Cytokine最新文献

{"title":"Genetic polymorphisms of IFNL3 are associated with risk of immune checkpoint inhibitor-related pneumonitis in lung cancer patients","authors":"Shengzu Peng ,&nbsp;Zhihui Duan ,&nbsp;Kai Zhang,&nbsp;Tao Lu,&nbsp;Guanghua Zheng","doi":"10.1016/j.cyto.2025.157002","DOIUrl":"10.1016/j.cyto.2025.157002","url":null,"abstract":"<div><h3>Background</h3><div>Checkpoint inhibitor-related pneumonitis (CIP) is the most common fatal adverse reaction among lung cancer patients received immune checkpoint inhibitors (ICIs) therapy. IFN-λ3 has anti-tumor and immunomodulatory effects in lung cancer; however, little is known about the <em>IFNL3</em> polymorphisms in CIP susceptible population.</div></div><div><h3>Methods</h3><div>Five candidate <em>IFNL3</em> polymorphisms were genotyped in 102 CIP and 626 non-CIP lung cancer patients, and the serum level of IFN-λ3 was detected by ELISA among the participants.</div></div><div><h3>Results</h3><div>The minor allele rs12979860-T, rs12980275-G and rs8099917-G were correlated with 2.524, 3.158 and 3.932-fold raised risk of CIP, respectively (<em>p</em> &lt; 0.001). Moreover, subgroup analysis according to tobacco use history showed that rs12980275 was correlated with CIP susceptibility in both subgroups, while rs12979860 and rs8099917 were associated with CIP in smokers (<em>p</em> &lt; 0.005). Additionally, the serum concentration of IFN-λ3 in CIP group was obviously less than that in non-CIP group (<em>p</em> &lt; 0.0001), and CIP patients with mutation genotypes of rs12979860, rs12980275 or rs8099917 had lower level of IFN-λ3 compared with those carriers with wild genotypes (<em>p</em> &lt; 0.01).</div></div><div><h3>Conclusion</h3><div><em>IFNL3</em> rs129798060-TT, rs12980275-GG and rs8099917-GG genotypes were associated with lower serum level of IFN-λ3 and CIP susceptibility in lung cancer patients.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157002"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of rotating night shift work on immune homeostasis among hospital employees during the COVID-19 pandemic","authors":"Sheng-Che Lin ,&nbsp;Chiou-Feng Lin ,&nbsp;Szu-Yuan Wu ,&nbsp;Chih-Chieh Yang ,&nbsp;Wan-Ming Chen ,&nbsp;Po-Chun Tseng ,&nbsp;Fu-Chia Shih ,&nbsp;Josephine Diony Nanda ,&nbsp;Chia-Ling Chen","doi":"10.1016/j.cyto.2025.157003","DOIUrl":"10.1016/j.cyto.2025.157003","url":null,"abstract":"<div><h3>Background</h3><div>Night shifts may disrupt circadian rhythms and immunity, potentially leading to health issues such as increased susceptibility to infectious diseases and impaired vaccine responses. This concern is particularly relevant for hospital employees during the COVID-19 pandemic. Our study aims to investigate the immune homeostasis of hospital workers engaged in rotating night shifts during the pandemic.</div></div><div><h3>Methods</h3><div>Healthy hospital employees aged 18–60 were enrolled and divided into two groups: the day shift (DS) group (<em>n</em> = 19) and the rotating night shift (RNS) group (<em>n</em> = 40). Blood samples were collected for analysis, including complete blood count, biochemistry, cytokines, anti-SARS-CoV-2 S1 antibody (anti-S1 IgG) titers, and immune cell subsets.</div></div><div><h3>Results</h3><div>The RNS group exhibited skewed Th1 immunity, characterized by significant elevations in IL-6 and IL-22, increasing trends in TNF-α and the IFN-γ/IL-5 ratio, along with a significant reduction in IL-5. Both groups demonstrated comparable rates of COVID-19 infection, and all participants showed effectively boosted anti-S1 IgG titers following antigen exposure (natural infection or vaccination). However, the RNS had lower anti-S1 IgG titers 3 to 6 months after antigen exposure. Additionally, decreased proportions of naïve B, and NKT cells along with an increase in T cell subsets, were detected in the RNS group. A significant positive correlation was also found between anti-S1 IgG titers and the proportion of NKT cells.</div></div><div><h3>Conclusion</h3><div>These findings suggest that RNS work induces low-grade inflammation and disrupts immune homeostasis, possibly lowering long-term anti-S1 IgG level after COVID-19 antigen exposure.