Biomass & Bioenergy最新文献_第7页

Study of phenoxyethanol-citric acid biphasic pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis 苯氧乙醇-柠檬酸双相预处理蔗渣促进酶解的研究

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-13 DOI: 10.1016/j.biombioe.2025.108388

Jili Liao , Ruize Wang , Xin Li , Ruolin Li , Danna Xie , Xiaoxue Zhao , Jing Wang , Caoxing Huang

{"title":"Study of phenoxyethanol-citric acid biphasic pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis","authors":"Jili Liao , Ruize Wang , Xin Li , Ruolin Li , Danna Xie , Xiaoxue Zhao , Jing Wang , Caoxing Huang","doi":"10.1016/j.biombioe.2025.108388","DOIUrl":"10.1016/j.biombioe.2025.108388","url":null,"abstract":"<div><div>In this study, a green, lignin-selective, and recyclable solvent of phenoxyethanol (EPH) combined with citric acid (CA) was employed as a biphasic pretreatment system to effectively deconstruct the recalcitrant structure of sugarcane bagasse (SCB) to enhance its enzymatic digestibility. The results showed that the removal of lignin was 81.9 % and the enzymatic hydrolysis yield of SCB reached 73.9 % under the pretreatment at 170 °C with 10.0 % CA. The physicochemical changes of the SCB after the pretreatment showed that the lignin removal, hemicellulose removal, the hydrophobicity, and accessibility of the pretreated SCB were positively correlated. The interactions between lignin and other components in the phenoxyethanol-citric acid (PECA) system were analyzed through molecular dynamics (MD) simulations combined with density functional theory (DFT) calculations. It is found that the lignin molecule can interact with EPH and water to preferentially solubilize in the EPH phase due to the formed high interaction energy and increased hydrogen bond formation. In the lignin-EPH system, the addition of CA increased the number of hydrogen bonds and enhanced the interaction between lignin and the three components, which was conducive to achieving a higher degree of delignification for improved enzymatic hydrolysis.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"204 ","pages":"Article 108388"},"PeriodicalIF":5.8,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of hydrochar produced from hydrothermal liquefaction of pipeline-transported softwood biomass 管道输送软木生物质水热液化制烃类的生产与表征

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-12 DOI: 10.1016/j.biombioe.2025.108339

Omex Mohan, Olugbenga Abiola Fakayode, Amit Kumar

{"title":"Production and characterization of hydrochar produced from hydrothermal liquefaction of pipeline-transported softwood biomass","authors":"Omex Mohan, Olugbenga Abiola Fakayode, Amit Kumar","doi":"10.1016/j.biombioe.2025.108339","DOIUrl":"10.1016/j.biombioe.2025.108339","url":null,"abstract":"<div><div>This study investigates hydrochar production from hydrothermal liquefaction (HTL) of pipeline-transported softwood biomass. Pipelining biomass using water as a carrier fluid offers a cost-effective alternative to truck transport, and its integration with HTL for hydrochar production is relatively unexplored. The objective of this study was to evaluate the effect of process parameters on HTL of pipelined biomass and characterize the resulting hydrochar for various applications. The yield of hydrochar produced from pipelined biomass, at the optimal process conditions, was 13.22 % (oven-dry mass basis), with 19.31 % of energy recovered in hydrochar. The hydrochar was characterized as a black, fragile, mildly acidic carbon material with a dry bulk density of 0.142 g/cm<sup>3</sup>, improved carbon content, increased surface area (9.27 m<sup>2</sup>/g), altered pore morphology, and a graphitized, amorphous structure, reflecting structural and compositional changes from the HTL process. Pipelining was found to have minimal impact on the lignocellulosic composition and particle size of the softwood biomass, but it resulted in the leaching of inorganic elements, leading to a reduction in ash content in the hydrochar. The characterization results suggest the hydrochar is suitable for soil amendment and solid fuel applications; its low surface area hinders its use as an adsorbent or for energy storage. This study thus demonstrates the potential of integrating hydro-transportation with HTL, offering a pathway to recover, manage, and use hydrochar for various applications.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108339"},"PeriodicalIF":5.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar and its impact on the carbon cycle 生物炭及其对碳循环的影响

