ACS Nanoscience Au最新文献

{"title":"","authors":"Kristen M. Aviles,  and , Benjamin J. Lear*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsnanoscienceau.4c00076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144355181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why Nanoscience Needs Standardized ProtocolsAnd How to Get There.","authors":"Marek Grzelczak","doi":"10.1021/acsnanoscienceau.5c00028","DOIUrl":"10.1021/acsnanoscienceau.5c00028","url":null,"abstract":"<p><p>Nanoscience is a relatively young research field that has been built on the shoulders of consolidated areas ranging from solid-state physics to biology. Its interdisciplinary nature imposes the flow of heterogeneous data from various domains of predefined conventions that ultimately prevents workflow standardization, raising the possibility of further fragmentation and compromising the reproducibility. This is the time to establish good practices for experimental nanoscientists. This work proposes a set of simple rules that can facilitate data management and improve their reusability. Implementing the proposed protocol can have high initial cognitive costs but can also save energy and time in the long term. By adopting these practices, researchers can ensure the reusability of their data early in a project and accelerate the writing process.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"112-116"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why Nanoscience Needs Standardized Protocols─And How to Get There","authors":"Marek Grzelczak*,&nbsp;","doi":"10.1021/acsnanoscienceau.5c0002810.1021/acsnanoscienceau.5c00028","DOIUrl":"https://doi.org/10.1021/acsnanoscienceau.5c00028https://doi.org/10.1021/acsnanoscienceau.5c00028","url":null,"abstract":"<p >Nanoscience is a relatively young research field that has been built on the shoulders of consolidated areas ranging from solid-state physics to biology. Its interdisciplinary nature imposes the flow of heterogeneous data from various domains of predefined conventions that ultimately prevents workflow standardization, raising the possibility of further fragmentation and compromising the reproducibility. This is the time to establish good practices for experimental nanoscientists. This work proposes a set of simple rules that can facilitate data management and improve their reusability. Implementing the proposed protocol can have high initial cognitive costs but can also save energy and time in the long term. By adopting these practices, researchers can ensure the reusability of their data early in a project and accelerate the writing process.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"112–116 112–116"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.5c00028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Element-Selective Local Structures in Multielement Alloy Nanoparticles with EXAFS","authors":"Masashi Nakamura*,&nbsp;Dongshuang Wu*,&nbsp;Megumi Mukoyoshi*,&nbsp;Kohei Kusada,&nbsp;Hiroyuki Hayashi,&nbsp;Takaaki Toriyama,&nbsp;Tomokazu Yamamoto,&nbsp;Yasukazu Murakami,&nbsp;Hirotaka Ashitani,&nbsp;Shogo Kawaguchi,&nbsp;Toshiaki Ina,&nbsp;Osami Sakata,&nbsp;Yoshiki Kubota,&nbsp;Isao Tanaka and Hiroshi Kitagawa*,&nbsp;","doi":"10.1021/acsnanoscienceau.5c0001310.1021/acsnanoscienceau.5c00013","DOIUrl":"https://doi.org/10.1021/acsnanoscienceau.5c00013https://doi.org/10.1021/acsnanoscienceau.5c00013","url":null,"abstract":"<p >We demonstrate physically consistent and interpretable extended X-ray absorption fine structure (EXAFS) curve-fitting analyses for estimating element-selective local structures in multielement alloy nanoparticles (MEA NPs). The difficulty in analyzing multielement systems originates from the too large number of independent structural parameters to fit, far exceeding the information content of the typical experimental data. Herein, this challenge is overcome by simultaneously fitting multiple data at different absorption edges and temperatures while imposing constraints based on a physically reasonable model. Another advantage of our approach is interpretability; the individual contributions of the constituent elements to the static and dynamic structures are explicitly estimated as atomic radii and Einstein temperatures. This method is used to analyze MEA NPs composed of platinum-group metals and <i>p</i>-block metals, which have contrasting properties, including atomic radii, melting points, and electronegativities. The results indicate that the local structures reflect the intrinsic nature of the elements and are also influenced by the interactions among them. The local structures around the <i>p</i>-block metals in the MEA NPs are shown to be distinctively modulated compared with those in the corresponding monometals, which is attributed to the electronic interaction with the platinum-group metals based on <i>ab initio</i> calculations. Our method is expected to facilitate the experimental characterization of these structurally complicated nanomaterials, which have been analyzed relying on calculations, yielding more precise pictures of real systems for investigating structure–property relationships.