European Conference on Biomedical Optics最新文献_第2页

Classification of healthy and pathological human corneas by the analysis of clinical SD-OCT images using machine learning 利用机器学习分析临床SD-OCT图像，对健康和病理人角膜进行分类

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670703

Maëlle Vilbert, Corentin Soubeiran, Benjamin Memmi, C. Georgeon, V. Borderie, A. Chessel, K. Plamann

{"title":"Classification of healthy and pathological human corneas by the analysis of clinical SD-OCT images using machine learning","authors":"Maëlle Vilbert, Corentin Soubeiran, Benjamin Memmi, C. Georgeon, V. Borderie, A. Chessel, K. Plamann","doi":"10.1117/12.2670703","DOIUrl":"https://doi.org/10.1117/12.2670703","url":null,"abstract":"Photorefractive Keratectomy (PRK) is a widely used laser-assisted refractive surgical technique. While generally safe, in some cases it leads to subepithelial inflammation or fibrosis. We here present a robust, machine learning based algorithm for the detection of fibrosis based on Spectral Domain Optical Coherence Tomography (SD-OCT) images recorded in vivo on standard clinical devices. The images first undergo a treatment by a previously developed algorithm for standardisation. The analysis of the pre-treated images allow the extraction of quantitative parameters characterizing the transparency of human corneas. We here propose an extension of this work. Our model is based on 9 morphological quantifiers of the corneal epithelium and in particular of Bowman's layer. In a first step it is trained on SD-OCT images of corneas presenting Fuchs dystrophy, which causes similar symptoms of fibrosis. We trained a Random Forest model for the classification of corneas into \"healthy\" and \"pathological\" classes resulting in a classification accuracy (or success rate) of 97%. The transfer of this same model to images from patients who have undergone Photorefractive Keratectomy (PRK) surgery shows that the model output for probability of healthy classification provides a quantified indicator of corneal healing in the post-operative follow-up. The sensitivity of this probability was studied using repeatability data. We could therefore demonstrate the ability of artificial intelligence to detect sub-epithelial scars identified by clinicians as the origin of post-operative visual haze.","PeriodicalId":278089,"journal":{"name":"European Conference on Biomedical Optics","volume":"64 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126116442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraviolet photoacoustic remote sensing and scattering microscopy for CycleGAN-enabled realistic virtual histology 紫外线光声遥感和散射显微镜为cyclegan启用现实虚拟组织学

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2671022

Matthew T. Martell, Nathaniel J. M. Haven, Ewan A. McAlister, Brendon S. Restall, Brendyn D. Cikaluk, Rohan Mittal, Benjamin A. Adam, Nadia Giannakopoulos, L. Peiris, S. Silverman, Jean-Michaël Deschênes, Xingyu Li, R. Zemp

引用次数: 0

Multifunctional catheter-based optical coherence tomography system for oral cavity and endocervical canal imaging 基于导管的多功能光学相干断层成像系统用于口腔和颈椎管成像

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2669338

Meng-Shan Wu, Chuan-Bor Chueh, Tai-Ang Wang, Ting-Hao Chen, Ting-Yen Tsai, B. Bouma, M. Villiger, Hsiang-Chieh Lee

引用次数: 0

Interrogating Fabry-Perot ultrasound sensors with Bessel beams for photoacoustic imaging 用贝塞尔光束询问法布里-珀罗超声传感器光声成像

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2675686

D. Marques, Oliver J. Sheppard, E. Zhang, P. Beard, P. Munro, J. Guggenheim

引用次数: 0

Wide-field computational cellular-resolution imaging of the human retina using multi-MHz phase-stable SS-OCT 使用多mhz相位稳定SS-OCT的人视网膜宽视场计算细胞分辨率成像

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670648

ByungKun Lee, Sunhong Jeong, Joosung Lee, Tae Shik Kim, B. Braaf, B. Vakoc, W. Oh

{"title":"Wide-field computational cellular-resolution imaging of the human retina using multi-MHz phase-stable SS-OCT","authors":"ByungKun Lee, Sunhong Jeong, Joosung Lee, Tae Shik Kim, B. Braaf, B. Vakoc, W. Oh","doi":"10.1117/12.2670648","DOIUrl":"https://doi.org/10.1117/12.2670648","url":null,"abstract":"Three-dimensional (3D) cellular-resolution imaging of the living human retina over a large field of view would bring a great impact in clinical ophthalmology, potentially finding new biomarkers for early diagnosis and improving the pathophysiological understanding of ocular diseases. While hardware-based and computational Adaptive Optics (AO) Optical Coherence Tomography (OCT) have been developed to achieve cellular-resolution retinal imaging, these approaches support limited 3D imaging fields and their high cost, and their intrinsic hardware complexity limit their practical utility. Here, we demonstrate 3D depth-invariant cellular-resolution imaging of the living human retina over a 3-mm × 3-mm field of view using the intrinsically phase-stable multi-MHz retinal swept-source OCT and tailored computational defocus and aberration correction methods. Single-acquisition imaging of photoreceptor cells, retinal nerve fiber layer, and retinal capillaries is presented across unprecedented imaging fields. By providing wide-field 3D cellular-resolution imaging in the human retina using a standard point-scan architecture routinely used in the clinic, this platform proposes a strategy for expanded utilization of high-resolution retinal imaging in both research and clinical settings.","PeriodicalId":278089,"journal":{"name":"European Conference on Biomedical Optics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131355879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid ultraviolet photoacoustic remote sensing microscopy of fresh tissue sections using voice-coil stage scanning (Withdrawal Notice) 使用声圈级扫描的新鲜组织切片快速紫外光声遥感显微镜(撤回通知)