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157003"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the circulating levels of IL-33 in patients with osteoarthritis: role of rs1929992 variants","authors":"Nahid Alimoradi ,&nbsp;Fatemeh Jahankhah ,&nbsp;Habibollah Jokardarzi ,&nbsp;Mohammad Tahami ,&nbsp;Negar Firouzabadi","doi":"10.1016/j.cyto.2025.156996","DOIUrl":"10.1016/j.cyto.2025.156996","url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) is among the common chronic health conditions which impacts many aspects of an individual’s life from physical function to mental health. Inflammation, in the joint as well as system inflammation is among the many mechanisms hypothesized to be involved in OA. IL-33 which belongs to the IL-1 family, is considered as an alarmin in OA. IL-33 binds to a group of receptors to stimulate signaling among which is the ST2L receptor that induced the activation of NFƙb, thus, working as a booster of the inflammatory cascade. Many polymorphisms have been identified on the IL-33 gene which are assumed to be associated with inflammatory diseases.</div></div><div><h3>Methods</h3><div>In the current double-blind placebo-controlled clinical trial study, patients diagnosed with knee OA were randomly divided into two groups: one group received metformin and the other received a placebo for 16 consecutive weeks (starting at 0.5 g/day, increasing to 1 g/day at week two, and increasing to 1.5 g/day for the remaining 14 weeks). Besides the evaluation of the clinical response to metformin using the Knee Injury and OA Outcome Score (KOOS) questionnaire, the serum levels of IL-33 was measured using enzyme-linked immunosorbent assay (ELISA) kits before (time 0) and after treatment (month 4). Genetic polymorphism of rs1929992 was assessed using in extracted DNAs using PCR-RFLP method.</div></div><div><h3>Results</h3><div>Serum levels of IL-33 were significantly higher in OA patients compered to healthy individuals (P&lt;0.001). Mutant allele (G allele) of the rs1929992 was significantly associated with OA (P=0.028; OR=1.9; 95%CI: 1.02-3.6). AG+GG genotypes were also associated with OA (P=0.004; OR=3.8, 95%CI: 1.5-9.5). Metformin did not affect IL-33 levels (P&gt;0.05). Variants of rs1929992 were not associated with response to metformin (P&gt;0.05).</div></div><div><h3>Conclusion</h3><div>Our findings support the role of polymorphisms of IL-33 gene and circulating levels of IL-33 in OA. Drugs targeting IL-33 signaling pathway may propose beneficial effects in OA.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156996"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum cytokine pattern for the early identification of hemophagocytic lymphohistiocytosis in diffuse large B-cell lymphoma","authors":"Jing Ni,&nbsp;Dongmei Zou,&nbsp;Yaofang Cao,&nbsp;Yan Liu,&nbsp;Fang Fang,&nbsp;Xiaoli Chang,&nbsp;Wuhan Hui,&nbsp;Yixian Guo,&nbsp;Ronghua Hu,&nbsp;Hong Zhao,&nbsp;Li Su,&nbsp;Wanling Sun","doi":"10.1016/j.cyto.2025.157000","DOIUrl":"10.1016/j.cyto.2025.157000","url":null,"abstract":"<div><div>Hemophagocytic lymphohistiocytosis (HLH) is generally recognized as a rapidly progressive syndrome of excessive immune activation and a cytokine storm. Diffuse large B-cell lymphoma (DLBCL), a prevalent subgroup of non-Hodgkin lymphoma, represents a common trigger of HLH. Due to the overlap in clinical manifestations between HLH and the underlying lymphoma, it is challenging to identify secondary HLH (sHLH) in DLBCL patients early. To elucidate the differences between DLBCL with HLH (DLBCL-HLH) and DLBCL without HLH (DLBCL-non-HLH), we comparatively analyzed the clinical parameters and serum cytokines in patients with DLBCL-HLH or DLBCL-non-HLH. Serum levels of interleukin (IL)-2, IL-4, IL-6, IL-8, IL-1β, IL-17 A, IL-10, IL-12P70, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and IFN-α in 8 patients with DLBCL-HLH and 51 patients with DLBCL-non-HLH were measured by cytometric bead array. Compared to patients with DLBCL-non-HLH, those with DLBCL-HLH had significantly elevated lactate dehydrogenase (LDH) levels, increased serum ferritin levels, raised liver transaminases, a higher proportion of lymphoma bone marrow involvement, significantly decreased albumin levels, and reduced peripheral blood cell counts. Both in