ACS Physical Chemistry AuPub Date : 2024-05-27DOI: 10.1021/acsphyschemau.4c0001710.1021/acsphyschemau.4c00017
Devesh K. Pathak, Chanchal Rani, Aanchal Sati and Rajesh Kumar*,
{"title":"Developments in Raman Spectromicroscopy for Strengthening Materials and Natural Science Research: Shaping the Future of Physical Chemistry","authors":"Devesh K. Pathak, Chanchal Rani, Aanchal Sati and Rajesh Kumar*, ","doi":"10.1021/acsphyschemau.4c0001710.1021/acsphyschemau.4c00017","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00017https://doi.org/10.1021/acsphyschemau.4c00017","url":null,"abstract":"<p >Spectroscopic techniques, especially Raman spectroscopy, cover a large subset in the teaching and research domain of physical chemistry. Raman spectroscopy, and other Raman based techniques, establishes itself as a powerful analytical tool with diverse applications across scientific, industrial, and natural science (including biology and pharmacy) fields and helps in the progress of physical chemistry. Recent advancements and future prospects in Raman spectroscopy, focusing on key areas of innovation and potential directions for research and development, have been highlighted here along with some of the challenges that need to be addressed to prepare Raman based techniques for the future. Significant progress has been made in enhancing the sensitivity, spatial resolution, and time resolution of Raman spectroscopy techniques. Raman spectroscopy has applications in all areas of research but especially in biomedical applications, where Raman spectroscopy holds a great promise for noninvasive or minimally invasive diagnosis, tissue imaging, and drug monitoring. Improvements in instrumentation and laser technologies have enabled researchers to achieve higher sensitivity levels, investigate smaller sample areas with improved spatial resolution, and capture dynamic processes with high temporal resolution. These advancements have paved the way for a deeper understanding of molecular structure, chemical composition, and dynamic behavior in various materials and biological systems. It is high time that we consider whether Raman based techniques are ready to be improved based on the strength of the current era of AI/ML and quantum technology.</p>","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsphyschemau.4c00017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS Physical Chemistry AuPub Date : 2024-05-20DOI: 10.1021/acsphyschemau.4c0001110.1021/acsphyschemau.4c00011
Lan Hai Anh Tran, Lauren A. Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L. Amos and Anna Wang*,
{"title":"Measuring Vesicle Loading with Holographic Microscopy and Bulk Light Scattering","authors":"Lan Hai Anh Tran, Lauren A. Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L. Amos and Anna Wang*, ","doi":"10.1021/acsphyschemau.4c0001110.1021/acsphyschemau.4c00011","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00011https://doi.org/10.1021/acsphyschemau.4c00011","url":null,"abstract":"<p >We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modeling the vesicle’s scattering in a microscope with a Lorenz–Mie light scattering model and comparing the results to data holograms, we are able to measure the vesicle’s refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables the retrieval of vesicle loading for nanoscale vesicles.</p>","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsphyschemau.4c00011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141956680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lan Hai Anh Tran, Lauren A. Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L. Amos, Anna Wang
{"title":"Measuring Vesicle Loading with Holographic Microscopy and Bulk Light Scattering","authors":"Lan Hai Anh Tran, Lauren A. Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L. Amos, Anna Wang","doi":"10.1021/acsphyschemau.4c00011","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00011","url":null,"abstract":"We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modeling the vesicle’s scattering in a microscope with a Lorenz–Mie light scattering model and comparing the results to data holograms, we are able to measure the vesicle’s refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables the retrieval of vesicle loading for nanoscale vesicles.","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141146952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS Physical Chemistry AuPub Date : 2024-05-17DOI: 10.1021/acsphyschemau.4c0001810.1021/acsphyschemau.4c00018
Emily K. Oh, Timothy J. Krogmeier, Anthony W. Schlimgen and Kade Head-Marsden*,
