Cell Reports Methods最新文献

A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study. 一种基于多重pcr的测序方法，用于使用血浆样本诊断和区分棘球蚴病，一项概念验证研究。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-07 DOI: 10.1016/j.crmeth.2026.101428

Yanping Zhao, Yeqin Wang, Ming Zhi, Lin Yang, Wending Pang, Tian Chen, Yiyang Shi, Shu Shen, Hong-Bin Yan, Chunyang Li, Gengfu Wei, Yanyan Zhang, Xin Jin, Yan Zhang

{"title":"A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.","authors":"Yanping Zhao, Yeqin Wang, Ming Zhi, Lin Yang, Wending Pang, Tian Chen, Yiyang Shi, Shu Shen, Hong-Bin Yan, Chunyang Li, Gengfu Wei, Yanyan Zhang, Xin Jin, Yan Zhang","doi":"10.1016/j.crmeth.2026.101428","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101428","url":null,"abstract":"Echinococcosis poses a significant public health threat, and its diagnosis relies on imaging. Non-invasive plasma detection of Echinococcus cell-free DNA (cfDNA) offers a promising diagnostic approach. We developed a low-cost multiplex PCR panel with 230 primer pairs targeting Echinococcus mitochondrial genomes and nuclear DNA repeat regions. We validated the panel using gradient dilutions of two pathogen species' DNA and 81 plasma samples (53 patients and 28 controls), differentiating species through sequencing analysis. Our method detected Echinococcus genomic DNA at an input as low as 1 fg per reaction, and tests of clinical samples showed a sensitivity of 68.42% and specificity of 92.86%. Based on our detection, 80% of the clinically confirmed cases were correctly identified as positive echinococcosis cases, and cfDNA levels correlated significantly with lesion size. This study highlights the potential of targeted cfDNA sequencing for non-invasive echinococcosis diagnosis and species differentiation and offers a promising tool for doubtful cases.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101428"},"PeriodicalIF":4.5,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods. 含抗生素耐药基因的重组质粒对常见细胞培养处理方法的复原力。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-05 DOI: 10.1016/j.crmeth.2026.101430

Austin Gluth, Christian Zmasek, Stephen Chiu, Tae Seok Moon

{"title":"Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods.","authors":"Austin Gluth, Christian Zmasek, Stephen Chiu, Tae Seok Moon","doi":"10.1016/j.crmeth.2026.101430","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101430","url":null,"abstract":"Antibiotics have saved an untold number of people and animals since penicillin's miraculous discovery in 1928. In the following half-century, progressive discoveries involving antibiotic resistance genes (ARGs), the microorganisms responsible, and their transferrable genetic material have yielded the tools necessary for genetic engineering, birthing the biotechnologies that continue to revolutionize healthcare. After half a century of antibiotic use in the biological sciences, we are, however, faced with an inconvenient question: what happens to residual antibiotics and ARG-containing recombinant DNA after experiments? According to sequencing, we demonstrate that neither severe bleach treatments nor autoclaving completely destroys plasmid-encoded ARGs in bacterial cultures. Furthermore, we show that various bacteria can be transformed using the isolated DNA, confirming that intact plasmids survived these common cell culture disposal methods. This work will catalyze future policy discussions, the development of antibiotic-free selection systems, and continued support for research into the underexplored anthropogenic sources of engineered DNA.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101430"},"PeriodicalIF":4.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human cerebral organoids with microglia and vasculature model glioma stem cell interactions and radiotherapy response. 人脑类器官与小胶质细胞和血管模型胶质瘤干细胞相互作用和放疗反应。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-05 DOI: 10.1016/j.crmeth.2026.101425

Jérémy Raguin, Noa Legrand, Thierry Kortulewski, Oriane Bergiers, Christine Granotier-Beckers, Laure Chatrousse, Alexandra Benchoua, Laurent R Gauthier, François D Boussin, Marc-André Mouthon

