Cell Reports Methods最新文献_第4页

SkinCom, a synthetic skin microbial community, enables reproducible investigations of the human skin microbiome. SkinCom 是一种合成皮肤微生物群落，能够对人类皮肤微生物群进行可重复的研究。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-06 DOI: 10.1016/j.crmeth.2024.100832

Asama Lekbua, Deepan Thiruppathy, Joanna Coker, Yuhan Weng, Fatemeh Askarian, Armin Kousha, Clarisse Marotz, Amber Hauw, Victor Nizet, Karsten Zengler

{"title":"SkinCom, a synthetic skin microbial community, enables reproducible investigations of the human skin microbiome.","authors":"Asama Lekbua, Deepan Thiruppathy, Joanna Coker, Yuhan Weng, Fatemeh Askarian, Armin Kousha, Clarisse Marotz, Amber Hauw, Victor Nizet, Karsten Zengler","doi":"10.1016/j.crmeth.2024.100832","DOIUrl":"10.1016/j.crmeth.2024.100832","url":null,"abstract":"Existing models of the human skin have aided our understanding of skin health and disease. However, they currently lack a microbial component, despite microbes' demonstrated connections to various skin diseases. Here, we present a robust, standardized model of the skin microbial community (SkinCom) to support in vitro and in vivo investigations. Our methods lead to the formation of an accurate, reproducible, and diverse community of aerobic and anaerobic bacteria. Subsequent testing of SkinCom on the dorsal skin of mice allowed for DNA and RNA recovery from both the applied SkinCom and the dorsal skin, highlighting its practicality for in vivo studies and -omics analyses. Furthermore, 66% of the responses to common cosmetic chemicals in vitro were in agreement with a human trial. Therefore, SkinCom represents a valuable, standardized tool for investigating microbe-metabolite interactions and facilitates the experimental design of in vivo studies targeting host-microbe relationships.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining spatial nonuniformities of all ipRGC types using an improved Opn4^cre recombinase mouse line. 利用改良的 Opn4cre 重组酶小鼠品系确定所有 ipRGC 类型的空间不均匀性。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-09 DOI: 10.1016/j.crmeth.2024.100837

Brannen Dyer, Sue O Yu, R Lane Brown, Richard A Lang, Shane P D'Souza

{"title":"Defining spatial nonuniformities of all ipRGC types using an improved Opn4cre recombinase mouse line.","authors":"Brannen Dyer, Sue O Yu, R Lane Brown, Richard A Lang, Shane P D'Souza","doi":"10.1016/j.crmeth.2024.100837","DOIUrl":"10.1016/j.crmeth.2024.100837","url":null,"abstract":"Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in several physiological light responses. In this study, we generate an improved Opn4cre knockin allele (Opn4cre(DSO)), which faithfully reproduces endogenous Opn4 expression and improves compatibility with widely used reporters. We evaluated the efficacy and sensitivity of Opn4cre(DSO) for labeling in retina and brain and provide an in-depth comparison with the extensively utilized Opn4cre(Saha) line. Through this characterization, Opn4cre(DSO) demonstrated higher specificity in labeling ipRGCs with minimal recombination escape. Leveraging a combination of electrophysiological, molecular, and morphological analyses, we confirmed its sensitivity in detecting all ipRGC types (M1-M6) and defined their unique topographical distribution across the retina. In the brain, the Opn4cre(DSO) line labels ipRGC projections with minimal labeling of cell bodies. Overall, the Opn4cre(DSO) mouse line represents an improved tool for studying ipRGC function and distribution, offering a means to selectively target these cells to study light-regulated behaviors and physiology.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a 3-dimensional organotypic model with characteristics of peripheral sensory nerves. 开发具有外周感觉神经特征的三维器官模型。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-07 DOI: 10.1016/j.crmeth.2024.100835

Madoka Koyanagi, Ryosuke Ogido, Akari Moriya, Mamiko Saigo, Satoshi Ihida, Tomoko Teranishi, Jiro Kawada, Tatsuya Katsuno, Kazuo Matsubara, Tomohiro Terada, Akira Yamashita, Satoshi Imai