patients with DLBCL-HLH and those with DLBCL-non-HLH, serum levels of IL-6 and IL-10 were elevated. However, these cytokine levels were significantly higher among patients with DLBCL-HLH. In particular, when the level of IL-10 &gt; 180.70 pg/ml, the sensitivity and specificity of the diagnosis of DLBCL-HLH were 87.50 % and 96.08 %, respectively. Interestingly, in patients with DLBCL-non-HLH, we observed that IL-10 positively correlated with serum ferritin levels (<em>r</em> = 0.768, <em>P</em> &lt; 0.001), and negatively correlated with hemoglobin concentrations and platelet counts (<em>r</em> = −0.388, <em>P</em> = 0.005; <em>r</em> = −0.412, <em>P</em> = 0.003). Taken together, our results revealed that elevated serum IL-10 levels are significantly associated with sHLH in DLBCL patients, suggesting its potential as a diagnostic biomarker. High serum IL-10 levels, combined with bone marrow involvement by lymphoma, high lactate dehydrogenase, decreased albumin levels, and HLH diagnostic criteria such as increased ferritin levels and cytopenia, might boost the early identification of DLBCL-HLH.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157000"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of home-based multi-task exercise training on executive function and TNF/IL-10 ratio in postmenopausal women with diabetes","authors":"A. Jamali ,&nbsp;M. Molanouri Shamsi ,&nbsp;M. Behmanesh ,&nbsp;A. Kouhkan ,&nbsp;P. Hassani-Abharian ,&nbsp;M. Pourmohammad ,&nbsp;R. Negaresh ,&nbsp;H. Adibi ,&nbsp;S. Soudi","doi":"10.1016/j.cyto.2025.157001","DOIUrl":"10.1016/j.cyto.2025.157001","url":null,"abstract":"<div><div>Diabetes, via chronic metabolic and inflammatory dysregulation, may impair immune function and contribute to cognitive decline, particularly in executive functions. In this study, we examined changes in the cytokines IL-10 and TNF, as well as their balance, alongside executive function, physical performance, and diabetes-related indices following a combined, dual-task, home-based exercise training program. A total of 85 inactive women aged 50–75 years with type 2 diabetes were randomly assigned to either an exercise training group or a control group. During the pre- and post-test phases, assessments were conducted in both groups to measure executive function, physical performance, levels of IL-10 and TNF, their ratio, and diabetes-related indices. The dual-tasked home-based exercise program improved physical performance and body composition (<em>P</em> &lt; 0.05). Executive function significantly improved in the exercise group (Effect size (ES) = 0.44, P &lt; 0.05), accompanied by reductions in IL-10 (ES = 1.11, P &lt; 0.05) and TNF levels (ES = 0.76, P &lt; 0.05). However, no significant changes were observed in the balance of these two cytokines or diabetes-related indices (<em>P</em> &gt; 0.05), except for HDL levels (ES = 0.87, P &lt; 0.05). Based on the results of the present study, engaging in multi-task home-based exercise training can enhance executive function and reduce inflammatory cytokines in women with type 2 diabetes. However, this training did not significantly affect the balance between IL-10 and TNF, which may be due to the moderate intensity of the exercises. Further long-term studies that take into account additional factors—such as nutrition, medication use, and cognitive status—are warranted to gain a clearer understanding of the cytokine-modulating effects of dual-task exercise training in individuals with type 2 diabetes.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 157001"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Cancer biomarkers and biochemical parameters across breast Cancer stages: Correlation with age and comorbidities","authors":"Omeed Darweesh ,&nbsp;Mohammed K.J. Alnori","doi":"10.1016/j.cyto.2025.156997","DOIUrl":"10.1016/j.cyto.2025.156997","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Breast cancer is a public health concern that threatens women globally. The study aimed to examine the levels of Interleukin-10 (IL-10), carcinoembryonic antigen (CEA) and cancer antigen 15–3 (CA 15–3), along with various biochemical parameters (ferritin, hemoglobin (Hb), glutathione (GSH), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and aspartate aminotransferase (GOT) at different stages of breast cancer patients. Secondly, to determine the effects of patients' ages and comorbidity on the levels of IL-10 and CEA.