Journal of Jahrom University of Medical Sciences最新文献_第3页

Effect of propofol anesthesia on skin flap survival in rat; comparison with ketamine 异丙酚麻醉对大鼠皮瓣存活的影响氯胺酮的比较

Journal of Jahrom University of Medical Sciences Pub Date : 2016-12-15 DOI: 10.29252/JMJ.14.4.62

A. R. Jahromi, A. T. Naeini, S. Nazifi, Z. Seif

{"title":"Effect of propofol anesthesia on skin flap survival in rat; comparison with ketamine","authors":"A. R. Jahromi, A. T. Naeini, S. Nazifi, Z. Seif","doi":"10.29252/JMJ.14.4.62","DOIUrl":"https://doi.org/10.29252/JMJ.14.4.62","url":null,"abstract":"Introduction: This study aimed to evaluate non-anesthetic properties of propofol in skin flap survival in rats. Materials and methods: Thirty-two adult white female rats were randomly divided into two groups. Group 1 was anesthesized with ketamine (40 mg/kg) and group 2 was anesthesized with propofol (100 mg/kg) intraperitoneally. A full-thickness piece of skin on the back of rats (2.5 × 8 × 8 cm) was incised while the flap base was preserved. The flap was immediately returned and sutured using 5.0 separate sutures to the original location. Apparent healing was daily assessed by measuring the necrotic and healthy areas and the survival rate of the skin flaps was evaluated after photography. Histopathological evaluation was performed by a blinded pathologist on days 1, 4, 7 and 21 in three zones of the flap: up, down and middle. Serum interleukin 1 and 6 were measured before and 12, 24 and 168 hours after creating the flaps. Results: Flap survival rate on day 21, and IL-6 on day 0, 12 hour and day 1 were significantly higher in propofol group than in ketamine group. IL-1 was not significantly different. Histological signs of healing were more prominent in propofol group. Conclusion: It appears that propofol has a more significant effect on skin flap survival in comparison with ketamine, but further and more precise studies are required to confirm this finding.","PeriodicalId":263834,"journal":{"name":"Journal of Jahrom University of Medical Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131361075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning of tagD gene from Helicobacter pylori in PFLAG-CMV-3 eukaryotic vector to generate a DNA vaccine PFLAG-CMV-3真核载体中幽门螺杆菌tagD基因克隆制备DNA疫苗

Journal of Jahrom University of Medical Sciences Pub Date : 2016-12-15 DOI: 10.29252/JMJ.14.4.43

مریم صفرپور, زهرا کاظمی, الهام دوستی, عباس دوستی

{"title":"Cloning of tagD gene from Helicobacter pylori in PFLAG-CMV-3 eukaryotic vector to generate a DNA vaccine","authors":"مریم صفرپور, زهرا کاظمی, الهام دوستی, عباس دوستی","doi":"10.29252/JMJ.14.4.43","DOIUrl":"https://doi.org/10.29252/JMJ.14.4.43","url":null,"abstract":"Introduction: Helicobacter pylori is strongly associated with gastritis, stomach cancer, gastric lymphoma and peptic ulcer in human. Thiol peroxidase is encoded by tagD gene and plays a significant role in colonizing H. pylori in the stomach. The product of tagD gene stimulates the immune system in the host. This study aimed to isolate and clone tagD gene in the eukaryotic expression vector PFLAG-CMV-3 as a DNA vaccine candidate. Materials and methods: In this experimental research, tagD gene (537 bp) was amplified by PCR. The PCR products were cloned using cloning commercial kits (Thermo Fisher Co., COUNTRY) in pTZ vector. This gene was subcloned in the eukaryotic expression vector (PFLAG-CMV-3 vector), then transferred into CHO cells by electroporation method and was expressed. Results: The results indicate that amplification and cloning of tagD gene was successful, and the pTZ-tagD vector was formed. PFLAG-CMV-3 vector construction was confirmed by digestion and gene sequencing. The 19 kDa band was observed by gene expression analysis on SDS-PAGE. Conclusions: tagD gene in the PFLAG-CMV-3-tagD recombinant vector has the ability to produce specific protein in CHO cells. Therefore, this gene construct is useful to evaluate the immunogenicity as a DNA vaccine against H. pylori infection in animal models.","PeriodicalId":263834,"journal":{"name":"Journal of Jahrom University of Medical Sciences","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122546128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The epidemiology of Tuberculosis in Jahrom (2005-2014) 贾罗姆市2005-2014年结核病流行病学分析

