2011 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第7页

MEMS components with perfectly protected edges and corners in Si{110} wafers 在Si{110}晶圆中具有完美保护边缘和角落的MEMS元件

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102158

P. Pal, Kazuo Sato, H. Hida

引用次数: 0

Microfluidics and microfabrication technology for highly precise cell manipulation and cultivation 用于高精度细胞操作和培养的微流体和微加工技术

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102178

M. Yamada, M. Seki

{"title":"Microfluidics and microfabrication technology for highly precise cell manipulation and cultivation","authors":"M. Yamada, M. Seki","doi":"10.1109/MHS.2011.6102178","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102178","url":null,"abstract":"Microfluidic processes are essential techniques not only for manipulating micrometer-size objects like cells, particles, and biomacromolecules, but also for producing micrometer-size objects with highly-controlled morphologies or compositions. First, continuous and rapid separation and accumulation methods for particles or cells using microfluidic devices are introduced. Microfluidic devices have a potential to facilitate rapid and precise particle manipulation, due to accurately fabricated structures close to particle sizes, in micrometer or dimensions. Newly developed methods enable a size- and/or shape-dependent, precise separation of biological cells or soft matters. Next, we present microfluidic devices for preparing functional micrometer-size hydrogel materials having fibrous or particulate morphology. The physical/chemical heterogeneity of the prepared materials allows the incorporated cells to grow differently from the conventional plate cultivation, which is useful for preparing unit structures mimicking the in-vivo tissues. In addition to these materials, here we introduce recently developed several microfluidic/microfabrication techniques, including the preparation processes of microstructured and layered hydrogel plates, micropatterning of ultra-thin hydrogels utilizing local surface modification, micronozzle structures for producing actuating lipid vesicles, and the continuous microfluidic cell processing. These techniques would be useful for rapidly fabricating relatively-large tissue models by assembling the unit materials and/or by employing various conventional/unconventional micromanipulation technologies.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126564473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modification of activity pattern induced by synaptic enhancements in a semi-artificial network of living neurons 半人工活神经元网络中突触增强诱导的活动模式改变

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102187

Masaaki Murata, Hidekatsu Ito, Teppei Taenaka, S. Kudoh

{"title":"Modification of activity pattern induced by synaptic enhancements in a semi-artificial network of living neurons","authors":"Masaaki Murata, Hidekatsu Ito, Teppei Taenaka, S. Kudoh","doi":"10.1109/MHS.2011.6102187","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102187","url":null,"abstract":"Higher brain function such as memory formation was not performed by activity of a single neuron but performed by functions of a complex network of neuronal cells. The simple small-scaled network of neuronal cells is fully suitable for such interactions between neurons. Dissociated neurons form a network depending on their electrical activity and spontaneous activity frequently observed within a week. We cultured a network of dissociated neurons on a culture dish with 64 planer microelectrodes. We induced synaptic enhancement in cultured neuronal networks by exposing to Mg2+-free condition for 20 min. Mg2+-free condition was achieved by exchanging of normal cell external solution to Mg2+-free recording solution. After the induction of synaptic enhancement, we analyzed activity pattern by an autocorrelogram-based and crosscorrelogram-based method. Autocorrelogram of the neuronal activity centralized, suggesting that the accuracy of the periodicity increased. This drastic change was induced within only 20 minutes. Crosscorrelogram shows those network activity changed to be more synchronously than one before exposure to Mg2+-free condition. These results suggest that functional connections in a semi-artificial neuronal network were changed to ones performing enhanced network activity than before. The modification of the spatiotemporal pattern of activity is thought to be a base of memory in vivo experiments. We performed similar phenomenon in this semi-artificial, autonomously reorganized network of neurons. By elucidation of these modified functional connections in neural network, we can find a cue how to control biological memory formation.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"123 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133596708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High sensitivity vasculature models and catheter trajectory reconstruction using a bi-planar vision system 使用双平面视觉系统的高灵敏度血管模型和导管轨迹重建