{"title":"Singular Value Decomposition Quantum Algorithm for Quantum Biology","authors":"Emily K. Oh, Timothy J. Krogmeier, Anthony W. Schlimgen and Kade Head-Marsden*, ","doi":"10.1021/acsphyschemau.4c0001810.1021/acsphyschemau.4c00018","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00018https://doi.org/10.1021/acsphyschemau.4c00018","url":null,"abstract":"<p >There has been a recent interest in quantum algorithms for the modeling and prediction of nonunitary quantum dynamics using current quantum computers. The field of quantum biology is one area where these algorithms could prove to be useful as biological systems are generally intractable to treat in their complete form but amenable to an open quantum systems approach. Here, we present the application of a recently developed singular value decomposition (SVD) algorithm to two systems in quantum biology: excitonic energy transport through the Fenna–Matthews–Olson complex and the radical pair mechanism for avian navigation. We demonstrate that the SVD algorithm is capable of capturing accurate short- and long-time dynamics for these systems through implementation on a quantum simulator and conclude that while the implementation of this algorithm is beyond the reach of current quantum computers, it has the potential to be an effective tool for the future study of systems relevant to quantum biology.</p>","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsphyschemau.4c00018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141956674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emily K. Oh, Timothy J. Krogmeier, Anthony W. Schlimgen, Kade Head-Marsden
{"title":"Singular Value Decomposition Quantum Algorithm for Quantum Biology","authors":"Emily K. Oh, Timothy J. Krogmeier, Anthony W. Schlimgen, Kade Head-Marsden","doi":"10.1021/acsphyschemau.4c00018","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00018","url":null,"abstract":"There has been a recent interest in quantum algorithms for the modeling and prediction of nonunitary quantum dynamics using current quantum computers. The field of quantum biology is one area where these algorithms could prove to be useful as biological systems are generally intractable to treat in their complete form but amenable to an open quantum systems approach. Here, we present the application of a recently developed singular value decomposition (SVD) algorithm to two systems in quantum biology: excitonic energy transport through the Fenna–Matthews–Olson complex and the radical pair mechanism for avian navigation. We demonstrate that the SVD algorithm is capable of capturing accurate short- and long-time dynamics for these systems through implementation on a quantum simulator and conclude that while the implementation of this algorithm is beyond the reach of current quantum computers, it has the potential to be an effective tool for the future study of systems relevant to quantum biology.","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141059933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gunani Jayamaha, Levi Tegg, Cameron L. Bentley, Minkyung Kang
{"title":"High Throughput Correlative Electrochemistry-Microscopy Analysis on a Zn–Al Alloy","authors":"Gunani Jayamaha, Levi Tegg, Cameron L. Bentley, Minkyung Kang","doi":"10.1021/acsphyschemau.4c00016","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00016","url":null,"abstract":"Conventional electrodes and electrocatalysts possess complex compositional and structural motifs that impact their overall electrochemical activity. These motifs range from defects and crystal orientation on the electrode surface to layers and composites with other electrode components, such as binders. Therefore, it is vital to identify how these individual motifs alter the electrochemical activity of the electrode. Scanning electrochemical cell microscopy (SECCM) is a powerful tool that has been developed for investigating the electrochemical properties of complex structures. An example of a complex electrode surface is Zn–Al alloys, which are utilized in various sectors ranging from cathodic protection of steel to battery electrodes. Herein, voltammetric SECCM and correlative microstructure analysis are deployed to probe the electrochemical activities of a range of microstructural features, with 651 independent voltammetric measurements made in six distinctive areas on the surface of a Zn–Al alloy. Energy-dispersive X-ray spectroscopy (EDS) mapping reveals that specific phases of the alloy structure, particularly the α-phase Zn–Al, favor the early stages of metal dissolution (i.e., oxidation) and