Cytokine最新文献

{"title":"Pro-inflammatory regulation of EGFR-Annexin A2 synergy by sodium butyrate: An upstream target to reduce articular inflammation and cartilage destruction in rheumatoid arthritis","authors":"Pavan K. Jayaswamy ,&nbsp;Vikram Haridas ,&nbsp;M. Vijaykrishnaraj ,&nbsp;Vinay C. Sangamesh ,&nbsp;Thrupthi Ambrish ,&nbsp;Jayaprakash Shetty ,&nbsp;Adithi Kellarai ,&nbsp;Shashi Kumar Shetty ,&nbsp;Prakash Patil ,&nbsp;Praveenkumar Shetty","doi":"10.1016/j.cyto.2025.157008","DOIUrl":"10.1016/j.cyto.2025.157008","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a debilitating autoimmune disease characterized by persistent articular inflammation and joint damage. Our prior research identified a pro-inflammatory interplay between the epidermal growth factor receptor (EGFR) and Annexin A2 (AnxA2), which is exacerbated by Annexin A1 (AnxA1) downregulation in RA pathogenesis. The present study elucidates the immunomodulatory potential of sodium butyrate, a gut microbiota-derived short-chain fatty acid, in disrupting the EGFR-AnxA2 axis and restoring AnxA1 homeostasis. Employing an integrative in vitro–in vivo paradigm, we establish that sodium butyrate attenuates EGFR and AnxA2 expression in primary human RA synovial fibroblasts, concomitantly upregulating AnxA1 and diminishing TNF-α release. In vivo, using a collagen-induced arthritis (CIA) murine model, histopathological and molecular analysis reveal that sodium butyrate-treated mice exhibit significantly attenuated synovial hyperplasia, reduced pannus formation, and elevated AnxA1 expression, culminating in a disruption of EGFR-AnxA2 crosstalk. Furthermore, X-ray computed tomography substantiates a marked preservation of joint architecture, with diminished osteolytic lesions and joint space narrowing, notably in the tibia and calcaneum. These findings underscore sodium butyrate's potential as regulator of EGFR-AnxA2 complex modulator that reinstates anti-inflammatory homeostasis through AnxA1 induction, attenuating RA-mediated inflammatory cascades and structural deterioration.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157008"},"PeriodicalIF":3.7,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144805825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cytokine TGF-β1 on pathological changes in diabetic retinopathy","authors":"Ilona Hartmane ,&nbsp;Ingmars Mikazans","doi":"10.1016/j.cyto.2025.157004","DOIUrl":"10.1016/j.cyto.2025.157004","url":null,"abstract":"<div><div>The purpose of the study was to determine the role of the cytokine transforming growth factor beta 1 (TGF-β1) in the progression of diabetic retinopathy and to assess its importance as a potential therapeutic target for slowing down pathological changes in the retina. To achieve this goal, scientific publications selected from leading international databases were analysed. The studies investigated the molecular mechanisms of TGF-β1 activation, including Smad-dependent and Smad-independent pathways, such as MAPK, PI3K/Akt, Wnt/β-catenin and NF-κB, which play a key role in fibrotic, angiogenic and inflammatory processes. Particular attention was paid to the interaction of TGF-β1 with vascular endothelial growth factor (VEGF), which promotes the formation of new pathological vessels with increased permeability, as well as the regulation of the extracellular matrix. The analysis revealed that TGF-β1 is a key regulator of structural changes in the retina under hyperglycaemia, affecting basement membrane thickening, disruption of the blood-retinal barrier, increased vascular permeability and the development of chronic inflammation. It has been shown that elevated levels of TGF-β1 contribute to the progression of fibrosis by activating the epithelial-mesenchymal transition of retinal pigment epithelial cells, which leads to tissue remodelling. In addition, it was found that activation of TGF-β1 under the influence of hyperglycaemia is associated with increased oxidative stress, inflammatory reactions and angiogenesis. The analysis of experimental studies in animal models where streptozotocin was used to induce hyperglycaemia, as well as clinical observations of patients with diabetic retinopathy, allowed to assess the effectiveness of TGF-β1 inhibitors in reducing fibrotic changes, angiogenesis, and chronic inflammation. The results obtained contribute to the formation of a theoretical basis for the further development of therapeutic strategies aimed at inhibiting TGF-β1 as an important component of the pathogenesis of diabetic retinopathy.