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-12 DOI: 10.1016/j.biombioe.2025.108365

David Chiaramonti , Franco Berruti , Johannes Lehmann , Ondrej Masek , Henrik Ingermann Petersen , Manuel Garcia Perez , Hamed Sanei , Francesco Primo Vaccari

引用次数: 0

Valorization of corn straw digestate compost via hydrothermal humification for sustainable humic acid production 热液腐殖酸对玉米秸秆堆肥腐殖酸可持续生产的影响

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-12 DOI: 10.1016/j.biombioe.2025.108363

Ming Wang, Hao Peng, Qingjie Wang, Manman Wang, Jinxia Fan, Shuang Liu

{"title":"Valorization of corn straw digestate compost via hydrothermal humification for sustainable humic acid production","authors":"Ming Wang, Hao Peng, Qingjie Wang, Manman Wang, Jinxia Fan, Shuang Liu","doi":"10.1016/j.biombioe.2025.108363","DOIUrl":"10.1016/j.biombioe.2025.108363","url":null,"abstract":"<div><div>Corn straw (CS) digestate, rich in lignocellulose, generates substantial residues after anaerobic digestion. This study explored the hydrothermal humification (HTH) characteristics of CS digestate (CSD) and CSD compost (CSDC) from a continuous-feed biogas project. After HTH at 180 °C for 4 h, the humic-like fluorescence signals and humic acid (HA) yield of CSDC were significantly higher than those of CS and CSD, with a maximum HA yield of 31.66 wt%, 3.1 and 2.6 times greater, respectively. Lignin degradation during HTH (removal rates of 71.78 %–87.58 %) promoted HA formation. The HA from CSDC exhibited a composition and morphology more similar to lignite HA, featuring higher aliphatic carbon and surface oxygen content, closely resembling black soil HA. These properties endowed it with enhanced aromaticity, ion-exchange capacity, and biodegradability. A germination test showed that 0.001 % HA treatment achieved a germination index of 132 %. Thus, CSDC is an excellent feedstock for artificial HA production, supporting the high-value utilization of CS digestate.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108363"},"PeriodicalIF":5.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impregnation of bio-oil to homogenous biochar diminishes activity in volatiles-char interactions during pyrolysis of lotus seedpod 生物油浸渍均质生物炭降低了莲荚热解过程中挥发物-炭相互作用的活性

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-12 DOI: 10.1016/j.biombioe.2025.108364

Kai Sun , Lijun Zhang , Chao Li , Guozhu Chen , Xun Hu

{"title":"Impregnation of bio-oil to homogenous biochar diminishes activity in volatiles-char interactions during pyrolysis of lotus seedpod","authors":"Kai Sun , Lijun Zhang , Chao Li , Guozhu Chen , Xun Hu","doi":"10.1016/j.biombioe.2025.108364","DOIUrl":"10.1016/j.biombioe.2025.108364","url":null,"abstract":"<div><div>Volatiles-char interactions in pyrolysis of biomass have been well documented, and increase of abundance of oxygen-containing species on surface of biochar might enhance activity for reacting with volatiles. This was investigated herein by modification of bio-oil to the homogenous biochar from pyrolysis of lotus seedpod followed by subsequent thermal treatment at 600 °C to enhance presence of aliphatic organics on biochar. The aim was to increase activity of the modified biochar for interacting with volatiles. Contrary to expectations, the pure biochar that was not modified by bio-oil effectively catalyzed cracking/gasification of volatiles to gases. The results were that the pyrolysis of lotus seedpod with pure biochar formed more gases (40.1 % versus 24.3 %) and lower yields of bio-oil (27.3 % versus 40.1 %) at 600 °C, compared with that of biochar modified with bio-oil. The biochar modified with bio-oil, however, showed negligible activity for cracking, but did catalyze condensation reactions to form more tar (5.4 % versus 3.7 % from the control experiment). The impregnation of bio-oil to biochar with subsequent calcination at 600 °C did not increase abundance of oxygen-containing aliphatics but made modified biochar more carbon-rich and oxygen-poor from cracking of organics in bio-oil. Such a loss of oxygen-containing species “deactivated” biochar. Dynamic change of reactivity of nascent biochar with volatiles with progress of pyrolysis also took place from losing oxygen species on surface of biochar. This made biochar more aromatic, thermally stable, hydrophobic but produced more volatiles while less gases, along with deactivation of biochar.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108364"},"PeriodicalIF":5.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental examination of reactive surface areas and particle sizes configuration effects on hydrothermally pretreated biomass for maximal accessibility of constituent compounds 活性表面积和颗粒大小对水热预处理生物质组分化合物最大可及性影响的实验研究

