细菌总脂提取物的纳米盘组装。

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2024-08-05 DOI:10.1016/j.chemphyslip.2024.105425

Trent R. Llewellyn , Olivia R.C. Pimentel , Kiersten D. Lenz, Makaela M. Montoya, Jessica Z. Kubicek-Sutherland

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引用次数: 0

摘要

纳米盘是一种盘状脂蛋白，经常被用作研究原生构型膜蛋白的载体。纳米光盘主要由合成脂质制成。然而，纳米光盘也提供了一种可以研究天然构型的原生脂质的形式。在这里，我们介绍了一种从细菌总脂质提取物中合成纳米微粒的方法，并使用生物威胁制剂鼠疫耶尔森菌作为概念验证。这种完全由细菌脂质组成的纳米颗粒有助于膜表征和疫苗抗原的发现，而不会产生与这种一级选择性病原体的活细菌细胞相关的固有安全问题。

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Nanodisc assembly from bacterial total lipid extracts

Nanodiscs are discoidal lipoproteins that have often been used as vehicles to study membrane proteins in their native configuration. Nanodiscs have been primarily made from synthetic lipids. However, nanodiscs also offer a format by which native lipids can be studied in their natural configuration. Here, we present a method to synthesize nanodiscs from bacterial total lipid extracts using the biothreat agent, Yersinia pestis, as a proof-of-concept. The creation of nanoparticles entirely composed of bacterial lipids supports membrane characterization and vaccine antigen discovery without the inherent safety concerns associated with live bacterial cells of this Tier 1 select agent pathogen.

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.