Journal of Jahrom University of Medical Sciences Pub Date : 2016-12-15 DOI: 10.29252/JMJ.14.4.16

وحید رحمانیان, کرامت اله رحمانیان, علیرضا صفری, الهام منصوریان, محمد علی راستگوفر

引用次数: 6

Evaluation of Carbapenemase resistance in Pseudomonas aeruginosa and Enterobacteriaceae family isolated from clinical specimens by using phenotypic methods in 2014-2015 2014-2015年铜绿假单胞菌和肠杆菌科临床分离株碳青霉烯酶耐药性的表型分析

Journal of Jahrom University of Medical Sciences Pub Date : 2016-12-10 DOI: 10.29252/JMJ.14.4.8

tahmineh ebrahimzadeh shiraz, H. Yazdi, M. Alijanianzadeh

{"title":"Evaluation of Carbapenemase resistance in Pseudomonas aeruginosa and Enterobacteriaceae family isolated from clinical specimens by using phenotypic methods in 2014-2015","authors":"tahmineh ebrahimzadeh shiraz, H. Yazdi, M. Alijanianzadeh","doi":"10.29252/JMJ.14.4.8","DOIUrl":"https://doi.org/10.29252/JMJ.14.4.8","url":null,"abstract":"Introduction: Carbapenems is on second line therapy of multidrug resistant Pseudomonas aeruginosa infections and are the last defense line in critical infections Enterobacteriaceae family but Carbapenems resistance is increased so there are some problems when Carbapenems resistance is increased. Therefore, a new study about carbapenem resistance is necessary. Materials and Methods: This study collected 196 isolated bacteria of Pseudomonas aeruginosa and Enterobacteriaceae family from Hospitals patient of Tehran and evaluates them by phenotypic and biochemical methods. Also, the researcher studies the disk diffusion method and use it to determine antibiotic sensitivity of all bacteria according to the CLSI standard table. Results: From the total of 196 bacteria collected, the resistance to Meropenem is %39.79 and Imipenem is %45.91 and 30.10 percent to Meropenem and Imipenem resistance so that the percent resistance of bacteria is as follows: Pseudomonas aeruginosa resistance to Meropenem is %39.62 and Imipenem is %43.39. Meropenem and Imipenem resistance in Enterobacteriaceae family are as follows: Salmonella resistance to Imipenem and Meropenem is %0 and %50, Citrobacter koseri the resistance to Imipenem and Meropenem %0 and %50, Citrobacter freundii Meropenem resistance of %28.57 and Imipenem %28.57, Escherichia coli resistance to Meropenem %39.70 and Imipenem %47.05, Klebsiella oxytoca resistance to Meropenem %46.6 and %40 Imipenem and Meropenem resistance Klebsiella pneumoniae %34.14 and Imipenem %53.65, respectively. Conclusion: There is an increasing resistance to Meropenem and Imipenem antibiotics in treatment of Pseudomonas aeruginosa and Enterobacteriaceae family in our country. Hence, it should be properly and reasonably use these antibiotics.","PeriodicalId":263834,"journal":{"name":"Journal of Jahrom University of Medical Sciences","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115765868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Comparison of direct PCR and PCR using DNA extracted kit for determine tst and mecA genes in Staphylococcus aureus 直接PCR与DNA提取试剂盒PCR检测金黄色葡萄球菌tst和mecA基因的比较

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/jmj.14.3.43

H. Tahmasebi, M. Bokaeian, Mojdeh Jahantigh, J. Adabi

引用次数: 0

Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method 小鼠新生皮肤成纤维细胞的分离与培养

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/JMJ.14.3.35

Alireza Kheirollah, Mostafa Chashmpoosh, A. Mohammadi, Neda Abdovis, Hamide Ghasempoor

引用次数: 1

The frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococci 耐甲氧西林凝固酶阴性葡萄球菌临床分离株qacA/B和smr基因的频率