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-11 DOI: 10.1016/j.biombioe.2025.108362

Emmanuel Chinonye Mgbemene , Erkay Ozgor , Emrah Ahmet Erkurt

{"title":"Experimental examination of reactive surface areas and particle sizes configuration effects on hydrothermally pretreated biomass for maximal accessibility of constituent compounds","authors":"Emmanuel Chinonye Mgbemene , Erkay Ozgor , Emrah Ahmet Erkurt","doi":"10.1016/j.biombioe.2025.108362","DOIUrl":"10.1016/j.biombioe.2025.108362","url":null,"abstract":"<div><div>The experimental frameworks explored how to maximally access constituent biocompounds, sequel to hydrothermal pretreatment, subjective to particle size configuration and reactive surface areas. Particle size ranges of samples were hypothesized as crucial parameters required for the evaluation of hydrothermal pretreatment efficiency. Depolymerization, degradation and denaturation of petioles biomass cell walls were achieved through hydrothermal pretreatment done by autoclaving at 121 °C and 1.5atm for 2 h. The conversion efficiencies of the hydrothermally pretreated samples were instrumentally determined using Mastersizer 2000 analyzer, FTIR and XRD analyses. It demonstrated an evolutionary approach in the exploration of reactive surface areas, particle sizes configuration and pretreatment severity. The experimental samples 1, 2 and 3 exhibited diverse levels of transformations as a result of pretreatment and particle size differences. Experimental data showed that samples of smaller particle size ranges (0–0.074 mm) displayed access to more reactive surface areas than the higher particle size ranges (0.150 mm and 0.335 mm sieves). Untreated sample 4 showed minimal reactive surface areas and poor accessibility of constituent compounds. The novelty was partly demonstrated in particle sizes configuration analysis with reactive surface areas targeted at maximizing pretreatment efficiency. This approach is a prerequisite which aims to advance the production of biofuel, bioenergy and other bioproducts. It is significant in biorefinery design and optimization through utilization of recommendable particle size ranges or thresholds that guarantee optimal accessibility and low energy consumption.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108362"},"PeriodicalIF":5.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coupled effect of pyrolysis temperature and HNO3 pretreatment on sugarcane bagasse fast pyrolysis for levoglucosan production 热解温度与HNO3预处理对蔗渣快速热解制备左旋葡聚糖的耦合影响

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-11 DOI: 10.1016/j.biombioe.2025.108372

Geraldo Ferreira David , Rodolfo Roberto Moreno-Parra , Victor Haber Perez , Thays da Costa Silveira , Euripedes Garcia Silveira Junior , Oselys Rodriguez Justo , Manuel Garcia-Perez