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated cytokine levels in patients with High-altitude pulmonary edema","authors":"Binyun Liu ,&nbsp;Quzong Zhaxi ,&nbsp;Zhuoga Danzeng ,&nbsp;Bai Ci ,&nbsp;Qiangba Dingzeng ,&nbsp;Zhuoga Baima ,&nbsp;Luobu Gesang","doi":"10.1016/j.cyto.2025.157005","DOIUrl":"10.1016/j.cyto.2025.157005","url":null,"abstract":"<div><h3>Background</h3><div>Immunomodulation is integral to the body's adaptation to varying altitudes. Nevertheless, the effects of immune regulation on the onset of high-altitude pulmonary edema (HAPE) are not well understood. This research aimed to explore the influence of immune regulation on HAPE pathogenesis through the assessment of cytokine levels.</div></div><div><h3>Methods</h3><div>We analyzed the cytokine profiles of 28 HAPE patients at high altitudes and compared them to 25 healthy individuals who had successfully acclimatized. The levels of seven cytokines released by T helper cells (Th)1/2/17, alongside monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-8, and IL-1β in serum, were quantified using cytometric bead array (CBA) technology.</div></div><div><h3>Results</h3><div>Our findings revealed significantly higher concentrations of IL-2, IL-10, and tumor necrosis factor (TNF) in the peripheral blood of HAPE patients when contrasted with those of healthy individuals (<em>P</em> &lt; 0.001). A comprehensive analysis of these cytokines indicated a robust diagnostic capability for predicting HAPE, achieving an area under the curve (AUC) of 0.98. Conversely, no significant differences were observed in the levels of IL-6, IL-8, interferon-γ (IFN-γ), IL-4, IL-17 A, MCP-1, and IL-1β between the two cohorts.</div></div><div><h3>Conclusions</h3><div>Elevated IL-2, IL-10, and TNF in HAPE patients underscore immune dysregulation as a disease driver. Clinically, these cytokines may guide risk prediction (IL-2-hypoxemia link) and targeted therapies (anti-TNF for vascular leakage). Future work should define hypoxia-specific cytokine networks, validate interventions in altitude cohorts, and integrate multi-omics to map immune-vascular crosstalk.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157005"},"PeriodicalIF":3.7,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cord blood chemokine levels as a predictor of oxidative stress and morbidities of prematurity","authors":"Gozdem Kayki ,&nbsp;Erdal Sag ,&nbsp;Ferid Aliyev ,&nbsp;Seza Ozen ,&nbsp;Sule Yigit","doi":"10.1016/j.cyto.2025.157006","DOIUrl":"10.1016/j.cyto.2025.157006","url":null,"abstract":"<div><h3>Objective</h3><div>Oxidative stress during the antenatal period causes an increase in the risk of morbidity and mortality in newborns. This study aimed to determine oxidative stress in the antenatal period, by measuring chemokine levels in cord blood and to show the correlation between chemokine levels and prematurity morbidities.</div></div><div><h3>Methods</h3><div>Neonates born at or below 32 weeks of gestation at Hacettepe University Ihsan Dogramaci Children's Hospital or Ordu University Training and Research Hospital were included in the study. Cord blood samples were collected and IL-8, IP-10, eotaxin, TARC, MCP-1, MIP-1α, MIG, ENA-78, MIP-3α, GROα, I-TAC, MIP-1β levels were measured. Morbidities of prematurity [respiratory distress syndrome (RDS), bronchopulmonary dysplasia (BPD), patent ductus arteriosus (PDA), intraventricular hemorrhage (IVH), periventricular leukomalacia (PVL), necrotizing enterocolitis (NEC) and retinopathy of prematurity (ROP)] and mortality/discharge status were collected via the hospital records. Diagnosis was noted and their relationship with cord blood chemokine levels was examined.