Carbohydrate Polymers最新文献_第5页

Production of flame-retardant phosphorylated cellulose nanofibrils by choline chloride based reactive deep eutectic solvent 利用基于氯化胆碱的反应性深共晶溶剂生产阻燃磷化纤维素纳米纤丝

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122931

Yutong Zhang , Lebin Zhao , Yun Liu , Chaohong Dong , Kaitao Zhang

{"title":"Production of flame-retardant phosphorylated cellulose nanofibrils by choline chloride based reactive deep eutectic solvent","authors":"Yutong Zhang , Lebin Zhao , Yun Liu , Chaohong Dong , Kaitao Zhang","doi":"10.1016/j.carbpol.2024.122931","DOIUrl":"10.1016/j.carbpol.2024.122931","url":null,"abstract":"<div><div>Nanocellulose, a biomass resource known for its abundance, renewability, environmental friendliness, and nanoscale size, has garnered significant interest from researchers. However, it is a type of carbohydrate that burns very easily, which limits its applications, especially in areas where good thermal stability and low flammability are requested. In this study, phosphorylated cellulose fibers (P-CF) was prepared via ternary choline chloride/urea/ phosphorous acid reactive deep eutectic solvent (RDES) pretreatment. The influences of different conditions (reaction temperature, time, mass ratio of cellulose to RDES and molar ratio of choline chloride/urea/ phosphorous acid) on the charge density and yield of P-CF were studied. Phosphorylated cellulose nanofibrils (P-CNF) with an average width of 5.1 ± 0.2 nm were obtained after subsequential ultrasonication. The peak heat release rate of P-CNF was reduced by 89.1 % compared with unmodified softwood pulp, and the limiting oxygen index value increased to 44.8 %. Furthermore, the fabricated P-CNF based film demonstrated good self-extinguishing behavior in both horizontal and vertical combustion tests, high transparency (visible-light transmittance above 80 %) and robust mechanical properties. This developed RDES system provided a new and sustainable route to prepare intrinsically flame-retardant nanocellulose, which may have potential applications in many fields such as thermal insulation, and electronics.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122931"},"PeriodicalIF":10.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharides from Lycium barbarum, yam, and sunflower ameliorate colitis in a structure and intrinsic flora-dependent manner 枸杞、山药和向日葵多糖以结构和内在菌群依赖的方式改善结肠炎

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122905

Shanshan Zhang , Ruchen Zhou , Xiaoran Xie , Shanshan Xiong , Lixiang Li , Yanqing Li

{"title":"Polysaccharides from Lycium barbarum, yam, and sunflower ameliorate colitis in a structure and intrinsic flora-dependent manner","authors":"Shanshan Zhang , Ruchen Zhou , Xiaoran Xie , Shanshan Xiong , Lixiang Li , Yanqing Li","doi":"10.1016/j.carbpol.2024.122905","DOIUrl":"10.1016/j.carbpol.2024.122905","url":null,"abstract":"<div><div>Polysaccharides have been suggested to ameliorate metabolic diseases. However, their differential colitis-mitigating effects in mouse models with different colony structures remain poorly understood. Therefore, this study investigated the effects of polysaccharides from <em>Lycium barbarum</em> (LBP), sunflower (SP), and yam (YP) on colitis in C57BL/6 J (B6) mice born via vaginal delivery (VD) and in both caesarean section (CS)- and VD-born Institute of Cancer Research (ICR) mice. LBP was mainly composed of glucose (30.2 %), galactose (27.5 %), and arabinose (26.9 %). The main components of SP and YP were galacturonic acid (75.8 %) and glucose (98.1 %), respectively. Interestingly, LBP effectively alleviated body weight loss, reduced inflammatory cytokine levels, and restored intestinal barrier function in all three mouse models. Moreover, LBP decreased the abundance of <em>norank_f__norank_o__Clostridia_UCG-014, Coriobacteriaceae_UCG-002, and norank_f_Eubacterium_coprostanoligenes_group</em> in B6 mice, and the abundance of these genera positively correlated with pro-inflammatory cytokine levels. LBP increased the abundance of <em>Lactobacillus</em>, which was positively correlated with the levels of the protective factor, <em>IL-10</em>, in CS-born ICR mice. Collectively, our study suggests the potential application of LBP in the treatment of ulcerative colitis. We also provide an alternative method for restoring intestinal homeostasis in CS-born offspring.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122905"},"PeriodicalIF":10.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heparin-azithromycin microparticles show anti-inflammatory effects and inhibit SARS-CoV-2 and bacterial pathogens associated to lung infections 肝素-阿奇霉素微粒具有抗炎作用，可抑制与肺部感染相关的 SARS-CoV-2 和细菌病原体