</div></div><div><h3>Methods</h3><div>This study was conducted at the Kirkuk Oncology and Hematology Centre in Iraq, from May to November 2024. The study population consisted of two groups: 284 women, diagnosed with various stages of breast cancer (T1N0M0, T2N1M0, and T3N2M1) and 60 healthy women served as the control group. One-way ANOVA was used to determine statistically significant differences between control and various cancer stages, and the Pearson Correlation Coefficient to assess the correlation between different variables.</div></div><div><h3>Results</h3><div>Our data indicated that IL-10 and CEA levels were destructively upregulated in BC patients; specifically, IL-10 and CEA were raised four-fold and seven-fold, respectively, compared to the control group (IL-10: 30.1 ± 2.1 vs. 7.3 ± 1.1 (pg/ml), respectively), (CEA: 6.3 ± 1.8 vs. 0.8 ± 0.1 (ng/ml) respectively). Furthermore, BC patients demonstrated high levels of IL-10 and CEA, irrespective of the cancer stages, patients' ages, and the presence of comorbidity. The levels of CA 15–3 and other biochemical parameters (ferritin, Hb, GSH, LDH, ALP, GGT, GOT) were raised significantly at all cancer stages compared to the control group (<em>p</em> &lt; 0.05), the values were within normal ranges. However, Hb was significantly reduced at all cancer stages compared to the control group (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>We concluded that breast cancer in women is associated with a destructive upregulation of IL-10 and CEA, irrespective of the disease stage, patients' ages, and the existence of comorbidity.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156997"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appetite adipokines and insulin resistance shape the link between sleep quality and cardiac structure in youths with cardiometabolic risk: the BCAMS study","authors":"Mengyu He ,&nbsp;Ling Zhong ,&nbsp;Lanwen Han ,&nbsp;Xinghao Yi ,&nbsp;Ming Li ,&nbsp;Shan Gao","doi":"10.1016/j.cyto.2025.156998","DOIUrl":"10.1016/j.cyto.2025.156998","url":null,"abstract":"<div><h3>Background</h3><div>Poor sleep quality (PSQ) is associated with cardiovascular disease, but the key intermediate variables underlying this relationship in youths remain unclear. We aimed to explore the relationship between PSQ and adverse cardiac structure in Chinese youths with elevated cardiometabolic risk, focusing on the roles of appetite adipokines and insulin resistance.</div></div><div><h3>Methods</h3><div>We utilized cross-sectional data from the Beijing Children and Adolescents Metabolic Syndrome (BCAMS) Study Cohort (<em>n</em> = 559, mean age = 20.2 years). Participants underwent echocardiographic assessments, a sleep quality questionnaire, an oral glucose tolerance test, and plasma levels of insulin and five appetite adipokines.</div></div><div><h3>Results</h3><div>Elevated PSQ scores were associated with adverse left ventricular mass index (LVMI) (0.64 g/m^2.7 per unit increase in PSQ score), altered appetite adipokines, including 8.3 % higher leptin, 1.9 % higher retinol-binding protein 4 (RBP4), and 3.2 % lower high-molecular-weight adiponectin (HMW-adiponectin), as well as impaired insulin sensitivity, reflected by 6.6 % higher fasting insulin levels, 6.7 % higher homeostasis model assessment of insulin resistance (HOMA-IR), and 4.9 % lower insulin sensitivity index (Matsuda Index) (ISI_Matsuda), per point increase in PSQ score (all <em>p</em> &lt; 0.05). In mediation analyses, leptin significantly mediated 28.5 % of the PSQ–LVMI association (<em>p</em> &lt; 0.001), and insulin-related indices explained 13.6 %–23.6 % (<em>p</em> &lt; 0.05); HMW-adiponectin showed a marginal mediating effect (8.1 %, <em>p</em> = 0.077). Furthermore, interaction analysis revealed that insulin resistance amplified the adverse impact of PSQ on LVMI (<em>p</em>_interaction = 0.04 for HOMA-IR; 0.029 for ISI_Matsuda), with high PSQ-related elevations in LVMI more pronounced among youths with higher insulin resistance.