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"196–207 196–207"},"PeriodicalIF":4.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.5c00013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Element-Selective Local Structures in Multielement Alloy Nanoparticles with EXAFS.","authors":"Masashi Nakamura, Dongshuang Wu, Megumi Mukoyoshi, Kohei Kusada, Hiroyuki Hayashi, Takaaki Toriyama, Tomokazu Yamamoto, Yasukazu Murakami, Hirotaka Ashitani, Shogo Kawaguchi, Toshiaki Ina, Osami Sakata, Yoshiki Kubota, Isao Tanaka, Hiroshi Kitagawa","doi":"10.1021/acsnanoscienceau.5c00013","DOIUrl":"10.1021/acsnanoscienceau.5c00013","url":null,"abstract":"<p><p>We demonstrate physically consistent and interpretable extended X-ray absorption fine structure (EXAFS) curve-fitting analyses for estimating element-selective local structures in multielement alloy nanoparticles (MEA NPs). The difficulty in analyzing multielement systems originates from the too large number of independent structural parameters to fit, far exceeding the information content of the typical experimental data. Herein, this challenge is overcome by simultaneously fitting multiple data at different absorption edges and temperatures while imposing constraints based on a physically reasonable model. Another advantage of our approach is interpretability; the individual contributions of the constituent elements to the static and dynamic structures are explicitly estimated as atomic radii and Einstein temperatures. This method is used to analyze MEA NPs composed of platinum-group metals and <i>p</i>-block metals, which have contrasting properties, including atomic radii, melting points, and electronegativities. The results indicate that the local structures reflect the intrinsic nature of the elements and are also influenced by the interactions among them. The local structures around the <i>p</i>-block metals in the MEA NPs are shown to be distinctively modulated compared with those in the corresponding monometals, which is attributed to the electronic interaction with the platinum-group metals based on <i>ab initio</i> calculations. Our method is expected to facilitate the experimental characterization of these structurally complicated nanomaterials, which have been analyzed relying on calculations, yielding more precise pictures of real systems for investigating structure-property relationships.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"196-207"},"PeriodicalIF":4.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular Uptake of Hybrid PLGA-Lipid Gadolinium Nanoparticles Functionalized for Magnetic Resonance Imaging of Pancreatic Adenocarcinoma Cells","authors":"Alessandro Amaolo,&nbsp;Hanieh Sadeghi,&nbsp;Carla Carrera,&nbsp;Sergio Padovan,&nbsp;Fabio Carniato,&nbsp;Enza Di Gregorio and Giuseppe Ferrauto*,&nbsp;","doi":"10.1021/acsnanoscienceau.5c0001010.1021/acsnanoscienceau.5c00010","DOIUrl":"https://doi.org/10.1021/acsnanoscienceau.5c00010https://doi.org/10.1021/acsnanoscienceau.5c00010","url":null,"abstract":"<p >Pancreatic adenocarcinoma (PDAC) presents significant diagnostic challenges, necessitating improved imaging techniques. Here, we develop hybrid poly(lactic-<i>co</i>-glycolic acid) (PLGA)-phospholipid nanoparticles (NPs) loaded with gadolinium (Gd) chelates and functionalized with albumin, adenosine, or glutamine to boost their internalization in PDAC cells and increase the detectability by magnetic resonance imaging (MRI). Gd-PLGA NPs were synthesized using an <i>oil-in-water</i> emulsion solvent extraction method and incorporating DSPE-PEG(2000)methoxy and DPPE-PEG(2000) <i>N</i>-Hydroxysuccinimide (NHS) for surface functionalization with albumin, adenosine, or glutamine. NPs were characterized by dynamic light scattering for particle size and ζ potential measurements, in addition to ¹H NMR and proton nuclear magnetic relaxation dispersion to assess relaxivity and contrastographic properties, and stability studies were conducted in both HEPES-buffered saline and human serum. Reported studies demonstrated that all the preparations display a good stability, a hydrodynamic diameter lower than 200 nm, and a slight negative surface charge, with good potential for applications in cells and in vivo. In vitro studies on MiaPaca2 and Panc1 cell lines confirmed that functionalized NPs display higher cellular uptake and stronger MRI signal enhancement than unconjugated controls, with albumin-PLGA-lipid NPs leading to the greatest uptake. Our findings highlight a promising route toward a more sensitive, targeted MRI of PDAC, calling for in vivo studies to assess diagnostic potential and therapeutic applications.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"184–195 184–195"},"PeriodicalIF":4.