Chemistry and Physics of Lipids最新文献

{"title":"Novel bioactive lipids enhanced HDL-mediated cholesterol efflux from macrophages through the ABCA1 receptor pathway","authors":"Ali Khattib ,&nbsp;Manar Shmet ,&nbsp;Rasha Ashkar ,&nbsp;Tony Hayek ,&nbsp;Soliman Khatib","doi":"10.1016/j.chemphyslip.2023.105367","DOIUrl":"10.1016/j.chemphyslip.2023.105367","url":null,"abstract":"<div><p>High-density lipoprotein (HDL) has traditionally been acknowledged as \"good cholesterol\" owing to its significant association with a decreased risk of atherosclerosis. This association is primarily attributed to HDL's direct involvement in cholesterol efflux capacity, which plays a pivotal role in reverse cholesterol transport. A novel active compound from <em>Nannochloropsis</em> microalgae termed lyso-DGTS, a lipid that contains EPA fatty acids, was previously isolated and found to increase paraoxonase 1 activity and enhance HDL-mediated cholesterol efflux and HDL-induced endothelial nitric oxide release. Here, the effect of different lyso-DGTS derivatives and analogs on HDL-mediated cholesterol efflux from macrophages was examined, and the mechanism was explored. Structure–activity relationships were established to characterize the essential lipid moieties responsible for HDL-mediated cholesterol efflux from macrophages. Lyso-DGTS, 1-carboxy-N-N-N-trimethyl-3-oleamidopropan-1-aminium, and lyso-platelet-activating factor increased HDL-mediated cholesterol efflux from macrophages dose-dependently, mainly via the ABCA1-mediated cholesterol efflux pathway. The effect of lyso-DGTS derivatives and analogs on the surface polarity of HDL was examined using the Laurdan generalized polarization (GP) assay. A reverse Pearson linear regression was obtained between Laurdan GP values and HDL-mediated cholesterol efflux. Because the incorporation of bioactive lipids into the surface phospholipid layer of HDL leads to a decrease in Laurdan GP, these bioactive lipids may induce lower phospholipid ordering and greater free space on the HDL particle surface, thereby enhancing apolipoprotein A1 binding to the ABCA1 receptor and improving ABCA1 cholesterol-mediated efflux. Our findings suggest a beneficial effect of lyso-DGTS and its bioactive lipid derivatives on increasing HDL-mediated cholesterol efflux activity from macrophages, which may impact atherosclerosis attenuation.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105367"},"PeriodicalIF":3.4,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000890/pdfft?md5=7ea020d7c9ebc26c842f5b41912c42ee&pid=1-s2.0-S0009308423000890-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural changes in layers of lipid mixtures at low surface tensions","authors":"A.G. Bykov ,&nbsp;M.A. Panaeva ,&nbsp;O.Y. Milyaeva ,&nbsp;A.V. Michailov ,&nbsp;A.R. Rafikova ,&nbsp;E. Guzman ,&nbsp;R. Rubio ,&nbsp;R. Miller ,&nbsp;B.A. Noskov","doi":"10.1016/j.chemphyslip.2023.105365","DOIUrl":"10.1016/j.chemphyslip.2023.105365","url":null,"abstract":"<div><p>Layers of pulmonary lipids on an aqueous substrate at non-equilibrium conditions can decrease the surface tension of water to quite low values. This is connected with different relaxation processes occurring at the interface and the associated changes in the surface layer structure. Results of measurements by the combination of methods like surface rheology, ellipsometry, Brewster angle microscopy, and IRRAS for spread layers of lipid mixtures open a possibility to specify the dynamics of structural changes at conditions close to the physiological state. At sufficiently low surface tension values (below 5 mN/m) significant changes in the ellipsometric signal were observed for pure DPPC layers, which can be related to a transition from 2D to 3D structures caused by the layer folding. The addition of other lipids can accelerate the relaxation processes connected with squeezing-out of molecules or multilayer stacks formation hampering thereby a decrease of surface tension down to low values corresponding to the folding of the monolayer.