{"title":"Development of a 3-dimensional organotypic model with characteristics of peripheral sensory nerves.","authors":"Madoka Koyanagi, Ryosuke Ogido, Akari Moriya, Mamiko Saigo, Satoshi Ihida, Tomoko Teranishi, Jiro Kawada, Tatsuya Katsuno, Kazuo Matsubara, Tomohiro Terada, Akira Yamashita, Satoshi Imai","doi":"10.1016/j.crmeth.2024.100835","DOIUrl":"10.1016/j.crmeth.2024.100835","url":null,"abstract":"We developed a rat dorsal root ganglion (DRG)-derived sensory nerve organotypic model by culturing DRG explants on an organoid culture device. With this method, a large number of organotypic cultures can be produced simultaneously with high reproducibility simply by seeding DRG explants derived from rat embryos. Unlike previous DRG explant models, this organotypic model consists of a ganglion and an axon bundle with myelinated A fibers, unmyelinated C fibers, and stereo-myelin-forming nodes of Ranvier. The model also exhibits Ca2+ signaling in cell bodies in response to application of chemical stimuli to nerve terminals. Further, axonal transection increases the activating transcription factor 3 mRNA level in ganglia. Axons and myelin are shown to regenerate 14 days following transection. Our sensory organotypic model enables analysis of neuronal excitability in response to pain stimuli and tracking of morphological changes in the axon bundle over weeks.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide and cell-type-selective profiling of in vivo small noncoding RNA:target RNA interactions by chimeric RNA sequencing. 通过嵌合 RNA 测序分析体内小非编码 RNA 与靶 RNA 相互作用的全基因组和细胞类型选择性概况。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-09 DOI: 10.1016/j.crmeth.2024.100836

Xinbei Li, William T Mills, Daniel S Jin, Mollie K Meffert

{"title":"Genome-wide and cell-type-selective profiling of in vivo small noncoding RNA:target RNA interactions by chimeric RNA sequencing.","authors":"Xinbei Li, William T Mills, Daniel S Jin, Mollie K Meffert","doi":"10.1016/j.crmeth.2024.100836","DOIUrl":"10.1016/j.crmeth.2024.100836","url":null,"abstract":"Small noncoding RNAs (sncRNAs) regulate biological processes by impacting post-transcriptional gene expression through repressing the translation and levels of targeted transcripts. Despite the clear biological importance of sncRNAs, approaches to unambiguously define genome-wide sncRNA:target RNA interactions remain challenging and not widely adopted. We present CIMERA-seq, a robust strategy incorporating covalent ligation of sncRNAs to their target RNAs within the RNA-induced silencing complex (RISC) and direct detection of in vivo interactions by sequencing of the resulting chimeric RNAs. Modifications are incorporated to increase the capacity for processing low-abundance samples and permit cell-type-selective profiling of sncRNA:target RNA interactions, as demonstrated in mouse brain cortex. CIMERA-seq represents a cohesive and optimized method for unambiguously characterizing the in vivo network of sncRNA:target RNA interactions in numerous biological contexts and even subcellular fractions. Genome-wide and cell-type-selective CIMERA-seq enhances researchers' ability to study gene regulation by sncRNAs in diverse model systems and tissue types.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precise detection of cell-type-specific domains in spatial transcriptomics. 在空间转录组学中精确检测细胞类型特异性结构域

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-09 DOI: 10.1016/j.crmeth.2024.100841

Zhihan Ruan, Weijun Zhou, Hong Liu, Jinmao Wei, Yichen Pan, Chaoyang Yan, Xiaoyi Wei, Wenting Xiang, Chengwei Yan, Shengquan Chen, Jian Liu

{"title":"Precise detection of cell-type-specific domains in spatial transcriptomics.","authors":"Zhihan Ruan, Weijun Zhou, Hong Liu, Jinmao Wei, Yichen Pan, Chaoyang Yan, Xiaoyi Wei, Wenting Xiang, Chengwei Yan, Shengquan Chen, Jian Liu","doi":"10.1016/j.crmeth.2024.100841","DOIUrl":"10.1016/j.crmeth.2024.100841","url":null,"abstract":"Cell-type-specific domains are the anatomical domains in spatially resolved transcriptome (SRT) tissues where particular cell types are enriched coincidentally. It is challenging to use existing computational methods to detect specific domains with low-proportion cell types, which are partly overlapped with or even inside other cell-type-specific domains. Here, we propose De-spot, which synthesizes segmentation and deconvolution as an ensemble to generate cell-type patterns, detect low-proportion cell-type-specific domains, and display these domains intuitively. Experimental evaluation showed that De-spot enabled us to discover the co-localizations between cancer-associated fibroblasts and immune-related cells that indicate potential tumor microenvironment (TME) domains in given slices, which were obscured by previous computational methods. We further elucidated the identified domains and found that Srgn may be a critical TME marker in SRT slices. By deciphering T cell-specific domains in breast cancer tissues, De-spot also revealed that the proportions of exhausted T cells were significantly increased in invasive vs. ductal carcinoma.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and generalization of tissue structures from spatial omics data. 从空间 omics 数据中发现和归纳组织结构。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-09 DOI: 10.1016/j.crmeth.2024.100838