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/JMJ.14.3.8

محمد بکائیان, جواد ادبی, حامد طهماسبی

{"title":"The frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococci","authors":"محمد بکائیان, جواد ادبی, حامد طهماسبی","doi":"10.29252/JMJ.14.3.8","DOIUrl":"https://doi.org/10.29252/JMJ.14.3.8","url":null,"abstract":"Introduction: Increasing use of disinfectants biocide cause to appearance of resistant strains of coagulase negative Staphylococcus. Some research confirmed this gene responsible for resistance to methicillin and association with these agents. The aim of this study was to investigate the presence of resistance genes of biocides such as qac A/B and smr in coagulase negative staphylococci. Materials & Methods: In this cross-sectional study, 60 samples of Staphylococcus epidermidis and 49 samples of Staphylococcus saprophyticus were collected over 9 months from clinical samples. After the initial biochemical tests and confirmation of genus and species of isolates, specific primers were used to study aqcA/B and smr genes through polymerase chain reaction (PCR). Data were analyzed using chisquare test. Results: Of 60 isolates of Staphylococcus saprophyticus, 36 isolates (60%) had mecA. Among these, 19 isolates (52.77%) had qacA and 21 isolates (58.33%) had smr. Furthermore, of 49 Staphylococcus epidermidis isolates, 27 isolates (55.1%) had mecA and among those isolates, 11 isolates (22.44%) had qac A/B and 8 isolate (16.32%) had smr. Conclusions: The results of our study showed the widespread presence of qac A/B and smr in clinical isolates of methicillin-resistant coagulase-negative staphylococci. Given the low frequency of qacA/B and smr in the isolates sensitive to the antibiotics, it is necessary to evaluate antibiotic resistance for treatment of microbial infections.","PeriodicalId":263834,"journal":{"name":"Journal of Jahrom University of Medical Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125723389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Enterococci faecalis&E. faeaciumpathogens viaTehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016 粪肠球菌的鉴定。2016年德黑兰医院病原菌临床标本表型和基因分型及药敏评价

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/JMJ.14.3.52

Sara Masoumi Zavaryani, Reza Mirnejd, Shohreh Zareh, Vahhab Piranfar, Ozra Bagheri Bejestani

{"title":"Identification of Enterococci faecalis&E. faeaciumpathogens viaTehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016","authors":"Sara Masoumi Zavaryani, Reza Mirnejd, Shohreh Zareh, Vahhab Piranfar, Ozra Bagheri Bejestani","doi":"10.29252/JMJ.14.3.52","DOIUrl":"https://doi.org/10.29252/JMJ.14.3.52","url":null,"abstract":"Introduction: Epidemiology of Enterococcus infections has attracted much attention. Precise identification of pathogenic strains can be effective in the control process of microorganism antibiotic resistance. This study aimed to identify the Enterococcus species isolated from hospitals in Tehran and examine their antibiotic resistance pattern by phenotypic and genotypic methods. Methods & Materials: This study was performed on 400 clinical samples from different hospitals in Tehran during 20152016. Specific cultures and biochemical tests were used to identify Enterococcus, distinguish E. faecalis and E. faeacium species and PCR method was used to identify Enterococcus species. Antibiotic susceptibility was examined using Kirby-Bauer disc diffusion, and CLSI and vancomycin MIC were measured using broth dilution. Results: Of the 400 samples, 278 Enterococcus species were recognized. Phenotypic methods recognized 70.86% E. faecalis, 15.46% E. faeacium and 13.68% other species. PCR identified 72.3% E. faecalis, 10.43% E. faeacium and 17.27% other Enterococcus species. Results of the antibiograms showed the highest resistance (83.34%) to quinupristin/dalfopristin, and the lowest (1.41%) to linezolid. Also, resistance to vancomycin was observed in 5.95% with MIC ≥ 512 μg/ml in 9 cases. Conclusion: Rapid diagnosis can prevent massive outbreaks of Enterococcus. Given the prevalence of vancomycin resistance in Enterococcus, preventive measures are imperative. The right antibiotics should be prescribed according to the resistance patterns after susceptibility test is performed for each patient.","PeriodicalId":263834,"journal":{"name":"Journal of Jahrom University of Medical Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128111912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of cinnamon on the Elimination of lead poisoning on sex hormone changes in adult female rats 肉桂消除铅中毒对成年雌性大鼠性激素变化的影响

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/jmj.14.3.1

Parvin Bagherzadeh

引用次数: 1

The Effect of resistance training circuit with Medicago sativa extracts on levels of Osteoprotegerin and Nuclear Factor of Kappa-B in thin girls 紫花苜蓿提取物抗阻训练回路对瘦女孩骨保护素和κ b核因子水平的影响

Journal of Jahrom University of Medical Sciences Pub Date : 2016-07-15 DOI: 10.29252/JMJ.14.3.27

پروین فرزانگی, خاطره ابراهیمی, معصومه حبیبیان

引用次数: 2