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105365"},"PeriodicalIF":3.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000877/pdfft?md5=c6ea824731de0addde49f7c4c3e09c51&pid=1-s2.0-S0009308423000877-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closer look at the calorimetric lower transition in lipid bilayers","authors":"Sophia A. Korono,&nbsp;John F. Nagle","doi":"10.1016/j.chemphyslip.2023.105366","DOIUrl":"10.1016/j.chemphyslip.2023.105366","url":null,"abstract":"<div><p>The thermal behavior of unilamellar vesicles has been revisited with differential scanning calorimetry to address the issue of whether it is essential to include interactions between neighboring bilayers in theories and simulations of the ripple phase. The issue focuses on the lower, <em>aka</em> pretransition, and the ripple phase that clearly exists between the lower and main transitions in multilamellar vesicles (MLV). We find anomalous thermal behavior in unilamellar vesicles (ULV) beginning at the same temperature as the lower transition in MLVs, but this feature is considerably broadened and somewhat weaker compared to the lower transition in MLVs. We ascribe this to the difficulty of packing a regular ripple pattern on small spheres. In agreement with a few reports of a ripple phase in direct images of single bilayers, we conclude that interactions between neighboring bilayers are not essential for the ripple phase in lipid bilayers.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105366"},"PeriodicalIF":3.4,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000889/pdfft?md5=40ff883edcd280818ebff926ab6c6002&pid=1-s2.0-S0009308423000889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cationic dendrimer on membrane mimetic systems in the form of monolayer and bilayer","authors":"Biplab Roy ,&nbsp;Pritam Guha ,&nbsp;Chien-Hsiang Chang ,&nbsp;Prasant Nahak ,&nbsp;Gourab Karmakar ,&nbsp;Alexey G. Bykov ,&nbsp;Alexander V. Akentiev ,&nbsp;Boris A. Noskov ,&nbsp;Anuttam Patra ,&nbsp;Kunal Dutta ,&nbsp;Chandradipa Ghosh ,&nbsp;Amiya Kumar Panda","doi":"10.1016/j.chemphyslip.2023.105364","DOIUrl":"10.1016/j.chemphyslip.2023.105364","url":null,"abstract":"<div><p>Interactions between a zwitterionic phospholipid, 1, 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and four anionic phospholipids dihexadecyl phosphate (DHP), 1, 2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1, 2-dipalmitoyl-sn-glycero-3-phosphate (DPP) and 1, 2-dipalmitoyl-sn-glycero-3-phospho ethanol (DPPEth) in combination with an additional amount of 30 mol% cholesterol were separately investigated at air-buffer interface through surface pressure (π) - area (A) measurements. π-A isotherm derived parameters revealed maximum negative deviation from ideality for the mixtures comprising 30 mol% anionic lipids. Besides the film functionality, structural changes of the monomolecular films at different surface pressures in the absence and presence of polyamidoamine (PAMAM, generation 4), a cationic dendrimer, were visualised through Brewster angle microscopy and fluorescence microscopic studies. Fluidity/rigidity of monolayers were assessed by surface dilatational rheology studies. Effect of PAMAM on the formation of adsorbed monolayer, due to bilayer disintegration of liposomes (DPPC:anionic lipids= 7:3 M/M, and 30 mol% cholesterol) were monitored by surface pressure (π) - time (t) isotherms. Bilayer disintegration kinetics were dependent on lipid head group and chain length, besides dendrimer concentration. Such studies are considered to be an in vitro cell membrane model where the alteration of molecular orientation play important roles in understanding the nature of interaction between the dendrimer and cell membrane. Liposome-dendrimer aggregates were nontoxic to breast cancer cell line as well as in doxorubicin treated MDA-MB-468 cell line suggesting their potential as drug delivery systems.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105364"},"PeriodicalIF":3.