabolically driven chronic low-grade adipose tissue inflammation, so-called metaflammation, is a central feature in obesity. This inflammatory tone is largely driven by adipose tissue macrophages (ATM), which express pro- and anti-inflammatory markers and cytokines such as, e.g., IL-1 receptor antagonist (IL-1RA), CD163 and osteopontin (OPN). Metaflammation ultimately leads to the development of cardiometabolic diseases. This study aimed to evaluate the association between selected adipose tissue macrophage-associated markers and metabolic comorbidities in pediatric obesity.</p></div><div><h3>Methods</h3><p>From a pediatric cohort with obesity (n = 108), clinically thoroughly characterized including diverse routine blood parameters, oral glucose tolerance test and liver MRI, plasma IL-1RA, soluble (s)CD163 and OPN were measured by ELISA.</p></div><div><h3>Results</h3><p>We observed significantly higher IL-1RA, sCD163, and OPN levels in the plasma of children with metabolic-dysfunction associated fatty liver disease (MAFLD) and metabolic syndrome. Moreover, IL-1RA and sCD163 correlated with hepatic disease and apoptosis markers alanine aminotransferase and CK-18. IL-1RA concentrations additionally correlated with insulin resistance, while children with disturbed glucose metabolism had significantly higher levels of sCD163.</p></div><div><h3>Conclusion</h3><p>MAFLD and other metabolic disorders in pediatric patients with obesity are associated with an elevation of adipose tissue macrophage-related inflammation markers.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156372"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1043466623002508/pdfft?md5=01b185697590279193553157a3aad364&pid=1-s2.0-S1043466623002508-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41090811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of inflammation markers in human blood collected using volumetric absorptive microsampling (VAMS) under typical laboratory storage temperatures","authors":"R. McMahon ,&nbsp;C. Hill ,&nbsp;J. Rudge ,&nbsp;B. Herbert ,&nbsp;E. Karsten","doi":"10.1016/j.cyto.2023.156355","DOIUrl":"10.1016/j.cyto.2023.156355","url":null,"abstract":"<div><p>Dried blood spots (DBS) collected on filter paper such as Guthrie cards are stored for years at room temperature. The assumption is that once dried, the samples remain stable and quantifiable indefinitely since the metabolites these were initially designed to measure, are known for their extended stability. The concentration of other blood proteins such as cytokines, however, are known to vary with storage even in liquid samples stored at −80 °C for extended periods of time. We sought to determine if cytokines are stable for up to 5 months when stored as a dried blood sample using volumetric absorptive microsampling (VAMS) devices.</p><p>To test this, blood was collected from 4 healthy participants, spiked with recombinant cytokines, and collected into 30 µL VAMS devices. These prepared VAMS devices were stored at room temperature, 4 °C, or −20 °C for up to 5 months and matching VAMS liquid extracts were stored at −80 °C for the same period of time. At each timepoint, the samples were extracted from the VAMS devices and the extracts were analysed by Luminex® for quantification of up to 31 cytokines. These methods were also tested in a remote clinical study over a period of up to 8 months.</p><p>Cytokine analysis revealed that room temperature, the current standard for DBS and VAMS storage, performed the poorest out of all storage temperatures with significant losses in 13/21 analytes compared to 4 °C at 5 months. Storage at 4 °C or colder performed well for the majority of analytes tested, however out of those, the optimal storage temperature differed for each analyte. There were a small number of analytes that performed poorly regardless of storage conditions and for fractalkine, this was found to be caused by inefficient recovery during extraction. Cytokine concentrations from finger-prick samples were also found to be much more variable that those in venous blood samples.</p><p>Our results highlight the need to understand the stability of analytes of interest before committing to longitudinal collection and storage of samples in VAMS devices. These data give confidence that storage at 4 °C or colder was beneficial for cytokine stability. Wherein 25/31 cytokines were quantifiably stable at −20 °C when stored for 3 months and 17/21 were quantifiably stable after 5 months when stored at 4 °C.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156355"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1043466623002338/pdfft?md5=075c5f045fd949c2fc17d0af87f4c7e0&pid=1-s2.0-S1043466623002338-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10204895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential role of interleukins and interferons in ovarian cancer","authors":"Huldani Huldani ,&nbsp;Sana Abdul-Jabbar Ali ,&nbsp;F. Al-dolaimy ,&nbsp;Ahmed Hjazi ,&nbsp;Nikolenko Denis Andreevich ,&nbsp;Khulood H. Oudaha ,&nbsp;Abbas F. Almulla ,&nbsp;Ali Alsaalamy ,&nbsp;Shamam Kareem Oudah ,&nbsp;Yasser Fakri Mustafa","doi":"10.1016/j.cyto.2023.156379","DOIUrl":"10.1016/j.cyto.2023.156379","url":null,"abstract":"<div><p>Ovarian cancer poses significant challenges and remains a highly lethal disease with limited treatment options. In the context of ovarian cancer, interleukins (ILs) and interferons (IFNs), important cytokines that play crucial roles in regulating the immune system, have emerged as significant factors influencing its development. This article provides a comprehensive review of the involvement of various ILs, including those from the IL-1 family, IL-2 family, IL-6 family, IL-8 family, IL-10 family, and IL-17 family, in ovarian cancer. The focus is on their impact on tumor growth, metastasis, and their role in evading immune responses within the tumor microenvironment. Additionally, the article conducts an in-depth examination of the oncogenic or antitumor roles of each IL in the context of ovarian cancer pathogenesis and progression. Besides, we elucidated the enhancements in the treatment of ovarian cancer through the utilization of type-I IFN and type-II IFN. Recent research has shed light on the intricate mechanisms through which specific ILs and IFNs contribute to the advancement of the disease. By incorporating recent findings, this review also seeks to inspire further investigations into unexplored mechanisms, fostering ongoing research to develop more effective therapeutic strategies for ovarian cancer. Moreover, through an in-depth analysis of IL- and IFN-associated clinical trials, we have highlighted their promising potential of in the treatment of ovarian cancer. These clinical trials serve to reinforce the significant outlook for utilizing ILs and IFNs as therapeutic agents in combating this disease.</p></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"171 ","pages":"Article 156379"},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41101491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}