{"title":"Human cerebral organoids with microglia and vasculature model glioma stem cell interactions and radiotherapy response.","authors":"Jérémy Raguin, Noa Legrand, Thierry Kortulewski, Oriane Bergiers, Christine Granotier-Beckers, Laure Chatrousse, Alexandra Benchoua, Laurent R Gauthier, François D Boussin, Marc-André Mouthon","doi":"10.1016/j.crmeth.2026.101425","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101425","url":null,"abstract":"Most human brain organoid models derived from induced pluripotent stem cells (iPSCs) lack vascular and/or immune components, despite their critical roles in maintaining brain homeostasis and contributing to pathophysiological processes. We established a method for generating vascularized complex cerebral organoids (CCOs) containing microglial cells (brain-resident macrophages) by incorporating bipotent hematopoietic/endothelial progenitors derived from the same iPSC lines. This approach led to the formation of extensive vascular-like structures with blood-brain barrier characteristics, which were perfused upon transplantation into immunodeficient mice. Additionally, microglial cells exhibiting typical phenotypes also developed within the CCOs. By co-culturing CCOs with glioma stem cells, we demonstrated that this model recapitulates the tumor niche of glioblastoma, showing vascular co-option, reprogramming of microglia into tumor-associated macrophages, and recurrence after radiotherapy. In conclusion, our vascularized, immunocompetent CCO model provides a platform to study brain development, glioma pathogenesis, and therapies.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101425"},"PeriodicalIF":4.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noninvasive focal gene transfer of chemogenetic proteins in the primate brain. 灵长类动物脑内化学发生蛋白的无创局灶基因转移。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-04 DOI: 10.1016/j.crmeth.2026.101427

Michael R Corigliano, T Vincenza Parks, Simeon S Guretse, Leila Letica, Isabela Zimmermann Rollin, Sarah K A Pell, Vinicius P Campos, Diego Szczupak, David J Schaeffer

{"title":"Noninvasive focal gene transfer of chemogenetic proteins in the primate brain.","authors":"Michael R Corigliano, T Vincenza Parks, Simeon S Guretse, Leila Letica, Isabela Zimmermann Rollin, Sarah K A Pell, Vinicius P Campos, Diego Szczupak, David J Schaeffer","doi":"10.1016/j.crmeth.2026.101427","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101427","url":null,"abstract":"We leverage our recent development of transcranial focused ultrasound (tFUS) for noninvasive, focal delivery of adeno-associated viruses (AAVs)-encoding excitatory designer receptors exclusively activated by designer drugs (DREADDs)-to marmoset frontal cortex. Using FDG positron emission tomography, we demonstrate significant increases in glucose metabolism at the site of viral delivery after administering the DREADD-specific agonist deschloroclozapine (DCZ), as compared to vehicle control. Focal neuronal DREADD expression was confirmed by immunohistochemistry at the site of opening. Through a comparison of awake resting-state functional connectivity and structural connectivity from the sites of delivery, we demonstrate that increases in glucose metabolism occur within both mono- and polysynaptically connected brain regions. Taken together, these results demonstrate the ability to focally deliver excitatory chemogenetics without the need for surgery, allowing for activation of long-range frontal cortex circuits of the primate brain.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101427"},"PeriodicalIF":4.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DRIFT-EM enables direct wafer retrieval of ultrathin serial sections for large-volume electron microscopy. DRIFT-EM可以直接对超薄连续切片进行大容量电子显微镜的晶圆检索。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-04 DOI: 10.1016/j.crmeth.2026.101429

Nelson Medina, Joseph V Gogola, Kevin Boergens, Fuming Yang, Yaron Meirovitch, Jeff W Lichtman, Narayanan Kasthuri, Gregg Wildenberg