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000865/pdfft?md5=c4c51323e058927cf3eee1a594e7134f&pid=1-s2.0-S0009308423000865-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138469583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unsaturation of serine lipids modulating the interaction of a cytosporone with models of the external leaflet of tumorigenic cell membranes","authors":"Guilherme Nuñez Jaroque,&nbsp;Augusto Leonardo dos Santos,&nbsp;Patrícia Sartorelli,&nbsp;Luciano Caseli","doi":"10.1016/j.chemphyslip.2023.105363","DOIUrl":"10.1016/j.chemphyslip.2023.105363","url":null,"abstract":"<div><p>Cytosporone-B was isolated from fungi and incorporated in models of tumorigenic cell membranes using palmitoyloleoylglycerophosphoserine (POPS) and dipalmitoyl glycerophosphoserine (DPPS) lipids. While for DPPS, the compound condensed the monolayer and decreased the surface compressional modulus, it expanded and kept the compressional modulus for POPS. Hysteresis for compression-expansion cycles was more sensitive for POPS than for DPPS, while a high degree of destabilization was observed for POPS. As observed with infrared spectroscopy and Brewster angle microscopy, specific changes were selective regarding molecular organization and morphology. Atomic force microscopy for transferred monolayers as Langmuir-Blodgett films also confirmed such specificities. We believe these data can help understand the mechanism of action of bioactive drugs in lipid interfaces at the molecular level.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105363"},"PeriodicalIF":3.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000853/pdfft?md5=f997a61328a6651b49243b8ca79b906d&pid=1-s2.0-S0009308423000853-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138476416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between macrophage membrane and lipid mediators during cardiovascular diseases with the implications of scavenger receptors","authors":"Sangeetha Ravi ,&nbsp;Livya Catherene Martin ,&nbsp;Mahalakshmi Krishnan ,&nbsp;Manikandan Kumaresan ,&nbsp;Beulaja Manikandan ,&nbsp;Manikandan Ramar","doi":"10.1016/j.chemphyslip.2023.105362","DOIUrl":"10.1016/j.chemphyslip.2023.105362","url":null,"abstract":"<div><p>The onset and progression of cardiovascular diseases with the major underlying cause being atherosclerosis, occur during chronic inflammatory persistence in the vascular system, especially within the arterial wall. Such prolonged maladaptive inflammation is driven by macrophages and their key mediators are generally attributed to a disparity in lipid metabolism. Macrophages are the primary cells of innate immunity, endowed with expansive membrane domains involved in immune responses with their signalling systems. During atherosclerosis, the membrane domains and receptors control various active organisations of macrophages. Their scavenger/endocytic receptors regulate the trafficking of intracellular and extracellular cargo. Corresponding influence on lipid metabolism is mediated by their dynamic interaction with scavenger membrane receptors and their integrated mechanisms such as pinocytosis, phagocytosis, cholesterol export/import, etc. This interaction not only results in the functional differentiation of macrophages but also modifies their structural configurations. Here, we reviewed the association of macrophage membrane biomechanics and their scavenger receptor families with lipid metabolites during the event of atherogenesis. In addition, the membrane structure of macrophages and the signalling pathways involved in endocytosis integrated with lipid metabolism are detailed. This article establishes future insights into the scavenger receptors as potential targets for cardiovascular disease prevention and treatment.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105362"},"PeriodicalIF":3.