Rapid screening of prednisolone acetate adulterants in health foods using colloidal gold immunochromatographic assay

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2024-10-04 DOI:10.1016/j.chemphyslip.2024.105447

Sayed Haidar Abbas Raza , Zixin Huang , Yimeng Pang , Ruimin Zhong , Xiangmei Li , Sameer D. Pant , Lin Luo , Hongtao Lei

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Abstract

In this study, a rapid detection method utilizing colloidal gold immunochromatography (CG-ICA) was developed for the detection of illegally added prednisone acetate in health foods. Initially, the preparation conditions of colloidal gold solution were optimized. The optimal potassium carbonate dosage, antibody diluent type, antibody dosage, probe labeling time, blocking time and BSA dosage were determined. Technical analysis was performed to ensure that the established CG-ICA exhibited satisfactory color development and inhibition rates. Under optimized conditions, the cut-off value of CG-ICA was 250 μg/kg. The assay demonstrated a sensitivity of 100 %, a false positive rate of 8 %, and a false negative rate of 0, indicating high specificity for prednisone acetate. The results obtained from testing actual samples were consistent with those obtained using LC-MS/MS, thereby verifying the reliability of the developed method. This method offers robust support for the rapid detection of illegally added prednisone acetate in health foods.

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利用胶体金免疫层析测定法快速筛查保健食品中的醋酸泼尼松龙掺假物

本研究利用胶体金免疫层析技术（CG-ICA）建立了一种快速检测方法，用于检测保健食品中非法添加的醋酸泼尼松。首先，对胶体金溶液的制备条件进行了优化。确定了最佳的碳酸钾用量、抗体稀释剂类型、抗体用量、探针标记时间、阻断时间和 BSA 用量。进行了技术分析，以确保所建立的 CG-ICA 具有令人满意的显色性和抑制率。在优化条件下，CG-ICA 的临界值为 250μg/kg。检测灵敏度为 100%，假阳性率为 8%，假阴性率为 0，表明醋酸泼尼松的特异性很高。实际样品检测结果与 LC-MS/MS 检测结果一致，从而验证了所开发方法的可靠性。该方法为快速检测保健食品中非法添加的醋酸泼尼松提供了有力的支持。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.