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122930

Brayan J. Anaya , Davide D'Angelo , Ruggero Bettini , Gracia Molina , Amadeo Sanz-Perez , María Auxiliadora Dea-Ayuela , Carolina Galiana , Carmina Rodríguez , Diego F. Tirado , Aikaterini Lalatsa , Elena González-Burgos , Dolores R. Serrano

{"title":"Heparin-azithromycin microparticles show anti-inflammatory effects and inhibit SARS-CoV-2 and bacterial pathogens associated to lung infections","authors":"Brayan J. Anaya , Davide D'Angelo , Ruggero Bettini , Gracia Molina , Amadeo Sanz-Perez , María Auxiliadora Dea-Ayuela , Carolina Galiana , Carmina Rodríguez , Diego F. Tirado , Aikaterini Lalatsa , Elena González-Burgos , Dolores R. Serrano","doi":"10.1016/j.carbpol.2024.122930","DOIUrl":"10.1016/j.carbpol.2024.122930","url":null,"abstract":"<div><div>Pulmonary infections are a leading cause of morbidity and mortality worldwide, a situation exacerbated by the COVID-19. Azithromycin (AZM) is used orally to treat pulmonary infections due to its ability to accumulate in lung tissues and immune cells after oral administration. Sulfated polysaccharides, such as heparin, are known to inhibit SARS-CoV-2 entry. This study presents a novel approach focused on developing a dry powder inhaler of AZM-loaded microparticles composed of either heparin or its derivatives. The microparticle formulations exhibited potent antiviral activity against SARS-CoV-2 (IC50 ≤ 95 nM) while retaining superior antibacterial efficacy against <em>Streptococcus pneumoniae</em> and <em>Pseudomonas aeruginosa</em> compared to free AZM (MIC ≤15 μg/mL). Importantly, at bactericidal concentrations, no cytotoxic effects were observed on mammalian cells, including Calu-3 cells and red blood cells. The formulations demonstrated effective alveolar aerodynamic deposition (MMAD ranging from 1 μm to 3 μm) with a Fine Particle Fraction below 5 μm close to 50 %. Adopting a conservative estimate of 20 mL for the pulmonary epithelial lining fluid volume in healthy adults, efficacious local concentrations of sulfated polysaccharides and AZM would be delivered to the lung using this multifaceted strategy which holds promise for the treatment of bacterial pulmonary infections associated with COVID-19.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122930"},"PeriodicalIF":10.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatography-free synthesis of 2A,2B-disulfonated β-cyclodextrin for regiospecific di-substitution 用于区域特异性二取代的 2A,2B-二磺化 β-环糊精的无色谱法合成

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122926

Christopher J. Hobbs, Vít Novotný, Michal Řezanka

引用次数: 0

Pullulan dialdehyde cross-linked dual-action adhesive with high adhesion to lung tissue and the capability of pH-responsive drug release 具有肺组织高粘附性和 pH 值响应药物释放能力的普鲁兰二甲醛交联双效粘合剂

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122906

Ke Zhang , Hui Huang , Yilin Zhao , Qinghao Zhen , Dongjian Shi , Jinghua Chen , Xi Chen

