Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry最新文献_第9页

The T4/TBG ratio in healthy subjects and in patients with disorders of thyroid function: correlation with FT4 concentrations 健康受试者和甲状腺功能紊乱患者的T4/TBG比值:与FT4浓度的相关性

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMB0504241P

I. Petrovic, S. Savin, S. Spasić, D. Cvejić, N. Paunković, J. Paunković

{"title":"The T4/TBG ratio in healthy subjects and in patients with disorders of thyroid function: correlation with FT4 concentrations","authors":"I. Petrovic, S. Savin, S. Spasić, D. Cvejić, N. Paunković, J. Paunković","doi":"10.2298/JMB0504241P","DOIUrl":"https://doi.org/10.2298/JMB0504241P","url":null,"abstract":"Summary: The proportion of free hormones can be estimated in the presence of protein-bound hormones by a variety of direct assay approaches or by a two test strategy to calculate a free hormone »index«. The T4/TBG ratio, determined from measurements of total thyroxine (TT4) and thyroxine-binding globulin (TBG) concentrations, is an appropriate index with improved diagnostic accuracy compared with T4. In this study, we calculated the T4/TBG ratio after determining serum TT4 and TBG by radioimmunoassay (INEP, SCG) in euthyroid persons (n=102), in patients with hypothyrosis (n=33) and hyperthyrosis (n=66), as well as in healthy pregnant women (n=346). The reference range for each group of patients was determined. Values obtained for the T4/TBG ratio in healthy subjects varied from 2.1 to 4.4. They were lower in hypothyrosis (0.5 ’2.2) and elevated in hyperthyrosis (4.7–13.1). The reference intervals of the T4/TBG ratio were also determined for each trimester of pregnancy. A significant correlation was obtained between the values for T4/TBG ratio and FT4 concentration, determined by the two-step radioimmunoassay (CIS-bio international, France), in healthy subjects and patients with thyroid disorders (r=0.93), as well as during pregnancy (0.59). In conclusion, we determined reference intervals for the T4/TBG ratio, confirming its practical usefulness in the assessment of thyroid function.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132574787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TARTARATE-RESISTANT ACID PHOSPHATASE, OSTEOCALCIN AND N-TERMINAL PEPTIDE OF PROCOLLAGEN IN THE DIAGNOSTICS OF OSTEOPOROSIS 抗酒石酸酸酸性磷酸酶、骨钙素和前胶原n端肽在骨质疏松症诊断中的意义

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMB0603249L

J. Laloš-Miljuš, J. Mehanović-Nikolić, A. Jakovljević

引用次数: 3

Interaction of intracellular loops of dopamine d1 receptor with g protein subunits 多巴胺d1受体胞内环与g蛋白亚基的相互作用

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0203255D

Sergej Djuranovic, Jelena Predic, M. Backovic, V. Soskic

{"title":"Interaction of intracellular loops of dopamine d1 receptor with g protein subunits","authors":"Sergej Djuranovic, Jelena Predic, M. Backovic, V. Soskic","doi":"10.2298/JMH0203255D","DOIUrl":"https://doi.org/10.2298/JMH0203255D","url":null,"abstract":"Summary: A simple and rapid method for qualitative and quantitative estimation of Ga subunit interactions with the second and the third intracellular loop, as well as with C-terminal part of human D1 dopamine receptor has been developed. For this purpose, D1-ICL2 and D1-ICL3 were cloned in pGEX-2T vector and expressed in E. coli BL21 as fusion proteins with glutathione-S-transferase (D1-ICL2-GST and D1-ICL3-GST). C-terminal part was cleaved into two fragments which were cloned in pGEX-2T and expressed in E. coli BL21 DE3 as fusion proteins with glutathione-S-transferase (D1-CTSF-GST and D1-CTLF-GST). The resulting soluble constructs were purified by affinity chromatography on glutathione-Sepharose. Ga subunits were expressed and purified as His-tagged proteins (Gao and Gai1 in E.coli BL21 DE3 and Gas in E. coli JM 109). For quantitative assay, varying concentrations of pure His-tagged Ga subunits were immobilized on His-Bind resin and titrated with fusion proteins and the interactions were estimated by a colorimetric assay for GST activity determination. Similar assay was employed to qualitatively demonstrate the interactions. For this purpose pure fusion proteins were immobilized on glutathione-Sepharose in known concentrations and treated with known concentrations of pure His-tagged Ga subunits. Thus created complexes were eluted from glutathione-Sepharose and analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It was shown that D1-CTSF interacts specifically with Gas subunit, and D1CTLF with Gao. No other interactions were observed. Based on saturation binding analyses, Kd values in nanomolar range of concentrations demonstrated the highest binding affinity of His-Gas for D1-CTSF-GST and of His-Gao for D1-CTLF-GST.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114734127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uloga prostatičnog specifičnog antigena u ranoj dijagnozi karcinoma prostate