</div></div><div><h3>Results</h3><div>Total 55 patients with a mean gestational age of 29 weeks (±3) and a mean birth weight of 1303 (±479) grams were included in the study. IL-8 levels were significantly higher in cord blood samples from babies diagnosed with RDS, BPD, PDA, IVH, PVL, ROP and mortality. Elevated MIP-3α levels were associated with BPD, PDA, NEC, IVH, ROP, and mortality, while increased MIP-1β levels were observed in cases of RDS, BPD, and ROP. When the participants were divided into three groups according to their gestational age, a statistically significant increase in IL-8 (<em>p</em> &lt; 0.001), MIP-3α (<em>p</em> = 0.004) and MIP-1β (<em>p</em> = 0.016) levels was found as gestational age decreased.</div></div><div><h3>Conclusion</h3><div>Cord blood chemokine levels including IL-8, MIP-3α, and MIP-1β may serve as potential biomarkers for identifying infants at risk of prematurity-related morbidities. However, since these levels also vary with gestational age, their interpretation requires caution. Larger studies are needed to validate these findings.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157006"},"PeriodicalIF":3.7,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms of IFNL3 are associated with risk of immune checkpoint inhibitor-related pneumonitis in lung cancer patients","authors":"Shengzu Peng ,&nbsp;Zhihui Duan ,&nbsp;Kai Zhang,&nbsp;Tao Lu,&nbsp;Guanghua Zheng","doi":"10.1016/j.cyto.2025.157002","DOIUrl":"10.1016/j.cyto.2025.157002","url":null,"abstract":"<div><h3>Background</h3><div>Checkpoint inhibitor-related pneumonitis (CIP) is the most common fatal adverse reaction among lung cancer patients received immune checkpoint inhibitors (ICIs) therapy. IFN-λ3 has anti-tumor and immunomodulatory effects in lung cancer; however, little is known about the <em>IFNL3</em> polymorphisms in CIP susceptible population.</div></div><div><h3>Methods</h3><div>Five candidate <em>IFNL3</em> polymorphisms were genotyped in 102 CIP and 626 non-CIP lung cancer patients, and the serum level of IFN-λ3 was detected by ELISA among the participants.</div></div><div><h3>Results</h3><div>The minor allele rs12979860-T, rs12980275-G and rs8099917-G were correlated with 2.524, 3.158 and 3.932-fold raised risk of CIP, respectively (<em>p</em> &lt; 0.001). Moreover, subgroup analysis according to tobacco use history showed that rs12980275 was correlated with CIP susceptibility in both subgroups, while rs12979860 and rs8099917 were associated with CIP in smokers (<em>p</em> &lt; 0.005). Additionally, the serum concentration of IFN-λ3 in CIP group was obviously less than that in non-CIP group (<em>p</em> &lt; 0.0001), and CIP patients with mutation genotypes of rs12979860, rs12980275 or rs8099917 had lower level of IFN-λ3 compared with those carriers with wild genotypes (<em>p</em> &lt; 0.01).</div></div><div><h3>Conclusion</h3><div><em>IFNL3</em> rs129798060-TT, rs12980275-GG and rs8099917-GG genotypes were associated with lower serum level of IFN-λ3 and CIP susceptibility in lung cancer patients.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157002"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of rotating night shift work on immune homeostasis among hospital employees during the COVID-19 pandemic","authors":"Sheng-Che Lin ,&nbsp;Chiou-Feng Lin ,&nbsp;Szu-Yuan Wu ,&nbsp;Chih-Chieh Yang ,&nbsp;Wan-Ming Chen ,&nbsp;Po-Chun Tseng ,&nbsp;Fu-Chia Shih ,&nbsp;Josephine Diony Nanda ,&nbsp;Chia-Ling Chen","doi":"10.1016/j.cyto.2025.157003","DOIUrl":"10.1016/j.cyto.2025.157003","url":null,"abstract":"<div><h3>Background</h3><div>Night shifts may disrupt circadian rhythms and immunity, potentially leading to health issues such as increased susceptibility to infectious diseases and impaired vaccine responses. This concern is particularly relevant for hospital employees during the COVID-19 pandemic. Our study aims to investigate the immune homeostasis of hospital workers engaged in rotating night shifts during the pandemic.