{"title":"用于高精度细胞操作和培养的微流体和微加工技术","authors":"M. Yamada, M. Seki","doi":"10.1109/MHS.2011.6102178","DOIUrl":null,"url":null,"abstract":"Microfluidic processes are essential techniques not only for manipulating micrometer-size objects like cells, particles, and biomacromolecules, but also for producing micrometer-size objects with highly-controlled morphologies or compositions. First, continuous and rapid separation and accumulation methods for particles or cells using microfluidic devices are introduced. Microfluidic devices have a potential to facilitate rapid and precise particle manipulation, due to accurately fabricated structures close to particle sizes, in micrometer or dimensions. Newly developed methods enable a size- and/or shape-dependent, precise separation of biological cells or soft matters. Next, we present microfluidic devices for preparing functional micrometer-size hydrogel materials having fibrous or particulate morphology. The physical/chemical heterogeneity of the prepared materials allows the incorporated cells to grow differently from the conventional plate cultivation, which is useful for preparing unit structures mimicking the in-vivo tissues. In addition to these materials, here we introduce recently developed several microfluidic/microfabrication techniques, including the preparation processes of microstructured and layered hydrogel plates, micropatterning of ultra-thin hydrogels utilizing local surface modification, micronozzle structures for producing actuating lipid vesicles, and the continuous microfluidic cell processing. These techniques would be useful for rapidly fabricating relatively-large tissue models by assembling the unit materials and/or by employing various conventional/unconventional micromanipulation technologies.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Microfluidics and microfabrication technology for highly precise cell manipulation and cultivation\",\"authors\":\"M. Yamada, M. Seki\",\"doi\":\"10.1109/MHS.2011.6102178\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Microfluidic processes are essential techniques not only for manipulating micrometer-size objects like cells, particles, and biomacromolecules, but also for producing micrometer-size objects with highly-controlled morphologies or compositions. First, continuous and rapid separation and accumulation methods for particles or cells using microfluidic devices are introduced. Microfluidic devices have a potential to facilitate rapid and precise particle manipulation, due to accurately fabricated structures close to particle sizes, in micrometer or dimensions. Newly developed methods enable a size- and/or shape-dependent, precise separation of biological cells or soft matters. Next, we present microfluidic devices for preparing functional micrometer-size hydrogel materials having fibrous or particulate morphology. The physical/chemical heterogeneity of the prepared materials allows the incorporated cells to grow differently from the conventional plate cultivation, which is useful for preparing unit structures mimicking the in-vivo tissues. In addition to these materials, here we introduce recently developed several microfluidic/microfabrication techniques, including the preparation processes of microstructured and layered hydrogel plates, micropatterning of ultra-thin hydrogels utilizing local surface modification, micronozzle structures for producing actuating lipid vesicles, and the continuous microfluidic cell processing. These techniques would be useful for rapidly fabricating relatively-large tissue models by assembling the unit materials and/or by employing various conventional/unconventional micromanipulation technologies.\",\"PeriodicalId\":286457,\"journal\":{\"name\":\"2011 International Symposium on Micro-NanoMechatronics and Human Science\",\"volume\":\"10 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2011 International Symposium on Micro-NanoMechatronics and Human Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MHS.2011.6102178\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2011 International Symposium on Micro-NanoMechatronics and Human Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2011.6102178","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Microfluidics and microfabrication technology for highly precise cell manipulation and cultivation
Microfluidic processes are essential techniques not only for manipulating micrometer-size objects like cells, particles, and biomacromolecules, but also for producing micrometer-size objects with highly-controlled morphologies or compositions. First, continuous and rapid separation and accumulation methods for particles or cells using microfluidic devices are introduced. Microfluidic devices have a potential to facilitate rapid and precise particle manipulation, due to accurately fabricated structures close to particle sizes, in micrometer or dimensions. Newly developed methods enable a size- and/or shape-dependent, precise separation of biological cells or soft matters. Next, we present microfluidic devices for preparing functional micrometer-size hydrogel materials having fibrous or particulate morphology. The physical/chemical heterogeneity of the prepared materials allows the incorporated cells to grow differently from the conventional plate cultivation, which is useful for preparing unit structures mimicking the in-vivo tissues. In addition to these materials, here we introduce recently developed several microfluidic/microfabrication techniques, including the preparation processes of microstructured and layered hydrogel plates, micropatterning of ultra-thin hydrogels utilizing local surface modification, micronozzle structures for producing actuating lipid vesicles, and the continuous microfluidic cell processing. These techniques would be useful for rapidly fabricating relatively-large tissue models by assembling the unit materials and/or by employing various conventional/unconventional micromanipulation technologies.