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0304283L

N. Lalić, M. Acimovic, N. Majkić-Singh, R. Micić

{"title":"Uloga prostatičnog specifičnog antigena u ranoj dijagnozi karcinoma prostate","authors":"N. Lalić, M. Acimovic, N. Majkić-Singh, R. Micić","doi":"10.2298/JMH0304283L","DOIUrl":"https://doi.org/10.2298/JMH0304283L","url":null,"abstract":"Prostaticni specificni antigen (PSA) spada u najznacajnije tumorske markere koji se koriste u ranom otkrivanju karcinoma prostate, određivanju stadijuma bolesti, kao i pracenju ucinka terapije kod muskaraca sa karcinomom prostate. Standardizacija metoda za određivanje PSA komplikuje se pojavom PSA u serumu u obliku vise izoformi od kojih su najznacajnije forme slobodnog PSA, PSA vezanog za alfa-1-antihimotripsin, koje imaju antigene determinante za koje se vezuju razlicita antitela, kao i PSA vezan za alfa-2-makroglobulin, koji nema antigene determinante, tako da se ova izoforma PSA ne može odrediti pomocu imunoloskih postupaka. Postoji veliki broj imunoloskih metoda za određivanje PSA, koje se međusobno razlikuju po antitelima koja se koriste, po obeleživacu, standardima i po vrednostima PSA koje se dobijaju. Da bi se dizajnirao dobar imunoloski test neophodno je standardizovati kalibratore i odabrati odgovarajuca antitela, pri cemu se mora voditi racuna o ostalim karakteristikama testa kao npr. osobini cvrste faze, kinetici reakcije itd. Posto se nalazi u adenomatoznom, normalnom i kancerogenom tkivu prostate, dijagnosticki znacaj PSA je ogranicen usled preklapanja rezultata kod pacijenata sa karcinomom i benignom hiperplazijom prostate (BPH). Ovo je narocito izraženo za vrednosti PSA koje se nalaze u tzv. \"sivoj zoni\", i to između 4,0 i 10,0 ng/mL PSA. Da bi se povecala specificnost za dijagnostiku karcirioma prostate primenjuju se derivati PSA kao sto su: gustina PSA (PSA density) dinamika rasta PSA (PSA velocity), PSA vrednosti korigovane godinama starosti, određivanje slobodnog (sl PSA) i kompleksiranog molekula PSA (cPSA) i njihovog odnosa sa ukupnim PSA.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131953110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

General requirements for the competence of testing laboratories and their implementation 测试实验室能力的一般要求及其实施

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0204313N

Tatjana Nesic, Radomir Bošković

引用次数: 0

Protection management of working in health laboratories 卫生实验室工作人员防护管理

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0204317I

M. Ilić, A. Postic-grujin, N. Majkić-Singh

引用次数: 0

Implementation of a two-dimensional barcode in the preanalytic phase 二维条码在预分析阶段的实现

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0403317M

C. Müller, J. Singer

引用次数: 1

Haemostasis and biochemical parameters of liver function monitoring in the surgical treatment of portal hypertension 门静脉高压症手术治疗中的止血及肝功能生化指标监测

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0501055V

N. Vavić, B. Radević, B. Brkić

引用次数: 0

Apolipoprotein e gene polymorphism as a risk factor for ischemic cerebrovascular disease 载脂蛋白e基因多态性是缺血性脑血管病的危险因素

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0403255S

S. Stankovic, Zagorka Jovanovic-Markovic, N. Majkić-Singh, D. Stankovic, S. Glišić, D. Zivkovic, S. Kostic, N. D. Alavantic

引用次数: 7

HYPERPROLACTINAEMIA AS A POTENTIAL CAUSE OF INFERTILITY 高催乳素血症是不孕的潜在原因

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0304335D

S. Dragojević, M. Vasiljević, A. Mitrović, R. Dunjić, S. Dikić, F. Canović

引用次数: 0