苯氧乙醇-柠檬酸双相预处理蔗渣促进酶解的研究

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING

Biomass & Bioenergy Pub Date : 2025-09-13 DOI:10.1016/j.biombioe.2025.108388

Jili Liao , Ruize Wang , Xin Li , Ruolin Li , Danna Xie , Xiaoxue Zhao , Jing Wang , Caoxing Huang

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引用次数: 0

摘要

本研究采用绿色、木质素选择性、可回收的溶剂苯氧乙醇（EPH）与柠檬酸（CA）结合作为双相预处理体系，有效地解构甘蔗甘蔗渣（SCB）的顽固性结构，提高其酶解率。结果表明，在170℃、10.0% CA条件下，木质素的去除率为81.9%，木质素的酶解率为73.9%。预处理后木质素的理化变化表明，预处理后木质素的去除率、半纤维素的去除率、疏水性和可及性呈正相关。通过分子动力学（MD）模拟和密度泛函理论（DFT）计算，分析了苯氧乙醇-柠檬酸（PECA）体系中木质素与其他组分之间的相互作用。结果表明，木质素分子与EPH和水相互作用，形成较高的相互作用能，促进氢键形成，优先溶于EPH相中。在木质素- eph体系中，CA的加入增加了氢键的数量，增强了木质素与三组分之间的相互作用，有利于实现更高程度的脱木质素，从而改善酶解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Study of phenoxyethanol-citric acid biphasic pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis

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Study of phenoxyethanol-citric acid biphasic pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis

In this study, a green, lignin-selective, and recyclable solvent of phenoxyethanol (EPH) combined with citric acid (CA) was employed as a biphasic pretreatment system to effectively deconstruct the recalcitrant structure of sugarcane bagasse (SCB) to enhance its enzymatic digestibility. The results showed that the removal of lignin was 81.9 % and the enzymatic hydrolysis yield of SCB reached 73.9 % under the pretreatment at 170 °C with 10.0 % CA. The physicochemical changes of the SCB after the pretreatment showed that the lignin removal, hemicellulose removal, the hydrophobicity, and accessibility of the pretreated SCB were positively correlated. The interactions between lignin and other components in the phenoxyethanol-citric acid (PECA) system were analyzed through molecular dynamics (MD) simulations combined with density functional theory (DFT) calculations. It is found that the lignin molecule can interact with EPH and water to preferentially solubilize in the EPH phase due to the formed high interaction energy and increased hydrogen bond formation. In the lignin-EPH system, the addition of CA increased the number of hydrogen bonds and enhanced the interaction between lignin and the three components, which was conducive to achieving a higher degree of delignification for improved enzymatic hydrolysis.

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.