{"title":"Coupled effect of pyrolysis temperature and HNO3 pretreatment on sugarcane bagasse fast pyrolysis for levoglucosan production","authors":"Geraldo Ferreira David , Rodolfo Roberto Moreno-Parra , Victor Haber Perez , Thays da Costa Silveira , Euripedes Garcia Silveira Junior , Oselys Rodriguez Justo , Manuel Garcia-Perez","doi":"10.1016/j.biombioe.2025.108372","DOIUrl":"10.1016/j.biombioe.2025.108372","url":null,"abstract":"<div><div>This work aimed to evaluate the potential of HNO<sub>3</sub> (0.1 wt%) in the pretreatment of sugarcane bagasse as a strategy to intensify levoglucosan production, before subjecting it to fast pyrolysis under different temperature conditions. The experiments were conducted in a pyroprobe reactor coupled to a GC/MS at temperatures ranging from 350 to 600 °C, with 50 °C intervals. Thus, in addition to the impact of nitric acid on levoglucosan yield, the formation profile of other pyrolytic derivatives during the thermochemical conversion of biomass is reported. The best results regarding levoglucosan yield were obtained when pyrolysis was performed at 450 °C, resulting in approximately twice the amount of levoglucosan obtained from sugarcane bagasse after treating the biomass with acid compared to the control (untreated biomass), while the profiles of the other pyrolytic derivatives identified showed different and lower behaviors after acid washing of the biomass. The observed effect may be due not only to the removal of alkali and alkaline earth metals, whose catalytic activity influences the pyrolytic pathways, but also to the probable partial removal of lignin and/or hemicellulose fractions by the acid washing. On the other hand, despite the observed increase in levoglucosan formation, the results obtained with HNO<sub>3</sub> at 0.1 % by biomass weight do not seem to be a good alternative for the intensification of the target compound, when compared with other acids used in washing/passivation procedures of sugarcane bagasse under similar experimental conditions.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108372"},"PeriodicalIF":5.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm cultivation and air blow harvesting of Navicula incerta on PVDF hollow fiber membrane with different biocoatings 不同生物涂层的PVDF中空纤维膜上水蛭的生物膜培养及吹收

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-11 DOI: 10.1016/j.biombioe.2025.108373

Jia Xin Yap , C.P. Leo , Derek Juinn Chieh Chan , Nazlina Haiza Mohd Yasin

{"title":"Biofilm cultivation and air blow harvesting of Navicula incerta on PVDF hollow fiber membrane with different biocoatings","authors":"Jia Xin Yap , C.P. Leo , Derek Juinn Chieh Chan , Nazlina Haiza Mohd Yasin","doi":"10.1016/j.biombioe.2025.108373","DOIUrl":"10.1016/j.biombioe.2025.108373","url":null,"abstract":"<div><div>Biofilm cultivation reduced the harvesting and drying costs in microalgae agronomy. Although microalgae can be cultivated and harvested using the porous membrane, the surface properties restrict cell adhesion and removal. In this study, the porous polyvinylidene fluoride (PVDF) hollow fiber membranes with different biocoatings were used to grow <em>Navicula incerta</em> at the air-liquid interface. Fourier transform infrared spectra showed the characteristic peaks of the coating materials, while the water contact angle measurement revealed that bovine serum (BSA) increased surface hydrophobicity most significantly. The zeta potential further proved that BSA also increased the adhesion energy, leading to the highest growth rate of <em>N. incerta</em> of 67.3 % but a slight drop in air blow harvesting efficiency of 78.73 %. BSA boosted astaxanthin accumulation in microalgae up to 4.77 μg/mL, enhancing cell proliferation. SEM images displayed microalgae cells with extracellular organic matter attached to different biocoatings. The membrane porosity was not significantly affected even after cultivation and harvesting.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108373"},"PeriodicalIF":5.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the synergistic effect in the co-pyrolysis between chicken manure biogas residue and low-density polyethylene 鸡粪沼气渣与低密度聚乙烯共热解协同效应研究

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-11 DOI: 10.1016/j.biombioe.2025.108368