</div></div><div><h3>Conclusions</h3><div>PSQ is associated with early adverse cardiac remodeling in youths with cardiometabolic risk, potentially driven by elevated leptin and insulin resistance. Targeting sleep quality, adipokine signaling, and insulin sensitivity, may offer synergistic strategies for early cardiovascular prevention in at-risk youths.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156998"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative mRNA expression signature of PBMC-derived NLRP3 inflammasomes in progression of type 2 diabetic kidney disease","authors":"Jayashree Kuppuswami ,&nbsp;Gandhipuram Periyasamy Senthilkumar ,&nbsp;Sreejith Parameswaran ,&nbsp;Mehalingam Vadivelan","doi":"10.1016/j.cyto.2025.156999","DOIUrl":"10.1016/j.cyto.2025.156999","url":null,"abstract":"<div><h3>Background</h3><div>NLRP3 inflammasome triggered by chronic hyperglycemia in resident pro-inflammatory leucocytes of the diabetic kidney is predominant in perpetuating chronic inflammation and renal fibrosis. This study focuses on NLRP3 expressed in circulatory immune cells, to elucidate the systemic level significance of NLRP3 inflammasome in contributing to diabetic kidney disease (DKD) progression. We therefore, aim to investigate the mRNA expression signature of genes encoding the NLRP3 inflammasome complex in circulatory immune cells and its association with severity of DKD in type 2 diabetes mellitus (T2DM) patients.</div></div><div><h3>Materials &amp; methods</h3><div>PBMCs were isolated from the blood samples of 160 T2DM patients presenting with varying grades of DKD severity. RNA extracted from the PBMCs was used for relative quantification of our target genes using SYBRGreen real-time RT-qPCR. Association of DKD progression with NLRP3 component genes' mRNA expression was analyzed statistically.</div></div><div><h3>Results</h3><div>Expression of <em>NLRP3, CASP1</em>, and <em>IL1β</em> mRNAs were significantly elevated in DKD; higher <em>HSP72</em> mRNA expression accompanied normoalbuminuric T2DM. PBMC-derived <em>NLRP3</em> mRNA levels correlated positively with circulatory monocyte population. Plasma NLRP3 protein and NLRP3 mRNA expressed in PBMCs were associated inversely with decreasing eGFR, independent of diabetes duration, age and sex. Contrastingly, <em>HSP72</em> mRNA levels declined with decline in eGFR. No association between NLRP3 complex genes and <em>HSP72</em> mRNA expression in PBMCs was observed, although <em>HSP72</em> exhibited inverse characteristics to that of <em>NLRP3</em> complex in the study.</div></div><div><h3>Conclusion</h3><div>A dynamic interplay exists between DKD progression and PBMC-derived mRNA expression of NLRP3 inflammasome and <em>HSP72</em>. At the systemic level, while NLRP3 exasperates inflammation, the protective role of HSP72 is extinguished. Their expression patterns in circulatory immune cells are associated with eGFR decline, tying systemic inflammatory state to worsening kidney disease outcomes.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156999"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifaceted approach to lupus nephritis: Deciphering the interplay between adipokines, cytokines and complement proteins in disease pathogenesis","authors":"Durga Chougule ,&nbsp;Anjali Rajadhyaksha ,&nbsp;Tukaram Jamale ,&nbsp;Kalpana Mehta ,&nbsp;Amrutha Jose ,&nbsp;Swapnal Pawaskar ,&nbsp;Manisha Madkaikar ,&nbsp;Vandana Pradhan","doi":"10.1016/j.cyto.2025.156992","DOIUrl":"10.1016/j.cyto.2025.156992","url":null,"abstract":"<div><h3>Background</h3><div>The pro- and anti-inflammatory adipokines secreted by closely located visceral adipose tissue (VAT) have showed to modulate the inflammatory milieu around kidneys. In this study, we investigate the interplay between adipokines, cytokines and complement proteins in lupus nephritis patients.</div></div><div><h3>Materials and methods</h3><div>Treatment naïve renal biopsy proven (2003 ISN/RPS) LN patients (<em>n</em> = 72) and healthy volunteers (<em>n</em> = 65) were enrolled (January 2017–January 2021). Serum adipokines, cytokines, complement proteins, C- reactive protein, Antinuclear antibodies (ANA) profile, anti-dsDNA antibodies and anti-phospholipid antibody (APA) profile were estimated using immunoassay. Serum C3 and C4 levels were measured by nephelometer.