{"title":"Pullulan dialdehyde cross-linked dual-action adhesive with high adhesion to lung tissue and the capability of pH-responsive drug release","authors":"Ke Zhang , Hui Huang , Yilin Zhao , Qinghao Zhen , Dongjian Shi , Jinghua Chen , Xi Chen","doi":"10.1016/j.carbpol.2024.122906","DOIUrl":"10.1016/j.carbpol.2024.122906","url":null,"abstract":"<div><div>To address the main challenges for thoracoscopic lung cancer surgery, including persistent pulmonary air leaks and cancer recurrence, this study developed an in-situ adhesive that can effectively adhere to the lung and release the anticancer drug in response to pH. The adhesive was formulated using hydrophobically modified cold-water fish skin gelatin (hm-CFG) and cross-linking agent pullulan dialdehyde (PDA), in which succinic dihydrazide-modified doxorubicin (SDH-DOX) can be incorporated. Utilizing PDA could improve both cohesion and interfacial adhesion, while also offering drug-loading sites through the aldehyde groups that were not involved in cross-linking. The optimal adhesive formulation was 9C10-CFG/PDA (30 w/v% 9 mol% decanal modified CFG/20 w/v% PDA). The 9C10-CFG/PDA adhesive exhibited suitable cohesive strength, good mechanical flexibility (tensile strain over 170 %), and strong interface adhesion. The burst strength of 9C10-CFG/PDA adhesive (131.5 ± 22.2 mm Hg) was almost 6-fold higher than that of commercial fibrin sealant. In a rat pneumothorax model, 9C10-CFG/PDA adhesive displayed favorable wound-sealing properties, as evidenced by CT imaging and restored rat behavior. When combined with the anticancer drug, SDH-DOX@Adhesive could release the drug in response to pH more gradually than DOX@Adhesive. This dual-action adhesive is anticipated to mitigate post-surgical occurrences of lung air leaks and cancer recurrence.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122906"},"PeriodicalIF":10.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-directions mechanical strength and high-barrier mechanisms of cellulose nanocrystal- based hybrids reinforced packaging with nacre-mimetic structure 基于纤维素纳米晶的双向机械强度和高阻隔机理--仿珍珠质结构的混合加固包装

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-28 DOI: 10.1016/j.carbpol.2024.122910

Amare Worku Alebachew , Somia Yassin Hussain Abdalkarim , Jingli Zhu , Shuping Wu , Yuanhua Zhang , Hou-Yong Yu , Khaydar E. Yunusov

{"title":"Two-directions mechanical strength and high-barrier mechanisms of cellulose nanocrystal- based hybrids reinforced packaging with nacre-mimetic structure","authors":"Amare Worku Alebachew , Somia Yassin Hussain Abdalkarim , Jingli Zhu , Shuping Wu , Yuanhua Zhang , Hou-Yong Yu , Khaydar E. Yunusov","doi":"10.1016/j.carbpol.2024.122910","DOIUrl":"10.1016/j.carbpol.2024.122910","url":null,"abstract":"<div><div>This work represents a new composite film with a nacre-mimetic structure through the alignment of hybrids comprising cellulose nanocrystals and ball-milled boron nitride (CNC-BNNS), within polypropylene carbonate (PPC) endowed with various properties. The impact of CNC-BNNS hybrids on mechanical strength mechanisms was evaluated under two-directional forces, marking the first such assessment. Using a solution casting approach, incorporating 5 % CNC-BNNS improved tensile strength by 67.6 % longitudinally and 4 % in the cross-sectional direction of the prepared PPC composite. Adding 5 %, CNC-BNNS increased the maximum degradation temperature by 12.1 °C and the glass transition temperature by 6.8 °C, as anticipated. Through synergies between CNC-BNNS and PPC, composite film with 15 % CNC-BNNS displayed enhanced barrier properties, notably reducing oxygen permeability (OP) and water vapor permeability (WVP) by 26.1 % and 90.9 %. Consequently, strawberries and blueberries packaged with these films saw extended shelf lives of up to 6 days. Furthermore, the degradation mechanisms of the composite in natural soil were investigated to assure the environmental issue, revealing a fast degradation rate of 44.5 % over 90 days. This study proposes a sustainable method for producing high-performance composite materials with antioxidant, liquid leakage resistance, and UV resistance, making them ideal for food packaging.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122910"},"PeriodicalIF":10.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of polysaccharide and cell wall structure in Aspergillus nidulans and Aspergillus fumigatus by solid-state NMR 通过固态核磁共振对比分析裸曲霉和烟曲霉的多糖和细胞壁结构

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-26 DOI: 10.1016/j.carbpol.2024.122907

Isha Gautam , Jayasubba Reddy Yarava , Yifan Xu , Reina Li , Faith J. Scott , Frederic Mentink-Vigier , Michelle Momany , Jean-Paul Latgé , Tuo Wang