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670836

Brendyn D. Cikaluk, Brendon S. Restall, Nathaniel J. M. Haven, Matthew T. Martell, Ewan A. McAllister, R. Zemp

引用次数: 0

Three-dimensional reconstruction of subsurface absorbing structures in tissue phantoms from photothermal radiometric records 基于光热辐射记录的组织幻影中亚表面吸收结构的三维重建

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670924

Katja Arh, Tomaž Cvetko, B. Majaron

{"title":"Three-dimensional reconstruction of subsurface absorbing structures in tissue phantoms from photothermal radiometric records","authors":"Katja Arh, Tomaž Cvetko, B. Majaron","doi":"10.1117/12.2670924","DOIUrl":"https://doi.org/10.1117/12.2670924","url":null,"abstract":"Pulsed photothermal radiometry involves measurements of transient changes in blackbody emission from a sample surface after irradiation with a short light pulse. From such a radiometric record, light-induced temperature field inside the sample can be reconstructed by solving the inverse problem of heat diffusion and radiation. In principle, this enables threedimensional visualization of selectively absorbing structures inside strongly scattering biological tissues and organs, a.k.a. photothermal tomography (PTT). We present an up-to-date realization and testing of PTT in an agarose tissue phantom with a suspended human hair, imitating a subsurface blood vessel. After irradiating the phantom with a milisecond laser pulse at 532 nm, its surface was imaged with a fast mid-infrared (IR) camera equiped with a microscope objective. A custom code was used to reconstruct the laser-induced temperature field in three dimensions by running multidimensional optimization based on analytically formulated forward problem of heat transport and IR emission, using the projected -method algorithm. We demonstrate that quadratic binning of the radiometric record enables a 10-fold reduction of the computational time without adversely affecting the results. In the presented example, a sharp image of a hair at a subsurface depth of <200 μm with no significant noise or artifacts elsewhere in the imaged volume of 3 × 3 × 0.6 mm3 was obtained in only 45 seconds.","PeriodicalId":278089,"journal":{"name":"European Conference on Biomedical Optics","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131752248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafast volumetric imaging with a spatio-temporal optical coherence tomography (STOC-T) system for monitoring hemodynamics in mouse retinal vessels directly from structural data 使用时空光学相干断层扫描(stock -t)系统进行超快体积成像，直接从结构数据监测小鼠视网膜血管的血流动力学

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670568

Wiktor M. Kulesza, M. Wielgo, P. Węgrzyn, S. Tomczewski, O. Çetinkaya, Katarzyna Kordecka, Anna Galińska, Bartłomiej Bałamut, E. Auksorius, A. Foik, R. Zawadzki, M. Wojtkowski, D. Borycki, A. Curatolo

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Dual-resolution endoscopic OCT in vivo for improved tissue scattering property characterization 活体双分辨率内镜OCT改善组织散射特性表征

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2670505

T. Cannon, M. Shishkov, Ginger J. Schmidt, M. Villiger, B. Bouma, N. Uribe-Patarroyo

引用次数: 0

Visible-light optical coherence microscopy 可见光光学相干显微镜

European Conference on Biomedical Optics Pub Date : 2023-08-11 DOI: 10.1117/12.2675364

D. Huang, Shanjida Khan, K. Neuhaus, Omkar Thaware, Alireza Karimi, Mary Kelley, Travis Redd, T. Acott, Y. Jian

{"title":"Visible-light optical coherence microscopy","authors":"D. Huang, Shanjida Khan, K. Neuhaus, Omkar Thaware, Alireza Karimi, Mary Kelley, Travis Redd, T. Acott, Y. Jian","doi":"10.1117/12.2675364","DOIUrl":"https://doi.org/10.1117/12.2675364","url":null,"abstract":"Corneal diseases are the fifth leading cause of visual loss globally. Current clinical imaging instruments such as In Vivo Confocal Microscopy (IVCM) offer high lateral resolution to observe cellular structures but lack large field of view and volumetric imaging capability and require high operator skill to focus and align. To overcome these limitations, we have developed blue (450 nm) and green (510 nm) light Optical Coherence Microscopy (OCM) to image cellular structures. Imaging was demonstrated in ex vivo samples including human donor eyes. The OCM systems were based on a spectral-domain optical coherence tomography engine and achieved 750 μm × 750 µm field of view, 1.2 mm imaging depth, and 1.6 μm lateral resolution. Epithelial cells, endothelial cells, and keratocytes of ex vivo rabbit cornea were visualized. Additionally, collagen fibers were observed in stromal lamellae with striated patterns. En face and cross-sectional images of trabecular meshwork and Schlemm’s canal in a donor human eye wedge were observed at various trans-meshwork pressures controlled by cannulation of the canal. Microbes such as filamentous fungi and bacteria were observed.","PeriodicalId":278089,"journal":{"name":"European Conference on Biomedical Optics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123910091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0