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.5c00010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular Uptake of Hybrid PLGA-Lipid Gadolinium Nanoparticles Functionalized for Magnetic Resonance Imaging of Pancreatic Adenocarcinoma Cells.","authors":"Alessandro Amaolo, Hanieh Sadeghi, Carla Carrera, Sergio Padovan, Fabio Carniato, Enza Di Gregorio, Giuseppe Ferrauto","doi":"10.1021/acsnanoscienceau.5c00010","DOIUrl":"10.1021/acsnanoscienceau.5c00010","url":null,"abstract":"<p><p>Pancreatic adenocarcinoma (PDAC) presents significant diagnostic challenges, necessitating improved imaging techniques. Here, we develop hybrid poly-(lactic-<i>co</i>-glycolic acid) (PLGA)-phospholipid nanoparticles (NPs) loaded with gadolinium (Gd) chelates and functionalized with albumin, adenosine, or glutamine to boost their internalization in PDAC cells and increase the detectability by magnetic resonance imaging (MRI). Gd-PLGA NPs were synthesized using an <i>oil-in-water</i> emulsion solvent extraction method and incorporating DSPE-PEG(2000)-methoxy and DPPE-PEG(2000) <i>N</i>-Hydroxysuccinimide (NHS) for surface functionalization with albumin, adenosine, or glutamine. NPs were characterized by dynamic light scattering for particle size and ζ potential measurements, in addition to ¹H NMR and proton nuclear magnetic relaxation dispersion to assess relaxivity and contrastographic properties, and stability studies were conducted in both HEPES-buffered saline and human serum. Reported studies demonstrated that all the preparations display a good stability, a hydrodynamic diameter lower than 200 nm, and a slight negative surface charge, with good potential for applications in cells and in vivo. In vitro studies on MiaPaca2 and Panc1 cell lines confirmed that functionalized NPs display higher cellular uptake and stronger MRI signal enhancement than unconjugated controls, with albumin-PLGA-lipid NPs leading to the greatest uptake. Our findings highlight a promising route toward a more sensitive, targeted MRI of PDAC, calling for in vivo studies to assess diagnostic potential and therapeutic applications.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"184-195"},"PeriodicalIF":4.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine's Impact on Apoptosis, Proliferation, Uptake Efficiency, and Nanoparticle-Based Therapy in DBTRG Cells.","authors":"Chiung-Chyi Shen, Meng-Yin Yang, Wan-Yu Hsieh, Gregory J Tsay, Yi-Chin Yang, Yu-Fen Huang, Szu-Yuan Liu, Chih-Ming Lai, Chung Hsin Lee, Cheng-Ming Tang, Huey-Shan Hung","doi":"10.1021/acsnanoscienceau.5c00004","DOIUrl":"10.1021/acsnanoscienceau.5c00004","url":null,"abstract":"<p><p>This study examined the effects of berberine, a bioactive alkaloid, on the apoptosis, proliferation, migration, and oxidative stress of DBTRG brain cancer cells and evaluated its potential when incorporated into a nanoparticle-mediated drug delivery system. DBTRG cells treated with 0.5, 1, 5, or 10 μg/mL of berberine for 48 h showed increased apoptosis through both intrinsic and extrinsic pathways, as evidenced by elevated annexin V+/propidium iodide- cells relative to untreated controls. Berberine effectively reduced cell proliferation by inducing cell cycle arrest at G1 and G2/M phases. It also inhibited cell migration by downregulating matrix metalloproteinases and modifying the cytoskeletal structure, and alleviated oxidative stress by enhancing antioxidant enzyme activity and lowering reactive oxygen species production. To overcome the limitations of berberine's low bioavailability, a nanoparticle-based delivery system was developed. The gold-collagen-berberine (Au-Col-BB) nanocarrier was characterized using UV-vis spectrophotometry, Fourier-transform infrared spectroscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. Au-Col-BB nanoparticles were engineered to enhance berberine's loading capacity and therapeutic efficacy. These nanoparticles entered DBTRG cells via endocytosis and progressed through the endosome-lysosome pathway, which significantly increased cellular uptake and therapeutic effectiveness. Annexin V/propidium iodide staining and cell cycle analysis demonstrated that Au-Col-BB nanoparticles promoted DBTRG cell apoptosis. The sub-G1 phase cell population increased by 19.4% (<i>p</i> < 0.001) compared to controls, while the S phase population decreased by 5.6% (<i>p</i> < 0.001), indicating enhanced apoptotic activity and reduced proliferation. In vivo analysis via retroorbital sinus injection of Au-Col-BB into BALB/c mice (<i>n</i> = 5) confirmed the nanoparticles' structural integrity and safety, as well as efficient accumulation in brain tissue. These findings underscore berberine's potential as an anticancer agent, particularly when delivered through a nanoparticle-based system to address the challenges of limited bioavailability and achieve targeted delivery to cancer cells.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"165-183"},"PeriodicalIF":4.