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000841/pdfft?md5=17934960823409dc7f16ffeb9760fdd9&pid=1-s2.0-S0009308423000841-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138439974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring galactolipid digestion and simultaneous changes in lipid-bile salt micellar organization by real-time NMR spectroscopy","authors":"Moulay Sahaka ,&nbsp;Olivier Bornet ,&nbsp;Achille Marchand ,&nbsp;Dominique Lafont ,&nbsp;Brigitte Gontero ,&nbsp;Frédéric Carrière ,&nbsp;Hélène Launay","doi":"10.1016/j.chemphyslip.2023.105361","DOIUrl":"10.1016/j.chemphyslip.2023.105361","url":null,"abstract":"<div><p>The use of Nuclear Magnetic Resonance spectroscopy for studying lipid digestion in vitro most often consists of quantifying lipolysis products after they have been extracted from the reaction medium using organic solvents. However, the current sensitivity level of NMR spectrometers makes possible to avoid the extraction step and continuously quantify the lipids directly in the reaction medium. We used real-time ¹H NMR spectroscopy and guinea pig pancreatic lipase-related protein 2 (GPLRP2) as biocatalyst to monitor in situ the lipolysis of monogalactosyl diacylglycerol (MGDG) in the form of mixed micelles with the bile salt sodium taurodeoxycholate (NaTDC). Residual substrate and lipolysis products (monogalactosyl monoacylglycerol (MGMG); monogalactosylglycerol (MGG) and octanoic acid (OA) were simultaneously quantified throughout the reaction thanks to specific proton resonances. Lipolysis was complete with the release of all MGDG fatty acids. These results were confirmed by thin layer chromatography (TLC) and densitometry after lipid extraction at different reaction times. Using diffusion-ordered NMR spectroscopy (DOSY), we could also estimate the diffusion coefficients of all the reaction compounds and deduce the hydrodynamic radius of the lipid aggregates in which they were present. It was shown that MGDG-NaTDC mixed micelles with an initial hydrodynamic radius r_H of 7.3 ± 0.5 nm were changed into smaller micelles of NaTDC-MGDG-MGMG of 2.3 ± 0.5 nm in the course of the lipolysis reaction, and finally into NaTDC-OA mixed micelles (r_H of 2.9 ± 0.5 nm) and water soluble MGG. These results provide a better understanding of the digestion of galactolipids by PLRP2, a process that leads to the complete micellar solubilisation of their fatty acids and renders their intestinal absorption possible.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105361"},"PeriodicalIF":3.4,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S000930842300083X/pdfft?md5=7949f0a6afc8b93348193638444baadc&pid=1-s2.0-S000930842300083X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138045681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the functional role of human ABHD16B lipase in regulating triacylglycerol mobilization and membrane lipid synthesis in Saccharomyces cerevisiae","authors":"Raja Narayanasamy ,&nbsp;Dandamudi Usharani ,&nbsp;Ram Rajasekharan","doi":"10.1016/j.chemphyslip.2023.105353","DOIUrl":"10.1016/j.chemphyslip.2023.105353","url":null,"abstract":"<div><p>Lipids are essential biological macromolecules that play a pivotal role in various physiological processes and cellular homeostasis. ABHD16B, a member of the α/β-hydrolase domain (ABHD) superfamily protein, has emerged as a potential key regulator in lipid metabolism. However, the precise role of human ABHD16B in lipid metabolism remains unclear. In this study, we reported the overexpression of ABHD16B in <em>Saccharomyces cerevisiae</em> to determine its physiological relevance in lipid metabolism. Through <em>in vivo</em> [¹⁴C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrometry (LC–MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal microscopy analysis revealed a reduction in the number of lipid droplets in strains overexpressing ABHD16B, consistent with the observed decrease in neutral lipids. Additionally, qRT-PCR analysis indicated a high phospholipid synthetic activity of ABHD16B and a potential decrease in TAG levels in wild-type yeast, possibly due to upregulation of endogenous TAG hydrolytic enzymes, as confirmed using <em>3tgls</em>Δ mutant strain. Furthermore, GC-MS analysis revealed significant modifications in fatty acid composition upon ABHD16B overexpression. Collectively, our results underscore the influence of ABHD16B overexpression on TAG levels, phospholipid synthesis, lipid droplet dynamics, and fatty acid composition. These findings reveal a complex interplay between TAG hydrolysis and phospholipid synthesis, highlighting the critical involvement of ABHD16B in lipid homeostasis and providing further insights into its regulatory function in cellular lipid metabolism.