{"title":"Comparative analysis of polysaccharide and cell wall structure in Aspergillus nidulans and Aspergillus fumigatus by solid-state NMR","authors":"Isha Gautam , Jayasubba Reddy Yarava , Yifan Xu , Reina Li , Faith J. Scott , Frederic Mentink-Vigier , Michelle Momany , Jean-Paul Latgé , Tuo Wang","doi":"10.1016/j.carbpol.2024.122907","DOIUrl":"10.1016/j.carbpol.2024.122907","url":null,"abstract":"<div><div>Invasive aspergillosis poses a significant threat to immunocompromised patients, leading to high mortality rates associated with these infections. Targeting the biosynthesis of cell wall carbohydrates is a promising strategy for antifungal drug development and will be advanced by a molecular-level understanding of the native structures of polysaccharides within their cellular context. Solid-state NMR spectroscopy has recently provided detailed insights into the cell wall organization of <em>Aspergillus fumigatus</em>, but genetic and biochemical evidence highlights species-specific differences among <em>Aspergillus</em> species. In this study, we employed a combination of <sup>13</sup>C, <sup>15</sup>N, and <sup>1</sup>H-detection solid-state NMR, supplemented by Dynamic Nuclear Polarization (DNP), to compare the structural organization of cell wall polymers and their assembly in the cell walls of <em>A. fumigatus</em> and <em>A. nidulans</em>, both of which are key model organisms and human pathogens. The two species exhibited a similar rigid core architecture, consisting of chitin, α-glucan, and β-glucan, which contributed to comparable cell wall properties, including polymer dynamics, water retention, and supramolecular organization. However, differences were observed in the chitin, galactosaminogalactan, protein, and lipid content, as well as in the dynamics of galactomannan and the structure of the glucan matrix.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122907"},"PeriodicalIF":10.7,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation analysis of starch molecular structure and film properties via rearrangements of glycosidic linkages by 1,4-α-glucan branching enzyme 通过 1,4-α-葡聚糖分支酶的糖苷键重排分析淀粉分子结构与薄膜特性的相关性

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-26 DOI: 10.1016/j.carbpol.2024.122908

Yilin Dong , Zhaofeng Li , Haocun Kong , Xiaofeng Ban , Zhengbiao Gu , Hao Zhang , Yan Hong , Li Cheng , Caiming Li

{"title":"Correlation analysis of starch molecular structure and film properties via rearrangements of glycosidic linkages by 1,4-α-glucan branching enzyme","authors":"Yilin Dong , Zhaofeng Li , Haocun Kong , Xiaofeng Ban , Zhengbiao Gu , Hao Zhang , Yan Hong , Li Cheng , Caiming Li","doi":"10.1016/j.carbpol.2024.122908","DOIUrl":"10.1016/j.carbpol.2024.122908","url":null,"abstract":"<div><div>The functional characteristics of starch films are significantly influenced by the amylose content and the distribution of the amylopectin chain length. This work used 1,4-α-glucan branching enzyme to molecularly reconstruct corn, pea, and cassava starch in order to examine the association. Films made of both natural and enzyme-modified starch were produced using the casting method. The study investigated the variations in starch films properties and explored the relationship between starch molecular structure and film qualities by correlation analysis. The results showed a significant positive connection (<em>r</em> = 0.954) between the tensile strength and amylose content, as well as a positive correlation (<em>r</em> = 0.939) between the A chains and the elongation at break. The average chain length (<em>r</em> = 0.932) and amylose content (<em>r</em> = 0.902) showed a positive correlation with the degradation temperature, whereas the amylose content (<em>r</em> = −0.946) showed an adverse correlation with the transparency. The B3 chain (<em>r</em> = 0.851) and the average chain length (<em>r</em> = 0.839) both exhibited a positive connection with its contact angle. As a result, our study thoroughly assesses how starch structure affects the characteristics of starch films and offers a fundamental modification pathway for the development of new application areas.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122908"},"PeriodicalIF":10.7,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of super stretchability, rapid self-healing, and self-adhesion hydrogel based on starch for wearable strain sensors 设计基于淀粉的超伸展性、快速自愈合和自粘合水凝胶，用于可穿戴应变传感器

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-24 DOI: 10.1016/j.carbpol.2024.122858