electrochemical reduction processes such as the oxygen reduction reaction (ORR) and redeposition of dissolved metal ions. A correlative analysis performed by comparing high-resolution quantitative elemental composition (i.e., EDS) with the corresponding spatially resolved cyclic voltammograms (i.e., SECCM) shows that the nanospot α-phase of the Zn–Al alloy contains high Al content (30–50%), which may facilitate local Al dissolution as the local pH increases during the ORR in unbuffered aqueous media. Overall, SECCM-based high-throughput electrochemical screening, combined with microstructure analysis, conclusively demonstrates that structure-composition heterogeneity significantly influences the local electrochemical activity on complex electrode surfaces. These insights are invaluable for the rational design of advanced electromaterials.","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140935570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS Physical Chemistry AuPub Date : 2024-05-10DOI: 10.1021/acsphyschemau.4c0001610.1021/acsphyschemau.4c00016
Gunani Jayamaha, Levi Tegg, Cameron L. Bentley and Minkyung Kang*,
{"title":"High Throughput Correlative Electrochemistry-Microscopy Analysis on a Zn–Al Alloy","authors":"Gunani Jayamaha, Levi Tegg, Cameron L. Bentley and Minkyung Kang*, ","doi":"10.1021/acsphyschemau.4c0001610.1021/acsphyschemau.4c00016","DOIUrl":"https://doi.org/10.1021/acsphyschemau.4c00016https://doi.org/10.1021/acsphyschemau.4c00016","url":null,"abstract":"<p >Conventional electrodes and electrocatalysts possess complex compositional and structural motifs that impact their overall electrochemical activity. These motifs range from defects and crystal orientation on the electrode surface to layers and composites with other electrode components, such as binders. Therefore, it is vital to identify how these individual motifs alter the electrochemical activity of the electrode. Scanning electrochemical cell microscopy (SECCM) is a powerful tool that has been developed for investigating the electrochemical properties of complex structures. An example of a complex electrode surface is Zn–Al alloys, which are utilized in various sectors ranging from cathodic protection of steel to battery electrodes. Herein, voltammetric SECCM and correlative microstructure analysis are deployed to probe the electrochemical activities of a range of microstructural features, with 651 independent voltammetric measurements made in six distinctive areas on the surface of a Zn–Al alloy. Energy-dispersive X-ray spectroscopy (EDS) mapping reveals that specific phases of the alloy structure, particularly the α-phase Zn–Al, favor the early stages of metal dissolution (i.e., oxidation) and electrochemical reduction processes such as the oxygen reduction reaction (ORR) and redeposition of dissolved metal ions. A correlative analysis performed by comparing high-resolution quantitative elemental composition (i.e., EDS) with the corresponding spatially resolved cyclic voltammograms (i.e., SECCM) shows that the nanospot α-phase of the Zn–Al alloy contains high Al content (30–50%), which may facilitate local Al dissolution as the local pH increases during the ORR in unbuffered aqueous media. Overall, SECCM-based high-throughput electrochemical screening, combined with microstructure analysis, conclusively demonstrates that structure-composition heterogeneity significantly influences the local electrochemical activity on complex electrode surfaces. These insights are invaluable for the rational design of advanced electromaterials.</p>","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsphyschemau.4c00016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ibrahim Dawod, Kajwal Patra, Sebastian Cardoch, H. Olof Jönsson, Jonas A. Sellberg, Andrew V. Martin, Jack Binns, Oscar Grånäs, Adrian P. Mancuso, Carl Caleman, Nicusor Timneanu
{"title":"Theoretical Studies of Anisotropic Melting of Ice Induced by Ultrafast Nonthermal Heating","authors":"Ibrahim Dawod, Kajwal Patra, Sebastian Cardoch, H. Olof Jönsson, Jonas A. Sellberg, Andrew V. Martin, Jack Binns, Oscar Grånäs, Adrian P. Mancuso, Carl Caleman, Nicusor Timneanu","doi":"10.1021/acsphyschemau.3c00072","DOIUrl":"https://doi.org/10.1021/acsphyschemau.3c00072","url":null,"abstract":"Water and ice are routinely studied with X-rays to reveal their diverse structures and anomalous properties. We employ a hybrid collisional-radiative/molecular-dynamics method to explore how femtosecond X-ray pulses interact with hexagonal ice. We find that ice makes a phase transition into a crystalline plasma where its initial structure is maintained up to tens of femtoseconds. The ultrafast melting process occurs anisotropically, where different geometric configurations of the structure melt on different time scales. The transient state and anisotropic melting of crystals can be captured by X-ray diffraction, which impacts any study of crystalline structures probed by femtosecond X-ray lasers.","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140935509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ACS Physical Chemistry AuPub Date : 2024-05-08DOI: 10.1021/acsphyschemau.3c0007210.1021/acsphyschemau.3c00072