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine’s Impact on Apoptosis, Proliferation, Uptake Efficiency, and Nanoparticle-Based Therapy in DBTRG Cells","authors":"Chiung-Chyi Shen,&nbsp;Meng-Yin Yang,&nbsp;Wan-Yu Hsieh,&nbsp;Gregory J. Tsay,&nbsp;Yi-Chin Yang,&nbsp;Yu-Fen Huang,&nbsp;Szu-Yuan Liu,&nbsp;Chih-Ming Lai,&nbsp;Chung Hsin Lee,&nbsp;Cheng-Ming Tang and Huey-Shan Hung*,&nbsp;","doi":"10.1021/acsnanoscienceau.5c0000410.1021/acsnanoscienceau.5c00004","DOIUrl":"https://doi.org/10.1021/acsnanoscienceau.5c00004https://doi.org/10.1021/acsnanoscienceau.5c00004","url":null,"abstract":"<p >This study examined the effects of berberine, a bioactive alkaloid, on the apoptosis, proliferation, migration, and oxidative stress of DBTRG brain cancer cells and evaluated its potential when incorporated into a nanoparticle-mediated drug delivery system. DBTRG cells treated with 0.5, 1, 5, or 10 μg/mL of berberine for 48 h showed increased apoptosis through both intrinsic and extrinsic pathways, as evidenced by elevated annexin V+/propidium iodide– cells relative to untreated controls. Berberine effectively reduced cell proliferation by inducing cell cycle arrest at G1 and G2/M phases. It also inhibited cell migration by downregulating matrix metalloproteinases and modifying the cytoskeletal structure, and alleviated oxidative stress by enhancing antioxidant enzyme activity and lowering reactive oxygen species production. To overcome the limitations of berberine’s low bioavailability, a nanoparticle-based delivery system was developed. The gold–collagen–berberine (Au–Col–BB) nanocarrier was characterized using UV–vis spectrophotometry, Fourier-transform infrared spectroscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. Au–Col–BB nanoparticles were engineered to enhance berberine’s loading capacity and therapeutic efficacy. These nanoparticles entered DBTRG cells via endocytosis and progressed through the endosome–lysosome pathway, which significantly increased cellular uptake and therapeutic effectiveness. Annexin V/propidium iodide staining and cell cycle analysis demonstrated that Au–Col–BB nanoparticles promoted DBTRG cell apoptosis. The sub-G1 phase cell population increased by 19.4% (<i>p</i> &lt; 0.001) compared to controls, while the S phase population decreased by 5.6% (<i>p</i> &lt; 0.001), indicating enhanced apoptotic activity and reduced proliferation. In vivo analysis via retroorbital sinus injection of Au–Col–BB into BALB/c mice (<i>n</i> = 5) confirmed the nanoparticles’ structural integrity and safety, as well as efficient accumulation in brain tissue. These findings underscore berberine’s potential as an anticancer agent, particularly when delivered through a nanoparticle-based system to address the challenges of limited bioavailability and achieve targeted delivery to cancer cells.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"165–183 165–183"},"PeriodicalIF":4.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsnanoscienceau.5c00004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical Guide to Automated TEM Image Analysis for Increased Accuracy and Precision in the Measurement of Particle Size and Morphology.","authors":"Kristen M Aviles, Benjamin J Lear","doi":"10.1021/acsnanoscienceau.4c00076","DOIUrl":"10.1021/acsnanoscienceau.4c00076","url":null,"abstract":"<p><p>A common desire in nanoscience is to describe the size and morphology of nanoparticles as observed from TEM images. Many times, this analysis is done manually, a lengthy process that is prone to errors and ambiguity in the measurements. While several research groups have reported excellent advances in machine-learned approaches to automated TEM image processing, the tools that they have developed often require specialized software or significant knowledge of coding. This state of affairs means that a majority of researchers in the field of nanoscience are not well-equipped to incorporate these advances into their normal workflows. In this tutorial, we describe how to use Weka segmentation within the free and open source program FIJI to automatically identify and characterize nanoparticles from TEM images. The approach we outline is not meant to discount the excellent results of groups working at the forefront of machine learning image analysis; rather, it is meant to bring similar tools to a broader audience by demonstrating how such processing can be done within the GUI-based interface of FIJIa program already commonly used within nanoscience research. We also discuss the advantages that arise from automatic processing of TEM images, including repeatability, time savings, the ability to process low-contrast images, and the additional types of characterization that can be performed following identification of particles. The overall goal is to provide an accessible tool that enables a more robust and repeatable analysis and descriptions of nanoparticles.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"5 3","pages":"117-127"},"PeriodicalIF":4.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}