</div></div><div><h3>Methods</h3><div>Healthy hospital employees aged 18–60 were enrolled and divided into two groups: the day shift (DS) group (<em>n</em> = 19) and the rotating night shift (RNS) group (<em>n</em> = 40). Blood samples were collected for analysis, including complete blood count, biochemistry, cytokines, anti-SARS-CoV-2 S1 antibody (anti-S1 IgG) titers, and immune cell subsets.</div></div><div><h3>Results</h3><div>The RNS group exhibited skewed Th1 immunity, characterized by significant elevations in IL-6 and IL-22, increasing trends in TNF-α and the IFN-γ/IL-5 ratio, along with a significant reduction in IL-5. Both groups demonstrated comparable rates of COVID-19 infection, and all participants showed effectively boosted anti-S1 IgG titers following antigen exposure (natural infection or vaccination). However, the RNS had lower anti-S1 IgG titers 3 to 6 months after antigen exposure. Additionally, decreased proportions of naïve B, and NKT cells along with an increase in T cell subsets, were detected in the RNS group. A significant positive correlation was also found between anti-S1 IgG titers and the proportion of NKT cells.</div></div><div><h3>Conclusion</h3><div>These findings suggest that RNS work induces low-grade inflammation and disrupts immune homeostasis, possibly lowering long-term anti-S1 IgG level after COVID-19 antigen exposure.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157003"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the circulating levels of IL-33 in patients with osteoarthritis: role of rs1929992 variants","authors":"Nahid Alimoradi ,&nbsp;Fatemeh Jahankhah ,&nbsp;Habibollah Jokardarzi ,&nbsp;Mohammad Tahami ,&nbsp;Negar Firouzabadi","doi":"10.1016/j.cyto.2025.156996","DOIUrl":"10.1016/j.cyto.2025.156996","url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) is among the common chronic health conditions which impacts many aspects of an individual’s life from physical function to mental health. Inflammation, in the joint as well as system inflammation is among the many mechanisms hypothesized to be involved in OA. IL-33 which belongs to the IL-1 family, is considered as an alarmin in OA. IL-33 binds to a group of receptors to stimulate signaling among which is the ST2L receptor that induced the activation of NFƙb, thus, working as a booster of the inflammatory cascade. Many polymorphisms have been identified on the IL-33 gene which are assumed to be associated with inflammatory diseases.</div></div><div><h3>Methods</h3><div>In the current double-blind placebo-controlled clinical trial study, patients diagnosed with knee OA were randomly divided into two groups: one group received metformin and the other received a placebo for 16 consecutive weeks (starting at 0.5 g/day, increasing to 1 g/day at week two, and increasing to 1.5 g/day for the remaining 14 weeks). Besides the evaluation of the clinical response to metformin using the Knee Injury and OA Outcome Score (KOOS) questionnaire, the serum levels of IL-33 was measured using enzyme-linked immunosorbent assay (ELISA) kits before (time 0) and after treatment (month 4). Genetic polymorphism of rs1929992 was assessed using in extracted DNAs using PCR-RFLP method.</div></div><div><h3>Results</h3><div>Serum levels of IL-33 were significantly higher in OA patients compered to healthy individuals (P&lt;0.001). Mutant allele (G allele) of the rs1929992 was significantly associated with OA (P=0.028; OR=1.9; 95%CI: 1.02-3.6). AG+GG genotypes were also associated with OA (P=0.004; OR=3.8, 95%CI: 1.5-9.5). Metformin did not affect IL-33 levels (P&gt;0.05). Variants of rs1929992 were not associated with response to metformin (P&gt;0.05).</div></div><div><h3>Conclusion</h3><div>Our findings support the role of polymorphisms of IL-33 gene and circulating levels of IL-33 in OA. Drugs targeting IL-33 signaling pathway may propose beneficial effects in OA.