Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method

Abstract

Introduction: Fibroblasts are an important component of connective tissue found almost everywhere in the body. Fibroblasts have different functions and morphologies based on their location. This project aimed to simplify isolating newborn mouse skin fibroblasts for use in future studies. Methods & Materials: First, Balb/c neonate mice of one to two days old were slaughtered under laminar hood using sterilized tools. Then, skin fibroblasts were isolated using trypsin 1% and cultured. Finally, the cells were transferred to a new growth medium for the first passage. Results: Fibroblasts were evaluated in different days in terms of growth and morphology by a fluorescent microscope. Cells with clear oval or spindle-shaped nucleus with one or two nuclei were observed, which correspond with fibroblast morphology. The culture media were clear and showed no opacity or microbial or fungal contamination. Conclusion: Based on the results, it is logical to use this method because it is simple, inexpensive and sterile, so it does not require antibiotics and antifungal treatment.