{"title":"DRIFT-EM enables direct wafer retrieval of ultrathin serial sections for large-volume electron microscopy.","authors":"Nelson Medina, Joseph V Gogola, Kevin Boergens, Fuming Yang, Yaron Meirovitch, Jeff W Lichtman, Narayanan Kasthuri, Gregg Wildenberg","doi":"10.1016/j.crmeth.2026.101429","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101429","url":null,"abstract":"Large-volume serial electron microscopy (vEM) has transformed neuroscience by enabling reconstructions of neural circuits at synaptic resolution. Approaches to collecting libraries of ultrathin sections required for producing vEM datasets have been devised (e.g., automated tape-collecting ultramicrotome [ATUM], MagC, and GAUSS-EM), which has advanced the scalability of vEM in distinct ways, yet widespread adoption remains constrained by cost, hardware requirements, or infrastructure demands. Here, we introduce direct retrieval by ionizer-facilitated transfer for electron microscopy (DRIFT-EM), a low-cost and easily implemented platform designed to broaden access to direct wafer-based collection of ultrathin sections. Off-the-shelf static ionizers clear newly cut sections from the knife edge, a thermoplastic boundary confines sections, and an open-source software pipeline automates region of interest identification to guide imaging. DRIFT-EM achieves section packing densities comparable to established magnetic methods, with minimal setup cost and maintenance. Its modular style, 3D-printed components, and open design facilitate integration with other vEM workflows, helping lower barriers to creating connectomes.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101429"},"PeriodicalIF":4.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-precision stress prediction using deep energy network enhanced by stress equilibrium with Delaunay integration on refined grid. 精细化网格上Delaunay积分强化应力平衡的深部能量网络高精度应力预测。

IF 4.5

Cell Reports Methods Pub Date : 2026-05-04 DOI: 10.1016/j.crmeth.2026.101424

Yongqiang Ye, Dongming An, Hui Li, Wenshuai Wang, Xing Li, Pengpeng Shi

{"title":"High-precision stress prediction using deep energy network enhanced by stress equilibrium with Delaunay integration on refined grid.","authors":"Yongqiang Ye, Dongming An, Hui Li, Wenshuai Wang, Xing Li, Pengpeng Shi","doi":"10.1016/j.crmeth.2026.101424","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101424","url":null,"abstract":"We present the deep energy method enhanced by stress equilibrium (DEM-SE), a physics-informed neural network (PINN) architecture for high-precision prediction of complex stress fields in elastic plates. The method calculates total potential energy via Delaunay integration on a locally refined triangular grid and enforces stress-equilibrium equations as physical constraints to construct a hybrid loss function. Its accuracy in stress concentration prediction and ability to characterize complex stresses in nonhomogeneous materials are validated through four typical elastic deformation cases. Using sparse displacement measurements from just 200 local nodes, combined with equilibrium-based constraints, the PINN accurately reconstructs full-field displacements, outperforms purely data-driven methods, and enables simultaneous inversion of nonhomogeneous elastic modulus distributions with constant Poisson's ratio. Ablation studies clarify the contributions of grid refinement, Delaunay integration, and the equilibrium constraint. This work establishes DEM-SE as a robust tool for stress analysis and offers insights for designing PINNs in elasticity applications.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101424"},"PeriodicalIF":4.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inferring cell-specific gene regulatory networks based on causal graph embedding. 基于因果图嵌入的细胞特异性基因调控网络推断。