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156996"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum cytokine pattern for the early identification of hemophagocytic lymphohistiocytosis in diffuse large B-cell lymphoma","authors":"Jing Ni,&nbsp;Dongmei Zou,&nbsp;Yaofang Cao,&nbsp;Yan Liu,&nbsp;Fang Fang,&nbsp;Xiaoli Chang,&nbsp;Wuhan Hui,&nbsp;Yixian Guo,&nbsp;Ronghua Hu,&nbsp;Hong Zhao,&nbsp;Li Su,&nbsp;Wanling Sun","doi":"10.1016/j.cyto.2025.157000","DOIUrl":"10.1016/j.cyto.2025.157000","url":null,"abstract":"<div><div>Hemophagocytic lymphohistiocytosis (HLH) is generally recognized as a rapidly progressive syndrome of excessive immune activation and a cytokine storm. Diffuse large B-cell lymphoma (DLBCL), a prevalent subgroup of non-Hodgkin lymphoma, represents a common trigger of HLH. Due to the overlap in clinical manifestations between HLH and the underlying lymphoma, it is challenging to identify secondary HLH (sHLH) in DLBCL patients early. To elucidate the differences between DLBCL with HLH (DLBCL-HLH) and DLBCL without HLH (DLBCL-non-HLH), we comparatively analyzed the clinical parameters and serum cytokines in patients with DLBCL-HLH or DLBCL-non-HLH. Serum levels of interleukin (IL)-2, IL-4, IL-6, IL-8, IL-1β, IL-17 A, IL-10, IL-12P70, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and IFN-α in 8 patients with DLBCL-HLH and 51 patients with DLBCL-non-HLH were measured by cytometric bead array. Compared to patients with DLBCL-non-HLH, those with DLBCL-HLH had significantly elevated lactate dehydrogenase (LDH) levels, increased serum ferritin levels, raised liver transaminases, a higher proportion of lymphoma bone marrow involvement, significantly decreased albumin levels, and reduced peripheral blood cell counts. Both in patients with DLBCL-HLH and those with DLBCL-non-HLH, serum levels of IL-6 and IL-10 were elevated. However, these cytokine levels were significantly higher among patients with DLBCL-HLH. In particular, when the level of IL-10 &gt; 180.70 pg/ml, the sensitivity and specificity of the diagnosis of DLBCL-HLH were 87.50 % and 96.08 %, respectively. Interestingly, in patients with DLBCL-non-HLH, we observed that IL-10 positively correlated with serum ferritin levels (<em>r</em> = 0.768, <em>P</em> &lt; 0.001), and negatively correlated with hemoglobin concentrations and platelet counts (<em>r</em> = −0.388, <em>P</em> = 0.005; <em>r</em> = −0.412, <em>P</em> = 0.003). Taken together, our results revealed that elevated serum IL-10 levels are significantly associated with sHLH in DLBCL patients, suggesting its potential as a diagnostic biomarker. High serum IL-10 levels, combined with bone marrow involvement by lymphoma, high lactate dehydrogenase, decreased albumin levels, and HLH diagnostic criteria such as increased ferritin levels and cytopenia, might boost the early identification of DLBCL-HLH.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157000"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of home-based multi-task exercise training on executive function and TNF/IL-10 ratio in postmenopausal women with diabetes","authors":"A. Jamali ,&nbsp;M. Molanouri Shamsi ,&nbsp;M. Behmanesh ,&nbsp;A. Kouhkan ,&nbsp;P. Hassani-Abharian ,&nbsp;M. Pourmohammad ,&nbsp;R. Negaresh ,&nbsp;H. Adibi ,&nbsp;S. Soudi","doi":"10.1016/j.cyto.2025.157001","DOIUrl":"10.1016/j.cyto.2025.157001","url":null,"abstract":"<div><div>Diabetes, via chronic metabolic and inflammatory dysregulation, may impair immune function and contribute to cognitive decline, particularly in executive functions. In this study, we examined changes in the cytokines IL-10 and TNF, as well as their balance, alongside executive function, physical performance, and diabetes-related indices following a combined, dual-task, home-based exercise training program. A total of 85 inactive women aged 50–75 years with type 2 diabetes were randomly assigned to either