Yanyan Li , Xin Wen , Xiaoru Li , Muhammad Zahid , Hongliang Wang , Jian Zhang

{"title":"Design of super stretchability, rapid self-healing, and self-adhesion hydrogel based on starch for wearable strain sensors","authors":"Yanyan Li , Xin Wen , Xiaoru Li , Muhammad Zahid , Hongliang Wang , Jian Zhang","doi":"10.1016/j.carbpol.2024.122858","DOIUrl":"10.1016/j.carbpol.2024.122858","url":null,"abstract":"<div><div>Since hydrogels are conductive, easily engineered, and sufficiently flexible to imitate the mechanical properties of human skin, they are seen as potential options for wearable strain sensors. However, it is still a great challenge to prepare a hydrogel through simple and straightforward methods that integrate excellent stretchability, ionic conductivity, toughness, self-adhesion, and self-healing. Herein, an acrylamide/3-acrylamide phenylboronic acid cross-linked network is modified to produce a semi-interpenetrating cross-linked hydrogel in just one easy step by adding starch. The prepared hydrogel contains dynamic boronic ester bonds and hydrogen bonds, which endow the exceptional stretchability (5769–13,976 %, 20–50 wt%), ideal transmittance (>90 %), self-adhesiveness (0.636 ± 0.060 kPa, 30 wt%), and self-healing properties. Notably, the self-healing process is completed instantly, achieving a healing strength of up to 81.21 %. Additionally, the aforementioned hydrogel exhibits a broad working strain range (≈ 500 %) and high sensitivity (gauge factor = 1.99) as a strain sensor, allowing it to record and track human actions precisely. This work provides a novel approach to synthesizing hydrogels with optimal overall mechanical characteristics, with the potential to facilitate the development of wearable strain sensing system based on hydrogels for real-world applications.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122858"},"PeriodicalIF":10.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editing of the soluble starch synthase gene MeSSIII-1 enhanced the amylose and resistant starch contents in cassava 编辑可溶性淀粉合成酶基因 MeSSIII-1 可提高木薯中直链淀粉和抗性淀粉的含量

IF 10.7 1区化学

Carbohydrate Polymers Pub Date : 2024-10-24 DOI: 10.1016/j.carbpol.2024.122903

Xiaohua Lu , Yajie Wang , Xinghou Zhen , Yannian Che , Hui Yu , Yujian Ge , Xiangwen Wang , Ruimei Li , Mengting Geng , Bin Zhou , Jiao Liu , Jianchun Guo , Yuan Yao

{"title":"Editing of the soluble starch synthase gene MeSSIII-1 enhanced the amylose and resistant starch contents in cassava","authors":"Xiaohua Lu , Yajie Wang , Xinghou Zhen , Yannian Che , Hui Yu , Yujian Ge , Xiangwen Wang , Ruimei Li , Mengting Geng , Bin Zhou , Jiao Liu , Jianchun Guo , Yuan Yao","doi":"10.1016/j.carbpol.2024.122903","DOIUrl":"10.1016/j.carbpol.2024.122903","url":null,"abstract":"<div><div>Foods with high amylose and resistant starch (RS) contents have great potential to enhance human health. In this study, cassava soluble starch synthase <em>MeSSIII-1</em> gene mutants were generated using CRISPR/Cas9 system. The results showed that the storage roots of <em>messiii-1</em> mutants had higher contents of amylose, RS, and total starch than those in CK<em>.</em> The rates of small and large-sized starch granules were increased. Additionally, amylopectin starch in <em>messiii-1</em> mutants had a higher proportion of medium- and long- chains, and a lower proportion of short-chains than those in CK. The onset, peak, and conclusion temperatures of starch gelatinization in <em>messiii-1</em> mutants were significantly lower than those in CK, and the peak viscosity, trough viscosity and final viscosity all increased. <em>MeSSIII-1</em> mutation could increase the contents of sucrose, glucose, and fructose in cassava storage roots. We hypothesize that these soluble sugars serve a dual role: they provide the necessary carbon source for starch synthesis and act as sugar signals to trigger the transcriptional reprogramming of genes involved in starch biosynthesis. This process results in a collective enhancement of amylose, RS, and total starch contents, accompanied by changes in starch granule morphology, fine structure, and physicochemical properties.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122903"},"PeriodicalIF":10.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0