IF 4.5

Cell Reports Methods Pub Date : 2026-04-29 DOI: 10.1016/j.crmeth.2026.101423

Guojie Li, Rui Qiao, Yunnuo Xu, Feng Jiao, Peiluan Li, Luonan Chen

{"title":"Inferring cell-specific gene regulatory networks based on causal graph embedding.","authors":"Guojie Li, Rui Qiao, Yunnuo Xu, Feng Jiao, Peiluan Li, Luonan Chen","doi":"10.1016/j.crmeth.2026.101423","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101423","url":null,"abstract":"Gene regulatory networks (GRNs) at single-cell resolution provide a fundamental framework for understanding cellular functions and regulatory mechanisms. However, existing methods often focus on regulatory relationships among genes while overlooking intercellular heterogeneity and global expression organization across cell populations. Here, we present CSGRN, a supervised computational framework that integrates graph embedding and conditional cell-specific networks (CCSNs) to infer GRNs for individual cells from single-cell RNA sequencing (scRNA-seq) data. By incorporating causal regulatory structures and integrating local and global representations, CSGRN improves the accuracy and robustness of regulatory network inference. Benchmark analyses across three datasets demonstrated that CSGRN outperforms nine existing approaches. In addition, we developed two downstream analytical strategies-signal flow analysis and gene perturbation simulation-to quantify regulatory relationships and explore regulatory dynamics. These analyses reveal cell type-specific regulatory programs and key regulators involved in cellular differentiation and disease-related processes, providing a framework for investigating gene regulation in complex biological systems.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101423"},"PeriodicalIF":4.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated multi-dimensional modeling of non-model bacteria identifies engineering targets for acarbose biosynthesis optimization. 非模式细菌的综合多维建模确定了阿卡波糖生物合成优化的工程靶点。

IF 4.5

Cell Reports Methods Pub Date : 2026-04-28 DOI: 10.1016/j.crmeth.2026.101426

Feifei Cai, Shijie Zhang, Yang Dai, Ziqi Zhao, Xinnan Fu, Qianjin Kang, Yongyong Shi, Zhuo Wang, Linquan Bai

{"title":"Integrated multi-dimensional modeling of non-model bacteria identifies engineering targets for acarbose biosynthesis optimization.","authors":"Feifei Cai, Shijie Zhang, Yang Dai, Ziqi Zhao, Xinnan Fu, Qianjin Kang, Yongyong Shi, Zhuo Wang, Linquan Bai","doi":"10.1016/j.crmeth.2026.101426","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101426","url":null,"abstract":"Metabolic engineering for high-value compounds such as acarbose, a diabetes drug, requires systematic understanding of metabolic regulation. Here, we applied a multi-dimensional systems biology approach in Actinoplanes sp. SE50/110, a non-model acarbose-producing bacterium. We reconstructed an improved genome-scale metabolic model (iASE1267) with expanded metabolic coverage and a MEMOTE score of 80%, enabling more accurate phenotype predictions. Using a dual-objective OptRAM strain design strategy, we identified two sets of static engineering targets, including AcbR overexpression with adenylosuccinate lyase repression, and overexpression of dTDP-glucose 4,6-dehydratase with repression of 4-(cytidine 5'-diphospho)-2-methyl-D-erythritol kinase. Time-course metabolic modeling further revealed dynamic metabolic valves-ASPO1, PC, and PYK-governing flux redistribution. Integrating these targets, we reconstructed a core transcription-metabolism network and identified two pleiotropic negative transcription factors (TFs). Experimental validation of these TFs and metabolic genes increased acarbose titers by 18%-23%. This work establishes a framework integrating static/dynamic metabolic modeling with transcriptional networks for engineering non-model microbes.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101426"},"PeriodicalIF":4.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EthoPy provides an accessible platform for reproducible behavioral neuroscience. EthoPy为重复性行为神经科学提供了一个可访问的平台。

IF 4.5

Cell Reports Methods Pub Date : 2026-04-23 DOI: 10.1016/j.crmeth.2026.101421

Alexandros Evangelou, Maria Diamantaki, Konstantina Georgelou, Zoi Drakaki, Lydia Ntanavara, Gerasimos Gerardos, Sofia Morou, Nikolaos Chatziris, Zoi Dogani, Elissavet Anna Petsalaki, Odysseas Nikolaos Raos, Anastasios Gratsakis, Stamatios Aliprantis, Athanasia Papoutsi, Emmanouil Froudarakis

