Ultrafast volumetric imaging with a spatio-temporal optical coherence tomography (STOC-T) system for monitoring hemodynamics in mouse retinal vessels directly from structural data

Wiktor M. Kulesza, M. Wielgo, P. Węgrzyn, S. Tomczewski, O. Çetinkaya, Katarzyna Kordecka, Anna Galińska, Bartłomiej Bałamut, E. Auksorius, A. Foik, R. Zawadzki, M. Wojtkowski, D. Borycki, A. Curatolo
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Abstract

We present a novel ultrafast imaging system using Spatio-Temporal Optical Coherence Tomography (STOC-T), capable of acquiring structural images of a mouse retina at a volumetric rate of 112 Hz. A calibrated fundus camera and white-light illumination aid the alignment of the mouse and the adjustment of the focal plane in the mouse retina for the STOC-T image. We extract pulsatile blood flow frequency and other hemodynamic parameters from multiple retinal and choroidal vessels from structural-only OCT images, highlighting the prospects of STOC-T for monitoring retinal hemodynamics in a simple way.
使用时空光学相干断层扫描(stock -t)系统进行超快体积成像,直接从结构数据监测小鼠视网膜血管的血流动力学
我们提出了一种使用时空光学相干断层扫描(stock - t)的新型超快成像系统,能够以112 Hz的体积速率获取小鼠视网膜的结构图像。校准后的眼底相机和白光照明有助于小鼠视网膜的对齐和焦平面的调整,以获得stock - t图像。我们从仅结构OCT图像中提取多视网膜和脉络膜血管的脉动血流频率和其他血流动力学参数,突出了sto - t用于简单监测视网膜血流动力学的前景。
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