Ibrahim Dawod*, Kajwal Patra, Sebastian Cardoch, H. Olof Jönsson, Jonas A. Sellberg, Andrew V. Martin, Jack Binns, Oscar Grånäs, Adrian P. Mancuso, Carl Caleman and Nicusor Timneanu*,
{"title":"Theoretical Studies of Anisotropic Melting of Ice Induced by Ultrafast Nonthermal Heating","authors":"Ibrahim Dawod*, Kajwal Patra, Sebastian Cardoch, H. Olof Jönsson, Jonas A. Sellberg, Andrew V. Martin, Jack Binns, Oscar Grånäs, Adrian P. Mancuso, Carl Caleman and Nicusor Timneanu*, ","doi":"10.1021/acsphyschemau.3c0007210.1021/acsphyschemau.3c00072","DOIUrl":"https://doi.org/10.1021/acsphyschemau.3c00072https://doi.org/10.1021/acsphyschemau.3c00072","url":null,"abstract":"<p >Water and ice are routinely studied with X-rays to reveal their diverse structures and anomalous properties. We employ a hybrid collisional-radiative/molecular-dynamics method to explore how femtosecond X-ray pulses interact with hexagonal ice. We find that ice makes a phase transition into a crystalline plasma where its initial structure is maintained up to tens of femtoseconds. The ultrafast melting process occurs anisotropically, where different geometric configurations of the structure melt on different time scales. The transient state and anisotropic melting of crystals can be captured by X-ray diffraction, which impacts any study of crystalline structures probed by femtosecond X-ray lasers.</p>","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsphyschemau.3c00072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141957203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dmitrii M. Nikolaev, Vladimir N. Mironov, Ekaterina M. Metelkina, Andrey A. Shtyrov, Andrey S. Mereshchenko, Nikita A. Demidov, Sergey Yu. Vyazmin, Tatiana B. Tennikova, Svetlana E. Moskalenko, Stanislav A. Bondarev, Galina A. Zhouravleva, Andrey V. Vasin, Maxim S. Panov, Mikhail N. Ryazantsev
{"title":"Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants","authors":"Dmitrii M. Nikolaev, Vladimir N. Mironov, Ekaterina M. Metelkina, Andrey A. Shtyrov, Andrey S. Mereshchenko, Nikita A. Demidov, Sergey Yu. Vyazmin, Tatiana B. Tennikova, Svetlana E. Moskalenko, Stanislav A. Bondarev, Galina A. Zhouravleva, Andrey V. Vasin, Maxim S. Panov, Mikhail N. Ryazantsev","doi":"10.1021/acsphyschemau.3c00073","DOIUrl":"https://doi.org/10.1021/acsphyschemau.3c00073","url":null,"abstract":"Genetically encoded voltage indicators (GEVIs) have found wide applications as molecular tools for visualization of changes in cell membrane potential. Among others, several classes of archaerhodopsin-3-based GEVIs have been developed and have proved themselves promising in various molecular imaging studies. To expand the application range for this type of GEVIs, new variants with absorption band maxima shifted toward the first biological window and enhanced fluorescence signal are required. Here, we integrate computational and experimental strategies to reveal structural factors that distinguish far-red bright archaerhodopsin-3-based GEVIs, Archers, obtained by directed evolution in a previous study (McIsaac et al., <i>PNAS,</i> 2014) and the wild-type archaerhodopsin-3 with an extremely dim fluorescence signal, aiming to use the obtained information in subsequent rational design. We found that the fluorescence can be enhanced by stabilization of a certain conformation of the protein, which, in turn, can be achieved by tuning the p<i>K</i><sub>a</sub> value of two titratable residues. These findings were supported further by introducing mutations into wild-type archeorhodopsin-3 and detecting the enhancement of the fluorescence signal. Finally, we came up with a rational design and proposed previously unknown Archers variants with red-shifted absorption bands (λ<sub>max</sub> up to 640 nm) and potential-dependent bright fluorescence (quantum yield up to 0.97%).","PeriodicalId":29796,"journal":{"name":"ACS Physical Chemistry Au","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140832055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}