{"title":"EthoPy provides an accessible platform for reproducible behavioral neuroscience.","authors":"Alexandros Evangelou, Maria Diamantaki, Konstantina Georgelou, Zoi Drakaki, Lydia Ntanavara, Gerasimos Gerardos, Sofia Morou, Nikolaos Chatziris, Zoi Dogani, Elissavet Anna Petsalaki, Odysseas Nikolaos Raos, Anastasios Gratsakis, Stamatios Aliprantis, Athanasia Papoutsi, Emmanouil Froudarakis","doi":"10.1016/j.crmeth.2026.101421","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101421","url":null,"abstract":"As brain activity is tightly coupled to behavior, an accurate understanding of neural functions necessitates consideration of behavioral tasks that capture the complexity and variety that animals encounter. Nevertheless, animal training for behavioral experiments is often labor intensive, costly, and difficult to standardize. To overcome these challenges, we developed EthoPy, an open-source, Python-based behavioral control framework that integrates stimulus presentation, hardware management, and data logging. EthoPy supports diverse behavioral paradigms, stimulus modalities, and experimental systems, from home cage to head-fixed configurations, while operating on affordable hardware, such as Raspberry Pi. Its modular architecture and database integration enable scalable, high-throughput automatic behavioral training with minimal experimenter involvement while ensuring reproducibility through comprehensive metadata tracking. By automating training workflows, EthoPy makes it feasible to implement sophisticated behavioral paradigms that are traditionally difficult to achieve. EthoPy, thus, provides an accessible, extensible framework to study behavior and the underlying neural activity.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101421"},"PeriodicalIF":4.5,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput histopathology for complex in vitro models. 复杂体外模型的高通量组织病理学。

IF 4.5

Cell Reports Methods Pub Date : 2026-04-23 DOI: 10.1016/j.crmeth.2026.101419

Marius F Harter, Elisa D'Arcangelo, Julien Aubert, Barbora Lavickova, Charles Havnar, Bilgenaz Stoll, Irineja Cubela, Adrian M Filip, Laura Gaspa-Toneu, Julian Scherer, Evdoxia Karagkiozi, Jean-Samuel Dupré, Rubén López-Sandoval, Rebecca Hsia, Leah M Norona, Giovanna Brancati, Ryo Okuda, J Gray Camp, Eliah R Shamir, Jose Luis Garcia-Cordero, Iago Pereiro, Nikolche Gjorevski

{"title":"High-throughput histopathology for complex in vitro models.","authors":"Marius F Harter, Elisa D'Arcangelo, Julien Aubert, Barbora Lavickova, Charles Havnar, Bilgenaz Stoll, Irineja Cubela, Adrian M Filip, Laura Gaspa-Toneu, Julian Scherer, Evdoxia Karagkiozi, Jean-Samuel Dupré, Rubén López-Sandoval, Rebecca Hsia, Leah M Norona, Giovanna Brancati, Ryo Okuda, J Gray Camp, Eliah R Shamir, Jose Luis Garcia-Cordero, Iago Pereiro, Nikolche Gjorevski","doi":"10.1016/j.crmeth.2026.101419","DOIUrl":"https://doi.org/10.1016/j.crmeth.2026.101419","url":null,"abstract":"Histology has been a cornerstone for complex in vitro model (CIVM) characterization for decades. However, it remains a low-throughput method with time-consuming workflows. Here, we introduce a holistic \"histo-workflow,\" utilizing 3D-printed histomolds that facilitate co-planar embedding of CIVMs at high throughput, resulting in up to 80 samples in one section. We developed a variety of model-specific histomold designs that enable spatially controlled histological sectioning and downstream analyses. We describe these workflows, including mold generation, high-plex staining, and image analysis, and exemplify their application to histological analyses of various CIVMs. Altogether, the histomolds introduced here afford opportunities for CIVM processing and analysis, while significantly reducing labor and reagent resources, thereby democratizing high-throughput handling of CIVMs in histopathology.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101419"},"PeriodicalIF":4.5,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0