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105353"},"PeriodicalIF":3.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000750/pdfft?md5=88c09caa313909654509f6e549ded1c5&pid=1-s2.0-S0009308423000750-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72012831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tribute to our friend and colleague Professor Richard M. Epand","authors":"Jose C. Bozelli Jr.,&nbsp;Raquel F. Epand,&nbsp;John Katsaras,&nbsp;Jesús Pérez-Gil","doi":"10.1016/j.chemphyslip.2023.105352","DOIUrl":"https://doi.org/10.1016/j.chemphyslip.2023.105352","url":null,"abstract":"","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"257 ","pages":"Article 105352"},"PeriodicalIF":3.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91962724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidomic analysis identified potential predictive biomarkers of statin response in subjects with Familial hypercholesterolemia","authors":"Alvaro Cerda ,&nbsp;Raul Hernandes Bortolin ,&nbsp;Marcos Yukio Yoshinaga ,&nbsp;Renata Caroline Costa de Freitas ,&nbsp;Carolina Dagli-Hernandez ,&nbsp;Jessica Bassani Borges ,&nbsp;Victor Fernandes de Oliveira ,&nbsp;Rodrigo Marques Gonçalves ,&nbsp;Andre Arpad Faludi ,&nbsp;Gisele Medeiros Bastos ,&nbsp;Rosario Dominguez Crespo Hirata ,&nbsp;Mario Hiroyuki Hirata","doi":"10.1016/j.chemphyslip.2023.105348","DOIUrl":"10.1016/j.chemphyslip.2023.105348","url":null,"abstract":"<div><p><span><span>Familial hypercholesterolemia (FH) is a disorder of lipid metabolism that causes elevated low-density lipoprotein cholesterol (LDL-c) and increased premature atherosclerosis risk. </span>Statins<span><span><span> inhibit endogenous cholesterol biosynthesis, which reduces LDL-c plasma levels and prevent from cardiovascular events. This study aimed to explore the effects of statin treatment on serum </span>lipidomic<span> profile and to identify biomarkers of response in subjects with FH. Seventeen adult FH patients underwent a 6-week washout followed by 4-week treatment with atorvastatin (80 mg/day) or </span></span>rosuvastatin<span><span><span> (40 mg/day). LDL-c response was considered good (40–70 % reduction, n = 9) or poor (3–33 % reduction, n = 8). Serum lipidomic profile was analyzed by ultra-high-performance liquid chromatography combined with electrospray ionization tandem time-of-flight mass spectrometry, and data were analyzed using MetaboAnalyst v5.0. Lipidomic analysis identified 353 </span>lipids grouped into 16 classes. Statin treatment reduced drastically 8 of 13 lipid classes, generating a characteristic lipidomic profile with a significant contribution of </span>phosphatidylinositols (PI) 16:0/18:2, 18:0/18:1 and 18:0/18:2; and </span></span></span>triacylglycerols<span> (TAG) 18:2x2/18:3, 18:1/18:2/18:3, 16:1/18:2x2, 16:1/18:2/18:3 and 16:1/18:2/Arachidonic acid (p-adjusted &lt;0.05). Biomarker analysis implemented in MetaboAnalyst subsequently identified PI 16:1/18:0, 16:0/18:2 and 18:0/18:2 as predictors of statin response with and receiver operating characteristic (ROC) areas under the curve<span> of 0.98, 0.94 and 0.91, respectively. In conclusion, statins extensively modulate the overall serum lipid<span> composition of FH individuals and these findings suggest that phosphatidyl-inositol molecules are potential predictive biomarkers of statin response.</span></span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"257 ","pages":"Article 105348"},"PeriodicalIF":3.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41186896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}