Zhenqin Wu, Ayano Kondo, Monee McGrady, Ethan A G Baker, Benjamin Chidester, Eric Wu, Maha K Rahim, Nathan A Bracey, Vivek Charu, Raymond J Cho, Jeffrey B Cheng, Maryam Afkarian, James Zou, Aaron T Mayer, Alexandro E Trevino

{"title":"Discovery and generalization of tissue structures from spatial omics data.","authors":"Zhenqin Wu, Ayano Kondo, Monee McGrady, Ethan A G Baker, Benjamin Chidester, Eric Wu, Maha K Rahim, Nathan A Bracey, Vivek Charu, Raymond J Cho, Jeffrey B Cheng, Maryam Afkarian, James Zou, Aaron T Mayer, Alexandro E Trevino","doi":"10.1016/j.crmeth.2024.100838","DOIUrl":"10.1016/j.crmeth.2024.100838","url":null,"abstract":"Tissues are organized into anatomical and functional units at different scales. New technologies for high-dimensional molecular profiling in situ have enabled the characterization of structure-function relationships in increasing molecular detail. However, it remains a challenge to consistently identify key functional units across experiments, tissues, and disease contexts, a task that demands extensive manual annotation. Here, we present spatial cellular graph partitioning (SCGP), a flexible method for the unsupervised annotation of tissue structures. We further present a reference-query extension pipeline, SCGP-Extension, that generalizes reference tissue structure labels to previously unseen samples, performing data integration and tissue structure discovery. Our experiments demonstrate reliable, robust partitioning of spatial data in a wide variety of contexts and best-in-class accuracy in identifying expertly annotated structures. Downstream analysis on SCGP-identified tissue structures reveals disease-relevant insights regarding diabetic kidney disease, skin disorder, and neoplastic diseases, underscoring its potential to drive biological insight and discovery from spatial datasets.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. 利用 I-E 型 CRISPR-Cas3 在小鼠和大鼠子代中进行基因组编辑。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-08 DOI: 10.1016/j.crmeth.2024.100833

Kazuto Yoshimi, Akihiro Kuno, Yuko Yamauchi, Kosuke Hattori, Hiromi Taniguchi, Kouya Mikamo, Ryuya Iida, Saeko Ishida, Motohito Goto, Kohei Takeshita, Ryoji Ito, Riichi Takahashi, Satoru Takahashi, Tomoji Mashimo

{"title":"Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes.","authors":"Kazuto Yoshimi, Akihiro Kuno, Yuko Yamauchi, Kosuke Hattori, Hiromi Taniguchi, Kouya Mikamo, Ryuya Iida, Saeko Ishida, Motohito Goto, Kohei Takeshita, Ryoji Ito, Riichi Takahashi, Satoru Takahashi, Tomoji Mashimo","doi":"10.1016/j.crmeth.2024.100833","DOIUrl":"10.1016/j.crmeth.2024.100833","url":null,"abstract":"The type I CRISPR system has recently emerged as a promising tool, especially for large-scale genomic modification, but its application to generate model animals by editing zygotes had not been established. In this study, we demonstrate genome editing in zygotes using the type I-E CRISPR-Cas3 system, which efficiently generates deletions of several thousand base pairs at targeted loci in mice with 40%-70% editing efficiency without off-target mutations. To overcome the difficulties associated with detecting the variable deletions, we used a newly long-read sequencing-based multiplex genotyping approach. Demonstrating remarkable versatility, our Cas3-based technique was successfully extended to rats as well as mice, even by zygote electroporation methods. Knockin for SNP exchange and genomic replacement with a donor plasmid were also achieved in mice. This pioneering work with the type I CRISPR zygote editing system offers increased flexibility and broader applications in genetic engineering across different species.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nova-ST: Nano-patterned ultra-dense platform for spatial transcriptomics. Nova-ST：用于空间转录组学的纳米图案超密集平台。