</div></div><div><h3>Results</h3><div>Elevated adiponectin, adipsin, resistin, progranulin, galectin-3 and pentraxin-3 levels and reduced leptin levels were seen in LN patients when compared to healthy controls <strong>(<em>p</em></strong> <strong>&lt;</strong> <strong>0.05)</strong>. Serum IL-1β, INFγ, IL-6 and MCP-1 levels were significantly elevated whereas IL-4 levels were prominently reduced in LN patients when compared with healthy controls <strong>(<em>p</em></strong> <strong>&lt;</strong> <strong>0.05)</strong>. Serum C1q and CFP levels were significantly reduced in LN patients <strong>(<em>p</em></strong> <strong>&lt;</strong> <strong>0.05)</strong>. Elevated pentraxin-3 levels directly correlated with long disease duration and high SLEDAI score <strong>(<em>p</em></strong> <strong>&lt;</strong> <strong>0.05)</strong>. Declining renal function parameters strongly correlated with raised adiponectin, adipsin and resistin levels <strong>(<em>p</em></strong> <strong>&lt;</strong> <strong>0.05)</strong>. Adipsin levels positively correlated with complement components C3, C4 and CFB levels <strong>(p</strong> <strong>&lt;</strong> <strong>0.05)</strong>. The PCA of serum proteins in LN patients identified three distinct clusters comprising of 37 (Cluster1), 24 (Cluster2) and 1 (Cluster3) patients in each. Adiponectin, resistin and CRP levels were found to be elevated in patients belonging to Cluster1. On the contrary, MCP1 levels were raised in Cluster2 LN patients.</div></div><div><h3>Conclusion</h3><div>The association of raised resistin, adipsin, pentraxin-3 levels with deteriorating renal function is suggestive of their potential role in LN pathogenesis. The association between adipokines and complement components indicates the regulatory role of adipokines on complements and vice versa. The identification of patient clusters based on serum protein profile indicated involvement of unique biological profiles in diverse LN pathogenesis mechanism.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156992"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between aqueous humor cytokines and posterior capsular opacification in patients with high myopia","authors":"Lu Cheng ,&nbsp;Shiqi Xie ,&nbsp;Huiyi Jin ,&nbsp;Yanyun Lv ,&nbsp;Bijun Zhu ,&nbsp;Haidong Zou ,&nbsp;Baojiang Li ,&nbsp;Peiyao Jin","doi":"10.1016/j.cyto.2025.156995","DOIUrl":"10.1016/j.cyto.2025.156995","url":null,"abstract":"<div><h3>Background</h3><div>Posterior capsular opacification (PCO) remains a significant complication following cataract surgery, with an unclear pathogenesis in patients with high myopia. This study investigated the aqueous humor (AH) cytokine profile in patients with cataracts and high myopia and its relationship with the development of PCO.</div></div><div><h3>Methods</h3><div>AH samples were collected from 36 patients undergoing cataract surgery, including 18 with high myopia (axial length &gt; 26 mm) and 18 age- and sex-matched controls. The levels of 19 PCO-associated cytokines were measured using a multiplex immunoassay, and PCO severity was assessed at 6 months postoperatively.</div></div><div><h3>Results</h3><div>Compared to controls, patients with high myopia exhibited significantly elevated levels of vascular endothelial growth factor (VEGF)-C, hepatocyte growth factor (HGF), monocyte chemoattractant protein-1 (MCP-1), and osteopontin, alongside reduced VEGF-A levels (all <em>P</em> &lt; 0.01). Axial length correlated positively with VEGF-C, HGF, MCP-1, and osteopontin but negatively with VEGF-A. Increased PCO severity was associated with higher VEGF-C, HGF, MCP-1, and osteopontin levels, while VEGF-A levels were inversely related.</div></div><div><h3>Conclusion</h3><div>These findings suggest that high myopia is linked to a distinct pro-inflammatory and pro-fibrotic cytokine environment, potentially exacerbating PCO. Identifying these cytokines as potential biomarkers may aid in early risk stratification and inform therapeutic strategies to prevent PCO in individuals with high myopia.</div></div>","PeriodicalId":297,"journal":{"name":"Cytokine","volume":"194 ","pages":"Article 156995"},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}