an exercise training group or a control group. During the pre- and post-test phases, assessments were conducted in both groups to measure executive function, physical performance, levels of IL-10 and TNF, their ratio, and diabetes-related indices. The dual-tasked home-based exercise program improved physical performance and body composition (<em>P</em> &lt; 0.05). Executive function significantly improved in the exercise group (Effect size (ES) = 0.44, P &lt; 0.05), accompanied by reductions in IL-10 (ES = 1.11, P &lt; 0.05) and TNF levels (ES = 0.76, P &lt; 0.05). However, no significant changes were observed in the balance of these two cytokines or diabetes-related indices (<em>P</em> &gt; 0.05), except for HDL levels (ES = 0.87, P &lt; 0.05). Based on the results of the present study, engaging in multi-task home-based exercise training can enhance executive function and reduce inflammatory cytokines in women with type 2 diabetes. However, this training did not significantly affect the balance between IL-10 and TNF, which may be due to the moderate intensity of the exercises. Further long-term studies that take into account additional factors—such as nutrition, medication use, and cognitive status—are warranted to gain a clearer understanding of the cytokine-modulating effects of dual-task exercise training in individuals with type 2 diabetes.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157001"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Cancer biomarkers and biochemical parameters across breast Cancer stages: Correlation with age and comorbidities","authors":"Omeed Darweesh ,&nbsp;Mohammed K.J. Alnori","doi":"10.1016/j.cyto.2025.156997","DOIUrl":"10.1016/j.cyto.2025.156997","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Breast cancer is a public health concern that threatens women globally. The study aimed to examine the levels of Interleukin-10 (IL-10), carcinoembryonic antigen (CEA) and cancer antigen 15–3 (CA 15–3), along with various biochemical parameters (ferritin, hemoglobin (Hb), glutathione (GSH), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and aspartate aminotransferase (GOT) at different stages of breast cancer patients. Secondly, to determine the effects of patients' ages and comorbidity on the levels of IL-10 and CEA.</div></div><div><h3>Methods</h3><div>This study was conducted at the Kirkuk Oncology and Hematology Centre in Iraq, from May to November 2024. The study population consisted of two groups: 284 women, diagnosed with various stages of breast cancer (T1N0M0, T2N1M0, and T3N2M1) and 60 healthy women served as the control group. One-way ANOVA was used to determine statistically significant differences between control and various cancer stages, and the Pearson Correlation Coefficient to assess the correlation between different variables.</div></div><div><h3>Results</h3><div>Our data indicated that IL-10 and CEA levels were destructively upregulated in BC patients; specifically, IL-10 and CEA were raised four-fold and seven-fold, respectively, compared to the control group (IL-10: 30.1 ± 2.1 vs. 7.3 ± 1.1 (pg/ml), respectively), (CEA: 6.3 ± 1.8 vs. 0.8 ± 0.1 (ng/ml) respectively). Furthermore, BC patients demonstrated high levels of IL-10 and CEA, irrespective of the cancer stages, patients' ages, and the presence of comorbidity. The levels of CA 15–3 and other biochemical parameters (ferritin, Hb, GSH, LDH, ALP, GGT, GOT) were raised significantly at all cancer stages compared to the control group (<em>p</em> &lt; 0.05), the values were within normal ranges. However, Hb was significantly reduced at all cancer stages compared to the control group (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>We concluded that breast cancer in women is associated with a destructive upregulation of IL-10 and CEA, irrespective of the disease stage, patients' ages, and the existence of comorbidity.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156997"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}