IF 4.3

Cell Reports Methods Pub Date : 2024-08-19 Epub Date: 2024-08-06 DOI: 10.1016/j.crmeth.2024.100831

Suresh Poovathingal, Kristofer Davie, Lars E Borm, Roel Vandepoel, Nicolas Poulvellarie, Annelien Verfaillie, Nikky Corthout, Stein Aerts

引用次数: 0

Detection of fluorescent protein mechanical switching in cellulo. 检测细胞中的荧光蛋白机械开关。

IF 4.3

Cell Reports Methods Pub Date : 2024-07-15 Epub Date: 2024-07-09 DOI: 10.1016/j.crmeth.2024.100815

T Curtis Shoyer, Kasie L Collins, Trevor R Ham, Aaron T Blanchard, Juilee N Malavade, Benjamin A Johns, Jennifer L West, Brenton D Hoffman

{"title":"Detection of fluorescent protein mechanical switching in cellulo.","authors":"T Curtis Shoyer, Kasie L Collins, Trevor R Ham, Aaron T Blanchard, Juilee N Malavade, Benjamin A Johns, Jennifer L West, Brenton D Hoffman","doi":"10.1016/j.crmeth.2024.100815","DOIUrl":"10.1016/j.crmeth.2024.100815","url":null,"abstract":"The ability of cells to sense and respond to mechanical forces is critical in many physiological and pathological processes. However, determining the mechanisms by which forces affect protein function inside cells remains challenging. Motivated by in vitro demonstrations of fluorescent proteins (FPs) undergoing reversible mechanical switching of fluorescence, we investigated whether force-sensitive changes in FP function could be visualized in cells. Guided by a computational model of FP mechanical switching, we develop a formalism for its detection in Förster resonance energy transfer (FRET)-based biosensors and demonstrate its occurrence in cellulo within a synthetic actin crosslinker and the mechanical linker protein vinculin. We find that in cellulo mechanical switching is reversible and altered by manipulation of cell force generation, external stiffness, and force-sensitive bond dynamics of the biosensor. This work describes a framework for assessing FP mechanical stability and provides a means of probing force-sensitive protein function inside cells.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue. 抗体辅助选择性分离小鼠小脑组织中的浦肯野细胞核。

IF 4.3

Cell Reports Methods Pub Date : 2024-07-15 Epub Date: 2024-07-08 DOI: 10.1016/j.crmeth.2024.100816

Luke C Bartelt, Mouad Fakhri, Grazyna Adamek, Magdalena Trybus, Anna Samelak-Czajka, Paulina Jackowiak, Agnieszka Fiszer, Craig B Lowe, Albert R La Spada, Pawel M Switonski

{"title":"Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue.","authors":"Luke C Bartelt, Mouad Fakhri, Grazyna Adamek, Magdalena Trybus, Anna Samelak-Czajka, Paulina Jackowiak, Agnieszka Fiszer, Craig B Lowe, Albert R La Spada, Pawel M Switonski","doi":"10.1016/j.crmeth.2024.100816","DOIUrl":"10.1016/j.crmeth.2024.100816","url":null,"abstract":"We developed a method that utilizes fluorescent labeling of nuclear envelopes alongside cytometry sorting for the selective isolation of Purkinje cell (PC) nuclei. Beginning with SUN1 reporter mice, we GFP-tagged envelopes to confirm that PC nuclei could be accurately separated from other cell types. We then developed an antibody-based protocol to make PC nuclear isolation more robust and adaptable to cerebellar tissues of any genotypic background. Immunofluorescent labeling of the nuclear membrane protein RanBP2 enabled the isolation of PC nuclei from C57BL/6 cerebellum. By analyzing the expression of PC markers, nuclear size, and nucleoli number, we confirmed that our method delivers a pure fraction of PC nuclei. To demonstrate its applicability, we isolated PC nuclei from spinocerebellar ataxia type 7 (SCA7) mice and identified transcriptional changes in known and new disease-associated genes. Access to pure PC nuclei offers insights into PC biology and pathology, including the nature of selective neuronal vulnerability.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0