Tie Wang, Kaili Xu, Xiwen Yao, Jishuo Li, Haodong Zhou

{"title":"Study on the synergistic effect in the co-pyrolysis between chicken manure biogas residue and low-density polyethylene","authors":"Tie Wang, Kaili Xu, Xiwen Yao, Jishuo Li, Haodong Zhou","doi":"10.1016/j.biombioe.2025.108368","DOIUrl":"10.1016/j.biombioe.2025.108368","url":null,"abstract":"<div><div>The synergistic effect in the co-pyrolysis between chicken manure biogas residue (CMBR) and low-density polyethylene (LDPE) was revealed. The weight loss and kinetics during co-pyrolysis were characterized using a synchronous thermal analyzer. The effects of mixing ratio and pyrolysis temperature on the characteristics of the three-phase products of co-pyrolysis were analyzed using a tube furnace. The results demonstrated an increase in mass loss rate (<em>M</em><sub>f</sub>) and comprehensive pyrolysis index (<em>CPI</em>) and a reduction in activation energy compared to theoretical calculations, confirming the presence of synergistic effect in the co-pyrolysis between the two materials. Positive synergistic effect was the most pronounced at the mixing ratio of 1:1. Co-pyrolysis inhibited bio-oil production and reduced the O/C ratio of bio-oil; it also precipitated the release of pyrolysis gas and increased the yields of CH<sub>4</sub> and hydrocarbon gas (C<sub>n</sub>H<sub>m</sub>). Co-pyrolysis reduced the content of nitrogenated organic compounds in bio-char, enhanced its aromaticity and altered the pore structure on the bio-char surface. At higher pyrolysis temperatures, co-pyrolysis bio-oil further transformed into small-molecule gases, leading to an increase in the yields of CO and H<sub>2</sub>.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108368"},"PeriodicalIF":5.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AHP-GRA based multi-criteria optimization of sorghum-sudangrass hybrid and alfalfa silage mixtures for enhanced quality and methane reduction 基于AHP-GRA的高粱-苏草和苜蓿青贮混合料品质优化及甲烷减量研究

IF 5.8 2区生物学

Biomass & Bioenergy Pub Date : 2025-09-11 DOI: 10.1016/j.biombioe.2025.108360

Ekin Sucu , Asli Ergenekon Arslan , Erdem Gulumser , Oguz Arslan

{"title":"AHP-GRA based multi-criteria optimization of sorghum-sudangrass hybrid and alfalfa silage mixtures for enhanced quality and methane reduction","authors":"Ekin Sucu , Asli Ergenekon Arslan , Erdem Gulumser , Oguz Arslan","doi":"10.1016/j.biombioe.2025.108360","DOIUrl":"10.1016/j.biombioe.2025.108360","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of ensiling sorghum-sudangrass hybrid (SS) and alfalfa at varying ratios on silage quality, ruminal fermentation characteristics, and <em>anti</em>-methanogenic potential. Silage mixtures containing SS and alfalfa at ratios of 100:0, 75:25, 50:50, 25:75, and 0:100 (wet weight basis) were ensiled for 45 days. As the proportion of SS decreased, there was a significant linear increase in crude protein, mineral content (Ca, Mg, P), and secondary metabolites (condensed tannins and phenolics) (P < 0.01), along with a decrease in dry matter (DM), Flieg score, acid detergent fiber (ADF), neutral detergent fiber (NDF), lactate, and butyrate concentrations (P < 0.01). Increasing alfalfa inclusion linearly reduced 24-h in vitro gas production (P < 0.01), but had no effect on cumulative gas production at 96 h. To address the complex interrelationships among fermentation and nutritional parameters, grey relational analysis (GRA) was performed. The Analytic Hierarchy Process (AHP) was integrated into GRA to assign expert-weighted significance levels to each parameter. Based on the AHP-GRA model, the 75:25 SS:alfalfa mixture was identified as the optimal silage combination, displaying the lowest rumen pH, acetate, propionate, CO<sub>2</sub>, and CH<sub>4</sub> concentrations (P < 0.05), and reduced proteolysis. These findings suggest that co-ensiling SS and alfalfa at a 75:25 ratio improves silage quality and mitigates enteric CH<sub>4</sub> emissions. However, further in vivo validation is necessary to confirm these effects under practical feeding conditions.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"203 ","pages":"Article 108360"},"PeriodicalIF":5.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0