用于高精度细胞操作和培养的微流体和微加工技术

M. Yamada, M. Seki
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引用次数: 1

摘要

微流控过程不仅是操纵微米级物体(如细胞、颗粒和生物大分子)的基本技术,也是生产具有高度控制形态或成分的微米级物体的基本技术。首先,介绍了利用微流体装置对颗粒或细胞进行连续快速分离和积累的方法。微流控装置有潜力促进快速和精确的颗粒操作,由于精确制造的结构接近颗粒大小,微米或尺寸。新开发的方法使生物细胞或软物质的大小和/或形状依赖,精确分离。接下来,我们提出了用于制备具有纤维或颗粒形态的功能微米级水凝胶材料的微流控装置。所制备材料的物理/化学非均质性使得合并细胞的生长方式不同于传统的平板培养,这对于制备模拟体内组织的单元结构是有用的。除了这些材料外,我们还介绍了最近开发的几种微流控/微加工技术,包括微结构和层状水凝胶板的制备工艺,利用局部表面修饰的超薄水凝胶的微图图化,用于生产驱动脂质囊泡的微喷嘴结构,以及连续微流控细胞的加工。这些技术将有助于通过组装单元材料和/或采用各种常规/非常规微操作技术来快速制造相对较大的组织模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microfluidics and microfabrication technology for highly precise cell manipulation and cultivation
Microfluidic processes are essential techniques not only for manipulating micrometer-size objects like cells, particles, and biomacromolecules, but also for producing micrometer-size objects with highly-controlled morphologies or compositions. First, continuous and rapid separation and accumulation methods for particles or cells using microfluidic devices are introduced. Microfluidic devices have a potential to facilitate rapid and precise particle manipulation, due to accurately fabricated structures close to particle sizes, in micrometer or dimensions. Newly developed methods enable a size- and/or shape-dependent, precise separation of biological cells or soft matters. Next, we present microfluidic devices for preparing functional micrometer-size hydrogel materials having fibrous or particulate morphology. The physical/chemical heterogeneity of the prepared materials allows the incorporated cells to grow differently from the conventional plate cultivation, which is useful for preparing unit structures mimicking the in-vivo tissues. In addition to these materials, here we introduce recently developed several microfluidic/microfabrication techniques, including the preparation processes of microstructured and layered hydrogel plates, micropatterning of ultra-thin hydrogels utilizing local surface modification, micronozzle structures for producing actuating lipid vesicles, and the continuous microfluidic cell processing. These techniques would be useful for rapidly fabricating relatively-large tissue models by assembling the unit materials and/or by employing various conventional/unconventional micromanipulation technologies.
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