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102198

C. Tercero, S. Ikeda, T. Fukuda, F. Arai, M. Negoro, I. Takahashi

{"title":"High sensitivity vasculature models and catheter trajectory reconstruction using a bi-planar vision system","authors":"C. Tercero, S. Ikeda, T. Fukuda, F. Arai, M. Negoro, I. Takahashi","doi":"10.1109/MHS.2011.6102198","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102198","url":null,"abstract":"Simulator based evaluation has been used for medical licensing, intravascular tools evaluation and robotic catheter insertion systems research. However it is need to develop quantitative evaluation methods for the catheter trajectory inside vasculature phantoms. In this study we present two technologies developed for that purpose. First we present models of vasculature made of epoxy resin with higher sensitivity for photoelastic effect than previously used modeling materials. The second contribution for that purpose is a stereovision system for measuring evaluation parameters such as catheter tip motion capture, model deformation and stress within the model at 3fps enabling comparison catheter trajectories and catheter insertion robot control. Models of carotid artery, saccular aneurysm with bleb, aorta arch and giant aneurysm were built using the epoxy resin. And for the robot control using the vision system, from 10 consecutive trials of trajectory reconstruction in a model of carotid artery a success rate of 80% was obtained with feedback control activated.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134110293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Estimation of tongue movement based on suprahyoid muscle activity 舌骨上肌活动对舌运动的估计

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102222

M. Sasaki, T. Arakawa, Atsushi Nakayama, G. Obinata, M. Yamaguchi

引用次数: 11

Fabrication of thermoresponsive surface for cell sheet harvest by photopolymerization 用光聚合法制备用于收获细胞片的热响应表面

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102179

K. Itoga, J. Kobayashi, M. Yamato, T. Okano

{"title":"Fabrication of thermoresponsive surface for cell sheet harvest by photopolymerization","authors":"K. Itoga, J. Kobayashi, M. Yamato, T. Okano","doi":"10.1109/MHS.2011.6102179","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102179","url":null,"abstract":"Our laboratory developed “cell sheet engineering”, which has been applied to regenerative medicine. However, the applications are restricted to the transplants of single-, double-, and triple-layered cell sheets, because of the poor supplies of nutrients and oxygen. To fabricate thicker and dense tissues in vitro, multi-layered cell sheets require to have capillary networks. Additionally, individual tissues found in animal body have individual specific micropatterns of cells for expressing their specific functions and characters. Therefore, we focus on micropatterned co-cultured cell sheets as one of the methods to fabricate thicker and more functional tissues. Polymerization of thermoresponsive polymer, poly(N-isopropylacrylamide) (PIPAAm), by electron beam irradiation has been used for fabrication of thermoresponsive surfaces on tissue culture polystyrene dishes. However, it is difficult to fabricate micropatterned surfaces of thermoresponsive polymer by electron beam irradiation. Therefore, we attempted to optimize photopolymerization condition of PIPAAm with a visible light photopolymerization initiator, water-soluble camphorquinone, as the fabrication method of thermoresponsive surface.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"84 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113987105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotool exchanger system using low-melting metal under environmental SEM 环境扫描电镜下低熔点金属纳米刀具交换系统

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102161

M. Nakajima, T. Kawamoto, M. Kojima, T. Fukuda

引用次数: 1

Grasping strategy of two robot arms based on tactile and slippage sensation of optical three-axis tactile sensor system 基于光学三轴触觉传感系统触觉和滑动感觉的机械臂抓取策略

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102221

H. Yussof, S. C. Abdullah, J. Wada, M. Ohka

引用次数: 0

Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids 微旋转流室快速形成胶原凝胶介导的异质球体

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102166

H. Ota, T. Kodama, M. Yamato, T. Okano, N. Miki

{"title":"Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids","authors":"H. Ota, T. Kodama, M. Yamato, T. Okano, N. Miki","doi":"10.1109/MHS.2011.6102166","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102166","url":null,"abstract":"Spheroids that are formed from aggregated cells enhance biological function compared to monolayer culture. In particular, hetero-spheroids composed of different types of cells, such as hepatocytes and endothelial cells, express tissue specific functions at a high level, which is advantageous for more precise drug screening and biological research. In this study, we propose rapid formation of three-dimensional hetero-spheroids consisting of hepatocytes and endothelial cells using micro-rotation flow. The hepatocytes are coated with collagen gel layers less than 200 nm thick to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected in an array by micro-rotational flow and collagen-gel coating, thereby forming hetero-spheroids within 2 min. This array allowed the size of the three-dimensional spheroids to be hydrodynamically controlled by varying the cell density of the medium without altering the device geometry with standard deviations of less than 19%. The proposed microfulidic device, with its capacity of rapidly forming size-controlled hetero-cell aggregates, will offer an efficient experimental platform for heterospheroid study that will contribute to drug screening and regenerative medicine.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121626889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cooperative distributed object classification for multiple robots with audio features 具有音频特征的多机器人协同分布式目标分类

2011 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2011-12-15 DOI: 10.1109/MHS.2011.6102174

Daniel McGibney, T. Umeda, K. Sekiyama, Hiro Mukai, T. Fukuda

引用次数: 4